This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 172.63 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 107,735 (3.0%) Reads that were too short: 24,132 (0.7%) Reads written (passing filters): 83,603 (2.3%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 22,057,677 bp (2.0%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 107735 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 893 887.3 0 893 7 26233 887.3 0 26233 8 31501 887.3 0 31501 9 107 887.3 0 107 10 85 887.3 0 85 11 83 887.3 0 83 12 93 887.3 0 93 13 92 887.3 0 92 14 106 887.3 0 106 15 83 887.3 0 83 16 79 887.3 0 79 17 82 887.3 0 82 18 72 887.3 0 72 19 99 887.3 0 99 20 96 887.3 0 96 21 111 887.3 0 111 22 86 887.3 0 86 23 69 887.3 0 69 24 64 887.3 0 64 25 84 887.3 0 84 26 73 887.3 0 73 27 87 887.3 0 87 28 83 887.3 0 83 29 80 887.3 0 80 30 77 887.3 0 77 31 97 887.3 0 97 32 99 887.3 0 99 33 73 887.3 0 73 34 65 887.3 0 65 35 106 887.3 0 106 36 86 887.3 0 86 37 76 887.3 0 76 38 122 887.3 0 122 39 463 887.3 0 463 40 521 887.3 0 521 41 748 887.3 0 748 42 272 887.3 0 272 43 127 887.3 0 127 44 69 887.3 0 69 45 69 887.3 0 69 46 114 887.3 0 114 47 79 887.3 0 79 48 42 887.3 0 42 49 79 887.3 0 79 50 85 887.3 0 85 51 80 887.3 0 80 52 68 887.3 0 68 53 84 887.3 0 84 54 87 887.3 0 87 55 73 887.3 0 73 56 61 887.3 0 61 57 103 887.3 0 103 58 90 887.3 0 90 59 71 887.3 0 71 60 108 887.3 0 108 61 69 887.3 0 69 62 59 887.3 0 59 63 64 887.3 0 64 64 67 887.3 0 67 65 119 887.3 0 119 66 107 887.3 0 107 67 1327 887.3 0 1327 68 111 887.3 0 111 69 74 887.3 0 74 70 97 887.3 0 97 71 109 887.3 0 109 72 88 887.3 0 88 73 95 887.3 0 95 74 131 887.3 0 131 75 97 887.3 0 97 76 93 887.3 0 93 77 88 887.3 0 88 78 170 887.3 0 170 79 93 887.3 0 93 80 152 887.3 0 152 81 72 887.3 0 72 82 79 887.3 0 79 83 97 887.3 0 97 84 99 887.3 0 99 85 73 887.3 0 73 86 85 887.3 0 85 87 72 887.3 0 72 88 82 887.3 0 82 89 60 887.3 0 60 90 88 887.3 0 88 91 129 887.3 0 129 92 169 887.3 0 169 93 193 887.3 0 193 94 97 887.3 0 97 95 93 887.3 0 93 96 108 887.3 0 108 97 111 887.3 0 111 98 341 887.3 0 341 99 359 887.3 0 359 100 284 887.3 0 284 101 112 887.3 0 112 102 121 887.3 0 121 103 119 887.3 0 119 104 94 887.3 0 94 105 106 887.3 0 106 106 82 887.3 0 82 107 135 887.3 0 135 108 293 887.3 0 293 109 85 887.3 0 85 110 96 887.3 0 96 111 183 887.3 0 183 112 142 887.3 0 142 113 152 887.3 0 152 114 97 887.3 0 97 115 85 887.3 0 85 116 105 887.3 0 105 117 95 887.3 0 95 118 98 887.3 0 98 119 88 887.3 0 88 120 106 887.3 0 106 121 74 887.3 0 74 122 82 887.3 0 82 123 126 887.3 0 126 124 163 887.3 0 163 125 185 887.3 0 185 126 87 887.3 0 87 127 106 887.3 0 106 128 96 887.3 0 96 129 93 887.3 0 93 130 93 887.3 0 93 131 110 887.3 0 110 132 118 887.3 0 118 133 407 887.3 0 407 134 93 887.3 0 93 135 190 887.3 0 190 136 80 887.3 0 80 137 115 887.3 0 115 138 115 887.3 0 115 139 102 887.3 0 102 140 96 887.3 0 96 141 96 887.3 0 96 142 109 887.3 0 109 143 115 887.3 0 115 144 83 887.3 0 83 145 131 887.3 0 131 146 111 887.3 0 111 147 205 887.3 0 205 148 180 887.3 0 180 149 121 887.3 0 121 150 102 887.3 0 102 151 111 887.3 0 111 152 87 887.3 0 87 153 104 887.3 0 104 154 99 887.3 0 99 155 195 887.3 0 195 156 97 887.3 0 97 157 110 887.3 0 110 158 154 887.3 0 154 159 178 887.3 0 178 160 207 887.3 0 207 161 80 887.3 0 80 162 107 887.3 0 107 163 109 887.3 0 109 164 106 887.3 0 106 165 92 887.3 0 92 166 94 887.3 0 94 167 92 887.3 0 92 168 100 887.3 0 100 169 97 887.3 0 97 170 202 887.3 0 202 171 190 887.3 0 190 172 276 887.3 0 276 173 141 887.3 0 141 174 88 887.3 0 88 175 83 887.3 0 83 176 114 887.3 0 114 177 143 887.3 0 143 178 177 887.3 0 177 179 203 887.3 0 203 180 117 887.3 0 117 181 95 887.3 0 95 182 113 887.3 0 113 183 194 887.3 0 194 184 301 887.3 0 301 185 199 887.3 0 199 186 218 887.3 0 218 187 106 887.3 0 106 188 119 887.3 0 119 189 162 887.3 0 162 190 118 887.3 0 118 191 141 887.3 0 141 192 134 887.3 0 134 193 146 887.3 0 146 194 88 887.3 0 88 195 120 887.3 0 120 196 104 887.3 0 104 197 96 887.3 0 96 198 105 887.3 0 105 199 99 887.3 0 99 200 88 887.3 0 88 201 90 887.3 0 90 202 95 887.3 0 95 203 86 887.3 0 86 204 112 887.3 0 112 205 89 887.3 0 89 206 94 887.3 0 94 207 95 887.3 0 95 208 78 887.3 0 78 209 106 887.3 0 106 210 163 887.3 0 163 211 140 887.3 0 140 212 134 887.3 0 134 213 123 887.3 0 123 214 353 887.3 0 353 215 421 887.3 0 421 216 260 887.3 0 260 217 117 887.3 0 117 218 76 887.3 0 76 219 78 887.3 0 78 220 96 887.3 0 96 221 107 887.3 0 107 222 138 887.3 0 138 223 952 887.3 0 952 224 717 887.3 0 717 225 1179 887.3 0 1179 226 93 887.3 0 93 227 84 887.3 0 84 228 116 887.3 0 116 229 150 887.3 0 150 230 141 887.3 0 141 231 75 887.3 0 75 232 83 887.3 0 83 233 96 887.3 0 96 234 82 887.3 0 82 235 96 887.3 0 96 236 99 887.3 0 99 237 116 887.3 0 116 238 182 887.3 0 182 239 168 887.3 0 168 240 207 887.3 0 207 241 184 887.3 0 184 242 172 887.3 0 172 243 144 887.3 0 144 244 82 887.3 0 82 245 100 887.3 0 100 246 77 887.3 0 77 247 82 887.3 0 82 248 84 887.3 0 84 249 143 887.3 0 143 250 172 887.3 0 172 251 158 887.3 0 158 252 106 887.3 0 106 253 94 887.3 0 94 254 119 887.3 0 119 255 135 887.3 0 135 256 157 887.3 0 157 257 132 887.3 0 132 258 121 887.3 0 121 259 110 887.3 0 110 260 122 887.3 0 122 261 128 887.3 0 128 262 140 887.3 0 140 263 553 887.3 0 553 264 794 887.3 0 794 265 572 887.3 0 572 266 90 887.3 0 90 267 106 887.3 0 106 268 104 887.3 0 104 269 109 887.3 0 109 270 129 887.3 0 129 271 302 887.3 0 302 272 1749 887.3 0 1749 273 2447 887.3 0 2447 274 2341 887.3 0 2341 275 184 887.3 0 184 276 123 887.3 0 123 277 170 887.3 0 170 278 153 887.3 0 153 279 121 887.3 0 121 280 110 887.3 0 110 281 91 887.3 0 91 282 106 887.3 0 106 283 217 887.3 0 217 284 402 887.3 0 402 285 452 887.3 0 452 286 287 887.3 0 287 287 123 887.3 0 123 288 151 887.3 0 151 289 113 887.3 0 113 290 99 887.3 0 99 291 79 887.3 0 79 292 64 887.3 0 64 293 99 887.3 0 99 294 84 887.3 0 84 295 85 887.3 0 85 296 78 887.3 0 78 297 104 887.3 0 104 298 198 887.3 0 198 299 301 887.3 0 301 300 307 887.3 0 307 301 176 887.3 0 176 Finished in 5.99 s (72 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 83,603 Reads with adapters: 26,203 (31.3%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 26,188 (31.3%) Total basepairs processed: 22,057,677 bp Total written (filtered): 7,245,999 bp (32.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26203 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 7 16 135 0.0 3 0 128 7 17 25897 0.0 3 25361 509 23 4 18 79 0.0 3 6 71 1 1 19 9 0.0 3 0 0 7 2 20 4 0.0 3 0 0 0 4 29 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 93 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 132 15 0.0 3 0 0 0 15 133 3 0.0 3 0 0 0 3 134 1 0.0 3 0 0 0 1 135 12 0.0 3 0 0 0 12 137 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 153 5 0.0 3 0 0 0 5 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 247 2 0.0 3 0 0 0 2 252 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.55 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 26,188 Reads with adapters: 93 (0.4%) Reads written (passing filters): 26,188 (100.0%) Total basepairs processed: 7,245,999 bp Total written (filtered): 7,245,712 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 93 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 46.2% C: 36.6% G: 11.8% T: 5.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 87 409.2 0 87 4 5 102.3 0 5 6 1 6.4 0 1