This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 177.62 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 311,670 (8.6%) Reads that were too short: 22,537 (0.6%) Reads written (passing filters): 289,133 (8.0%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 77,951,724 bp (7.1%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 311670 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1842 887.3 0 1842 7 11241 887.3 0 11241 8 116853 887.3 0 116853 9 110329 887.3 0 110329 10 184 887.3 0 184 11 51 887.3 0 51 12 51 887.3 0 51 13 38 887.3 0 38 14 68 887.3 0 68 15 35 887.3 0 35 16 42 887.3 0 42 17 49 887.3 0 49 18 54 887.3 0 54 19 42 887.3 0 42 20 64 887.3 0 64 21 60 887.3 0 60 22 42 887.3 0 42 23 48 887.3 0 48 24 58 887.3 0 58 25 47 887.3 0 47 26 37 887.3 0 37 27 53 887.3 0 53 28 58 887.3 0 58 29 81 887.3 0 81 30 40 887.3 0 40 31 30 887.3 0 30 32 37 887.3 0 37 33 37 887.3 0 37 34 80 887.3 0 80 35 57 887.3 0 57 36 66 887.3 0 66 37 75 887.3 0 75 38 57 887.3 0 57 39 67 887.3 0 67 40 52 887.3 0 52 41 61 887.3 0 61 42 63 887.3 0 63 43 39 887.3 0 39 44 40 887.3 0 40 45 40 887.3 0 40 46 70 887.3 0 70 47 38 887.3 0 38 48 44 887.3 0 44 49 60 887.3 0 60 50 51 887.3 0 51 51 48 887.3 0 48 52 53 887.3 0 53 53 87 887.3 0 87 54 115 887.3 0 115 55 108 887.3 0 108 56 65 887.3 0 65 57 53 887.3 0 53 58 98 887.3 0 98 59 79 887.3 0 79 60 68 887.3 0 68 61 56 887.3 0 56 62 64 887.3 0 64 63 128 887.3 0 128 64 159 887.3 0 159 65 126 887.3 0 126 66 63 887.3 0 63 67 99 887.3 0 99 68 126 887.3 0 126 69 116 887.3 0 116 70 72 887.3 0 72 71 117 887.3 0 117 72 587 887.3 0 587 73 1487 887.3 0 1487 74 1624 887.3 0 1624 75 793 887.3 0 793 76 46 887.3 0 46 77 51 887.3 0 51 78 55 887.3 0 55 79 65 887.3 0 65 80 51 887.3 0 51 81 42 887.3 0 42 82 60 887.3 0 60 83 40 887.3 0 40 84 59 887.3 0 59 85 90 887.3 0 90 86 126 887.3 0 126 87 125 887.3 0 125 88 249 887.3 0 249 89 103 887.3 0 103 90 69 887.3 0 69 91 80 887.3 0 80 92 70 887.3 0 70 93 70 887.3 0 70 94 71 887.3 0 71 95 76 887.3 0 76 96 65 887.3 0 65 97 63 887.3 0 63 98 64 887.3 0 64 99 58 887.3 0 58 100 82 887.3 0 82 101 74 887.3 0 74 102 65 887.3 0 65 103 45 887.3 0 45 104 64 887.3 0 64 105 107 887.3 0 107 106 67 887.3 0 67 107 99 887.3 0 99 108 118 887.3 0 118 109 108 887.3 0 108 110 107 887.3 0 107 111 75 887.3 0 75 112 50 887.3 0 50 113 46 887.3 0 46 114 60 887.3 0 60 115 103 887.3 0 103 116 64 887.3 0 64 117 48 887.3 0 48 118 63 887.3 0 63 119 64 887.3 0 64 120 38 887.3 0 38 121 77 887.3 0 77 122 71 887.3 0 71 123 55 887.3 0 55 124 49 887.3 0 49 125 53 887.3 0 53 126 69 887.3 0 69 127 70 887.3 0 70 128 97 887.3 0 97 129 106 887.3 0 106 130 118 887.3 0 118 131 90 887.3 0 90 132 67 887.3 0 67 133 78 887.3 0 78 134 66 887.3 0 66 135 136 887.3 0 136 136 173 887.3 0 173 137 154 887.3 0 154 138 71 887.3 0 71 139 59 887.3 0 59 140 59 887.3 0 59 141 123 887.3 0 123 142 263 887.3 0 263 143 118 887.3 0 118 144 218 887.3 0 218 145 248 887.3 0 248 146 2078 887.3 0 2078 147 1519 887.3 0 1519 148 829 887.3 0 829 149 235 887.3 0 235 150 184 887.3 0 184 151 121 887.3 0 121 152 68 887.3 0 68 153 107 887.3 0 107 154 122 887.3 0 122 155 289 887.3 0 289 156 290 887.3 0 290 157 289 887.3 0 289 158 1275 887.3 0 1275 159 904 887.3 0 904 160 902 887.3 0 902 161 256 887.3 0 256 162 338 887.3 0 338 163 462 887.3 0 462 164 89 887.3 0 89 165 134 887.3 0 134 166 173 887.3 0 173 167 233 887.3 0 233 168 138 887.3 0 138 169 124 887.3 0 124 170 233 887.3 0 233 171 250 887.3 0 250 172 215 887.3 0 215 173 288 887.3 0 288 174 3355 887.3 0 3355 175 4388 887.3 0 4388 176 3020 887.3 0 3020 177 174 887.3 0 174 178 124 887.3 0 124 179 169 887.3 0 169 180 1010 887.3 0 1010 181 2262 887.3 0 2262 182 1339 887.3 0 1339 183 122 887.3 0 122 184 162 887.3 0 162 185 254 887.3 0 254 186 370 887.3 0 370 187 1937 887.3 0 1937 188 1403 887.3 0 1403 189 895 887.3 0 895 190 92 887.3 0 92 191 78 887.3 0 78 192 74 887.3 0 74 193 68 887.3 0 68 194 95 887.3 0 95 195 85 887.3 0 85 196 80 887.3 0 80 197 90 887.3 0 90 198 65 887.3 0 65 199 66 887.3 0 66 200 106 887.3 0 106 201 100 887.3 0 100 202 84 887.3 0 84 203 140 887.3 0 140 204 223 887.3 0 223 205 229 887.3 0 229 206 138 887.3 0 138 207 84 887.3 0 84 208 87 887.3 0 87 209 95 887.3 0 95 210 62 887.3 0 62 211 62 887.3 0 62 212 68 887.3 0 68 213 80 887.3 0 80 214 83 887.3 0 83 215 116 887.3 0 116 216 132 887.3 0 132 217 92 887.3 0 92 218 98 887.3 0 98 219 113 887.3 0 113 220 116 887.3 0 116 221 82 887.3 0 82 222 110 887.3 0 110 223 114 887.3 0 114 224 90 887.3 0 90 225 95 887.3 0 95 226 85 887.3 0 85 227 164 887.3 0 164 228 180 887.3 0 180 229 292 887.3 0 292 230 86 887.3 0 86 231 137 887.3 0 137 232 160 887.3 0 160 233 113 887.3 0 113 234 119 887.3 0 119 235 104 887.3 0 104 236 135 887.3 0 135 237 168 887.3 0 168 238 243 887.3 0 243 239 178 887.3 0 178 240 126 887.3 0 126 241 102 887.3 0 102 242 109 887.3 0 109 243 100 887.3 0 100 244 112 887.3 0 112 245 144 887.3 0 144 246 154 887.3 0 154 247 221 887.3 0 221 248 373 887.3 0 373 249 362 887.3 0 362 250 382 887.3 0 382 251 1645 887.3 0 1645 252 1124 887.3 0 1124 253 2503 887.3 0 2503 254 184 887.3 0 184 255 171 887.3 0 171 256 74 887.3 0 74 257 104 887.3 0 104 258 133 887.3 0 133 259 144 887.3 0 144 260 414 887.3 0 414 261 533 887.3 0 533 262 612 887.3 0 612 263 319 887.3 0 319 264 181 887.3 0 181 265 103 887.3 0 103 266 73 887.3 0 73 267 94 887.3 0 94 268 181 887.3 0 181 269 176 887.3 0 176 270 157 887.3 0 157 271 123 887.3 0 123 272 158 887.3 0 158 273 178 887.3 0 178 274 183 887.3 0 183 275 113 887.3 0 113 276 130 887.3 0 130 277 180 887.3 0 180 278 203 887.3 0 203 279 291 887.3 0 291 280 193 887.3 0 193 281 334 887.3 0 334 282 312 887.3 0 312 283 216 887.3 0 216 284 118 887.3 0 118 285 244 887.3 0 244 286 181 887.3 0 181 287 139 887.3 0 139 288 129 887.3 0 129 289 112 887.3 0 112 290 158 887.3 0 158 291 308 887.3 0 308 292 281 887.3 0 281 293 182 887.3 0 182 294 176 887.3 0 176 295 321 887.3 0 321 296 209 887.3 0 209 297 274 887.3 0 274 298 267 887.3 0 267 299 343 887.3 0 343 300 306 887.3 0 306 301 308 887.3 0 308 Finished in 22.61 s (78 us/read; 0.77 M reads/minute). === Summary === Total reads processed: 289,133 Reads with adapters: 119,268 (41.3%) Reads that were too short: 90 (0.0%) Reads written (passing filters): 119,178 (41.2%) Total basepairs processed: 77,951,724 bp Total written (filtered): 32,381,145 bp (41.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 119268 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 15 0.0 3 0 0 0 4 11 18 71 0.0 3 0 0 52 13 6 19 1271 0.0 3 0 1139 64 48 20 20 116669 0.0 4 111567 4163 671 192 76 21 285 0.0 4 60 197 20 4 4 22 23 0.0 4 2 0 18 0 3 23 408 0.0 4 158 4 2 179 65 24 3 0.0 4 0 1 0 0 2 25 2 0.0 4 2 26 1 0.0 4 0 0 0 1 34 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 1 41 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 55 3 0.0 4 0 0 0 0 3 87 1 0.0 4 0 0 0 0 1 103 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 8 0.0 4 0 0 0 0 8 127 410 0.0 4 0 0 0 0 410 129 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 1 1 155 15 0.0 4 0 0 0 0 15 157 1 0.0 4 0 0 0 0 1 210 1 0.0 4 1 212 2 0.0 4 0 0 0 0 2 221 2 0.0 4 1 0 0 0 1 222 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 0 0 2 228 12 0.0 4 0 0 0 0 12 229 9 0.0 4 0 0 0 1 8 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 0 3 235 8 0.0 4 0 0 0 0 8 237 7 0.0 4 0 0 0 0 7 241 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 268 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 7.46 s (63 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 119,178 Reads with adapters: 25,218 (21.2%) Reads written (passing filters): 119,178 (100.0%) Total basepairs processed: 32,381,145 bp Total written (filtered): 32,119,138 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 25218 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 5.8% G: 91.1% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 93 1862.2 0 93 4 27 465.5 0 27 5 5 116.4 0 5 6 2 29.1 0 2 7 3 7.3 0 3 8 12 1.8 0 12 9 5720 0.5 0 300 5420 10 8558 0.1 1 468 8090 11 10668 0.0 1 202 10466 12 57 0.0 1 3 54 13 27 0.0 1 0 27 14 8 0.0 1 0 8 15 1 0.0 1 0 1 21 1 0.0 1 1 39 1 0.0 1 1 74 1 0.0 1 1 80 1 0.0 1 1 87 1 0.0 1 1 91 1 0.0 1 0 1 150 17 0.0 1 12 5 151 3 0.0 1 3 190 1 0.0 1 0 1 208 5 0.0 1 4 1 243 1 0.0 1 1 257 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.