This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 175.17 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 243,586 (6.7%) Reads that were too short: 33,001 (0.9%) Reads written (passing filters): 210,585 (5.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 52,457,838 bp (4.8%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 243586 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5344 887.3 0 5344 7 1951 887.3 0 1951 8 67051 887.3 0 67051 9 78729 887.3 0 78729 10 119 887.3 0 119 11 30 887.3 0 30 12 31 887.3 0 31 13 24 887.3 0 24 14 31 887.3 0 31 15 26 887.3 0 26 16 37 887.3 0 37 17 42 887.3 0 42 18 28 887.3 0 28 19 30 887.3 0 30 20 20 887.3 0 20 21 28 887.3 0 28 22 36 887.3 0 36 23 35 887.3 0 35 24 45 887.3 0 45 25 34 887.3 0 34 26 44 887.3 0 44 27 30 887.3 0 30 28 27 887.3 0 27 29 60 887.3 0 60 30 49 887.3 0 49 31 45 887.3 0 45 32 45 887.3 0 45 33 50 887.3 0 50 34 45 887.3 0 45 35 32 887.3 0 32 36 30 887.3 0 30 37 46 887.3 0 46 38 58 887.3 0 58 39 28 887.3 0 28 40 42 887.3 0 42 41 50 887.3 0 50 42 36 887.3 0 36 43 42 887.3 0 42 44 29 887.3 0 29 45 31 887.3 0 31 46 37 887.3 0 37 47 44 887.3 0 44 48 38 887.3 0 38 49 30 887.3 0 30 50 70 887.3 0 70 51 38 887.3 0 38 52 31 887.3 0 31 53 35 887.3 0 35 54 37 887.3 0 37 55 40 887.3 0 40 56 33 887.3 0 33 57 19 887.3 0 19 58 25 887.3 0 25 59 35 887.3 0 35 60 44 887.3 0 44 61 48 887.3 0 48 62 28 887.3 0 28 63 28 887.3 0 28 64 52 887.3 0 52 65 53 887.3 0 53 66 57 887.3 0 57 67 33 887.3 0 33 68 52 887.3 0 52 69 151 887.3 0 151 70 165 887.3 0 165 71 164 887.3 0 164 72 44 887.3 0 44 73 30 887.3 0 30 74 46 887.3 0 46 75 34 887.3 0 34 76 38 887.3 0 38 77 40 887.3 0 40 78 32 887.3 0 32 79 146 887.3 0 146 80 132 887.3 0 132 81 204 887.3 0 204 82 27 887.3 0 27 83 35 887.3 0 35 84 29 887.3 0 29 85 34 887.3 0 34 86 28 887.3 0 28 87 27 887.3 0 27 88 40 887.3 0 40 89 30 887.3 0 30 90 37 887.3 0 37 91 38 887.3 0 38 92 35 887.3 0 35 93 33 887.3 0 33 94 48 887.3 0 48 95 40 887.3 0 40 96 49 887.3 0 49 97 28 887.3 0 28 98 44 887.3 0 44 99 57 887.3 0 57 100 70 887.3 0 70 101 57 887.3 0 57 102 44 887.3 0 44 103 39 887.3 0 39 104 32 887.3 0 32 105 26 887.3 0 26 106 33 887.3 0 33 107 38 887.3 0 38 108 37 887.3 0 37 109 29 887.3 0 29 110 43 887.3 0 43 111 65 887.3 0 65 112 66 887.3 0 66 113 100 887.3 0 100 114 65 887.3 0 65 115 46 887.3 0 46 116 37 887.3 0 37 117 219 887.3 0 219 118 115 887.3 0 115 119 112 887.3 0 112 120 50 887.3 0 50 121 34 887.3 0 34 122 38 887.3 0 38 123 48 887.3 0 48 124 39 887.3 0 39 125 41 887.3 0 41 126 62 887.3 0 62 127 62 887.3 0 62 128 65 887.3 0 65 129 88 887.3 0 88 130 70 887.3 0 70 131 137 887.3 0 137 132 118 887.3 0 118 133 106 887.3 0 106 134 70 887.3 0 70 135 103 887.3 0 103 136 144 887.3 0 144 137 404 887.3 0 404 138 197 887.3 0 197 139 72 887.3 0 72 140 68 887.3 0 68 141 41 887.3 0 41 142 43 887.3 0 43 143 52 887.3 0 52 144 64 887.3 0 64 145 60 887.3 0 60 146 66 887.3 0 66 147 66 887.3 0 66 148 49 887.3 0 49 149 33 887.3 0 33 150 35 887.3 0 35 151 52 887.3 0 52 152 52 887.3 0 52 153 68 887.3 0 68 154 94 887.3 0 94 155 72 887.3 0 72 156 603 887.3 0 603 157 118 887.3 0 118 158 77 887.3 0 77 159 51 887.3 0 51 160 68 887.3 0 68 161 60 887.3 0 60 162 76 887.3 0 76 163 70 887.3 0 70 164 100 887.3 0 100 165 218 887.3 0 218 166 84 887.3 0 84 167 149 887.3 0 149 168 155 887.3 0 155 169 123 887.3 0 123 170 118 887.3 0 118 171 194 887.3 0 194 172 161 887.3 0 161 173 185 887.3 0 185 174 204 887.3 0 204 175 484 887.3 0 484 176 673 887.3 0 673 177 1026 887.3 0 1026 178 8387 887.3 0 8387 179 4903 887.3 0 4903 180 15345 887.3 0 15345 181 4117 887.3 0 4117 182 6060 887.3 0 6060 183 1312 887.3 0 1312 184 156 887.3 0 156 185 103 887.3 0 103 186 51 887.3 0 51 187 57 887.3 0 57 188 157 887.3 0 157 189 84 887.3 0 84 190 52 887.3 0 52 191 77 887.3 0 77 192 121 887.3 0 121 193 79 887.3 0 79 194 148 887.3 0 148 195 188 887.3 0 188 196 698 887.3 0 698 197 878 887.3 0 878 198 581 887.3 0 581 199 148 887.3 0 148 200 166 887.3 0 166 201 302 887.3 0 302 202 612 887.3 0 612 203 351 887.3 0 351 204 221 887.3 0 221 205 29 887.3 0 29 206 44 887.3 0 44 207 49 887.3 0 49 208 38 887.3 0 38 209 48 887.3 0 48 210 40 887.3 0 40 211 47 887.3 0 47 212 40 887.3 0 40 213 55 887.3 0 55 214 55 887.3 0 55 215 100 887.3 0 100 216 127 887.3 0 127 217 198 887.3 0 198 218 52 887.3 0 52 219 60 887.3 0 60 220 55 887.3 0 55 221 89 887.3 0 89 222 254 887.3 0 254 223 428 887.3 0 428 224 305 887.3 0 305 225 271 887.3 0 271 226 57 887.3 0 57 227 83 887.3 0 83 228 56 887.3 0 56 229 53 887.3 0 53 230 49 887.3 0 49 231 67 887.3 0 67 232 82 887.3 0 82 233 58 887.3 0 58 234 399 887.3 0 399 235 319 887.3 0 319 236 572 887.3 0 572 237 180 887.3 0 180 238 205 887.3 0 205 239 1264 887.3 0 1264 240 428 887.3 0 428 241 571 887.3 0 571 242 310 887.3 0 310 243 422 887.3 0 422 244 162 887.3 0 162 245 80 887.3 0 80 246 83 887.3 0 83 247 49 887.3 0 49 248 59 887.3 0 59 249 48 887.3 0 48 250 91 887.3 0 91 251 92 887.3 0 92 252 111 887.3 0 111 253 167 887.3 0 167 254 174 887.3 0 174 255 78 887.3 0 78 256 225 887.3 0 225 257 239 887.3 0 239 258 291 887.3 0 291 259 888 887.3 0 888 260 942 887.3 0 942 261 567 887.3 0 567 262 176 887.3 0 176 263 232 887.3 0 232 264 1563 887.3 0 1563 265 4106 887.3 0 4106 266 3468 887.3 0 3468 267 1154 887.3 0 1154 268 47 887.3 0 47 269 57 887.3 0 57 270 49 887.3 0 49 271 62 887.3 0 62 272 50 887.3 0 50 273 66 887.3 0 66 274 85 887.3 0 85 275 66 887.3 0 66 276 139 887.3 0 139 277 99 887.3 0 99 278 132 887.3 0 132 279 69 887.3 0 69 280 56 887.3 0 56 281 31 887.3 0 31 282 60 887.3 0 60 283 49 887.3 0 49 284 60 887.3 0 60 285 67 887.3 0 67 286 57 887.3 0 57 287 68 887.3 0 68 288 81 887.3 0 81 289 87 887.3 0 87 290 167 887.3 0 167 291 2703 887.3 0 2703 292 1657 887.3 0 1657 293 1745 887.3 0 1745 294 349 887.3 0 349 295 230 887.3 0 230 296 109 887.3 0 109 297 177 887.3 0 177 298 90 887.3 0 90 299 203 887.3 0 203 300 135 887.3 0 135 301 111 887.3 0 111 Finished in 14.66 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 210,585 Reads with adapters: 70,081 (33.3%) Reads that were too short: 62 (0.0%) Reads written (passing filters): 70,019 (33.2%) Total basepairs processed: 52,457,838 bp Total written (filtered): 19,316,486 bp (36.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 70081 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 16 0.0 3 0 0 16 16 403 0.0 3 0 393 7 3 17 64846 0.0 3 63353 1432 50 11 18 151 0.0 3 19 128 2 2 19 72 0.0 3 14 24 28 6 20 4464 0.0 3 4348 92 3 21 21 24 0.0 3 5 17 1 1 22 4 0.0 3 1 1 1 1 23 2 0.0 3 0 0 0 2 24 1 0.0 3 1 26 1 0.0 3 1 27 1 0.0 3 0 0 0 1 28 1 0.0 3 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 35 7 0.0 3 0 0 0 7 38 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 60 3 0.0 3 0 0 0 3 61 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 0 3 79 1 0.0 3 0 0 0 1 80 4 0.0 3 0 0 0 4 83 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 91 4 0.0 3 0 0 0 4 102 18 0.0 3 0 0 0 18 103 7 0.0 3 0 0 0 7 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 140 3 0.0 3 0 0 0 3 151 1 0.0 3 0 0 0 1 159 4 0.0 3 0 0 0 4 163 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 279 3 0.0 3 0 0 0 3 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.09 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 70,019 Reads with adapters: 306 (0.4%) Reads written (passing filters): 70,019 (100.0%) Total basepairs processed: 19,316,486 bp Total written (filtered): 19,315,516 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 306 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 73.9% C: 5.9% G: 16.7% T: 3.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 260 1094.0 0 260 4 40 273.5 0 40 5 6 68.4 0 6