This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGCAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 176.76 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 371,892 (10.2%) Reads that were too short: 28,470 (0.8%) Reads written (passing filters): 343,422 (9.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 80,762,886 bp (7.4%) === Adapter 1 === Sequence: AAGCAG; Type: regular 5'; Length: 6; Trimmed: 371892 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1925 887.3 0 1925 7 90522 887.3 0 90522 8 1741 887.3 0 1741 9 71785 887.3 0 71785 10 243 887.3 0 243 11 216 887.3 0 216 12 177 887.3 0 177 13 182 887.3 0 182 14 191 887.3 0 191 15 190 887.3 0 190 16 170 887.3 0 170 17 204 887.3 0 204 18 184 887.3 0 184 19 153 887.3 0 153 20 203 887.3 0 203 21 258 887.3 0 258 22 287 887.3 0 287 23 236 887.3 0 236 24 338 887.3 0 338 25 657 887.3 0 657 26 643 887.3 0 643 27 174 887.3 0 174 28 187 887.3 0 187 29 176 887.3 0 176 30 161 887.3 0 161 31 169 887.3 0 169 32 187 887.3 0 187 33 198 887.3 0 198 34 141 887.3 0 141 35 173 887.3 0 173 36 172 887.3 0 172 37 170 887.3 0 170 38 159 887.3 0 159 39 190 887.3 0 190 40 167 887.3 0 167 41 186 887.3 0 186 42 146 887.3 0 146 43 175 887.3 0 175 44 196 887.3 0 196 45 212 887.3 0 212 46 167 887.3 0 167 47 177 887.3 0 177 48 164 887.3 0 164 49 194 887.3 0 194 50 174 887.3 0 174 51 207 887.3 0 207 52 162 887.3 0 162 53 286 887.3 0 286 54 212 887.3 0 212 55 200 887.3 0 200 56 258 887.3 0 258 57 274 887.3 0 274 58 189 887.3 0 189 59 169 887.3 0 169 60 186 887.3 0 186 61 525 887.3 0 525 62 592 887.3 0 592 63 527 887.3 0 527 64 148 887.3 0 148 65 154 887.3 0 154 66 183 887.3 0 183 67 173 887.3 0 173 68 258 887.3 0 258 69 296 887.3 0 296 70 305 887.3 0 305 71 235 887.3 0 235 72 178 887.3 0 178 73 148 887.3 0 148 74 248 887.3 0 248 75 169 887.3 0 169 76 170 887.3 0 170 77 185 887.3 0 185 78 376 887.3 0 376 79 261 887.3 0 261 80 313 887.3 0 313 81 227 887.3 0 227 82 323 887.3 0 323 83 1226 887.3 0 1226 84 1591 887.3 0 1591 85 766 887.3 0 766 86 277 887.3 0 277 87 235 887.3 0 235 88 322 887.3 0 322 89 239 887.3 0 239 90 197 887.3 0 197 91 158 887.3 0 158 92 198 887.3 0 198 93 225 887.3 0 225 94 193 887.3 0 193 95 201 887.3 0 201 96 234 887.3 0 234 97 222 887.3 0 222 98 301 887.3 0 301 99 577 887.3 0 577 100 188 887.3 0 188 101 316 887.3 0 316 102 356 887.3 0 356 103 252 887.3 0 252 104 266 887.3 0 266 105 430 887.3 0 430 106 434 887.3 0 434 107 460 887.3 0 460 108 441 887.3 0 441 109 632 887.3 0 632 110 889 887.3 0 889 111 1853 887.3 0 1853 112 39818 887.3 0 39818 113 33812 887.3 0 33812 114 10630 887.3 0 10630 115 250 887.3 0 250 116 181 887.3 0 181 117 238 887.3 0 238 118 292 887.3 0 292 119 317 887.3 0 317 120 2162 887.3 0 2162 121 2345 887.3 0 2345 122 1502 887.3 0 1502 123 324 887.3 0 324 124 436 887.3 0 436 125 2019 887.3 0 2019 126 10272 887.3 0 10272 127 468 887.3 0 468 128 226 887.3 0 226 129 548 887.3 0 548 130 366 887.3 0 366 131 459 887.3 0 459 132 228 887.3 0 228 133 199 887.3 0 199 134 239 887.3 0 239 135 182 887.3 0 182 136 249 887.3 0 249 137 328 887.3 0 328 138 234 887.3 0 234 139 256 887.3 0 256 140 244 887.3 0 244 141 272 887.3 0 272 142 474 887.3 0 474 143 425 887.3 0 425 144 422 887.3 0 422 145 260 887.3 0 260 146 313 887.3 0 313 147 1295 887.3 0 1295 148 1394 887.3 0 1394 149 1194 887.3 0 1194 150 372 887.3 0 372 151 219 887.3 0 219 152 247 887.3 0 247 153 204 887.3 0 204 154 205 887.3 0 205 155 212 887.3 0 212 156 194 887.3 0 194 157 296 887.3 0 296 158 253 887.3 0 253 159 253 887.3 0 253 160 228 887.3 0 228 161 263 887.3 0 263 162 297 887.3 0 297 163 269 887.3 0 269 164 240 887.3 0 240 165 329 887.3 0 329 166 771 887.3 0 771 167 851 887.3 0 851 168 1002 887.3 0 1002 169 606 887.3 0 606 170 733 887.3 0 733 171 1854 887.3 0 1854 172 2268 887.3 0 2268 173 1923 887.3 0 1923 174 553 887.3 0 553 175 328 887.3 0 328 176 260 887.3 0 260 177 210 887.3 0 210 178 251 887.3 0 251 179 215 887.3 0 215 180 246 887.3 0 246 181 259 887.3 0 259 182 327 887.3 0 327 183 1539 887.3 0 1539 184 2186 887.3 0 2186 185 1569 887.3 0 1569 186 511 887.3 0 511 187 474 887.3 0 474 188 404 887.3 0 404 189 315 887.3 0 315 190 251 887.3 0 251 191 1190 887.3 0 1190 192 1799 887.3 0 1799 193 1714 887.3 0 1714 194 906 887.3 0 906 195 269 887.3 0 269 196 308 887.3 0 308 197 269 887.3 0 269 198 274 887.3 0 274 199 462 887.3 0 462 200 453 887.3 0 453 201 382 887.3 0 382 202 359 887.3 0 359 203 202 887.3 0 202 204 192 887.3 0 192 205 219 887.3 0 219 206 215 887.3 0 215 207 238 887.3 0 238 208 251 887.3 0 251 209 222 887.3 0 222 210 233 887.3 0 233 211 194 887.3 0 194 212 169 887.3 0 169 213 186 887.3 0 186 214 199 887.3 0 199 215 282 887.3 0 282 216 293 887.3 0 293 217 270 887.3 0 270 218 284 887.3 0 284 219 207 887.3 0 207 220 197 887.3 0 197 221 181 887.3 0 181 222 202 887.3 0 202 223 176 887.3 0 176 224 208 887.3 0 208 225 356 887.3 0 356 226 577 887.3 0 577 227 428 887.3 0 428 228 794 887.3 0 794 229 1083 887.3 0 1083 230 669 887.3 0 669 231 163 887.3 0 163 232 221 887.3 0 221 233 174 887.3 0 174 234 177 887.3 0 177 235 221 887.3 0 221 236 177 887.3 0 177 237 213 887.3 0 213 238 226 887.3 0 226 239 215 887.3 0 215 240 199 887.3 0 199 241 203 887.3 0 203 242 213 887.3 0 213 243 344 887.3 0 344 244 177 887.3 0 177 245 286 887.3 0 286 246 227 887.3 0 227 247 231 887.3 0 231 248 216 887.3 0 216 249 177 887.3 0 177 250 143 887.3 0 143 251 312 887.3 0 312 252 313 887.3 0 313 253 226 887.3 0 226 254 182 887.3 0 182 255 205 887.3 0 205 256 228 887.3 0 228 257 203 887.3 0 203 258 189 887.3 0 189 259 188 887.3 0 188 260 196 887.3 0 196 261 210 887.3 0 210 262 198 887.3 0 198 263 189 887.3 0 189 264 177 887.3 0 177 265 204 887.3 0 204 266 229 887.3 0 229 267 1117 887.3 0 1117 268 2370 887.3 0 2370 269 992 887.3 0 992 270 189 887.3 0 189 271 177 887.3 0 177 272 178 887.3 0 178 273 149 887.3 0 149 274 193 887.3 0 193 275 278 887.3 0 278 276 233 887.3 0 233 277 333 887.3 0 333 278 222 887.3 0 222 279 231 887.3 0 231 280 197 887.3 0 197 281 210 887.3 0 210 282 229 887.3 0 229 283 236 887.3 0 236 284 222 887.3 0 222 285 152 887.3 0 152 286 175 887.3 0 175 287 162 887.3 0 162 288 159 887.3 0 159 289 182 887.3 0 182 290 204 887.3 0 204 291 175 887.3 0 175 292 185 887.3 0 185 293 205 887.3 0 205 294 224 887.3 0 224 295 208 887.3 0 208 296 205 887.3 0 205 297 324 887.3 0 324 298 419 887.3 0 419 299 631 887.3 0 631 300 177 887.3 0 177 301 189 887.3 0 189 Finished in 22.51 s (66 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 343,422 Reads with adapters: 74,134 (21.6%) Reads that were too short: 167 (0.0%) Reads written (passing filters): 73,967 (21.5%) Total basepairs processed: 80,762,886 bp Total written (filtered): 20,294,278 bp (25.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 74134 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 11 0.0 3 0 0 10 1 16 380 0.0 3 0 368 12 17 72940 0.0 3 71291 1590 53 6 18 235 0.0 3 41 181 6 7 19 49 0.0 3 1 0 43 5 20 22 0.0 3 5 0 1 16 21 3 0.0 3 0 0 0 3 22 1 0.0 3 1 24 78 0.0 3 0 0 1 77 26 28 0.0 3 0 0 0 28 27 11 0.0 3 1 0 0 10 30 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 39 1 0.0 3 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 1 0.0 3 1 48 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 24 0.0 3 0 0 0 24 54 3 0.0 3 0 0 0 3 59 7 0.0 3 0 0 0 7 60 3 0.0 3 0 0 1 2 62 2 0.0 3 0 0 0 2 63 6 0.0 3 1 0 0 5 66 8 0.0 3 0 0 0 8 67 3 0.0 3 0 0 0 3 68 6 0.0 3 0 0 0 6 69 1 0.0 3 0 0 0 1 70 5 0.0 3 0 0 0 5 71 5 0.0 3 0 0 0 5 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 79 31 0.0 3 0 0 1 30 81 3 0.0 3 0 0 0 3 82 13 0.0 3 0 0 2 11 83 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 101 3 0.0 3 0 0 0 3 102 4 0.0 3 0 0 0 4 103 1 0.0 3 0 0 0 1 105 6 0.0 3 0 0 0 6 107 2 0.0 3 0 0 0 2 108 13 0.0 3 0 0 0 13 109 11 0.0 3 0 0 0 11 110 3 0.0 3 0 0 0 3 111 1 0.0 3 0 0 0 1 112 3 0.0 3 0 0 0 3 113 2 0.0 3 0 0 0 2 114 5 0.0 3 0 0 1 4 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 117 3 0.0 3 0 0 1 2 118 1 0.0 3 1 119 2 0.0 3 0 0 0 2 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 125 2 0.0 3 0 0 0 2 126 3 0.0 3 0 0 0 3 128 1 0.0 3 0 0 0 1 130 4 0.0 3 0 0 0 4 136 3 0.0 3 0 0 0 3 138 1 0.0 3 0 0 0 1 139 5 0.0 3 0 0 0 5 140 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 147 3 0.0 3 0 0 0 3 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 34 0.0 3 0 0 0 34 164 3 0.0 3 0 0 0 3 166 2 0.0 3 0 0 0 2 167 8 0.0 3 0 0 1 7 168 10 0.0 3 0 0 1 9 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 26 0.0 3 0 0 2 24 182 1 0.0 3 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 0 1 220 4 0.0 3 0 0 1 3 222 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 1 233 3 0.0 3 0 0 0 3 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.38 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 73,967 Reads with adapters: 2,022 (2.7%) Reads written (passing filters): 73,967 (100.0%) Total basepairs processed: 20,294,278 bp Total written (filtered): 20,288,134 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2022 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 72.5% C: 5.1% G: 21.6% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1952 1155.7 0 1952 4 69 288.9 0 69 12 1 0.0 1 0 1