This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 177.96 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 207,281 (5.7%) Reads that were too short: 37,744 (1.0%) Reads written (passing filters): 169,537 (4.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 46,624,336 bp (4.3%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 207281 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3325 887.3 0 3325 7 75381 887.3 0 75381 8 60867 887.3 0 60867 9 654 887.3 0 654 10 140 887.3 0 140 11 96 887.3 0 96 12 75 887.3 0 75 13 93 887.3 0 93 14 92 887.3 0 92 15 77 887.3 0 77 16 87 887.3 0 87 17 75 887.3 0 75 18 88 887.3 0 88 19 110 887.3 0 110 20 78 887.3 0 78 21 81 887.3 0 81 22 79 887.3 0 79 23 78 887.3 0 78 24 84 887.3 0 84 25 96 887.3 0 96 26 84 887.3 0 84 27 80 887.3 0 80 28 84 887.3 0 84 29 96 887.3 0 96 30 138 887.3 0 138 31 83 887.3 0 83 32 86 887.3 0 86 33 69 887.3 0 69 34 72 887.3 0 72 35 68 887.3 0 68 36 106 887.3 0 106 37 97 887.3 0 97 38 71 887.3 0 71 39 121 887.3 0 121 40 119 887.3 0 119 41 106 887.3 0 106 42 128 887.3 0 128 43 149 887.3 0 149 44 114 887.3 0 114 45 91 887.3 0 91 46 102 887.3 0 102 47 136 887.3 0 136 48 92 887.3 0 92 49 90 887.3 0 90 50 76 887.3 0 76 51 112 887.3 0 112 52 72 887.3 0 72 53 140 887.3 0 140 54 171 887.3 0 171 55 134 887.3 0 134 56 84 887.3 0 84 57 74 887.3 0 74 58 126 887.3 0 126 59 138 887.3 0 138 60 93 887.3 0 93 61 73 887.3 0 73 62 126 887.3 0 126 63 153 887.3 0 153 64 1037 887.3 0 1037 65 1044 887.3 0 1044 66 988 887.3 0 988 67 84 887.3 0 84 68 159 887.3 0 159 69 97 887.3 0 97 70 157 887.3 0 157 71 111 887.3 0 111 72 70 887.3 0 70 73 60 887.3 0 60 74 86 887.3 0 86 75 110 887.3 0 110 76 91 887.3 0 91 77 69 887.3 0 69 78 61 887.3 0 61 79 109 887.3 0 109 80 123 887.3 0 123 81 126 887.3 0 126 82 63 887.3 0 63 83 99 887.3 0 99 84 96 887.3 0 96 85 84 887.3 0 84 86 76 887.3 0 76 87 90 887.3 0 90 88 64 887.3 0 64 89 139 887.3 0 139 90 153 887.3 0 153 91 186 887.3 0 186 92 502 887.3 0 502 93 376 887.3 0 376 94 283 887.3 0 283 95 151 887.3 0 151 96 84 887.3 0 84 97 73 887.3 0 73 98 67 887.3 0 67 99 94 887.3 0 94 100 129 887.3 0 129 101 152 887.3 0 152 102 99 887.3 0 99 103 82 887.3 0 82 104 108 887.3 0 108 105 121 887.3 0 121 106 97 887.3 0 97 107 67 887.3 0 67 108 81 887.3 0 81 109 92 887.3 0 92 110 86 887.3 0 86 111 116 887.3 0 116 112 90 887.3 0 90 113 63 887.3 0 63 114 103 887.3 0 103 115 103 887.3 0 103 116 96 887.3 0 96 117 165 887.3 0 165 118 105 887.3 0 105 119 107 887.3 0 107 120 90 887.3 0 90 121 88 887.3 0 88 122 117 887.3 0 117 123 121 887.3 0 121 124 102 887.3 0 102 125 102 887.3 0 102 126 76 887.3 0 76 127 106 887.3 0 106 128 109 887.3 0 109 129 105 887.3 0 105 130 259 887.3 0 259 131 259 887.3 0 259 132 190 887.3 0 190 133 145 887.3 0 145 134 95 887.3 0 95 135 172 887.3 0 172 136 221 887.3 0 221 137 188 887.3 0 188 138 135 887.3 0 135 139 123 887.3 0 123 140 90 887.3 0 90 141 104 887.3 0 104 142 109 887.3 0 109 143 120 887.3 0 120 144 104 887.3 0 104 145 110 887.3 0 110 146 115 887.3 0 115 147 179 887.3 0 179 148 183 887.3 0 183 149 176 887.3 0 176 150 170 887.3 0 170 151 153 887.3 0 153 152 191 887.3 0 191 153 203 887.3 0 203 154 143 887.3 0 143 155 309 887.3 0 309 156 544 887.3 0 544 157 362 887.3 0 362 158 315 887.3 0 315 159 133 887.3 0 133 160 186 887.3 0 186 161 139 887.3 0 139 162 156 887.3 0 156 163 158 887.3 0 158 164 109 887.3 0 109 165 112 887.3 0 112 166 159 887.3 0 159 167 228 887.3 0 228 168 307 887.3 0 307 169 241 887.3 0 241 170 120 887.3 0 120 171 250 887.3 0 250 172 222 887.3 0 222 173 212 887.3 0 212 174 93 887.3 0 93 175 319 887.3 0 319 176 612 887.3 0 612 177 460 887.3 0 460 178 155 887.3 0 155 179 154 887.3 0 154 180 120 887.3 0 120 181 151 887.3 0 151 182 183 887.3 0 183 183 208 887.3 0 208 184 129 887.3 0 129 185 133 887.3 0 133 186 158 887.3 0 158 187 176 887.3 0 176 188 322 887.3 0 322 189 223 887.3 0 223 190 166 887.3 0 166 191 135 887.3 0 135 192 99 887.3 0 99 193 112 887.3 0 112 194 112 887.3 0 112 195 156 887.3 0 156 196 161 887.3 0 161 197 150 887.3 0 150 198 155 887.3 0 155 199 141 887.3 0 141 200 185 887.3 0 185 201 220 887.3 0 220 202 228 887.3 0 228 203 157 887.3 0 157 204 181 887.3 0 181 205 153 887.3 0 153 206 114 887.3 0 114 207 154 887.3 0 154 208 148 887.3 0 148 209 130 887.3 0 130 210 182 887.3 0 182 211 151 887.3 0 151 212 178 887.3 0 178 213 147 887.3 0 147 214 204 887.3 0 204 215 232 887.3 0 232 216 349 887.3 0 349 217 338 887.3 0 338 218 329 887.3 0 329 219 333 887.3 0 333 220 421 887.3 0 421 221 851 887.3 0 851 222 1109 887.3 0 1109 223 1147 887.3 0 1147 224 467 887.3 0 467 225 235 887.3 0 235 226 265 887.3 0 265 227 259 887.3 0 259 228 338 887.3 0 338 229 219 887.3 0 219 230 128 887.3 0 128 231 126 887.3 0 126 232 157 887.3 0 157 233 193 887.3 0 193 234 202 887.3 0 202 235 187 887.3 0 187 236 178 887.3 0 178 237 242 887.3 0 242 238 231 887.3 0 231 239 263 887.3 0 263 240 256 887.3 0 256 241 943 887.3 0 943 242 1008 887.3 0 1008 243 1038 887.3 0 1038 244 578 887.3 0 578 245 417 887.3 0 417 246 298 887.3 0 298 247 234 887.3 0 234 248 211 887.3 0 211 249 262 887.3 0 262 250 281 887.3 0 281 251 231 887.3 0 231 252 583 887.3 0 583 253 755 887.3 0 755 254 1183 887.3 0 1183 255 300 887.3 0 300 256 329 887.3 0 329 257 252 887.3 0 252 258 160 887.3 0 160 259 146 887.3 0 146 260 171 887.3 0 171 261 192 887.3 0 192 262 210 887.3 0 210 263 284 887.3 0 284 264 356 887.3 0 356 265 326 887.3 0 326 266 348 887.3 0 348 267 242 887.3 0 242 268 1326 887.3 0 1326 269 1661 887.3 0 1661 270 1690 887.3 0 1690 271 793 887.3 0 793 272 618 887.3 0 618 273 382 887.3 0 382 274 323 887.3 0 323 275 291 887.3 0 291 276 201 887.3 0 201 277 298 887.3 0 298 278 239 887.3 0 239 279 191 887.3 0 191 280 170 887.3 0 170 281 200 887.3 0 200 282 255 887.3 0 255 283 333 887.3 0 333 284 286 887.3 0 286 285 254 887.3 0 254 286 245 887.3 0 245 287 283 887.3 0 283 288 177 887.3 0 177 289 206 887.3 0 206 290 410 887.3 0 410 291 651 887.3 0 651 292 519 887.3 0 519 293 380 887.3 0 380 294 205 887.3 0 205 295 174 887.3 0 174 296 230 887.3 0 230 297 278 887.3 0 278 298 362 887.3 0 362 299 547 887.3 0 547 300 590 887.3 0 590 301 456 887.3 0 456 Finished in 13.41 s (79 us/read; 0.76 M reads/minute). === Summary === Total reads processed: 169,537 Reads with adapters: 63,796 (37.6%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 63,760 (37.6%) Total basepairs processed: 46,624,336 bp Total written (filtered): 17,297,253 bp (37.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 63796 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 4 6 18 35 0.0 3 0 0 29 4 2 19 593 0.0 3 0 505 46 32 10 20 61164 0.0 4 58302 2330 401 99 32 21 189 0.0 4 33 142 6 4 4 22 21 0.0 4 3 7 9 1 1 23 682 0.0 4 503 17 5 120 37 24 7 0.0 4 2 2 0 0 3 25 1 0.0 4 1 26 1 0.0 4 0 0 0 1 27 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 35 15 0.0 4 0 0 0 0 15 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 46 2 0.0 4 1 1 50 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 80 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 1 86 1 0.0 4 0 0 0 1 91 2 0.0 4 0 0 0 0 2 92 2 0.0 4 0 0 0 0 2 108 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 124 2 0.0 4 0 0 0 0 2 125 3 0.0 4 0 0 0 0 3 126 13 0.0 4 0 0 0 0 13 127 999 0.0 4 0 0 0 0 999 128 3 0.0 4 0 0 0 0 3 129 4 0.0 4 0 0 0 0 4 130 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 227 2 0.0 4 1 0 0 0 1 228 6 0.0 4 0 0 0 1 5 229 2 0.0 4 0 0 0 1 1 234 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.92 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 63,760 Reads with adapters: 14,236 (22.3%) Reads written (passing filters): 63,760 (100.0%) Total basepairs processed: 17,297,253 bp Total written (filtered): 17,139,642 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14236 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 4.9% G: 90.9% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 57 996.2 0 57 4 30 249.1 0 30 5 2 62.3 0 2 6 2 15.6 0 2 7 3 3.9 0 3 8 1 1.0 0 1 9 150 0.2 0 2 148 10 2875 0.1 1 148 2727 11 10794 0.0 1 198 10596 12 60 0.0 1 4 56 13 12 0.0 1 0 12 14 4 0.0 1 0 4 18 2 0.0 1 1 1 19 150 0.0 1 103 47 20 36 0.0 1 31 5 21 2 0.0 1 1 1 22 2 0.0 1 0 2 23 26 0.0 1 17 9 24 4 0.0 1 2 2 25 1 0.0 1 1 67 1 0.0 1 1 115 13 0.0 1 8 5 148 1 0.0 1 1 151 5 0.0 1 1 4 191 1 0.0 1 1 209 1 0.0 1 1 227 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.