This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 174.36 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 107,988 (3.0%) Reads that were too short: 28,324 (0.8%) Reads written (passing filters): 79,664 (2.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 20,974,302 bp (1.9%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 107988 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1097 887.3 0 1097 7 30636 887.3 0 30636 8 25268 887.3 0 25268 9 91 887.3 0 91 10 61 887.3 0 61 11 65 887.3 0 65 12 42 887.3 0 42 13 58 887.3 0 58 14 58 887.3 0 58 15 65 887.3 0 65 16 47 887.3 0 47 17 76 887.3 0 76 18 77 887.3 0 77 19 59 887.3 0 59 20 62 887.3 0 62 21 70 887.3 0 70 22 66 887.3 0 66 23 66 887.3 0 66 24 53 887.3 0 53 25 57 887.3 0 57 26 59 887.3 0 59 27 52 887.3 0 52 28 51 887.3 0 51 29 66 887.3 0 66 30 54 887.3 0 54 31 41 887.3 0 41 32 73 887.3 0 73 33 53 887.3 0 53 34 57 887.3 0 57 35 53 887.3 0 53 36 42 887.3 0 42 37 52 887.3 0 52 38 81 887.3 0 81 39 602 887.3 0 602 40 779 887.3 0 779 41 599 887.3 0 599 42 287 887.3 0 287 43 135 887.3 0 135 44 47 887.3 0 47 45 45 887.3 0 45 46 59 887.3 0 59 47 60 887.3 0 60 48 52 887.3 0 52 49 53 887.3 0 53 50 54 887.3 0 54 51 81 887.3 0 81 52 77 887.3 0 77 53 65 887.3 0 65 54 62 887.3 0 62 55 60 887.3 0 60 56 56 887.3 0 56 57 55 887.3 0 55 58 57 887.3 0 57 59 54 887.3 0 54 60 80 887.3 0 80 61 65 887.3 0 65 62 45 887.3 0 45 63 48 887.3 0 48 64 75 887.3 0 75 65 56 887.3 0 56 66 74 887.3 0 74 67 56 887.3 0 56 68 44 887.3 0 44 69 52 887.3 0 52 70 67 887.3 0 67 71 73 887.3 0 73 72 94 887.3 0 94 73 66 887.3 0 66 74 96 887.3 0 96 75 53 887.3 0 53 76 56 887.3 0 56 77 74 887.3 0 74 78 148 887.3 0 148 79 75 887.3 0 75 80 116 887.3 0 116 81 72 887.3 0 72 82 66 887.3 0 66 83 74 887.3 0 74 84 85 887.3 0 85 85 82 887.3 0 82 86 79 887.3 0 79 87 68 887.3 0 68 88 52 887.3 0 52 89 76 887.3 0 76 90 56 887.3 0 56 91 117 887.3 0 117 92 161 887.3 0 161 93 118 887.3 0 118 94 80 887.3 0 80 95 90 887.3 0 90 96 94 887.3 0 94 97 137 887.3 0 137 98 557 887.3 0 557 99 498 887.3 0 498 100 369 887.3 0 369 101 127 887.3 0 127 102 148 887.3 0 148 103 96 887.3 0 96 104 99 887.3 0 99 105 88 887.3 0 88 106 77 887.3 0 77 107 104 887.3 0 104 108 218 887.3 0 218 109 78 887.3 0 78 110 96 887.3 0 96 111 224 887.3 0 224 112 264 887.3 0 264 113 202 887.3 0 202 114 69 887.3 0 69 115 72 887.3 0 72 116 70 887.3 0 70 117 69 887.3 0 69 118 91 887.3 0 91 119 64 887.3 0 64 120 80 887.3 0 80 121 82 887.3 0 82 122 45 887.3 0 45 123 84 887.3 0 84 124 135 887.3 0 135 125 159 887.3 0 159 126 76 887.3 0 76 127 60 887.3 0 60 128 80 887.3 0 80 129 93 887.3 0 93 130 106 887.3 0 106 131 75 887.3 0 75 132 90 887.3 0 90 133 396 887.3 0 396 134 96 887.3 0 96 135 148 887.3 0 148 136 87 887.3 0 87 137 128 887.3 0 128 138 152 887.3 0 152 139 140 887.3 0 140 140 73 887.3 0 73 141 71 887.3 0 71 142 120 887.3 0 120 143 130 887.3 0 130 144 92 887.3 0 92 145 109 887.3 0 109 146 149 887.3 0 149 147 207 887.3 0 207 148 205 887.3 0 205 149 108 887.3 0 108 150 110 887.3 0 110 151 74 887.3 0 74 152 77 887.3 0 77 153 88 887.3 0 88 154 96 887.3 0 96 155 200 887.3 0 200 156 99 887.3 0 99 157 98 887.3 0 98 158 148 887.3 0 148 159 168 887.3 0 168 160 192 887.3 0 192 161 63 887.3 0 63 162 97 887.3 0 97 163 119 887.3 0 119 164 125 887.3 0 125 165 66 887.3 0 66 166 109 887.3 0 109 167 97 887.3 0 97 168 111 887.3 0 111 169 108 887.3 0 108 170 228 887.3 0 228 171 194 887.3 0 194 172 352 887.3 0 352 173 123 887.3 0 123 174 95 887.3 0 95 175 65 887.3 0 65 176 92 887.3 0 92 177 162 887.3 0 162 178 200 887.3 0 200 179 223 887.3 0 223 180 150 887.3 0 150 181 94 887.3 0 94 182 107 887.3 0 107 183 202 887.3 0 202 184 300 887.3 0 300 185 244 887.3 0 244 186 178 887.3 0 178 187 134 887.3 0 134 188 165 887.3 0 165 189 219 887.3 0 219 190 140 887.3 0 140 191 175 887.3 0 175 192 148 887.3 0 148 193 179 887.3 0 179 194 120 887.3 0 120 195 129 887.3 0 129 196 112 887.3 0 112 197 87 887.3 0 87 198 87 887.3 0 87 199 112 887.3 0 112 200 78 887.3 0 78 201 102 887.3 0 102 202 92 887.3 0 92 203 104 887.3 0 104 204 99 887.3 0 99 205 104 887.3 0 104 206 96 887.3 0 96 207 103 887.3 0 103 208 108 887.3 0 108 209 124 887.3 0 124 210 178 887.3 0 178 211 198 887.3 0 198 212 170 887.3 0 170 213 168 887.3 0 168 214 668 887.3 0 668 215 965 887.3 0 965 216 497 887.3 0 497 217 188 887.3 0 188 218 102 887.3 0 102 219 104 887.3 0 104 220 107 887.3 0 107 221 108 887.3 0 108 222 165 887.3 0 165 223 1041 887.3 0 1041 224 804 887.3 0 804 225 1136 887.3 0 1136 226 129 887.3 0 129 227 119 887.3 0 119 228 140 887.3 0 140 229 143 887.3 0 143 230 162 887.3 0 162 231 91 887.3 0 91 232 93 887.3 0 93 233 90 887.3 0 90 234 105 887.3 0 105 235 120 887.3 0 120 236 122 887.3 0 122 237 159 887.3 0 159 238 195 887.3 0 195 239 205 887.3 0 205 240 275 887.3 0 275 241 227 887.3 0 227 242 238 887.3 0 238 243 150 887.3 0 150 244 97 887.3 0 97 245 106 887.3 0 106 246 105 887.3 0 105 247 101 887.3 0 101 248 108 887.3 0 108 249 167 887.3 0 167 250 201 887.3 0 201 251 209 887.3 0 209 252 121 887.3 0 121 253 112 887.3 0 112 254 147 887.3 0 147 255 157 887.3 0 157 256 240 887.3 0 240 257 238 887.3 0 238 258 184 887.3 0 184 259 144 887.3 0 144 260 148 887.3 0 148 261 145 887.3 0 145 262 151 887.3 0 151 263 540 887.3 0 540 264 723 887.3 0 723 265 465 887.3 0 465 266 97 887.3 0 97 267 125 887.3 0 125 268 94 887.3 0 94 269 110 887.3 0 110 270 176 887.3 0 176 271 392 887.3 0 392 272 1604 887.3 0 1604 273 2210 887.3 0 2210 274 2037 887.3 0 2037 275 197 887.3 0 197 276 264 887.3 0 264 277 388 887.3 0 388 278 288 887.3 0 288 279 143 887.3 0 143 280 110 887.3 0 110 281 115 887.3 0 115 282 148 887.3 0 148 283 378 887.3 0 378 284 786 887.3 0 786 285 757 887.3 0 757 286 413 887.3 0 413 287 175 887.3 0 175 288 188 887.3 0 188 289 110 887.3 0 110 290 101 887.3 0 101 291 96 887.3 0 96 292 108 887.3 0 108 293 103 887.3 0 103 294 119 887.3 0 119 295 121 887.3 0 121 296 84 887.3 0 84 297 147 887.3 0 147 298 307 887.3 0 307 299 394 887.3 0 394 300 376 887.3 0 376 301 262 887.3 0 262 Finished in 6.32 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 79,664 Reads with adapters: 26,759 (33.6%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 26,723 (33.5%) Total basepairs processed: 20,974,302 bp Total written (filtered): 7,207,236 bp (34.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26759 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 13 0.0 3 0 0 12 1 19 313 0.0 3 0 271 23 13 6 20 25356 0.0 4 24106 1005 170 51 24 21 105 0.0 4 22 74 5 1 3 22 16 0.0 4 3 0 10 3 23 75 0.0 4 1 3 1 52 18 24 2 0.0 4 1 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 1 36 32 0.0 4 1 0 0 0 31 41 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 1 79 1 0.0 4 0 0 0 0 1 82 3 0.0 4 0 0 0 0 3 89 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 95 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 8 0.0 4 0 0 0 0 8 127 788 0.0 4 0 0 0 0 788 128 2 0.0 4 0 0 0 0 2 130 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 215 2 0.0 4 0 0 0 0 2 221 1 0.0 4 0 0 0 0 1 223 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 2 2 229 3 0.0 4 0 0 0 0 3 230 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 243 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.61 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 26,723 Reads with adapters: 4,740 (17.7%) Reads written (passing filters): 26,723 (100.0%) Total basepairs processed: 7,207,236 bp Total written (filtered): 7,155,477 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4740 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.9% C: 4.9% G: 91.5% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 40 417.5 0 40 4 6 104.4 0 6 6 1 6.5 0 1 9 23 0.1 0 0 23 10 657 0.0 1 25 632 11 3972 0.0 1 80 3892 12 31 0.0 1 2 29 13 2 0.0 1 0 2 14 3 0.0 1 0 3 38 1 0.0 1 1 151 3 0.0 1 3 209 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.