This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 171.18 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 459,742 (12.7%) Reads that were too short: 355,419 (9.8%) Reads written (passing filters): 104,323 (2.9%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 26,303,289 bp (2.4%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 459742 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 790 887.3 0 790 7 31847 887.3 0 31847 8 1354 887.3 0 1354 9 32991 887.3 0 32991 10 163 887.3 0 163 11 150 887.3 0 150 12 93 887.3 0 93 13 131 887.3 0 131 14 129 887.3 0 129 15 121 887.3 0 121 16 127 887.3 0 127 17 117 887.3 0 117 18 115 887.3 0 115 19 87 887.3 0 87 20 129 887.3 0 129 21 103 887.3 0 103 22 94 887.3 0 94 23 108 887.3 0 108 24 101 887.3 0 101 25 120 887.3 0 120 26 107 887.3 0 107 27 101 887.3 0 101 28 155 887.3 0 155 29 217 887.3 0 217 30 154 887.3 0 154 31 126 887.3 0 126 32 117 887.3 0 117 33 111 887.3 0 111 34 88 887.3 0 88 35 152 887.3 0 152 36 152 887.3 0 152 37 128 887.3 0 128 38 104 887.3 0 104 39 88 887.3 0 88 40 83 887.3 0 83 41 80 887.3 0 80 42 89 887.3 0 89 43 92 887.3 0 92 44 108 887.3 0 108 45 100 887.3 0 100 46 85 887.3 0 85 47 87 887.3 0 87 48 96 887.3 0 96 49 89 887.3 0 89 50 64 887.3 0 64 51 68 887.3 0 68 52 78 887.3 0 78 53 85 887.3 0 85 54 74 887.3 0 74 55 78 887.3 0 78 56 502 887.3 0 502 57 114 887.3 0 114 58 83 887.3 0 83 59 111 887.3 0 111 60 105 887.3 0 105 61 93 887.3 0 93 62 73 887.3 0 73 63 81 887.3 0 81 64 71 887.3 0 71 65 78 887.3 0 78 66 102 887.3 0 102 67 87 887.3 0 87 68 99 887.3 0 99 69 176 887.3 0 176 70 296 887.3 0 296 71 95 887.3 0 95 72 136 887.3 0 136 73 191 887.3 0 191 74 160 887.3 0 160 75 121 887.3 0 121 76 126 887.3 0 126 77 233 887.3 0 233 78 159 887.3 0 159 79 165 887.3 0 165 80 156 887.3 0 156 81 189 887.3 0 189 82 136 887.3 0 136 83 204 887.3 0 204 84 153 887.3 0 153 85 108 887.3 0 108 86 159 887.3 0 159 87 167 887.3 0 167 88 165 887.3 0 165 89 114 887.3 0 114 90 84 887.3 0 84 91 93 887.3 0 93 92 82 887.3 0 82 93 87 887.3 0 87 94 99 887.3 0 99 95 138 887.3 0 138 96 154 887.3 0 154 97 110 887.3 0 110 98 435 887.3 0 435 99 190 887.3 0 190 100 106 887.3 0 106 101 167 887.3 0 167 102 151 887.3 0 151 103 142 887.3 0 142 104 133 887.3 0 133 105 167 887.3 0 167 106 155 887.3 0 155 107 152 887.3 0 152 108 154 887.3 0 154 109 146 887.3 0 146 110 169 887.3 0 169 111 253 887.3 0 253 112 183 887.3 0 183 113 168 887.3 0 168 114 174 887.3 0 174 115 172 887.3 0 172 116 120 887.3 0 120 117 102 887.3 0 102 118 183 887.3 0 183 119 509 887.3 0 509 120 165 887.3 0 165 121 209 887.3 0 209 122 251 887.3 0 251 123 229 887.3 0 229 124 333 887.3 0 333 125 222 887.3 0 222 126 155 887.3 0 155 127 127 887.3 0 127 128 156 887.3 0 156 129 132 887.3 0 132 130 158 887.3 0 158 131 114 887.3 0 114 132 172 887.3 0 172 133 168 887.3 0 168 134 159 887.3 0 159 135 161 887.3 0 161 136 150 887.3 0 150 137 162 887.3 0 162 138 217 887.3 0 217 139 347 887.3 0 347 140 531 887.3 0 531 141 282 887.3 0 282 142 142 887.3 0 142 143 91 887.3 0 91 144 134 887.3 0 134 145 209 887.3 0 209 146 374 887.3 0 374 147 217 887.3 0 217 148 549 887.3 0 549 149 129 887.3 0 129 150 88 887.3 0 88 151 89 887.3 0 89 152 84 887.3 0 84 153 132 887.3 0 132 154 161 887.3 0 161 155 166 887.3 0 166 156 166 887.3 0 166 157 227 887.3 0 227 158 226 887.3 0 226 159 1861 887.3 0 1861 160 4572 887.3 0 4572 161 3181 887.3 0 3181 162 129 887.3 0 129 163 95 887.3 0 95 164 123 887.3 0 123 165 108 887.3 0 108 166 107 887.3 0 107 167 121 887.3 0 121 168 133 887.3 0 133 169 90 887.3 0 90 170 111 887.3 0 111 171 89 887.3 0 89 172 123 887.3 0 123 173 144 887.3 0 144 174 149 887.3 0 149 175 158 887.3 0 158 176 160 887.3 0 160 177 128 887.3 0 128 178 91 887.3 0 91 179 91 887.3 0 91 180 130 887.3 0 130 181 143 887.3 0 143 182 103 887.3 0 103 183 88 887.3 0 88 184 128 887.3 0 128 185 125 887.3 0 125 186 132 887.3 0 132 187 133 887.3 0 133 188 94 887.3 0 94 189 102 887.3 0 102 190 117 887.3 0 117 191 111 887.3 0 111 192 114 887.3 0 114 193 143 887.3 0 143 194 156 887.3 0 156 195 119 887.3 0 119 196 104 887.3 0 104 197 154 887.3 0 154 198 147 887.3 0 147 199 164 887.3 0 164 200 125 887.3 0 125 201 131 887.3 0 131 202 142 887.3 0 142 203 174 887.3 0 174 204 113 887.3 0 113 205 180 887.3 0 180 206 279 887.3 0 279 207 385 887.3 0 385 208 257 887.3 0 257 209 332 887.3 0 332 210 919 887.3 0 919 211 508 887.3 0 508 212 984 887.3 0 984 213 876 887.3 0 876 214 639 887.3 0 639 215 167 887.3 0 167 216 121 887.3 0 121 217 144 887.3 0 144 218 328 887.3 0 328 219 226 887.3 0 226 220 202 887.3 0 202 221 175 887.3 0 175 222 132 887.3 0 132 223 187 887.3 0 187 224 175 887.3 0 175 225 215 887.3 0 215 226 169 887.3 0 169 227 384 887.3 0 384 228 781 887.3 0 781 229 197 887.3 0 197 230 155 887.3 0 155 231 156 887.3 0 156 232 482 887.3 0 482 233 472 887.3 0 472 234 342 887.3 0 342 235 260 887.3 0 260 236 172 887.3 0 172 237 357 887.3 0 357 238 769 887.3 0 769 239 186 887.3 0 186 240 311 887.3 0 311 241 239 887.3 0 239 242 314 887.3 0 314 243 431 887.3 0 431 244 209 887.3 0 209 245 255 887.3 0 255 246 155 887.3 0 155 247 283 887.3 0 283 248 583 887.3 0 583 249 732 887.3 0 732 250 1134 887.3 0 1134 251 1618 887.3 0 1618 252 2205 887.3 0 2205 253 3890 887.3 0 3890 254 18086 887.3 0 18086 255 87967 887.3 0 87967 256 122831 887.3 0 122831 257 93276 887.3 0 93276 258 2631 887.3 0 2631 259 236 887.3 0 236 260 220 887.3 0 220 261 256 887.3 0 256 262 238 887.3 0 238 263 122 887.3 0 122 264 103 887.3 0 103 265 99 887.3 0 99 266 226 887.3 0 226 267 415 887.3 0 415 268 192 887.3 0 192 269 79 887.3 0 79 270 74 887.3 0 74 271 75 887.3 0 75 272 86 887.3 0 86 273 87 887.3 0 87 274 94 887.3 0 94 275 75 887.3 0 75 276 76 887.3 0 76 277 71 887.3 0 71 278 84 887.3 0 84 279 83 887.3 0 83 280 102 887.3 0 102 281 100 887.3 0 100 282 93 887.3 0 93 283 107 887.3 0 107 284 110 887.3 0 110 285 65 887.3 0 65 286 68 887.3 0 68 287 79 887.3 0 79 288 80 887.3 0 80 289 58 887.3 0 58 290 66 887.3 0 66 291 71 887.3 0 71 292 93 887.3 0 93 293 80 887.3 0 80 294 100 887.3 0 100 295 239 887.3 0 239 296 106 887.3 0 106 297 80 887.3 0 80 298 117 887.3 0 117 299 354 887.3 0 354 300 188 887.3 0 188 301 180 887.3 0 180 Finished in 7.85 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 104,323 Reads with adapters: 34,284 (32.9%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 34,251 (32.8%) Total basepairs processed: 26,303,289 bp Total written (filtered): 9,137,467 bp (34.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 34284 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 4 19 242 0.0 3 0 199 22 15 6 20 31571 0.0 4 30264 1058 164 67 18 21 85 0.0 4 15 64 4 1 1 22 21 0.0 4 2 4 10 3 2 23 59 0.0 4 0 0 0 45 14 25 2 0.0 4 1 0 0 0 1 26 2 0.0 4 2 28 1 0.0 4 0 0 0 1 40 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 116 2 0.0 4 0 1 0 0 1 124 2 0.0 4 0 0 0 0 2 125 3 0.0 4 0 0 0 0 3 126 37 0.0 4 0 0 1 0 36 127 2091 0.0 4 0 0 0 0 2091 128 4 0.0 4 0 0 0 0 4 130 2 0.0 4 0 0 0 0 2 132 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 135 109 0.0 4 0 0 0 0 109 140 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 0 7 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 1 1 238 2 0.0 4 0 0 0 0 2 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.95 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 34,251 Reads with adapters: 1,288 (3.8%) Reads written (passing filters): 34,251 (100.0%) Total basepairs processed: 9,137,467 bp Total written (filtered): 9,120,945 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1288 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.9% C: 10.7% G: 77.7% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 139 535.2 0 139 4 19 133.8 0 19 6 4 8.4 0 4 9 9 0.1 0 3 6 10 12 0.0 1 1 11 11 645 0.0 1 17 628 12 435 0.0 1 9 426 13 2 0.0 1 0 2 14 2 0.0 1 0 2 15 2 0.0 1 0 2 151 1 0.0 1 1 152 8 0.0 1 8 179 2 0.0 1 0 2 210 8 0.0 1 8