This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 178.58 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 295,612 (8.1%) Reads that were too short: 11,290 (0.3%) Reads written (passing filters): 284,322 (7.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 78,880,337 bp (7.2%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 295612 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1826 887.3 0 1826 7 9573 887.3 0 9573 8 115045 887.3 0 115045 9 122688 887.3 0 122688 10 153 887.3 0 153 11 56 887.3 0 56 12 52 887.3 0 52 13 40 887.3 0 40 14 39 887.3 0 39 15 46 887.3 0 46 16 37 887.3 0 37 17 35 887.3 0 35 18 41 887.3 0 41 19 49 887.3 0 49 20 34 887.3 0 34 21 54 887.3 0 54 22 45 887.3 0 45 23 43 887.3 0 43 24 53 887.3 0 53 25 50 887.3 0 50 26 41 887.3 0 41 27 46 887.3 0 46 28 60 887.3 0 60 29 42 887.3 0 42 30 38 887.3 0 38 31 72 887.3 0 72 32 45 887.3 0 45 33 30 887.3 0 30 34 62 887.3 0 62 35 37 887.3 0 37 36 56 887.3 0 56 37 59 887.3 0 59 38 48 887.3 0 48 39 48 887.3 0 48 40 57 887.3 0 57 41 50 887.3 0 50 42 31 887.3 0 31 43 32 887.3 0 32 44 35 887.3 0 35 45 34 887.3 0 34 46 49 887.3 0 49 47 48 887.3 0 48 48 44 887.3 0 44 49 49 887.3 0 49 50 43 887.3 0 43 51 23 887.3 0 23 52 36 887.3 0 36 53 43 887.3 0 43 54 81 887.3 0 81 55 63 887.3 0 63 56 2159 887.3 0 2159 57 70 887.3 0 70 58 56 887.3 0 56 59 39 887.3 0 39 60 55 887.3 0 55 61 44 887.3 0 44 62 41 887.3 0 41 63 42 887.3 0 42 64 62 887.3 0 62 65 66 887.3 0 66 66 36 887.3 0 36 67 92 887.3 0 92 68 80 887.3 0 80 69 73 887.3 0 73 70 70 887.3 0 70 71 100 887.3 0 100 72 708 887.3 0 708 73 1867 887.3 0 1867 74 1800 887.3 0 1800 75 854 887.3 0 854 76 43 887.3 0 43 77 31 887.3 0 31 78 37 887.3 0 37 79 54 887.3 0 54 80 39 887.3 0 39 81 34 887.3 0 34 82 37 887.3 0 37 83 44 887.3 0 44 84 48 887.3 0 48 85 63 887.3 0 63 86 73 887.3 0 73 87 69 887.3 0 69 88 86 887.3 0 86 89 76 887.3 0 76 90 71 887.3 0 71 91 49 887.3 0 49 92 38 887.3 0 38 93 42 887.3 0 42 94 67 887.3 0 67 95 46 887.3 0 46 96 43 887.3 0 43 97 36 887.3 0 36 98 30 887.3 0 30 99 39 887.3 0 39 100 46 887.3 0 46 101 35 887.3 0 35 102 42 887.3 0 42 103 35 887.3 0 35 104 31 887.3 0 31 105 75 887.3 0 75 106 51 887.3 0 51 107 52 887.3 0 52 108 65 887.3 0 65 109 67 887.3 0 67 110 59 887.3 0 59 111 51 887.3 0 51 112 45 887.3 0 45 113 37 887.3 0 37 114 29 887.3 0 29 115 55 887.3 0 55 116 65 887.3 0 65 117 48 887.3 0 48 118 57 887.3 0 57 119 51 887.3 0 51 120 47 887.3 0 47 121 34 887.3 0 34 122 46 887.3 0 46 123 34 887.3 0 34 124 34 887.3 0 34 125 44 887.3 0 44 126 50 887.3 0 50 127 36 887.3 0 36 128 60 887.3 0 60 129 81 887.3 0 81 130 52 887.3 0 52 131 58 887.3 0 58 132 38 887.3 0 38 133 59 887.3 0 59 134 55 887.3 0 55 135 38 887.3 0 38 136 99 887.3 0 99 137 62 887.3 0 62 138 50 887.3 0 50 139 43 887.3 0 43 140 45 887.3 0 45 141 55 887.3 0 55 142 169 887.3 0 169 143 78 887.3 0 78 144 144 887.3 0 144 145 216 887.3 0 216 146 2197 887.3 0 2197 147 1324 887.3 0 1324 148 757 887.3 0 757 149 88 887.3 0 88 150 92 887.3 0 92 151 107 887.3 0 107 152 38 887.3 0 38 153 50 887.3 0 50 154 58 887.3 0 58 155 95 887.3 0 95 156 134 887.3 0 134 157 115 887.3 0 115 158 725 887.3 0 725 159 459 887.3 0 459 160 411 887.3 0 411 161 169 887.3 0 169 162 242 887.3 0 242 163 452 887.3 0 452 164 59 887.3 0 59 165 43 887.3 0 43 166 44 887.3 0 44 167 56 887.3 0 56 168 64 887.3 0 64 169 95 887.3 0 95 170 153 887.3 0 153 171 141 887.3 0 141 172 110 887.3 0 110 173 130 887.3 0 130 174 1585 887.3 0 1585 175 1825 887.3 0 1825 176 1476 887.3 0 1476 177 109 887.3 0 109 178 81 887.3 0 81 179 97 887.3 0 97 180 834 887.3 0 834 181 2196 887.3 0 2196 182 1339 887.3 0 1339 183 44 887.3 0 44 184 76 887.3 0 76 185 75 887.3 0 75 186 129 887.3 0 129 187 923 887.3 0 923 188 572 887.3 0 572 189 389 887.3 0 389 190 62 887.3 0 62 191 53 887.3 0 53 192 45 887.3 0 45 193 60 887.3 0 60 194 47 887.3 0 47 195 35 887.3 0 35 196 39 887.3 0 39 197 36 887.3 0 36 198 44 887.3 0 44 199 40 887.3 0 40 200 57 887.3 0 57 201 53 887.3 0 53 202 31 887.3 0 31 203 40 887.3 0 40 204 132 887.3 0 132 205 135 887.3 0 135 206 86 887.3 0 86 207 50 887.3 0 50 208 41 887.3 0 41 209 36 887.3 0 36 210 34 887.3 0 34 211 52 887.3 0 52 212 41 887.3 0 41 213 33 887.3 0 33 214 50 887.3 0 50 215 84 887.3 0 84 216 43 887.3 0 43 217 45 887.3 0 45 218 68 887.3 0 68 219 59 887.3 0 59 220 56 887.3 0 56 221 59 887.3 0 59 222 38 887.3 0 38 223 59 887.3 0 59 224 59 887.3 0 59 225 61 887.3 0 61 226 35 887.3 0 35 227 82 887.3 0 82 228 116 887.3 0 116 229 154 887.3 0 154 230 75 887.3 0 75 231 84 887.3 0 84 232 63 887.3 0 63 233 70 887.3 0 70 234 62 887.3 0 62 235 57 887.3 0 57 236 76 887.3 0 76 237 88 887.3 0 88 238 104 887.3 0 104 239 103 887.3 0 103 240 54 887.3 0 54 241 33 887.3 0 33 242 53 887.3 0 53 243 47 887.3 0 47 244 61 887.3 0 61 245 61 887.3 0 61 246 55 887.3 0 55 247 82 887.3 0 82 248 119 887.3 0 119 249 140 887.3 0 140 250 166 887.3 0 166 251 1033 887.3 0 1033 252 530 887.3 0 530 253 1378 887.3 0 1378 254 90 887.3 0 90 255 70 887.3 0 70 256 63 887.3 0 63 257 59 887.3 0 59 258 66 887.3 0 66 259 63 887.3 0 63 260 193 887.3 0 193 261 219 887.3 0 219 262 234 887.3 0 234 263 140 887.3 0 140 264 82 887.3 0 82 265 55 887.3 0 55 266 35 887.3 0 35 267 38 887.3 0 38 268 82 887.3 0 82 269 86 887.3 0 86 270 80 887.3 0 80 271 56 887.3 0 56 272 85 887.3 0 85 273 94 887.3 0 94 274 85 887.3 0 85 275 97 887.3 0 97 276 81 887.3 0 81 277 92 887.3 0 92 278 89 887.3 0 89 279 100 887.3 0 100 280 81 887.3 0 81 281 107 887.3 0 107 282 95 887.3 0 95 283 68 887.3 0 68 284 65 887.3 0 65 285 196 887.3 0 196 286 99 887.3 0 99 287 63 887.3 0 63 288 70 887.3 0 70 289 62 887.3 0 62 290 75 887.3 0 75 291 104 887.3 0 104 292 105 887.3 0 105 293 67 887.3 0 67 294 126 887.3 0 126 295 263 887.3 0 263 296 99 887.3 0 99 297 122 887.3 0 122 298 110 887.3 0 110 299 116 887.3 0 116 300 241 887.3 0 241 301 149 887.3 0 149 Finished in 21.04 s (74 us/read; 0.81 M reads/minute). === Summary === Total reads processed: 284,322 Reads with adapters: 118,386 (41.6%) Reads that were too short: 141 (0.0%) Reads written (passing filters): 118,245 (41.6%) Total basepairs processed: 78,880,337 bp Total written (filtered): 32,637,069 bp (41.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 118386 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 7 0.0 2 0 0 0 7 15 21 0.0 3 0 0 20 1 16 822 0.0 3 0 800 19 3 17 116910 0.0 3 114724 2126 51 9 18 263 0.0 3 38 217 8 19 19 0.0 3 0 0 17 2 20 153 0.0 3 1 1 0 151 21 1 0.0 3 1 22 1 0.0 3 1 25 1 0.0 3 1 26 1 0.0 3 1 30 3 0.0 3 2 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 1 35 1 0.0 3 1 39 1 0.0 3 0 0 0 1 41 1 0.0 3 1 45 2 0.0 3 0 0 0 2 46 21 0.0 3 0 0 0 21 47 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 4 0.0 3 0 0 0 4 99 1 0.0 3 0 0 0 1 106 74 0.0 3 0 0 0 74 108 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 0 2 129 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 171 3 0.0 3 0 0 0 3 180 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 1 0 0 1 193 2 0.0 3 0 0 0 2 194 10 0.0 3 0 0 0 10 195 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 222 1 0.0 3 0 0 0 1 223 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 270 3 0.0 3 0 0 0 3 271 2 0.0 3 0 0 0 2 278 4 0.0 3 0 0 0 4 279 6 0.0 3 0 0 0 6 280 4 0.0 3 0 0 0 4 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.84 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 118,245 Reads with adapters: 456 (0.4%) Reads written (passing filters): 118,245 (100.0%) Total basepairs processed: 32,637,069 bp Total written (filtered): 32,635,606 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 456 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 77.2% C: 7.7% G: 11.6% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 374 1847.6 0 374 4 75 461.9 0 75 5 6 115.5 0 6 11 1 0.0 1 0 1