This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 171.36 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 253,067 (7.0%) Reads that were too short: 63,918 (1.8%) Reads written (passing filters): 189,149 (5.2%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 53,024,106 bp (4.8%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 253067 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 636 887.3 0 636 7 1544 887.3 0 1544 8 80198 887.3 0 80198 9 83967 887.3 0 83967 10 169 887.3 0 169 11 91 887.3 0 91 12 110 887.3 0 110 13 78 887.3 0 78 14 95 887.3 0 95 15 97 887.3 0 97 16 96 887.3 0 96 17 104 887.3 0 104 18 118 887.3 0 118 19 105 887.3 0 105 20 83 887.3 0 83 21 109 887.3 0 109 22 99 887.3 0 99 23 99 887.3 0 99 24 102 887.3 0 102 25 123 887.3 0 123 26 61 887.3 0 61 27 94 887.3 0 94 28 66 887.3 0 66 29 79 887.3 0 79 30 112 887.3 0 112 31 80 887.3 0 80 32 68 887.3 0 68 33 91 887.3 0 91 34 95 887.3 0 95 35 87 887.3 0 87 36 84 887.3 0 84 37 87 887.3 0 87 38 79 887.3 0 79 39 75 887.3 0 75 40 93 887.3 0 93 41 81 887.3 0 81 42 94 887.3 0 94 43 97 887.3 0 97 44 108 887.3 0 108 45 124 887.3 0 124 46 99 887.3 0 99 47 93 887.3 0 93 48 88 887.3 0 88 49 102 887.3 0 102 50 100 887.3 0 100 51 99 887.3 0 99 52 93 887.3 0 93 53 92 887.3 0 92 54 103 887.3 0 103 55 106 887.3 0 106 56 96 887.3 0 96 57 83 887.3 0 83 58 97 887.3 0 97 59 259 887.3 0 259 60 321 887.3 0 321 61 385 887.3 0 385 62 74 887.3 0 74 63 108 887.3 0 108 64 82 887.3 0 82 65 72 887.3 0 72 66 80 887.3 0 80 67 92 887.3 0 92 68 103 887.3 0 103 69 98 887.3 0 98 70 160 887.3 0 160 71 126 887.3 0 126 72 204 887.3 0 204 73 108 887.3 0 108 74 104 887.3 0 104 75 101 887.3 0 101 76 111 887.3 0 111 77 156 887.3 0 156 78 210 887.3 0 210 79 104 887.3 0 104 80 112 887.3 0 112 81 141 887.3 0 141 82 104 887.3 0 104 83 91 887.3 0 91 84 95 887.3 0 95 85 120 887.3 0 120 86 87 887.3 0 87 87 95 887.3 0 95 88 85 887.3 0 85 89 100 887.3 0 100 90 113 887.3 0 113 91 114 887.3 0 114 92 126 887.3 0 126 93 125 887.3 0 125 94 122 887.3 0 122 95 115 887.3 0 115 96 86 887.3 0 86 97 91 887.3 0 91 98 106 887.3 0 106 99 110 887.3 0 110 100 118 887.3 0 118 101 111 887.3 0 111 102 96 887.3 0 96 103 106 887.3 0 106 104 84 887.3 0 84 105 96 887.3 0 96 106 110 887.3 0 110 107 83 887.3 0 83 108 101 887.3 0 101 109 115 887.3 0 115 110 123 887.3 0 123 111 176 887.3 0 176 112 159 887.3 0 159 113 121 887.3 0 121 114 119 887.3 0 119 115 91 887.3 0 91 116 114 887.3 0 114 117 134 887.3 0 134 118 598 887.3 0 598 119 127 887.3 0 127 120 97 887.3 0 97 121 93 887.3 0 93 122 101 887.3 0 101 123 112 887.3 0 112 124 110 887.3 0 110 125 86 887.3 0 86 126 108 887.3 0 108 127 96 887.3 0 96 128 119 887.3 0 119 129 104 887.3 0 104 130 162 887.3 0 162 131 134 887.3 0 134 132 156 887.3 0 156 133 183 887.3 0 183 134 187 887.3 0 187 135 114 887.3 0 114 136 93 887.3 0 93 137 97 887.3 0 97 138 177 887.3 0 177 139 325 887.3 0 325 140 184 887.3 0 184 141 105 887.3 0 105 142 135 887.3 0 135 143 150 887.3 0 150 144 297 887.3 0 297 145 133 887.3 0 133 146 118 887.3 0 118 147 119 887.3 0 119 148 119 887.3 0 119 149 117 887.3 0 117 150 72 887.3 0 72 151 95 887.3 0 95 152 163 887.3 0 163 153 153 887.3 0 153 154 102 887.3 0 102 155 101 887.3 0 101 156 124 887.3 0 124 157 125 887.3 0 125 158 126 887.3 0 126 159 105 887.3 0 105 160 73 887.3 0 73 161 89 887.3 0 89 162 87 887.3 0 87 163 93 887.3 0 93 164 85 887.3 0 85 165 104 887.3 0 104 166 82 887.3 0 82 167 75 887.3 0 75 168 113 887.3 0 113 169 90 887.3 0 90 170 93 887.3 0 93 171 102 887.3 0 102 172 74 887.3 0 74 173 104 887.3 0 104 174 88 887.3 0 88 175 82 887.3 0 82 176 93 887.3 0 93 177 96 887.3 0 96 178 98 887.3 0 98 179 98 887.3 0 98 180 120 887.3 0 120 181 104 887.3 0 104 182 79 887.3 0 79 183 101 887.3 0 101 184 72 887.3 0 72 185 74 887.3 0 74 186 90 887.3 0 90 187 90 887.3 0 90 188 93 887.3 0 93 189 115 887.3 0 115 190 89 887.3 0 89 191 122 887.3 0 122 192 159 887.3 0 159 193 194 887.3 0 194 194 84 887.3 0 84 195 90 887.3 0 90 196 123 887.3 0 123 197 295 887.3 0 295 198 436 887.3 0 436 199 317 887.3 0 317 200 86 887.3 0 86 201 93 887.3 0 93 202 79 887.3 0 79 203 75 887.3 0 75 204 269 887.3 0 269 205 324 887.3 0 324 206 194 887.3 0 194 207 98 887.3 0 98 208 83 887.3 0 83 209 81 887.3 0 81 210 79 887.3 0 79 211 68 887.3 0 68 212 82 887.3 0 82 213 99 887.3 0 99 214 73 887.3 0 73 215 88 887.3 0 88 216 89 887.3 0 89 217 76 887.3 0 76 218 95 887.3 0 95 219 92 887.3 0 92 220 85 887.3 0 85 221 100 887.3 0 100 222 112 887.3 0 112 223 312 887.3 0 312 224 367 887.3 0 367 225 155 887.3 0 155 226 110 887.3 0 110 227 111 887.3 0 111 228 91 887.3 0 91 229 86 887.3 0 86 230 99 887.3 0 99 231 122 887.3 0 122 232 135 887.3 0 135 233 127 887.3 0 127 234 152 887.3 0 152 235 121 887.3 0 121 236 128 887.3 0 128 237 150 887.3 0 150 238 138 887.3 0 138 239 113 887.3 0 113 240 144 887.3 0 144 241 115 887.3 0 115 242 113 887.3 0 113 243 132 887.3 0 132 244 119 887.3 0 119 245 184 887.3 0 184 246 189 887.3 0 189 247 175 887.3 0 175 248 118 887.3 0 118 249 113 887.3 0 113 250 86 887.3 0 86 251 83 887.3 0 83 252 101 887.3 0 101 253 105 887.3 0 105 254 129 887.3 0 129 255 199 887.3 0 199 256 186 887.3 0 186 257 166 887.3 0 166 258 175 887.3 0 175 259 148 887.3 0 148 260 133 887.3 0 133 261 168 887.3 0 168 262 138 887.3 0 138 263 154 887.3 0 154 264 116 887.3 0 116 265 382 887.3 0 382 266 628 887.3 0 628 267 379 887.3 0 379 268 238 887.3 0 238 269 78 887.3 0 78 270 102 887.3 0 102 271 125 887.3 0 125 272 147 887.3 0 147 273 164 887.3 0 164 274 217 887.3 0 217 275 164 887.3 0 164 276 110 887.3 0 110 277 155 887.3 0 155 278 175 887.3 0 175 279 183 887.3 0 183 280 236 887.3 0 236 281 224 887.3 0 224 282 244 887.3 0 244 283 165 887.3 0 165 284 210 887.3 0 210 285 226 887.3 0 226 286 262 887.3 0 262 287 207 887.3 0 207 288 252 887.3 0 252 289 256 887.3 0 256 290 283 887.3 0 283 291 464 887.3 0 464 292 446 887.3 0 446 293 441 887.3 0 441 294 545 887.3 0 545 295 546 887.3 0 546 296 623 887.3 0 623 297 1948 887.3 0 1948 298 10893 887.3 0 10893 299 13526 887.3 0 13526 300 13290 887.3 0 13290 301 7037 887.3 0 7037 Finished in 13.92 s (74 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 189,149 Reads with adapters: 76,881 (40.6%) Reads that were too short: 50 (0.0%) Reads written (passing filters): 76,831 (40.6%) Total basepairs processed: 53,024,106 bp Total written (filtered): 21,202,725 bp (40.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 76881 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 24 0.0 3 0 0 23 1 16 346 0.0 3 0 337 8 1 17 76169 0.0 3 74631 1476 56 6 18 201 0.0 3 11 182 7 1 19 32 0.0 3 0 0 27 5 20 30 0.0 3 1 0 0 29 22 1 0.0 3 1 24 1 0.0 3 0 1 27 1 0.0 3 1 30 1 0.0 3 0 1 32 1 0.0 3 0 0 0 1 37 1 0.0 3 1 39 2 0.0 3 0 0 0 2 40 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 59 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 107 1 0.0 3 0 1 115 4 0.0 3 0 0 0 4 118 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 1 1 189 31 0.0 3 0 0 0 31 192 2 0.0 3 0 0 0 2 194 1 0.0 3 0 0 1 195 1 0.0 3 0 0 0 1 199 3 0.0 3 0 0 0 3 216 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 266 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.48 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 76,831 Reads with adapters: 422 (0.5%) Reads written (passing filters): 76,831 (100.0%) Total basepairs processed: 21,202,725 bp Total written (filtered): 21,201,267 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 422 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 47.9% C: 7.1% G: 41.9% T: 3.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 246 1200.5 0 246 4 168 300.1 0 168 5 6 75.0 0 6 6 1 18.8 0 1 12 1 0.0 1 0 1