This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 175.19 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 263,017 (7.2%) Reads that were too short: 45,879 (1.3%) Reads written (passing filters): 217,138 (6.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 52,889,893 bp (4.8%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 263017 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5435 887.3 0 5435 7 2643 887.3 0 2643 8 80214 887.3 0 80214 9 60118 887.3 0 60118 10 122 887.3 0 122 11 50 887.3 0 50 12 49 887.3 0 49 13 40 887.3 0 40 14 48 887.3 0 48 15 48 887.3 0 48 16 44 887.3 0 44 17 64 887.3 0 64 18 65 887.3 0 65 19 47 887.3 0 47 20 47 887.3 0 47 21 83 887.3 0 83 22 82 887.3 0 82 23 94 887.3 0 94 24 43 887.3 0 43 25 44 887.3 0 44 26 174 887.3 0 174 27 92 887.3 0 92 28 64 887.3 0 64 29 85 887.3 0 85 30 59 887.3 0 59 31 59 887.3 0 59 32 54 887.3 0 54 33 72 887.3 0 72 34 88 887.3 0 88 35 57 887.3 0 57 36 54 887.3 0 54 37 72 887.3 0 72 38 81 887.3 0 81 39 32 887.3 0 32 40 61 887.3 0 61 41 59 887.3 0 59 42 53 887.3 0 53 43 55 887.3 0 55 44 45 887.3 0 45 45 51 887.3 0 51 46 35 887.3 0 35 47 68 887.3 0 68 48 51 887.3 0 51 49 44 887.3 0 44 50 70 887.3 0 70 51 77 887.3 0 77 52 51 887.3 0 51 53 60 887.3 0 60 54 62 887.3 0 62 55 58 887.3 0 58 56 39 887.3 0 39 57 47 887.3 0 47 58 43 887.3 0 43 59 51 887.3 0 51 60 46 887.3 0 46 61 63 887.3 0 63 62 42 887.3 0 42 63 46 887.3 0 46 64 55 887.3 0 55 65 84 887.3 0 84 66 71 887.3 0 71 67 59 887.3 0 59 68 88 887.3 0 88 69 135 887.3 0 135 70 168 887.3 0 168 71 161 887.3 0 161 72 56 887.3 0 56 73 56 887.3 0 56 74 60 887.3 0 60 75 80 887.3 0 80 76 144 887.3 0 144 77 105 887.3 0 105 78 70 887.3 0 70 79 163 887.3 0 163 80 218 887.3 0 218 81 238 887.3 0 238 82 99 887.3 0 99 83 45 887.3 0 45 84 59 887.3 0 59 85 59 887.3 0 59 86 59 887.3 0 59 87 49 887.3 0 49 88 62 887.3 0 62 89 136 887.3 0 136 90 61 887.3 0 61 91 71 887.3 0 71 92 66 887.3 0 66 93 62 887.3 0 62 94 63 887.3 0 63 95 82 887.3 0 82 96 83 887.3 0 83 97 82 887.3 0 82 98 67 887.3 0 67 99 93 887.3 0 93 100 111 887.3 0 111 101 137 887.3 0 137 102 54 887.3 0 54 103 61 887.3 0 61 104 61 887.3 0 61 105 53 887.3 0 53 106 45 887.3 0 45 107 73 887.3 0 73 108 57 887.3 0 57 109 52 887.3 0 52 110 69 887.3 0 69 111 65 887.3 0 65 112 132 887.3 0 132 113 183 887.3 0 183 114 146 887.3 0 146 115 79 887.3 0 79 116 84 887.3 0 84 117 684 887.3 0 684 118 296 887.3 0 296 119 172 887.3 0 172 120 82 887.3 0 82 121 59 887.3 0 59 122 72 887.3 0 72 123 77 887.3 0 77 124 56 887.3 0 56 125 76 887.3 0 76 126 90 887.3 0 90 127 96 887.3 0 96 128 99 887.3 0 99 129 104 887.3 0 104 130 119 887.3 0 119 131 401 887.3 0 401 132 335 887.3 0 335 133 211 887.3 0 211 134 137 887.3 0 137 135 122 887.3 0 122 136 211 887.3 0 211 137 438 887.3 0 438 138 217 887.3 0 217 139 191 887.3 0 191 140 130 887.3 0 130 141 66 887.3 0 66 142 69 887.3 0 69 143 91 887.3 0 91 144 93 887.3 0 93 145 101 887.3 0 101 146 116 887.3 0 116 147 101 887.3 0 101 148 85 887.3 0 85 149 60 887.3 0 60 150 72 887.3 0 72 151 66 887.3 0 66 152 71 887.3 0 71 153 93 887.3 0 93 154 132 887.3 0 132 155 105 887.3 0 105 156 644 887.3 0 644 157 158 887.3 0 158 158 141 887.3 0 141 159 91 887.3 0 91 160 84 887.3 0 84 161 101 887.3 0 101 162 84 887.3 0 84 163 95 887.3 0 95 164 122 887.3 0 122 165 211 887.3 0 211 166 104 887.3 0 104 167 132 887.3 0 132 168 214 887.3 0 214 169 163 887.3 0 163 170 159 887.3 0 159 171 220 887.3 0 220 172 181 887.3 0 181 173 225 887.3 0 225 174 270 887.3 0 270 175 525 887.3 0 525 176 873 887.3 0 873 177 1239 887.3 0 1239 178 8563 887.3 0 8563 179 5140 887.3 0 5140 180 15641 887.3 0 15641 181 4555 887.3 0 4555 182 6189 887.3 0 6189 183 1863 887.3 0 1863 184 280 887.3 0 280 185 150 887.3 0 150 186 74 887.3 0 74 187 62 887.3 0 62 188 185 887.3 0 185 189 131 887.3 0 131 190 92 887.3 0 92 191 109 887.3 0 109 192 115 887.3 0 115 193 117 887.3 0 117 194 209 887.3 0 209 195 375 887.3 0 375 196 1292 887.3 0 1292 197 1514 887.3 0 1514 198 917 887.3 0 917 199 321 887.3 0 321 200 233 887.3 0 233 201 708 887.3 0 708 202 1125 887.3 0 1125 203 920 887.3 0 920 204 575 887.3 0 575 205 62 887.3 0 62 206 66 887.3 0 66 207 72 887.3 0 72 208 65 887.3 0 65 209 77 887.3 0 77 210 78 887.3 0 78 211 70 887.3 0 70 212 80 887.3 0 80 213 75 887.3 0 75 214 71 887.3 0 71 215 138 887.3 0 138 216 197 887.3 0 197 217 243 887.3 0 243 218 117 887.3 0 117 219 97 887.3 0 97 220 99 887.3 0 99 221 176 887.3 0 176 222 595 887.3 0 595 223 799 887.3 0 799 224 464 887.3 0 464 225 366 887.3 0 366 226 73 887.3 0 73 227 113 887.3 0 113 228 86 887.3 0 86 229 67 887.3 0 67 230 73 887.3 0 73 231 83 887.3 0 83 232 75 887.3 0 75 233 107 887.3 0 107 234 385 887.3 0 385 235 350 887.3 0 350 236 574 887.3 0 574 237 212 887.3 0 212 238 247 887.3 0 247 239 1543 887.3 0 1543 240 643 887.3 0 643 241 799 887.3 0 799 242 546 887.3 0 546 243 687 887.3 0 687 244 369 887.3 0 369 245 162 887.3 0 162 246 96 887.3 0 96 247 96 887.3 0 96 248 82 887.3 0 82 249 58 887.3 0 58 250 97 887.3 0 97 251 128 887.3 0 128 252 172 887.3 0 172 253 188 887.3 0 188 254 248 887.3 0 248 255 120 887.3 0 120 256 317 887.3 0 317 257 488 887.3 0 488 258 565 887.3 0 565 259 1324 887.3 0 1324 260 1143 887.3 0 1143 261 878 887.3 0 878 262 250 887.3 0 250 263 329 887.3 0 329 264 2199 887.3 0 2199 265 4725 887.3 0 4725 266 4407 887.3 0 4407 267 1349 887.3 0 1349 268 57 887.3 0 57 269 84 887.3 0 84 270 92 887.3 0 92 271 89 887.3 0 89 272 88 887.3 0 88 273 118 887.3 0 118 274 184 887.3 0 184 275 141 887.3 0 141 276 297 887.3 0 297 277 229 887.3 0 229 278 361 887.3 0 361 279 164 887.3 0 164 280 69 887.3 0 69 281 51 887.3 0 51 282 69 887.3 0 69 283 74 887.3 0 74 284 95 887.3 0 95 285 81 887.3 0 81 286 108 887.3 0 108 287 114 887.3 0 114 288 118 887.3 0 118 289 142 887.3 0 142 290 260 887.3 0 260 291 3593 887.3 0 3593 292 2391 887.3 0 2391 293 2048 887.3 0 2048 294 338 887.3 0 338 295 231 887.3 0 231 296 136 887.3 0 136 297 174 887.3 0 174 298 171 887.3 0 171 299 272 887.3 0 272 300 196 887.3 0 196 301 164 887.3 0 164 Finished in 16.00 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 217,138 Reads with adapters: 64,783 (29.8%) Reads that were too short: 68 (0.0%) Reads written (passing filters): 64,715 (29.8%) Total basepairs processed: 52,889,893 bp Total written (filtered): 17,527,900 bp (33.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 64783 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 22 0.0 3 0 0 18 2 2 19 567 0.0 3 0 495 39 23 10 20 63074 0.0 4 60231 2352 352 108 31 21 162 0.0 4 28 121 8 2 3 22 20 0.0 4 1 2 10 5 2 23 111 0.0 4 2 0 0 78 31 24 3 0.0 4 0 0 0 0 3 25 1 0.0 4 0 0 0 0 1 27 1 0.0 4 0 0 0 0 1 29 2 0.0 4 0 0 0 0 2 34 3 0.0 4 0 0 0 0 3 36 1 0.0 4 1 39 6 0.0 4 0 0 0 0 6 40 6 0.0 4 1 0 0 1 4 45 1 0.0 4 0 1 54 3 0.0 4 0 0 0 0 3 66 1 0.0 4 0 0 0 0 1 76 1 0.0 4 1 79 2 0.0 4 0 0 0 0 2 85 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 98 2 0.0 4 0 0 0 0 2 101 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 8 0.0 4 0 0 0 0 8 127 633 0.0 4 0 0 0 0 633 128 4 0.0 4 0 0 0 0 4 129 1 0.0 4 0 0 0 0 1 130 86 0.0 4 0 0 0 0 86 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 136 3 0.0 4 0 0 0 0 3 137 2 0.0 4 0 0 0 0 2 140 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 209 2 0.0 4 0 0 0 0 2 213 2 0.0 4 0 0 0 0 2 227 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 269 1 0.0 4 1 275 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.01 s (62 us/read; 0.97 M reads/minute). === Summary === Total reads processed: 64,715 Reads with adapters: 15,094 (23.3%) Reads written (passing filters): 64,715 (100.0%) Total basepairs processed: 17,527,900 bp Total written (filtered): 17,377,315 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 15094 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 6.1% G: 91.2% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 44 1011.2 0 44 4 17 252.8 0 17 5 1 63.2 0 1 6 3 15.8 0 3 7 2 3.9 0 2 8 6 1.0 0 6 9 4933 0.2 0 223 4710 10 8638 0.1 1 515 8123 11 1344 0.0 1 36 1308 12 51 0.0 1 0 51 13 30 0.0 1 0 30 14 1 0.0 1 0 1 15 1 0.0 1 0 1 150 18 0.0 1 12 6 177 1 0.0 1 0 1 189 1 0.0 1 1 208 2 0.0 1 2 226 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.