This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.48 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 162,124 (4.5%) Reads that were too short: 45,585 (1.3%) Reads written (passing filters): 116,539 (3.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 30,978,195 bp (2.8%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 162124 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1971 887.3 0 1971 7 37199 887.3 0 37199 8 45996 887.3 0 45996 9 99 887.3 0 99 10 206 887.3 0 206 11 54 887.3 0 54 12 49 887.3 0 49 13 48 887.3 0 48 14 57 887.3 0 57 15 57 887.3 0 57 16 52 887.3 0 52 17 49 887.3 0 49 18 59 887.3 0 59 19 63 887.3 0 63 20 54 887.3 0 54 21 57 887.3 0 57 22 35 887.3 0 35 23 62 887.3 0 62 24 47 887.3 0 47 25 60 887.3 0 60 26 64 887.3 0 64 27 59 887.3 0 59 28 61 887.3 0 61 29 80 887.3 0 80 30 72 887.3 0 72 31 43 887.3 0 43 32 60 887.3 0 60 33 84 887.3 0 84 34 51 887.3 0 51 35 45 887.3 0 45 36 61 887.3 0 61 37 52 887.3 0 52 38 59 887.3 0 59 39 72 887.3 0 72 40 64 887.3 0 64 41 51 887.3 0 51 42 89 887.3 0 89 43 89 887.3 0 89 44 66 887.3 0 66 45 44 887.3 0 44 46 123 887.3 0 123 47 84 887.3 0 84 48 57 887.3 0 57 49 103 887.3 0 103 50 71 887.3 0 71 51 55 887.3 0 55 52 82 887.3 0 82 53 99 887.3 0 99 54 74 887.3 0 74 55 77 887.3 0 77 56 77 887.3 0 77 57 86 887.3 0 86 58 95 887.3 0 95 59 57 887.3 0 57 60 97 887.3 0 97 61 84 887.3 0 84 62 186 887.3 0 186 63 86 887.3 0 86 64 1964 887.3 0 1964 65 85 887.3 0 85 66 68 887.3 0 68 67 74 887.3 0 74 68 81 887.3 0 81 69 57 887.3 0 57 70 97 887.3 0 97 71 85 887.3 0 85 72 111 887.3 0 111 73 728 887.3 0 728 74 734 887.3 0 734 75 513 887.3 0 513 76 124 887.3 0 124 77 1285 887.3 0 1285 78 1463 887.3 0 1463 79 558 887.3 0 558 80 47 887.3 0 47 81 73 887.3 0 73 82 66 887.3 0 66 83 46 887.3 0 46 84 44 887.3 0 44 85 77 887.3 0 77 86 59 887.3 0 59 87 56 887.3 0 56 88 61 887.3 0 61 89 74 887.3 0 74 90 63 887.3 0 63 91 76 887.3 0 76 92 60 887.3 0 60 93 65 887.3 0 65 94 58 887.3 0 58 95 80 887.3 0 80 96 56 887.3 0 56 97 94 887.3 0 94 98 72 887.3 0 72 99 76 887.3 0 76 100 75 887.3 0 75 101 86 887.3 0 86 102 56 887.3 0 56 103 71 887.3 0 71 104 89 887.3 0 89 105 89 887.3 0 89 106 266 887.3 0 266 107 430 887.3 0 430 108 544 887.3 0 544 109 77 887.3 0 77 110 67 887.3 0 67 111 65 887.3 0 65 112 97 887.3 0 97 113 113 887.3 0 113 114 73 887.3 0 73 115 142 887.3 0 142 116 92 887.3 0 92 117 88 887.3 0 88 118 87 887.3 0 87 119 81 887.3 0 81 120 105 887.3 0 105 121 81 887.3 0 81 122 87 887.3 0 87 123 218 887.3 0 218 124 96 887.3 0 96 125 119 887.3 0 119 126 76 887.3 0 76 127 170 887.3 0 170 128 1110 887.3 0 1110 129 708 887.3 0 708 130 1509 887.3 0 1509 131 89 887.3 0 89 132 82 887.3 0 82 133 152 887.3 0 152 134 472 887.3 0 472 135 254 887.3 0 254 136 459 887.3 0 459 137 251 887.3 0 251 138 123 887.3 0 123 139 113 887.3 0 113 140 106 887.3 0 106 141 71 887.3 0 71 142 117 887.3 0 117 143 77 887.3 0 77 144 87 887.3 0 87 145 198 887.3 0 198 146 983 887.3 0 983 147 1102 887.3 0 1102 148 638 887.3 0 638 149 131 887.3 0 131 150 71 887.3 0 71 151 56 887.3 0 56 152 56 887.3 0 56 153 71 887.3 0 71 154 94 887.3 0 94 155 236 887.3 0 236 156 279 887.3 0 279 157 462 887.3 0 462 158 90 887.3 0 90 159 89 887.3 0 89 160 85 887.3 0 85 161 76 887.3 0 76 162 73 887.3 0 73 163 60 887.3 0 60 164 149 887.3 0 149 165 132 887.3 0 132 166 158 887.3 0 158 167 76 887.3 0 76 168 56 887.3 0 56 169 68 887.3 0 68 170 64 887.3 0 64 171 66 887.3 0 66 172 43 887.3 0 43 173 48 887.3 0 48 174 52 887.3 0 52 175 54 887.3 0 54 176 35 887.3 0 35 177 56 887.3 0 56 178 40 887.3 0 40 179 61 887.3 0 61 180 73 887.3 0 73 181 52 887.3 0 52 182 55 887.3 0 55 183 61 887.3 0 61 184 165 887.3 0 165 185 175 887.3 0 175 186 137 887.3 0 137 187 95 887.3 0 95 188 65 887.3 0 65 189 44 887.3 0 44 190 43 887.3 0 43 191 57 887.3 0 57 192 80 887.3 0 80 193 63 887.3 0 63 194 69 887.3 0 69 195 483 887.3 0 483 196 443 887.3 0 443 197 195 887.3 0 195 198 73 887.3 0 73 199 78 887.3 0 78 200 70 887.3 0 70 201 72 887.3 0 72 202 53 887.3 0 53 203 80 887.3 0 80 204 61 887.3 0 61 205 74 887.3 0 74 206 80 887.3 0 80 207 50 887.3 0 50 208 56 887.3 0 56 209 85 887.3 0 85 210 79 887.3 0 79 211 45 887.3 0 45 212 70 887.3 0 70 213 58 887.3 0 58 214 60 887.3 0 60 215 224 887.3 0 224 216 207 887.3 0 207 217 134 887.3 0 134 218 101 887.3 0 101 219 111 887.3 0 111 220 70 887.3 0 70 221 57 887.3 0 57 222 77 887.3 0 77 223 205 887.3 0 205 224 79 887.3 0 79 225 83 887.3 0 83 226 57 887.3 0 57 227 72 887.3 0 72 228 69 887.3 0 69 229 88 887.3 0 88 230 111 887.3 0 111 231 156 887.3 0 156 232 146 887.3 0 146 233 249 887.3 0 249 234 332 887.3 0 332 235 415 887.3 0 415 236 5568 887.3 0 5568 237 13968 887.3 0 13968 238 14328 887.3 0 14328 239 160 887.3 0 160 240 73 887.3 0 73 241 68 887.3 0 68 242 80 887.3 0 80 243 74 887.3 0 74 244 51 887.3 0 51 245 104 887.3 0 104 246 195 887.3 0 195 247 248 887.3 0 248 248 297 887.3 0 297 249 168 887.3 0 168 250 86 887.3 0 86 251 156 887.3 0 156 252 143 887.3 0 143 253 184 887.3 0 184 254 90 887.3 0 90 255 95 887.3 0 95 256 74 887.3 0 74 257 45 887.3 0 45 258 61 887.3 0 61 259 55 887.3 0 55 260 57 887.3 0 57 261 48 887.3 0 48 262 58 887.3 0 58 263 72 887.3 0 72 264 79 887.3 0 79 265 85 887.3 0 85 266 109 887.3 0 109 267 63 887.3 0 63 268 59 887.3 0 59 269 57 887.3 0 57 270 53 887.3 0 53 271 57 887.3 0 57 272 81 887.3 0 81 273 79 887.3 0 79 274 90 887.3 0 90 275 43 887.3 0 43 276 51 887.3 0 51 277 98 887.3 0 98 278 88 887.3 0 88 279 67 887.3 0 67 280 147 887.3 0 147 281 200 887.3 0 200 282 274 887.3 0 274 283 154 887.3 0 154 284 261 887.3 0 261 285 234 887.3 0 234 286 129 887.3 0 129 287 83 887.3 0 83 288 86 887.3 0 86 289 105 887.3 0 105 290 61 887.3 0 61 291 76 887.3 0 76 292 71 887.3 0 71 293 125 887.3 0 125 294 139 887.3 0 139 295 224 887.3 0 224 296 310 887.3 0 310 297 335 887.3 0 335 298 387 887.3 0 387 299 163 887.3 0 163 300 151 887.3 0 151 301 211 887.3 0 211 Finished in 8.51 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 116,539 Reads with adapters: 37,916 (32.5%) Reads that were too short: 259 (0.2%) Reads written (passing filters): 37,657 (32.3%) Total basepairs processed: 30,978,195 bp Total written (filtered): 10,408,879 bp (33.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 37916 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 239 0.0 3 0 234 5 17 37127 0.0 3 36394 702 29 2 18 94 0.0 3 17 73 4 19 11 0.0 3 0 0 9 2 20 21 0.0 3 0 0 0 21 21 1 0.0 3 0 0 1 22 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 39 45 0.0 3 0 0 0 45 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 45 14 0.0 3 0 0 0 14 46 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 114 39 0.0 3 0 0 0 39 115 22 0.0 3 0 0 0 22 117 3 0.0 3 0 0 0 3 118 10 0.0 3 0 0 0 10 119 4 0.0 3 0 0 0 4 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 1 125 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 169 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 172 3 0.0 3 0 0 0 3 174 2 0.0 3 0 0 0 2 177 1 0.0 3 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 216 2 0.0 3 0 0 0 2 217 205 0.0 3 0 0 2 203 221 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 1 228 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.18 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 37,657 Reads with adapters: 149 (0.4%) Reads written (passing filters): 37,657 (100.0%) Total basepairs processed: 10,408,879 bp Total written (filtered): 10,408,414 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 149 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.2% C: 45.0% G: 14.8% T: 16.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 138 588.4 0 138 4 10 147.1 0 10 11 1 0.0 1 0 1