This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 177.90 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 406,302 (11.2%) Reads that were too short: 30,994 (0.9%) Reads written (passing filters): 375,308 (10.3%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 85,523,008 bp (7.8%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 406302 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2665 887.3 0 2665 7 72433 887.3 0 72433 8 2613 887.3 0 2613 9 83654 887.3 0 83654 10 278 887.3 0 278 11 223 887.3 0 223 12 158 887.3 0 158 13 124 887.3 0 124 14 151 887.3 0 151 15 161 887.3 0 161 16 155 887.3 0 155 17 182 887.3 0 182 18 152 887.3 0 152 19 126 887.3 0 126 20 181 887.3 0 181 21 567 887.3 0 567 22 422 887.3 0 422 23 374 887.3 0 374 24 1370 887.3 0 1370 25 1754 887.3 0 1754 26 1122 887.3 0 1122 27 154 887.3 0 154 28 160 887.3 0 160 29 151 887.3 0 151 30 121 887.3 0 121 31 136 887.3 0 136 32 150 887.3 0 150 33 154 887.3 0 154 34 122 887.3 0 122 35 159 887.3 0 159 36 151 887.3 0 151 37 133 887.3 0 133 38 104 887.3 0 104 39 148 887.3 0 148 40 121 887.3 0 121 41 137 887.3 0 137 42 114 887.3 0 114 43 117 887.3 0 117 44 145 887.3 0 145 45 143 887.3 0 143 46 139 887.3 0 139 47 168 887.3 0 168 48 150 887.3 0 150 49 136 887.3 0 136 50 130 887.3 0 130 51 134 887.3 0 134 52 128 887.3 0 128 53 161 887.3 0 161 54 181 887.3 0 181 55 188 887.3 0 188 56 277 887.3 0 277 57 259 887.3 0 259 58 162 887.3 0 162 59 162 887.3 0 162 60 180 887.3 0 180 61 787 887.3 0 787 62 981 887.3 0 981 63 682 887.3 0 682 64 128 887.3 0 128 65 161 887.3 0 161 66 179 887.3 0 179 67 156 887.3 0 156 68 231 887.3 0 231 69 364 887.3 0 364 70 464 887.3 0 464 71 323 887.3 0 323 72 192 887.3 0 192 73 269 887.3 0 269 74 254 887.3 0 254 75 281 887.3 0 281 76 180 887.3 0 180 77 177 887.3 0 177 78 377 887.3 0 377 79 285 887.3 0 285 80 397 887.3 0 397 81 292 887.3 0 292 82 541 887.3 0 541 83 3399 887.3 0 3399 84 4791 887.3 0 4791 85 2046 887.3 0 2046 86 245 887.3 0 245 87 184 887.3 0 184 88 340 887.3 0 340 89 185 887.3 0 185 90 179 887.3 0 179 91 184 887.3 0 184 92 195 887.3 0 195 93 166 887.3 0 166 94 210 887.3 0 210 95 167 887.3 0 167 96 191 887.3 0 191 97 279 887.3 0 279 98 275 887.3 0 275 99 499 887.3 0 499 100 198 887.3 0 198 101 246 887.3 0 246 102 334 887.3 0 334 103 292 887.3 0 292 104 281 887.3 0 281 105 435 887.3 0 435 106 399 887.3 0 399 107 494 887.3 0 494 108 484 887.3 0 484 109 651 887.3 0 651 110 987 887.3 0 987 111 2344 887.3 0 2344 112 39544 887.3 0 39544 113 32960 887.3 0 32960 114 10376 887.3 0 10376 115 262 887.3 0 262 116 209 887.3 0 209 117 237 887.3 0 237 118 317 887.3 0 317 119 518 887.3 0 518 120 4385 887.3 0 4385 121 3401 887.3 0 3401 122 2418 887.3 0 2418 123 449 887.3 0 449 124 522 887.3 0 522 125 2195 887.3 0 2195 126 9985 887.3 0 9985 127 489 887.3 0 489 128 197 887.3 0 197 129 550 887.3 0 550 130 339 887.3 0 339 131 421 887.3 0 421 132 181 887.3 0 181 133 191 887.3 0 191 134 246 887.3 0 246 135 248 887.3 0 248 136 358 887.3 0 358 137 392 887.3 0 392 138 294 887.3 0 294 139 252 887.3 0 252 140 259 887.3 0 259 141 314 887.3 0 314 142 672 887.3 0 672 143 628 887.3 0 628 144 694 887.3 0 694 145 401 887.3 0 401 146 489 887.3 0 489 147 3665 887.3 0 3665 148 6933 887.3 0 6933 149 2942 887.3 0 2942 150 616 887.3 0 616 151 329 887.3 0 329 152 326 887.3 0 326 153 249 887.3 0 249 154 192 887.3 0 192 155 186 887.3 0 186 156 222 887.3 0 222 157 346 887.3 0 346 158 234 887.3 0 234 159 329 887.3 0 329 160 299 887.3 0 299 161 282 887.3 0 282 162 344 887.3 0 344 163 386 887.3 0 386 164 428 887.3 0 428 165 479 887.3 0 479 166 1346 887.3 0 1346 167 1590 887.3 0 1590 168 1767 887.3 0 1767 169 1036 887.3 0 1036 170 1316 887.3 0 1316 171 3342 887.3 0 3342 172 3603 887.3 0 3603 173 2583 887.3 0 2583 174 623 887.3 0 623 175 402 887.3 0 402 176 269 887.3 0 269 177 199 887.3 0 199 178 248 887.3 0 248 179 212 887.3 0 212 180 190 887.3 0 190 181 279 887.3 0 279 182 349 887.3 0 349 183 1434 887.3 0 1434 184 1949 887.3 0 1949 185 1578 887.3 0 1578 186 707 887.3 0 707 187 764 887.3 0 764 188 586 887.3 0 586 189 440 887.3 0 440 190 276 887.3 0 276 191 1823 887.3 0 1823 192 3051 887.3 0 3051 193 2952 887.3 0 2952 194 1350 887.3 0 1350 195 389 887.3 0 389 196 394 887.3 0 394 197 272 887.3 0 272 198 254 887.3 0 254 199 388 887.3 0 388 200 352 887.3 0 352 201 394 887.3 0 394 202 318 887.3 0 318 203 146 887.3 0 146 204 153 887.3 0 153 205 197 887.3 0 197 206 235 887.3 0 235 207 250 887.3 0 250 208 285 887.3 0 285 209 186 887.3 0 186 210 230 887.3 0 230 211 189 887.3 0 189 212 168 887.3 0 168 213 194 887.3 0 194 214 198 887.3 0 198 215 229 887.3 0 229 216 263 887.3 0 263 217 245 887.3 0 245 218 233 887.3 0 233 219 254 887.3 0 254 220 205 887.3 0 205 221 163 887.3 0 163 222 207 887.3 0 207 223 190 887.3 0 190 224 253 887.3 0 253 225 521 887.3 0 521 226 784 887.3 0 784 227 608 887.3 0 608 228 834 887.3 0 834 229 1077 887.3 0 1077 230 656 887.3 0 656 231 178 887.3 0 178 232 199 887.3 0 199 233 171 887.3 0 171 234 178 887.3 0 178 235 175 887.3 0 175 236 171 887.3 0 171 237 193 887.3 0 193 238 217 887.3 0 217 239 196 887.3 0 196 240 227 887.3 0 227 241 185 887.3 0 185 242 193 887.3 0 193 243 359 887.3 0 359 244 185 887.3 0 185 245 290 887.3 0 290 246 387 887.3 0 387 247 361 887.3 0 361 248 256 887.3 0 256 249 159 887.3 0 159 250 127 887.3 0 127 251 373 887.3 0 373 252 389 887.3 0 389 253 242 887.3 0 242 254 196 887.3 0 196 255 258 887.3 0 258 256 283 887.3 0 283 257 231 887.3 0 231 258 205 887.3 0 205 259 205 887.3 0 205 260 229 887.3 0 229 261 228 887.3 0 228 262 208 887.3 0 208 263 185 887.3 0 185 264 220 887.3 0 220 265 204 887.3 0 204 266 228 887.3 0 228 267 1137 887.3 0 1137 268 2265 887.3 0 2265 269 979 887.3 0 979 270 220 887.3 0 220 271 191 887.3 0 191 272 158 887.3 0 158 273 132 887.3 0 132 274 173 887.3 0 173 275 251 887.3 0 251 276 220 887.3 0 220 277 511 887.3 0 511 278 344 887.3 0 344 279 254 887.3 0 254 280 210 887.3 0 210 281 248 887.3 0 248 282 280 887.3 0 280 283 251 887.3 0 251 284 187 887.3 0 187 285 147 887.3 0 147 286 168 887.3 0 168 287 158 887.3 0 158 288 166 887.3 0 166 289 174 887.3 0 174 290 178 887.3 0 178 291 175 887.3 0 175 292 173 887.3 0 173 293 208 887.3 0 208 294 237 887.3 0 237 295 250 887.3 0 250 296 218 887.3 0 218 297 528 887.3 0 528 298 692 887.3 0 692 299 1349 887.3 0 1349 300 212 887.3 0 212 301 188 887.3 0 188 Finished in 26.50 s (71 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 375,308 Reads with adapters: 75,024 (20.0%) Reads that were too short: 157 (0.0%) Reads written (passing filters): 74,867 (19.9%) Total basepairs processed: 85,523,008 bp Total written (filtered): 20,348,255 bp (23.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 75024 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 6 0.0 3 0 0 0 4 2 18 31 0.0 3 0 0 27 3 1 19 750 0.0 3 0 641 58 32 19 20 72060 0.0 4 68687 2811 405 122 35 21 310 0.0 4 120 175 9 3 3 22 23 0.0 4 2 2 11 5 3 23 145 0.0 4 9 1 1 100 34 24 6 0.0 4 0 0 1 1 4 25 6 0.0 4 1 0 0 0 5 26 2 0.0 4 0 0 0 1 1 27 2 0.0 4 1 0 0 0 1 28 3 0.0 4 1 0 0 0 2 31 3 0.0 4 0 0 0 0 3 36 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 41 2 0.0 4 1 0 0 0 1 46 1 0.0 4 0 0 0 0 1 49 12 0.0 4 0 0 0 0 12 50 3 0.0 4 0 0 0 0 3 51 256 0.0 4 0 0 0 0 256 54 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 65 4 0.0 4 0 0 0 0 4 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 0 0 1 73 2 0.0 4 0 0 0 0 2 74 3 0.0 4 0 0 0 0 3 75 4 0.0 4 0 0 0 0 4 76 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 95 2 0.0 4 0 0 0 0 2 98 3 0.0 4 0 0 0 0 3 99 2 0.0 4 0 0 0 0 2 100 2 0.0 4 0 0 0 0 2 104 1 0.0 4 0 0 0 0 1 106 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 1 110 46 0.0 4 0 0 0 0 46 111 1 0.0 4 0 0 0 0 1 112 2 0.0 4 0 0 0 0 2 113 9 0.0 4 0 0 0 0 9 115 1 0.0 4 0 0 0 0 1 116 2 0.0 4 0 0 0 0 2 118 2 0.0 4 0 0 0 0 2 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 1 121 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 1 0.0 4 0 0 0 0 1 126 12 0.0 4 0 0 0 0 12 127 1168 0.0 4 0 0 0 0 1168 128 6 0.0 4 0 0 0 1 5 129 5 0.0 4 1 0 0 0 4 130 4 0.0 4 0 0 0 0 4 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 1 143 1 0.0 4 0 0 0 0 1 149 2 0.0 4 0 0 0 0 2 150 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 153 3 0.0 4 0 0 0 0 3 158 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 177 10 0.0 4 0 0 0 0 10 178 4 0.0 4 0 0 0 0 4 182 1 0.0 4 0 0 0 0 1 189 2 0.0 4 0 0 0 0 2 191 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 206 2 0.0 4 0 0 0 0 2 207 1 0.0 4 0 0 0 0 1 208 4 0.0 4 0 0 0 0 4 209 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 211 2 0.0 4 0 0 0 1 1 212 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 217 1 0.0 4 0 0 0 0 1 218 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 228 11 0.0 4 0 0 0 0 11 229 7 0.0 4 0 0 0 1 6 234 2 0.0 4 0 0 0 0 2 236 1 0.0 4 0 0 0 0 1 247 2 0.0 4 0 0 0 0 2 256 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 268 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.40 s (59 us/read; 1.02 M reads/minute). === Summary === Total reads processed: 74,867 Reads with adapters: 4,975 (6.6%) Reads written (passing filters): 74,867 (100.0%) Total basepairs processed: 20,348,255 bp Total written (filtered): 20,290,690 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4975 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.2% C: 7.5% G: 85.6% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 273 1169.8 0 273 4 43 292.4 0 43 5 10 73.1 0 10 9 20 0.3 0 5 15 10 76 0.1 1 1 75 11 3962 0.0 1 57 3905 12 530 0.0 1 21 509 13 19 0.0 1 0 19 14 1 0.0 1 0 1 15 8 0.0 1 0 8 16 3 0.0 1 0 3 68 1 0.0 1 1 139 1 0.0 1 1 152 17 0.0 1 11 6 191 1 0.0 1 0 1 210 5 0.0 1 4 1 222 1 0.0 1 0 1 230 1 0.0 1 1 234 1 0.0 1 1 236 1 0.0 1 1 259 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.