This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 175.12 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 283,154 (7.8%) Reads that were too short: 165,681 (4.6%) Reads written (passing filters): 117,473 (3.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 29,537,782 bp (2.7%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 283154 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 988 887.3 0 988 7 1025 887.3 0 1025 8 34023 887.3 0 34023 9 40097 887.3 0 40097 10 184 887.3 0 184 11 133 887.3 0 133 12 80 887.3 0 80 13 132 887.3 0 132 14 153 887.3 0 153 15 77 887.3 0 77 16 78 887.3 0 78 17 144 887.3 0 144 18 764 887.3 0 764 19 73 887.3 0 73 20 80 887.3 0 80 21 103 887.3 0 103 22 54 887.3 0 54 23 69 887.3 0 69 24 102 887.3 0 102 25 52 887.3 0 52 26 73 887.3 0 73 27 69 887.3 0 69 28 48 887.3 0 48 29 56 887.3 0 56 30 82 887.3 0 82 31 75 887.3 0 75 32 60 887.3 0 60 33 64 887.3 0 64 34 76 887.3 0 76 35 59 887.3 0 59 36 64 887.3 0 64 37 86 887.3 0 86 38 160 887.3 0 160 39 70 887.3 0 70 40 64 887.3 0 64 41 83 887.3 0 83 42 104 887.3 0 104 43 114 887.3 0 114 44 82 887.3 0 82 45 91 887.3 0 91 46 160 887.3 0 160 47 136 887.3 0 136 48 1167 887.3 0 1167 49 103 887.3 0 103 50 81 887.3 0 81 51 58 887.3 0 58 52 95 887.3 0 95 53 70 887.3 0 70 54 54 887.3 0 54 55 44 887.3 0 44 56 66 887.3 0 66 57 103 887.3 0 103 58 108 887.3 0 108 59 62 887.3 0 62 60 76 887.3 0 76 61 63 887.3 0 63 62 77 887.3 0 77 63 61 887.3 0 61 64 57 887.3 0 57 65 67 887.3 0 67 66 78 887.3 0 78 67 60 887.3 0 60 68 106 887.3 0 106 69 100 887.3 0 100 70 68 887.3 0 68 71 87 887.3 0 87 72 97 887.3 0 97 73 132 887.3 0 132 74 75 887.3 0 75 75 159 887.3 0 159 76 75 887.3 0 75 77 66 887.3 0 66 78 90 887.3 0 90 79 78 887.3 0 78 80 52 887.3 0 52 81 57 887.3 0 57 82 94 887.3 0 94 83 69 887.3 0 69 84 76 887.3 0 76 85 100 887.3 0 100 86 84 887.3 0 84 87 62 887.3 0 62 88 105 887.3 0 105 89 83 887.3 0 83 90 62 887.3 0 62 91 102 887.3 0 102 92 79 887.3 0 79 93 62 887.3 0 62 94 107 887.3 0 107 95 98 887.3 0 98 96 86 887.3 0 86 97 190 887.3 0 190 98 169 887.3 0 169 99 114 887.3 0 114 100 91 887.3 0 91 101 72 887.3 0 72 102 79 887.3 0 79 103 76 887.3 0 76 104 87 887.3 0 87 105 77 887.3 0 77 106 136 887.3 0 136 107 116 887.3 0 116 108 90 887.3 0 90 109 84 887.3 0 84 110 86 887.3 0 86 111 95 887.3 0 95 112 123 887.3 0 123 113 97 887.3 0 97 114 81 887.3 0 81 115 105 887.3 0 105 116 161 887.3 0 161 117 185 887.3 0 185 118 160 887.3 0 160 119 176 887.3 0 176 120 259 887.3 0 259 121 156 887.3 0 156 122 160 887.3 0 160 123 210 887.3 0 210 124 327 887.3 0 327 125 7718 887.3 0 7718 126 2796 887.3 0 2796 127 1079 887.3 0 1079 128 94 887.3 0 94 129 92 887.3 0 92 130 95 887.3 0 95 131 111 887.3 0 111 132 99 887.3 0 99 133 86 887.3 0 86 134 130 887.3 0 130 135 175 887.3 0 175 136 198 887.3 0 198 137 130 887.3 0 130 138 119 887.3 0 119 139 113 887.3 0 113 140 79 887.3 0 79 141 116 887.3 0 116 142 88 887.3 0 88 143 156 887.3 0 156 144 92 887.3 0 92 145 259 887.3 0 259 146 335 887.3 0 335 147 255 887.3 0 255 148 149 887.3 0 149 149 106 887.3 0 106 150 114 887.3 0 114 151 100 887.3 0 100 152 108 887.3 0 108 153 124 887.3 0 124 154 150 887.3 0 150 155 168 887.3 0 168 156 126 887.3 0 126 157 177 887.3 0 177 158 214 887.3 0 214 159 207 887.3 0 207 160 111 887.3 0 111 161 121 887.3 0 121 162 123 887.3 0 123 163 145 887.3 0 145 164 146 887.3 0 146 165 167 887.3 0 167 166 170 887.3 0 170 167 176 887.3 0 176 168 108 887.3 0 108 169 104 887.3 0 104 170 148 887.3 0 148 171 239 887.3 0 239 172 214 887.3 0 214 173 1025 887.3 0 1025 174 1612 887.3 0 1612 175 1052 887.3 0 1052 176 392 887.3 0 392 177 106 887.3 0 106 178 171 887.3 0 171 179 143 887.3 0 143 180 210 887.3 0 210 181 793 887.3 0 793 182 543 887.3 0 543 183 746 887.3 0 746 184 284 887.3 0 284 185 242 887.3 0 242 186 429 887.3 0 429 187 357 887.3 0 357 188 385 887.3 0 385 189 169 887.3 0 169 190 137 887.3 0 137 191 155 887.3 0 155 192 129 887.3 0 129 193 168 887.3 0 168 194 161 887.3 0 161 195 112 887.3 0 112 196 106 887.3 0 106 197 80 887.3 0 80 198 81 887.3 0 81 199 127 887.3 0 127 200 135 887.3 0 135 201 183 887.3 0 183 202 153 887.3 0 153 203 147 887.3 0 147 204 124 887.3 0 124 205 110 887.3 0 110 206 257 887.3 0 257 207 446 887.3 0 446 208 310 887.3 0 310 209 174 887.3 0 174 210 98 887.3 0 98 211 143 887.3 0 143 212 312 887.3 0 312 213 190 887.3 0 190 214 303 887.3 0 303 215 404 887.3 0 404 216 171 887.3 0 171 217 190 887.3 0 190 218 287 887.3 0 287 219 318 887.3 0 318 220 396 887.3 0 396 221 328 887.3 0 328 222 427 887.3 0 427 223 384 887.3 0 384 224 875 887.3 0 875 225 16542 887.3 0 16542 226 16104 887.3 0 16104 227 10212 887.3 0 10212 228 308 887.3 0 308 229 1833 887.3 0 1833 230 3967 887.3 0 3967 231 4015 887.3 0 4015 232 1086 887.3 0 1086 233 248 887.3 0 248 234 181 887.3 0 181 235 196 887.3 0 196 236 160 887.3 0 160 237 150 887.3 0 150 238 134 887.3 0 134 239 132 887.3 0 132 240 134 887.3 0 134 241 158 887.3 0 158 242 143 887.3 0 143 243 196 887.3 0 196 244 178 887.3 0 178 245 257 887.3 0 257 246 351 887.3 0 351 247 250 887.3 0 250 248 377 887.3 0 377 249 208 887.3 0 208 250 542 887.3 0 542 251 616 887.3 0 616 252 757 887.3 0 757 253 844 887.3 0 844 254 920 887.3 0 920 255 359 887.3 0 359 256 141 887.3 0 141 257 134 887.3 0 134 258 165 887.3 0 165 259 172 887.3 0 172 260 157 887.3 0 157 261 138 887.3 0 138 262 148 887.3 0 148 263 194 887.3 0 194 264 262 887.3 0 262 265 415 887.3 0 415 266 292 887.3 0 292 267 283 887.3 0 283 268 226 887.3 0 226 269 236 887.3 0 236 270 222 887.3 0 222 271 399 887.3 0 399 272 351 887.3 0 351 273 410 887.3 0 410 274 460 887.3 0 460 275 1272 887.3 0 1272 276 390 887.3 0 390 277 205 887.3 0 205 278 264 887.3 0 264 279 672 887.3 0 672 280 2065 887.3 0 2065 281 2577 887.3 0 2577 282 1480 887.3 0 1480 283 437 887.3 0 437 284 839 887.3 0 839 285 1554 887.3 0 1554 286 423 887.3 0 423 287 436 887.3 0 436 288 329 887.3 0 329 289 560 887.3 0 560 290 842 887.3 0 842 291 429 887.3 0 429 292 445 887.3 0 445 293 295 887.3 0 295 294 661 887.3 0 661 295 1138 887.3 0 1138 296 1624 887.3 0 1624 297 2659 887.3 0 2659 298 3762 887.3 0 3762 299 5395 887.3 0 5395 300 8763 887.3 0 8763 301 53255 887.3 0 53255 Finished in 8.22 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 117,473 Reads with adapters: 34,908 (29.7%) Reads that were too short: 58 (0.0%) Reads written (passing filters): 34,850 (29.7%) Total basepairs processed: 29,537,782 bp Total written (filtered): 9,595,444 bp (32.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 34908 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 5 0.0 3 0 0 5 16 226 0.0 3 0 220 6 17 34149 0.0 3 33456 676 13 4 18 67 0.0 3 6 54 6 1 19 23 0.0 3 1 2 19 1 20 227 0.0 3 204 7 0 16 22 1 0.0 3 0 0 1 25 1 0.0 3 1 32 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 2 0.0 3 1 0 1 39 1 0.0 3 0 0 0 1 46 13 0.0 3 0 0 0 13 49 2 0.0 3 0 0 0 2 58 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 66 106 0.0 3 0 0 0 106 68 2 0.0 3 0 0 0 2 69 12 0.0 3 0 0 0 12 73 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 5 0.0 3 0 0 0 5 101 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 106 3 0.0 3 0 0 0 3 109 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 160 4 0.0 3 0 0 0 4 166 2 0.0 3 0 0 0 2 167 2 0.0 3 0 0 0 2 173 1 0.0 3 1 180 1 0.0 3 0 0 0 1 182 4 0.0 3 0 0 0 4 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 3 0.0 3 1 0 0 2 205 2 0.0 3 0 0 0 2 206 2 0.0 3 0 1 0 1 221 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.03 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 34,850 Reads with adapters: 520 (1.5%) Reads written (passing filters): 34,850 (100.0%) Total basepairs processed: 9,595,444 bp Total written (filtered): 9,593,671 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 520 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 54.4% C: 3.5% G: 40.4% T: 1.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 311 544.5 0 311 4 205 136.1 0 205 5 4 34.0 0 4