This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 172.32 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 250,487 (6.9%) Reads that were too short: 58,468 (1.6%) Reads written (passing filters): 192,019 (5.3%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 53,094,427 bp (4.9%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 250487 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 808 887.3 0 808 7 2000 887.3 0 2000 8 93542 887.3 0 93542 9 65460 887.3 0 65460 10 171 887.3 0 171 11 109 887.3 0 109 12 145 887.3 0 145 13 99 887.3 0 99 14 130 887.3 0 130 15 116 887.3 0 116 16 123 887.3 0 123 17 107 887.3 0 107 18 130 887.3 0 130 19 152 887.3 0 152 20 110 887.3 0 110 21 119 887.3 0 119 22 127 887.3 0 127 23 126 887.3 0 126 24 104 887.3 0 104 25 123 887.3 0 123 26 106 887.3 0 106 27 109 887.3 0 109 28 112 887.3 0 112 29 137 887.3 0 137 30 121 887.3 0 121 31 117 887.3 0 117 32 122 887.3 0 122 33 116 887.3 0 116 34 132 887.3 0 132 35 138 887.3 0 138 36 128 887.3 0 128 37 116 887.3 0 116 38 116 887.3 0 116 39 95 887.3 0 95 40 116 887.3 0 116 41 122 887.3 0 122 42 132 887.3 0 132 43 114 887.3 0 114 44 167 887.3 0 167 45 152 887.3 0 152 46 113 887.3 0 113 47 111 887.3 0 111 48 138 887.3 0 138 49 122 887.3 0 122 50 117 887.3 0 117 51 120 887.3 0 120 52 136 887.3 0 136 53 149 887.3 0 149 54 136 887.3 0 136 55 89 887.3 0 89 56 122 887.3 0 122 57 120 887.3 0 120 58 138 887.3 0 138 59 250 887.3 0 250 60 330 887.3 0 330 61 335 887.3 0 335 62 125 887.3 0 125 63 105 887.3 0 105 64 116 887.3 0 116 65 104 887.3 0 104 66 116 887.3 0 116 67 142 887.3 0 142 68 128 887.3 0 128 69 128 887.3 0 128 70 172 887.3 0 172 71 165 887.3 0 165 72 187 887.3 0 187 73 148 887.3 0 148 74 112 887.3 0 112 75 135 887.3 0 135 76 196 887.3 0 196 77 277 887.3 0 277 78 302 887.3 0 302 79 145 887.3 0 145 80 156 887.3 0 156 81 155 887.3 0 155 82 149 887.3 0 149 83 151 887.3 0 151 84 164 887.3 0 164 85 200 887.3 0 200 86 132 887.3 0 132 87 148 887.3 0 148 88 144 887.3 0 144 89 139 887.3 0 139 90 159 887.3 0 159 91 161 887.3 0 161 92 186 887.3 0 186 93 134 887.3 0 134 94 144 887.3 0 144 95 161 887.3 0 161 96 114 887.3 0 114 97 135 887.3 0 135 98 161 887.3 0 161 99 126 887.3 0 126 100 146 887.3 0 146 101 129 887.3 0 129 102 137 887.3 0 137 103 106 887.3 0 106 104 126 887.3 0 126 105 144 887.3 0 144 106 161 887.3 0 161 107 140 887.3 0 140 108 117 887.3 0 117 109 136 887.3 0 136 110 240 887.3 0 240 111 261 887.3 0 261 112 238 887.3 0 238 113 143 887.3 0 143 114 174 887.3 0 174 115 116 887.3 0 116 116 143 887.3 0 143 117 181 887.3 0 181 118 618 887.3 0 618 119 160 887.3 0 160 120 120 887.3 0 120 121 132 887.3 0 132 122 123 887.3 0 123 123 124 887.3 0 124 124 132 887.3 0 132 125 160 887.3 0 160 126 158 887.3 0 158 127 138 887.3 0 138 128 147 887.3 0 147 129 168 887.3 0 168 130 192 887.3 0 192 131 218 887.3 0 218 132 236 887.3 0 236 133 247 887.3 0 247 134 232 887.3 0 232 135 166 887.3 0 166 136 163 887.3 0 163 137 172 887.3 0 172 138 227 887.3 0 227 139 295 887.3 0 295 140 229 887.3 0 229 141 183 887.3 0 183 142 148 887.3 0 148 143 196 887.3 0 196 144 329 887.3 0 329 145 174 887.3 0 174 146 164 887.3 0 164 147 176 887.3 0 176 148 185 887.3 0 185 149 157 887.3 0 157 150 145 887.3 0 145 151 139 887.3 0 139 152 200 887.3 0 200 153 195 887.3 0 195 154 137 887.3 0 137 155 168 887.3 0 168 156 174 887.3 0 174 157 166 887.3 0 166 158 174 887.3 0 174 159 145 887.3 0 145 160 127 887.3 0 127 161 138 887.3 0 138 162 132 887.3 0 132 163 152 887.3 0 152 164 139 887.3 0 139 165 162 887.3 0 162 166 135 887.3 0 135 167 148 887.3 0 148 168 155 887.3 0 155 169 141 887.3 0 141 170 121 887.3 0 121 171 167 887.3 0 167 172 116 887.3 0 116 173 118 887.3 0 118 174 122 887.3 0 122 175 136 887.3 0 136 176 134 887.3 0 134 177 139 887.3 0 139 178 110 887.3 0 110 179 137 887.3 0 137 180 134 887.3 0 134 181 142 887.3 0 142 182 127 887.3 0 127 183 154 887.3 0 154 184 136 887.3 0 136 185 137 887.3 0 137 186 124 887.3 0 124 187 150 887.3 0 150 188 132 887.3 0 132 189 116 887.3 0 116 190 149 887.3 0 149 191 127 887.3 0 127 192 210 887.3 0 210 193 207 887.3 0 207 194 133 887.3 0 133 195 130 887.3 0 130 196 180 887.3 0 180 197 311 887.3 0 311 198 506 887.3 0 506 199 330 887.3 0 330 200 147 887.3 0 147 201 141 887.3 0 141 202 124 887.3 0 124 203 100 887.3 0 100 204 243 887.3 0 243 205 273 887.3 0 273 206 189 887.3 0 189 207 119 887.3 0 119 208 103 887.3 0 103 209 134 887.3 0 134 210 127 887.3 0 127 211 127 887.3 0 127 212 132 887.3 0 132 213 128 887.3 0 128 214 129 887.3 0 129 215 108 887.3 0 108 216 119 887.3 0 119 217 157 887.3 0 157 218 149 887.3 0 149 219 121 887.3 0 121 220 115 887.3 0 115 221 148 887.3 0 148 222 159 887.3 0 159 223 304 887.3 0 304 224 344 887.3 0 344 225 184 887.3 0 184 226 155 887.3 0 155 227 131 887.3 0 131 228 141 887.3 0 141 229 136 887.3 0 136 230 157 887.3 0 157 231 148 887.3 0 148 232 153 887.3 0 153 233 206 887.3 0 206 234 226 887.3 0 226 235 169 887.3 0 169 236 164 887.3 0 164 237 197 887.3 0 197 238 212 887.3 0 212 239 201 887.3 0 201 240 278 887.3 0 278 241 214 887.3 0 214 242 273 887.3 0 273 243 233 887.3 0 233 244 244 887.3 0 244 245 305 887.3 0 305 246 297 887.3 0 297 247 283 887.3 0 283 248 136 887.3 0 136 249 150 887.3 0 150 250 123 887.3 0 123 251 135 887.3 0 135 252 164 887.3 0 164 253 171 887.3 0 171 254 179 887.3 0 179 255 222 887.3 0 222 256 223 887.3 0 223 257 245 887.3 0 245 258 235 887.3 0 235 259 226 887.3 0 226 260 209 887.3 0 209 261 242 887.3 0 242 262 206 887.3 0 206 263 218 887.3 0 218 264 257 887.3 0 257 265 946 887.3 0 946 266 1512 887.3 0 1512 267 931 887.3 0 931 268 565 887.3 0 565 269 122 887.3 0 122 270 147 887.3 0 147 271 233 887.3 0 233 272 280 887.3 0 280 273 247 887.3 0 247 274 302 887.3 0 302 275 240 887.3 0 240 276 157 887.3 0 157 277 196 887.3 0 196 278 229 887.3 0 229 279 232 887.3 0 232 280 220 887.3 0 220 281 252 887.3 0 252 282 227 887.3 0 227 283 202 887.3 0 202 284 266 887.3 0 266 285 238 887.3 0 238 286 295 887.3 0 295 287 218 887.3 0 218 288 267 887.3 0 267 289 262 887.3 0 262 290 303 887.3 0 303 291 403 887.3 0 403 292 424 887.3 0 424 293 460 887.3 0 460 294 461 887.3 0 461 295 449 887.3 0 449 296 607 887.3 0 607 297 1732 887.3 0 1732 298 8211 887.3 0 8211 299 10837 887.3 0 10837 300 9647 887.3 0 9647 301 4448 887.3 0 4448 Finished in 15.28 s (80 us/read; 0.75 M reads/minute). === Summary === Total reads processed: 192,019 Reads with adapters: 71,169 (37.1%) Reads that were too short: 74 (0.0%) Reads written (passing filters): 71,095 (37.0%) Total basepairs processed: 53,094,427 bp Total written (filtered): 19,201,504 bp (36.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 71169 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 2 2 18 41 0.0 3 0 0 32 7 2 19 1034 0.0 3 0 953 55 17 9 20 68303 0.0 4 65474 2322 369 101 37 21 231 0.0 4 43 164 16 3 5 22 14 0.0 4 3 0 9 1 1 23 122 0.0 4 3 1 2 80 36 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 0 0 0 1 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 1 36 1 0.0 4 1 40 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 1 58 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 118 1 0.0 4 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 14 0.0 4 0 0 0 0 14 127 1307 0.0 4 0 0 0 4 1303 128 1 0.0 4 0 0 0 0 1 129 2 0.0 4 0 0 0 0 2 130 5 0.0 4 0 0 0 0 5 131 1 0.0 4 0 1 132 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 183 1 0.0 4 1 185 2 0.0 4 1 1 188 2 0.0 4 0 0 1 0 1 196 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 0 2 215 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 1 228 11 0.0 4 1 0 0 0 10 229 7 0.0 4 0 0 0 0 7 230 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 1 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 3 0.0 4 0 0 0 0 3 257 2 0.0 4 0 0 0 0 2 259 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 272 2 0.0 4 0 0 0 1 1 274 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 283 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 288 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.42 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 71,095 Reads with adapters: 18,722 (26.3%) Reads written (passing filters): 71,095 (100.0%) Total basepairs processed: 19,201,504 bp Total written (filtered): 19,014,873 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 18722 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 4.6% G: 92.7% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 1110.9 0 15 4 12 277.7 0 12 5 2 69.4 0 2 6 6 17.4 0 6 7 7 4.3 0 7 8 9 1.1 0 9 9 8680 0.3 0 379 8301 10 8102 0.1 1 446 7656 11 1770 0.0 1 32 1738 12 33 0.0 1 1 32 13 27 0.0 1 0 27 14 2 0.0 1 0 2 18 1 0.0 1 1 19 5 0.0 1 4 1 21 1 0.0 1 0 1 22 4 0.0 1 3 1 23 1 0.0 1 0 1 37 1 0.0 1 1 42 1 0.0 1 1 102 1 0.0 1 1 104 2 0.0 1 1 1 105 5 0.0 1 3 2 106 9 0.0 1 5 4 107 1 0.0 1 0 1 108 3 0.0 1 0 3 150 5 0.0 1 3 2 208 7 0.0 1 6 1 220 1 0.0 1 1 223 7 0.0 1 7 226 1 0.0 1 1 229 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.