This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTCAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 178.90 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 178,355 (4.9%) Reads that were too short: 17,419 (0.5%) Reads written (passing filters): 160,936 (4.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 45,489,431 bp (4.2%) === Adapter 1 === Sequence: CGTCAC; Type: regular 5'; Length: 6; Trimmed: 178355 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2425 887.3 0 2425 7 65417 887.3 0 65417 8 75645 887.3 0 75645 9 241 887.3 0 241 10 64 887.3 0 64 11 49 887.3 0 49 12 47 887.3 0 47 13 60 887.3 0 60 14 60 887.3 0 60 15 39 887.3 0 39 16 52 887.3 0 52 17 54 887.3 0 54 18 64 887.3 0 64 19 48 887.3 0 48 20 42 887.3 0 42 21 35 887.3 0 35 22 41 887.3 0 41 23 46 887.3 0 46 24 58 887.3 0 58 25 46 887.3 0 46 26 54 887.3 0 54 27 49 887.3 0 49 28 66 887.3 0 66 29 65 887.3 0 65 30 54 887.3 0 54 31 70 887.3 0 70 32 435 887.3 0 435 33 50 887.3 0 50 34 41 887.3 0 41 35 53 887.3 0 53 36 44 887.3 0 44 37 68 887.3 0 68 38 52 887.3 0 52 39 76 887.3 0 76 40 65 887.3 0 65 41 59 887.3 0 59 42 58 887.3 0 58 43 132 887.3 0 132 44 66 887.3 0 66 45 58 887.3 0 58 46 78 887.3 0 78 47 124 887.3 0 124 48 51 887.3 0 51 49 57 887.3 0 57 50 73 887.3 0 73 51 65 887.3 0 65 52 53 887.3 0 53 53 65 887.3 0 65 54 93 887.3 0 93 55 62 887.3 0 62 56 37 887.3 0 37 57 62 887.3 0 62 58 56 887.3 0 56 59 49 887.3 0 49 60 56 887.3 0 56 61 50 887.3 0 50 62 84 887.3 0 84 63 85 887.3 0 85 64 642 887.3 0 642 65 699 887.3 0 699 66 827 887.3 0 827 67 56 887.3 0 56 68 123 887.3 0 123 69 45 887.3 0 45 70 61 887.3 0 61 71 76 887.3 0 76 72 36 887.3 0 36 73 53 887.3 0 53 74 58 887.3 0 58 75 55 887.3 0 55 76 34 887.3 0 34 77 57 887.3 0 57 78 51 887.3 0 51 79 45 887.3 0 45 80 65 887.3 0 65 81 74 887.3 0 74 82 50 887.3 0 50 83 64 887.3 0 64 84 68 887.3 0 68 85 49 887.3 0 49 86 54 887.3 0 54 87 51 887.3 0 51 88 48 887.3 0 48 89 64 887.3 0 64 90 95 887.3 0 95 91 117 887.3 0 117 92 270 887.3 0 270 93 243 887.3 0 243 94 204 887.3 0 204 95 59 887.3 0 59 96 42 887.3 0 42 97 54 887.3 0 54 98 46 887.3 0 46 99 54 887.3 0 54 100 49 887.3 0 49 101 81 887.3 0 81 102 68 887.3 0 68 103 56 887.3 0 56 104 65 887.3 0 65 105 61 887.3 0 61 106 54 887.3 0 54 107 40 887.3 0 40 108 76 887.3 0 76 109 64 887.3 0 64 110 59 887.3 0 59 111 71 887.3 0 71 112 58 887.3 0 58 113 36 887.3 0 36 114 52 887.3 0 52 115 65 887.3 0 65 116 44 887.3 0 44 117 68 887.3 0 68 118 90 887.3 0 90 119 63 887.3 0 63 120 53 887.3 0 53 121 66 887.3 0 66 122 81 887.3 0 81 123 68 887.3 0 68 124 71 887.3 0 71 125 82 887.3 0 82 126 44 887.3 0 44 127 80 887.3 0 80 128 69 887.3 0 69 129 60 887.3 0 60 130 98 887.3 0 98 131 81 887.3 0 81 132 82 887.3 0 82 133 70 887.3 0 70 134 54 887.3 0 54 135 66 887.3 0 66 136 101 887.3 0 101 137 72 887.3 0 72 138 73 887.3 0 73 139 97 887.3 0 97 140 70 887.3 0 70 141 60 887.3 0 60 142 60 887.3 0 60 143 65 887.3 0 65 144 73 887.3 0 73 145 54 887.3 0 54 146 85 887.3 0 85 147 84 887.3 0 84 148 95 887.3 0 95 149 116 887.3 0 116 150 160 887.3 0 160 151 87 887.3 0 87 152 80 887.3 0 80 153 171 887.3 0 171 154 78 887.3 0 78 155 116 887.3 0 116 156 245 887.3 0 245 157 159 887.3 0 159 158 130 887.3 0 130 159 96 887.3 0 96 160 108 887.3 0 108 161 71 887.3 0 71 162 84 887.3 0 84 163 61 887.3 0 61 164 73 887.3 0 73 165 61 887.3 0 61 166 69 887.3 0 69 167 125 887.3 0 125 168 125 887.3 0 125 169 119 887.3 0 119 170 53 887.3 0 53 171 124 887.3 0 124 172 112 887.3 0 112 173 107 887.3 0 107 174 57 887.3 0 57 175 181 887.3 0 181 176 300 887.3 0 300 177 215 887.3 0 215 178 91 887.3 0 91 179 73 887.3 0 73 180 70 887.3 0 70 181 91 887.3 0 91 182 85 887.3 0 85 183 97 887.3 0 97 184 69 887.3 0 69 185 76 887.3 0 76 186 102 887.3 0 102 187 98 887.3 0 98 188 151 887.3 0 151 189 109 887.3 0 109 190 109 887.3 0 109 191 99 887.3 0 99 192 73 887.3 0 73 193 70 887.3 0 70 194 74 887.3 0 74 195 62 887.3 0 62 196 83 887.3 0 83 197 71 887.3 0 71 198 89 887.3 0 89 199 97 887.3 0 97 200 71 887.3 0 71 201 72 887.3 0 72 202 86 887.3 0 86 203 90 887.3 0 90 204 100 887.3 0 100 205 110 887.3 0 110 206 90 887.3 0 90 207 85 887.3 0 85 208 84 887.3 0 84 209 66 887.3 0 66 210 87 887.3 0 87 211 99 887.3 0 99 212 110 887.3 0 110 213 111 887.3 0 111 214 102 887.3 0 102 215 97 887.3 0 97 216 135 887.3 0 135 217 149 887.3 0 149 218 109 887.3 0 109 219 87 887.3 0 87 220 136 887.3 0 136 221 235 887.3 0 235 222 260 887.3 0 260 223 250 887.3 0 250 224 187 887.3 0 187 225 136 887.3 0 136 226 141 887.3 0 141 227 147 887.3 0 147 228 188 887.3 0 188 229 100 887.3 0 100 230 75 887.3 0 75 231 80 887.3 0 80 232 85 887.3 0 85 233 99 887.3 0 99 234 139 887.3 0 139 235 116 887.3 0 116 236 112 887.3 0 112 237 141 887.3 0 141 238 122 887.3 0 122 239 138 887.3 0 138 240 106 887.3 0 106 241 343 887.3 0 343 242 304 887.3 0 304 243 336 887.3 0 336 244 227 887.3 0 227 245 187 887.3 0 187 246 168 887.3 0 168 247 158 887.3 0 158 248 134 887.3 0 134 249 123 887.3 0 123 250 158 887.3 0 158 251 133 887.3 0 133 252 289 887.3 0 289 253 371 887.3 0 371 254 537 887.3 0 537 255 128 887.3 0 128 256 145 887.3 0 145 257 134 887.3 0 134 258 91 887.3 0 91 259 74 887.3 0 74 260 80 887.3 0 80 261 98 887.3 0 98 262 104 887.3 0 104 263 108 887.3 0 108 264 140 887.3 0 140 265 149 887.3 0 149 266 189 887.3 0 189 267 130 887.3 0 130 268 662 887.3 0 662 269 1015 887.3 0 1015 270 663 887.3 0 663 271 217 887.3 0 217 272 200 887.3 0 200 273 175 887.3 0 175 274 205 887.3 0 205 275 161 887.3 0 161 276 119 887.3 0 119 277 228 887.3 0 228 278 143 887.3 0 143 279 112 887.3 0 112 280 112 887.3 0 112 281 110 887.3 0 110 282 120 887.3 0 120 283 153 887.3 0 153 284 102 887.3 0 102 285 126 887.3 0 126 286 147 887.3 0 147 287 153 887.3 0 153 288 111 887.3 0 111 289 114 887.3 0 114 290 165 887.3 0 165 291 215 887.3 0 215 292 232 887.3 0 232 293 166 887.3 0 166 294 104 887.3 0 104 295 131 887.3 0 131 296 142 887.3 0 142 297 198 887.3 0 198 298 252 887.3 0 252 299 283 887.3 0 283 300 315 887.3 0 315 301 240 887.3 0 240 Finished in 12.08 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 160,936 Reads with adapters: 65,090 (40.4%) Reads that were too short: 44 (0.0%) Reads written (passing filters): 65,046 (40.4%) Total basepairs processed: 45,489,431 bp Total written (filtered): 18,014,435 bp (39.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 65090 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 13 0.0 3 0 0 12 1 16 345 0.0 3 0 338 6 1 17 63525 0.0 3 62345 1145 31 4 18 147 0.0 3 14 130 3 19 933 0.0 3 893 18 19 3 20 57 0.0 3 35 1 0 21 21 1 0.0 3 0 0 1 22 7 0.0 3 1 0 0 6 27 1 0.0 3 1 30 1 0.0 3 1 36 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 76 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 127 5 0.0 3 0 0 0 5 133 2 0.0 3 0 0 0 2 149 5 0.0 3 0 0 0 5 151 1 0.0 3 0 0 0 1 153 1 0.0 3 1 156 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 173 1 0.0 3 0 1 176 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 217 19 0.0 3 0 1 0 18 234 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.73 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 65,046 Reads with adapters: 245 (0.4%) Reads written (passing filters): 65,046 (100.0%) Total basepairs processed: 18,014,435 bp Total written (filtered): 18,013,675 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 245 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.1% C: 46.1% G: 12.2% T: 4.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 228 1016.3 0 228 4 13 254.1 0 13 5 2 63.5 0 2 6 1 15.9 0 1 8 1 1.0 0 1