This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 170.20 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 115,943 (3.2%) Reads that were too short: 51,530 (1.4%) Reads written (passing filters): 64,413 (1.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 16,151,978 bp (1.5%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 115943 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 597 887.3 0 597 7 18978 887.3 0 18978 8 381 887.3 0 381 9 22460 887.3 0 22460 10 56 887.3 0 56 11 18 887.3 0 18 12 45 887.3 0 45 13 18 887.3 0 18 14 22 887.3 0 22 15 22 887.3 0 22 16 22 887.3 0 22 17 14 887.3 0 14 18 24 887.3 0 24 19 14 887.3 0 14 20 17 887.3 0 17 21 21 887.3 0 21 22 18 887.3 0 18 23 11 887.3 0 11 24 8 887.3 0 8 25 24 887.3 0 24 26 15 887.3 0 15 27 15 887.3 0 15 28 12 887.3 0 12 29 19 887.3 0 19 30 17 887.3 0 17 31 19 887.3 0 19 32 19 887.3 0 19 33 16 887.3 0 16 34 21 887.3 0 21 35 31 887.3 0 31 36 19 887.3 0 19 37 18 887.3 0 18 38 23 887.3 0 23 39 25 887.3 0 25 40 9 887.3 0 9 41 24 887.3 0 24 42 18 887.3 0 18 43 16 887.3 0 16 44 10 887.3 0 10 45 11 887.3 0 11 46 11 887.3 0 11 47 17 887.3 0 17 48 19 887.3 0 19 49 16 887.3 0 16 50 14 887.3 0 14 51 12 887.3 0 12 52 18 887.3 0 18 53 17 887.3 0 17 54 16 887.3 0 16 55 1540 887.3 0 1540 56 46 887.3 0 46 57 13 887.3 0 13 58 16 887.3 0 16 59 11 887.3 0 11 60 11 887.3 0 11 61 15 887.3 0 15 62 11 887.3 0 11 63 17 887.3 0 17 64 22 887.3 0 22 65 24 887.3 0 24 66 24 887.3 0 24 67 28 887.3 0 28 68 18 887.3 0 18 69 21 887.3 0 21 70 41 887.3 0 41 71 74 887.3 0 74 72 86 887.3 0 86 73 40 887.3 0 40 74 17 887.3 0 17 75 25 887.3 0 25 76 42 887.3 0 42 77 90 887.3 0 90 78 72 887.3 0 72 79 54 887.3 0 54 80 73 887.3 0 73 81 149 887.3 0 149 82 221 887.3 0 221 83 201 887.3 0 201 84 53 887.3 0 53 85 30 887.3 0 30 86 35 887.3 0 35 87 47 887.3 0 47 88 31 887.3 0 31 89 24 887.3 0 24 90 23 887.3 0 23 91 46 887.3 0 46 92 46 887.3 0 46 93 29 887.3 0 29 94 40 887.3 0 40 95 37 887.3 0 37 96 30 887.3 0 30 97 186 887.3 0 186 98 60 887.3 0 60 99 34 887.3 0 34 100 61 887.3 0 61 101 96 887.3 0 96 102 208 887.3 0 208 103 344 887.3 0 344 104 197 887.3 0 197 105 75 887.3 0 75 106 91 887.3 0 91 107 110 887.3 0 110 108 109 887.3 0 109 109 108 887.3 0 108 110 132 887.3 0 132 111 202 887.3 0 202 112 182 887.3 0 182 113 121 887.3 0 121 114 138 887.3 0 138 115 115 887.3 0 115 116 99 887.3 0 99 117 291 887.3 0 291 118 179 887.3 0 179 119 211 887.3 0 211 120 121 887.3 0 121 121 77 887.3 0 77 122 104 887.3 0 104 123 124 887.3 0 124 124 132 887.3 0 132 125 109 887.3 0 109 126 146 887.3 0 146 127 170 887.3 0 170 128 95 887.3 0 95 129 115 887.3 0 115 130 104 887.3 0 104 131 265 887.3 0 265 132 407 887.3 0 407 133 474 887.3 0 474 134 314 887.3 0 314 135 281 887.3 0 281 136 210 887.3 0 210 137 212 887.3 0 212 138 278 887.3 0 278 139 263 887.3 0 263 140 255 887.3 0 255 141 126 887.3 0 126 142 80 887.3 0 80 143 91 887.3 0 91 144 122 887.3 0 122 145 130 887.3 0 130 146 342 887.3 0 342 147 343 887.3 0 343 148 289 887.3 0 289 149 114 887.3 0 114 150 100 887.3 0 100 151 123 887.3 0 123 152 110 887.3 0 110 153 164 887.3 0 164 154 141 887.3 0 141 155 158 887.3 0 158 156 259 887.3 0 259 157 377 887.3 0 377 158 462 887.3 0 462 159 244 887.3 0 244 160 156 887.3 0 156 161 146 887.3 0 146 162 117 887.3 0 117 163 110 887.3 0 110 164 116 887.3 0 116 165 143 887.3 0 143 166 143 887.3 0 143 167 138 887.3 0 138 168 409 887.3 0 409 169 83 887.3 0 83 170 102 887.3 0 102 171 106 887.3 0 106 172 120 887.3 0 120 173 99 887.3 0 99 174 106 887.3 0 106 175 83 887.3 0 83 176 78 887.3 0 78 177 100 887.3 0 100 178 104 887.3 0 104 179 112 887.3 0 112 180 336 887.3 0 336 181 569 887.3 0 569 182 450 887.3 0 450 183 117 887.3 0 117 184 141 887.3 0 141 185 155 887.3 0 155 186 126 887.3 0 126 187 114 887.3 0 114 188 117 887.3 0 117 189 105 887.3 0 105 190 110 887.3 0 110 191 109 887.3 0 109 192 142 887.3 0 142 193 119 887.3 0 119 194 153 887.3 0 153 195 96 887.3 0 96 196 104 887.3 0 104 197 92 887.3 0 92 198 161 887.3 0 161 199 154 887.3 0 154 200 143 887.3 0 143 201 109 887.3 0 109 202 89 887.3 0 89 203 127 887.3 0 127 204 126 887.3 0 126 205 175 887.3 0 175 206 226 887.3 0 226 207 261 887.3 0 261 208 314 887.3 0 314 209 388 887.3 0 388 210 649 887.3 0 649 211 7397 887.3 0 7397 212 10128 887.3 0 10128 213 7795 887.3 0 7795 214 2821 887.3 0 2821 215 422 887.3 0 422 216 92 887.3 0 92 217 93 887.3 0 93 218 154 887.3 0 154 219 151 887.3 0 151 220 127 887.3 0 127 221 112 887.3 0 112 222 92 887.3 0 92 223 189 887.3 0 189 224 351 887.3 0 351 225 351 887.3 0 351 226 239 887.3 0 239 227 109 887.3 0 109 228 92 887.3 0 92 229 115 887.3 0 115 230 135 887.3 0 135 231 80 887.3 0 80 232 64 887.3 0 64 233 120 887.3 0 120 234 116 887.3 0 116 235 126 887.3 0 126 236 115 887.3 0 115 237 109 887.3 0 109 238 137 887.3 0 137 239 170 887.3 0 170 240 131 887.3 0 131 241 109 887.3 0 109 242 119 887.3 0 119 243 146 887.3 0 146 244 391 887.3 0 391 245 251 887.3 0 251 246 225 887.3 0 225 247 109 887.3 0 109 248 109 887.3 0 109 249 88 887.3 0 88 250 87 887.3 0 87 251 94 887.3 0 94 252 130 887.3 0 130 253 134 887.3 0 134 254 145 887.3 0 145 255 153 887.3 0 153 256 228 887.3 0 228 257 186 887.3 0 186 258 180 887.3 0 180 259 135 887.3 0 135 260 134 887.3 0 134 261 125 887.3 0 125 262 113 887.3 0 113 263 122 887.3 0 122 264 137 887.3 0 137 265 147 887.3 0 147 266 208 887.3 0 208 267 274 887.3 0 274 268 232 887.3 0 232 269 221 887.3 0 221 270 959 887.3 0 959 271 882 887.3 0 882 272 639 887.3 0 639 273 246 887.3 0 246 274 219 887.3 0 219 275 178 887.3 0 178 276 141 887.3 0 141 277 192 887.3 0 192 278 212 887.3 0 212 279 273 887.3 0 273 280 197 887.3 0 197 281 121 887.3 0 121 282 112 887.3 0 112 283 119 887.3 0 119 284 163 887.3 0 163 285 158 887.3 0 158 286 141 887.3 0 141 287 269 887.3 0 269 288 313 887.3 0 313 289 277 887.3 0 277 290 176 887.3 0 176 291 205 887.3 0 205 292 1694 887.3 0 1694 293 1814 887.3 0 1814 294 1080 887.3 0 1080 295 467 887.3 0 467 296 159 887.3 0 159 297 99 887.3 0 99 298 145 887.3 0 145 299 157 887.3 0 157 300 189 887.3 0 189 301 314 887.3 0 314 Finished in 4.83 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 64,413 Reads with adapters: 19,263 (29.9%) Reads that were too short: 33 (0.1%) Reads written (passing filters): 19,230 (29.9%) Total basepairs processed: 16,151,978 bp Total written (filtered): 5,255,528 bp (32.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 19263 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 14 0.0 3 0 0 9 3 2 19 198 0.0 3 0 171 14 13 20 18805 0.0 4 17885 738 142 26 14 21 51 0.0 4 9 39 2 0 1 22 6 0.0 4 0 3 3 23 29 0.0 4 1 0 0 22 6 24 1 0.0 4 0 0 0 0 1 31 2 0.0 4 0 0 0 0 2 33 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 1 78 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 86 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 112 0.0 4 0 0 0 0 112 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 163 2 0.0 4 0 0 0 0 2 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 1 1 228 2 0.0 4 0 0 0 0 2 229 4 0.0 4 0 0 0 0 4 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.13 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 19,230 Reads with adapters: 1,173 (6.1%) Reads written (passing filters): 19,230 (100.0%) Total basepairs processed: 5,255,528 bp Total written (filtered): 5,242,103 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1173 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.2% C: 7.6% G: 85.2% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 57 300.5 0 57 4 8 75.1 0 8 9 7 0.1 0 1 6 10 38 0.0 1 3 35 11 868 0.0 1 17 851 12 178 0.0 1 2 176 14 1 0.0 1 0 1 15 3 0.0 1 0 3 20 7 0.0 1 5 2 21 1 0.0 1 1 152 4 0.0 1 2 2 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.