This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 169.66 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 86,473 (2.4%) Reads that were too short: 4,824 (0.1%) Reads written (passing filters): 81,649 (2.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 23,090,496 bp (2.1%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 86473 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 8667 887.3 0 8667 7 15034 887.3 0 15034 8 24565 887.3 0 24565 9 25971 887.3 0 25971 10 58 887.3 0 58 11 27 887.3 0 27 12 11 887.3 0 11 13 14 887.3 0 14 14 13 887.3 0 13 15 14 887.3 0 14 16 13 887.3 0 13 17 4 887.3 0 4 18 15 887.3 0 15 19 14 887.3 0 14 20 8 887.3 0 8 21 18 887.3 0 18 22 15 887.3 0 15 23 13 887.3 0 13 24 9 887.3 0 9 25 15 887.3 0 15 26 7 887.3 0 7 27 18 887.3 0 18 28 13 887.3 0 13 29 9 887.3 0 9 30 22 887.3 0 22 31 16 887.3 0 16 32 10 887.3 0 10 33 9 887.3 0 9 34 11 887.3 0 11 35 15 887.3 0 15 36 6 887.3 0 6 37 24 887.3 0 24 38 23 887.3 0 23 39 11 887.3 0 11 40 20 887.3 0 20 41 27 887.3 0 27 42 23 887.3 0 23 43 23 887.3 0 23 44 8 887.3 0 8 45 17 887.3 0 17 46 20 887.3 0 20 47 13 887.3 0 13 48 238 887.3 0 238 49 355 887.3 0 355 50 405 887.3 0 405 51 15 887.3 0 15 52 16 887.3 0 16 53 16 887.3 0 16 54 15 887.3 0 15 55 9 887.3 0 9 56 16 887.3 0 16 57 19 887.3 0 19 58 14 887.3 0 14 59 15 887.3 0 15 60 12 887.3 0 12 61 15 887.3 0 15 62 17 887.3 0 17 63 13 887.3 0 13 64 17 887.3 0 17 65 45 887.3 0 45 66 14 887.3 0 14 67 20 887.3 0 20 68 26 887.3 0 26 69 13 887.3 0 13 70 30 887.3 0 30 71 11 887.3 0 11 72 16 887.3 0 16 73 15 887.3 0 15 74 22 887.3 0 22 75 13 887.3 0 13 76 9 887.3 0 9 77 10 887.3 0 10 78 21 887.3 0 21 79 19 887.3 0 19 80 21 887.3 0 21 81 49 887.3 0 49 82 39 887.3 0 39 83 177 887.3 0 177 84 49 887.3 0 49 85 27 887.3 0 27 86 25 887.3 0 25 87 19 887.3 0 19 88 17 887.3 0 17 89 23 887.3 0 23 90 25 887.3 0 25 91 19 887.3 0 19 92 20 887.3 0 20 93 28 887.3 0 28 94 20 887.3 0 20 95 17 887.3 0 17 96 33 887.3 0 33 97 60 887.3 0 60 98 386 887.3 0 386 99 23 887.3 0 23 100 17 887.3 0 17 101 13 887.3 0 13 102 11 887.3 0 11 103 11 887.3 0 11 104 22 887.3 0 22 105 21 887.3 0 21 106 16 887.3 0 16 107 19 887.3 0 19 108 14 887.3 0 14 109 24 887.3 0 24 110 17 887.3 0 17 111 10 887.3 0 10 112 16 887.3 0 16 113 50 887.3 0 50 114 26 887.3 0 26 115 60 887.3 0 60 116 17 887.3 0 17 117 16 887.3 0 16 118 64 887.3 0 64 119 43 887.3 0 43 120 34 887.3 0 34 121 25 887.3 0 25 122 24 887.3 0 24 123 18 887.3 0 18 124 15 887.3 0 15 125 18 887.3 0 18 126 13 887.3 0 13 127 30 887.3 0 30 128 20 887.3 0 20 129 29 887.3 0 29 130 18 887.3 0 18 131 17 887.3 0 17 132 22 887.3 0 22 133 15 887.3 0 15 134 18 887.3 0 18 135 18 887.3 0 18 136 60 887.3 0 60 137 45 887.3 0 45 138 31 887.3 0 31 139 15 887.3 0 15 140 11 887.3 0 11 141 40 887.3 0 40 142 30 887.3 0 30 143 17 887.3 0 17 144 20 887.3 0 20 145 30 887.3 0 30 146 33 887.3 0 33 147 154 887.3 0 154 148 50 887.3 0 50 149 24 887.3 0 24 150 30 887.3 0 30 151 20 887.3 0 20 152 67 887.3 0 67 153 37 887.3 0 37 154 50 887.3 0 50 155 83 887.3 0 83 156 32 887.3 0 32 157 73 887.3 0 73 158 38 887.3 0 38 159 17 887.3 0 17 160 7 887.3 0 7 161 20 887.3 0 20 162 20 887.3 0 20 163 19 887.3 0 19 164 21 887.3 0 21 165 22 887.3 0 22 166 12 887.3 0 12 167 24 887.3 0 24 168 25 887.3 0 25 169 14 887.3 0 14 170 22 887.3 0 22 171 13 887.3 0 13 172 18 887.3 0 18 173 17 887.3 0 17 174 11 887.3 0 11 175 23 887.3 0 23 176 24 887.3 0 24 177 26 887.3 0 26 178 26 887.3 0 26 179 32 887.3 0 32 180 27 887.3 0 27 181 101 887.3 0 101 182 70 887.3 0 70 183 54 887.3 0 54 184 17 887.3 0 17 185 24 887.3 0 24 186 27 887.3 0 27 187 17 887.3 0 17 188 21 887.3 0 21 189 38 887.3 0 38 190 36 887.3 0 36 191 161 887.3 0 161 192 51 887.3 0 51 193 169 887.3 0 169 194 79 887.3 0 79 195 320 887.3 0 320 196 413 887.3 0 413 197 315 887.3 0 315 198 17 887.3 0 17 199 16 887.3 0 16 200 18 887.3 0 18 201 23 887.3 0 23 202 21 887.3 0 21 203 19 887.3 0 19 204 10 887.3 0 10 205 11 887.3 0 11 206 10 887.3 0 10 207 25 887.3 0 25 208 17 887.3 0 17 209 33 887.3 0 33 210 16 887.3 0 16 211 7 887.3 0 7 212 10 887.3 0 10 213 32 887.3 0 32 214 27 887.3 0 27 215 51 887.3 0 51 216 58 887.3 0 58 217 22 887.3 0 22 218 30 887.3 0 30 219 20 887.3 0 20 220 12 887.3 0 12 221 20 887.3 0 20 222 18 887.3 0 18 223 17 887.3 0 17 224 19 887.3 0 19 225 14 887.3 0 14 226 20 887.3 0 20 227 19 887.3 0 19 228 22 887.3 0 22 229 17 887.3 0 17 230 25 887.3 0 25 231 33 887.3 0 33 232 24 887.3 0 24 233 24 887.3 0 24 234 43 887.3 0 43 235 76 887.3 0 76 236 108 887.3 0 108 237 27 887.3 0 27 238 37 887.3 0 37 239 30 887.3 0 30 240 45 887.3 0 45 241 45 887.3 0 45 242 28 887.3 0 28 243 129 887.3 0 129 244 108 887.3 0 108 245 115 887.3 0 115 246 30 887.3 0 30 247 38 887.3 0 38 248 48 887.3 0 48 249 59 887.3 0 59 250 29 887.3 0 29 251 85 887.3 0 85 252 49 887.3 0 49 253 60 887.3 0 60 254 44 887.3 0 44 255 33 887.3 0 33 256 62 887.3 0 62 257 38 887.3 0 38 258 29 887.3 0 29 259 78 887.3 0 78 260 57 887.3 0 57 261 92 887.3 0 92 262 72 887.3 0 72 263 68 887.3 0 68 264 30 887.3 0 30 265 30 887.3 0 30 266 11 887.3 0 11 267 22 887.3 0 22 268 27 887.3 0 27 269 43 887.3 0 43 270 47 887.3 0 47 271 43 887.3 0 43 272 27 887.3 0 27 273 21 887.3 0 21 274 21 887.3 0 21 275 31 887.3 0 31 276 28 887.3 0 28 277 23 887.3 0 23 278 31 887.3 0 31 279 21 887.3 0 21 280 24 887.3 0 24 281 38 887.3 0 38 282 46 887.3 0 46 283 29 887.3 0 29 284 54 887.3 0 54 285 39 887.3 0 39 286 91 887.3 0 91 287 77 887.3 0 77 288 96 887.3 0 96 289 204 887.3 0 204 290 71 887.3 0 71 291 131 887.3 0 131 292 182 887.3 0 182 293 44 887.3 0 44 294 47 887.3 0 47 295 42 887.3 0 42 296 65 887.3 0 65 297 144 887.3 0 144 298 155 887.3 0 155 299 151 887.3 0 151 300 128 887.3 0 128 301 45 887.3 0 45 Finished in 6.11 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 81,649 Reads with adapters: 31,506 (38.6%) Reads that were too short: 272 (0.3%) Reads written (passing filters): 31,234 (38.3%) Total basepairs processed: 23,090,496 bp Total written (filtered): 8,617,162 bp (37.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 31506 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 7 16 146 0.0 3 0 145 0 1 17 23015 0.0 3 22588 408 17 2 18 114 0.0 3 9 102 3 19 7883 0.0 3 7717 150 13 3 20 31 0.0 3 5 16 0 10 21 4 0.0 3 1 0 2 1 22 6 0.0 3 0 1 0 5 23 1 0.0 3 1 24 1 0.0 3 0 0 1 29 2 0.0 3 0 0 0 2 31 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 45 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 79 5 0.0 3 0 0 0 5 82 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 216 9 0.0 3 0 0 0 9 217 248 0.0 3 0 0 7 241 218 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.83 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 31,234 Reads with adapters: 135 (0.4%) Reads written (passing filters): 31,234 (100.0%) Total basepairs processed: 8,617,162 bp Total written (filtered): 8,616,729 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 135 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.3% C: 3.0% G: 16.3% T: 4.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 109 488.0 0 109 4 24 122.0 0 24 5 2 30.5 0 2