This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.73 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 272,640 (7.5%) Reads that were too short: 216,670 (6.0%) Reads written (passing filters): 55,970 (1.5%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 12,647,733 bp (1.2%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 272640 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3326 887.3 0 3326 7 2516 887.3 0 2516 8 2187 887.3 0 2187 9 33 887.3 0 33 10 45 887.3 0 45 11 29 887.3 0 29 12 32 887.3 0 32 13 33 887.3 0 33 14 29 887.3 0 29 15 34 887.3 0 34 16 35 887.3 0 35 17 35 887.3 0 35 18 19 887.3 0 19 19 30 887.3 0 30 20 44 887.3 0 44 21 40 887.3 0 40 22 22 887.3 0 22 23 24 887.3 0 24 24 32 887.3 0 32 25 24 887.3 0 24 26 17 887.3 0 17 27 18 887.3 0 18 28 25 887.3 0 25 29 27 887.3 0 27 30 20 887.3 0 20 31 12 887.3 0 12 32 19 887.3 0 19 33 16 887.3 0 16 34 15 887.3 0 15 35 34 887.3 0 34 36 23 887.3 0 23 37 26 887.3 0 26 38 25 887.3 0 25 39 17 887.3 0 17 40 24 887.3 0 24 41 19 887.3 0 19 42 48 887.3 0 48 43 29 887.3 0 29 44 32 887.3 0 32 45 20 887.3 0 20 46 23 887.3 0 23 47 22 887.3 0 22 48 25 887.3 0 25 49 34 887.3 0 34 50 56 887.3 0 56 51 71 887.3 0 71 52 46 887.3 0 46 53 33 887.3 0 33 54 51 887.3 0 51 55 66 887.3 0 66 56 72 887.3 0 72 57 39 887.3 0 39 58 57 887.3 0 57 59 54 887.3 0 54 60 46 887.3 0 46 61 52 887.3 0 52 62 150 887.3 0 150 63 65 887.3 0 65 64 74 887.3 0 74 65 120 887.3 0 120 66 68 887.3 0 68 67 61 887.3 0 61 68 131 887.3 0 131 69 140 887.3 0 140 70 167 887.3 0 167 71 150 887.3 0 150 72 249 887.3 0 249 73 311 887.3 0 311 74 593 887.3 0 593 75 18163 887.3 0 18163 76 10269 887.3 0 10269 77 8196 887.3 0 8196 78 69 887.3 0 69 79 27 887.3 0 27 80 30 887.3 0 30 81 32 887.3 0 32 82 29 887.3 0 29 83 21 887.3 0 21 84 25 887.3 0 25 85 18 887.3 0 18 86 20 887.3 0 20 87 30 887.3 0 30 88 40 887.3 0 40 89 23 887.3 0 23 90 24 887.3 0 24 91 58 887.3 0 58 92 26 887.3 0 26 93 17 887.3 0 17 94 29 887.3 0 29 95 66 887.3 0 66 96 44 887.3 0 44 97 29 887.3 0 29 98 53 887.3 0 53 99 34 887.3 0 34 100 17 887.3 0 17 101 29 887.3 0 29 102 23 887.3 0 23 103 30 887.3 0 30 104 22 887.3 0 22 105 43 887.3 0 43 106 31 887.3 0 31 107 39 887.3 0 39 108 31 887.3 0 31 109 34 887.3 0 34 110 25 887.3 0 25 111 20 887.3 0 20 112 16 887.3 0 16 113 22 887.3 0 22 114 17 887.3 0 17 115 43 887.3 0 43 116 23 887.3 0 23 117 31 887.3 0 31 118 27 887.3 0 27 119 45 887.3 0 45 120 42 887.3 0 42 121 69 887.3 0 69 122 30 887.3 0 30 123 41 887.3 0 41 124 34 887.3 0 34 125 92 887.3 0 92 126 171 887.3 0 171 127 159 887.3 0 159 128 56 887.3 0 56 129 40 887.3 0 40 130 27 887.3 0 27 131 28 887.3 0 28 132 31 887.3 0 31 133 26 887.3 0 26 134 59 887.3 0 59 135 70 887.3 0 70 136 60 887.3 0 60 137 61 887.3 0 61 138 43 887.3 0 43 139 104 887.3 0 104 140 51 887.3 0 51 141 25 887.3 0 25 142 43 887.3 0 43 143 133 887.3 0 133 144 71 887.3 0 71 145 44 887.3 0 44 146 40 887.3 0 40 147 31 887.3 0 31 148 22 887.3 0 22 149 25 887.3 0 25 150 46 887.3 0 46 151 26 887.3 0 26 152 25 887.3 0 25 153 30 887.3 0 30 154 36 887.3 0 36 155 28 887.3 0 28 156 34 887.3 0 34 157 38 887.3 0 38 158 48 887.3 0 48 159 27 887.3 0 27 160 27 887.3 0 27 161 48 887.3 0 48 162 32 887.3 0 32 163 37 887.3 0 37 164 54 887.3 0 54 165 56 887.3 0 56 166 66 887.3 0 66 167 69 887.3 0 69 168 93 887.3 0 93 169 56 887.3 0 56 170 29 887.3 0 29 171 29 887.3 0 29 172 39 887.3 0 39 173 37 887.3 0 37 174 34 887.3 0 34 175 32 887.3 0 32 176 26 887.3 0 26 177 34 887.3 0 34 178 44 887.3 0 44 179 40 887.3 0 40 180 66 887.3 0 66 181 95 887.3 0 95 182 80 887.3 0 80 183 79 887.3 0 79 184 46 887.3 0 46 185 51 887.3 0 51 186 94 887.3 0 94 187 130 887.3 0 130 188 126 887.3 0 126 189 87 887.3 0 87 190 56 887.3 0 56 191 72 887.3 0 72 192 75 887.3 0 75 193 178 887.3 0 178 194 174 887.3 0 174 195 96 887.3 0 96 196 77 887.3 0 77 197 591 887.3 0 591 198 485 887.3 0 485 199 219 887.3 0 219 200 46 887.3 0 46 201 98 887.3 0 98 202 55 887.3 0 55 203 75 887.3 0 75 204 95 887.3 0 95 205 157 887.3 0 157 206 80 887.3 0 80 207 57 887.3 0 57 208 70 887.3 0 70 209 40 887.3 0 40 210 64 887.3 0 64 211 166 887.3 0 166 212 68 887.3 0 68 213 74 887.3 0 74 214 77 887.3 0 77 215 78 887.3 0 78 216 81 887.3 0 81 217 245 887.3 0 245 218 327 887.3 0 327 219 268 887.3 0 268 220 177 887.3 0 177 221 201 887.3 0 201 222 80 887.3 0 80 223 420 887.3 0 420 224 467 887.3 0 467 225 523 887.3 0 523 226 176 887.3 0 176 227 182 887.3 0 182 228 210 887.3 0 210 229 175 887.3 0 175 230 316 887.3 0 316 231 159 887.3 0 159 232 212 887.3 0 212 233 326 887.3 0 326 234 431 887.3 0 431 235 820 887.3 0 820 236 1148 887.3 0 1148 237 1161 887.3 0 1161 238 17041 887.3 0 17041 239 32255 887.3 0 32255 240 25711 887.3 0 25711 241 397 887.3 0 397 242 402 887.3 0 402 243 426 887.3 0 426 244 418 887.3 0 418 245 611 887.3 0 611 246 831 887.3 0 831 247 1448 887.3 0 1448 248 21431 887.3 0 21431 249 41383 887.3 0 41383 250 24783 887.3 0 24783 251 17953 887.3 0 17953 252 103 887.3 0 103 253 42 887.3 0 42 254 28 887.3 0 28 255 39 887.3 0 39 256 35 887.3 0 35 257 53 887.3 0 53 258 61 887.3 0 61 259 281 887.3 0 281 260 103 887.3 0 103 261 104 887.3 0 104 262 239 887.3 0 239 263 99 887.3 0 99 264 55 887.3 0 55 265 31 887.3 0 31 266 36 887.3 0 36 267 39 887.3 0 39 268 62 887.3 0 62 269 119 887.3 0 119 270 74 887.3 0 74 271 112 887.3 0 112 272 106 887.3 0 106 273 80 887.3 0 80 274 50 887.3 0 50 275 66 887.3 0 66 276 67 887.3 0 67 277 74 887.3 0 74 278 146 887.3 0 146 279 273 887.3 0 273 280 327 887.3 0 327 281 374 887.3 0 374 282 5001 887.3 0 5001 283 3796 887.3 0 3796 284 4863 887.3 0 4863 285 202 887.3 0 202 286 167 887.3 0 167 287 120 887.3 0 120 288 83 887.3 0 83 289 58 887.3 0 58 290 151 887.3 0 151 291 85 887.3 0 85 292 107 887.3 0 107 293 87 887.3 0 87 294 561 887.3 0 561 295 1391 887.3 0 1391 296 942 887.3 0 942 297 297 887.3 0 297 298 248 887.3 0 248 299 246 887.3 0 246 300 343 887.3 0 343 301 293 887.3 0 293 Finished in 3.50 s (63 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 55,970 Reads with adapters: 2,870 (5.1%) Reads that were too short: 88 (0.2%) Reads written (passing filters): 2,782 (5.0%) Total basepairs processed: 12,647,733 bp Total written (filtered): 701,468 bp (5.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2870 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 12 0.0 3 0 12 17 2310 0.0 3 2258 51 1 18 4 0.0 3 1 3 19 2 0.0 3 0 0 2 26 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 1 48 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 1 78 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 14 0.0 3 0 0 0 14 92 3 0.0 3 0 0 0 3 93 4 0.0 3 0 0 0 4 94 2 0.0 3 0 0 0 2 95 2 0.0 3 0 0 0 2 96 34 0.0 3 0 0 0 34 98 169 0.0 3 0 0 1 168 99 47 0.0 3 0 0 0 47 101 29 0.0 3 0 0 1 28 102 7 0.0 3 0 0 1 6 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 9 0.0 3 0 0 0 9 116 61 0.0 3 0 0 0 61 117 32 0.0 3 0 0 0 32 118 1 0.0 3 0 0 0 1 119 6 0.0 3 0 0 0 6 120 6 0.0 3 0 0 0 6 122 2 0.0 3 0 0 0 2 124 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 176 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 3 0.0 3 0 0 0 3 185 2 0.0 3 0 0 0 2 199 2 0.0 3 0 0 0 2 203 17 0.0 3 0 0 0 17 204 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 8 0.0 3 0 0 1 7 207 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 2 0.0 3 0 0 0 2 219 17 0.0 3 0 0 0 17 220 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 228 3 0.0 3 0 0 0 3 256 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 2,782 Reads with adapters: 5 (0.2%) Reads written (passing filters): 2,782 (100.0%) Total basepairs processed: 701,468 bp Total written (filtered): 701,449 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.0% C: 40.0% G: 0.0% T: 20.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 3 43.5 0 3 4 1 10.9 0 1 6 1 0.7 0 1