This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 173.64 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 122,750 (3.4%) Reads that were too short: 22,615 (0.6%) Reads written (passing filters): 100,135 (2.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 18,437,473 bp (1.7%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 122750 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 652 887.3 0 652 7 16197 887.3 0 16197 8 13904 887.3 0 13904 9 54 887.3 0 54 10 171 887.3 0 171 11 632 887.3 0 632 12 140 887.3 0 140 13 308 887.3 0 308 14 215 887.3 0 215 15 366 887.3 0 366 16 69 887.3 0 69 17 18 887.3 0 18 18 23 887.3 0 23 19 24 887.3 0 24 20 18 887.3 0 18 21 21 887.3 0 21 22 22 887.3 0 22 23 33 887.3 0 33 24 50 887.3 0 50 25 109 887.3 0 109 26 127 887.3 0 127 27 178 887.3 0 178 28 27 887.3 0 27 29 18 887.3 0 18 30 25 887.3 0 25 31 26 887.3 0 26 32 19 887.3 0 19 33 25 887.3 0 25 34 16 887.3 0 16 35 36 887.3 0 36 36 30 887.3 0 30 37 15 887.3 0 15 38 38 887.3 0 38 39 29 887.3 0 29 40 22 887.3 0 22 41 20 887.3 0 20 42 19 887.3 0 19 43 30 887.3 0 30 44 24 887.3 0 24 45 28 887.3 0 28 46 28 887.3 0 28 47 45 887.3 0 45 48 25 887.3 0 25 49 27 887.3 0 27 50 41 887.3 0 41 51 28 887.3 0 28 52 22 887.3 0 22 53 37 887.3 0 37 54 31 887.3 0 31 55 42 887.3 0 42 56 34 887.3 0 34 57 35 887.3 0 35 58 33 887.3 0 33 59 59 887.3 0 59 60 103 887.3 0 103 61 97 887.3 0 97 62 64 887.3 0 64 63 23 887.3 0 23 64 31 887.3 0 31 65 33 887.3 0 33 66 41 887.3 0 41 67 32 887.3 0 32 68 25 887.3 0 25 69 28 887.3 0 28 70 34 887.3 0 34 71 26 887.3 0 26 72 29 887.3 0 29 73 33 887.3 0 33 74 32 887.3 0 32 75 35 887.3 0 35 76 37 887.3 0 37 77 44 887.3 0 44 78 108 887.3 0 108 79 57 887.3 0 57 80 332 887.3 0 332 81 114 887.3 0 114 82 599 887.3 0 599 83 347 887.3 0 347 84 1195 887.3 0 1195 85 28 887.3 0 28 86 47 887.3 0 47 87 49 887.3 0 49 88 30 887.3 0 30 89 67 887.3 0 67 90 34 887.3 0 34 91 25 887.3 0 25 92 28 887.3 0 28 93 68 887.3 0 68 94 26 887.3 0 26 95 63 887.3 0 63 96 98 887.3 0 98 97 54 887.3 0 54 98 40 887.3 0 40 99 86 887.3 0 86 100 60 887.3 0 60 101 44 887.3 0 44 102 91 887.3 0 91 103 113 887.3 0 113 104 144 887.3 0 144 105 79 887.3 0 79 106 87 887.3 0 87 107 374 887.3 0 374 108 41 887.3 0 41 109 34 887.3 0 34 110 121 887.3 0 121 111 94 887.3 0 94 112 133 887.3 0 133 113 105 887.3 0 105 114 98 887.3 0 98 115 60 887.3 0 60 116 45 887.3 0 45 117 45 887.3 0 45 118 79 887.3 0 79 119 91 887.3 0 91 120 110 887.3 0 110 121 76 887.3 0 76 122 173 887.3 0 173 123 233 887.3 0 233 124 143 887.3 0 143 125 72 887.3 0 72 126 83 887.3 0 83 127 70 887.3 0 70 128 80 887.3 0 80 129 60 887.3 0 60 130 71 887.3 0 71 131 54 887.3 0 54 132 80 887.3 0 80 133 50 887.3 0 50 134 118 887.3 0 118 135 99 887.3 0 99 136 92 887.3 0 92 137 146 887.3 0 146 138 186 887.3 0 186 139 141 887.3 0 141 140 162 887.3 0 162 141 167 887.3 0 167 142 134 887.3 0 134 143 65 887.3 0 65 144 71 887.3 0 71 145 91 887.3 0 91 146 122 887.3 0 122 147 146 887.3 0 146 148 100 887.3 0 100 149 92 887.3 0 92 150 77 887.3 0 77 151 85 887.3 0 85 152 98 887.3 0 98 153 98 887.3 0 98 154 93 887.3 0 93 155 157 887.3 0 157 156 123 887.3 0 123 157 166 887.3 0 166 158 180 887.3 0 180 159 359 887.3 0 359 160 1806 887.3 0 1806 161 1661 887.3 0 1661 162 1611 887.3 0 1611 163 211 887.3 0 211 164 109 887.3 0 109 165 94 887.3 0 94 166 84 887.3 0 84 167 83 887.3 0 83 168 103 887.3 0 103 169 99 887.3 0 99 170 186 887.3 0 186 171 64 887.3 0 64 172 88 887.3 0 88 173 105 887.3 0 105 174 121 887.3 0 121 175 227 887.3 0 227 176 137 887.3 0 137 177 143 887.3 0 143 178 105 887.3 0 105 179 121 887.3 0 121 180 426 887.3 0 426 181 758 887.3 0 758 182 1103 887.3 0 1103 183 704 887.3 0 704 184 424 887.3 0 424 185 345 887.3 0 345 186 615 887.3 0 615 187 7548 887.3 0 7548 188 4371 887.3 0 4371 189 30461 887.3 0 30461 190 185 887.3 0 185 191 75 887.3 0 75 192 71 887.3 0 71 193 65 887.3 0 65 194 64 887.3 0 64 195 65 887.3 0 65 196 77 887.3 0 77 197 63 887.3 0 63 198 81 887.3 0 81 199 100 887.3 0 100 200 115 887.3 0 115 201 93 887.3 0 93 202 138 887.3 0 138 203 154 887.3 0 154 204 193 887.3 0 193 205 301 887.3 0 301 206 306 887.3 0 306 207 251 887.3 0 251 208 116 887.3 0 116 209 107 887.3 0 107 210 817 887.3 0 817 211 852 887.3 0 852 212 1087 887.3 0 1087 213 206 887.3 0 206 214 106 887.3 0 106 215 69 887.3 0 69 216 68 887.3 0 68 217 100 887.3 0 100 218 80 887.3 0 80 219 209 887.3 0 209 220 293 887.3 0 293 221 265 887.3 0 265 222 118 887.3 0 118 223 103 887.3 0 103 224 116 887.3 0 116 225 143 887.3 0 143 226 126 887.3 0 126 227 170 887.3 0 170 228 134 887.3 0 134 229 217 887.3 0 217 230 1108 887.3 0 1108 231 1089 887.3 0 1089 232 550 887.3 0 550 233 148 887.3 0 148 234 76 887.3 0 76 235 137 887.3 0 137 236 126 887.3 0 126 237 77 887.3 0 77 238 61 887.3 0 61 239 80 887.3 0 80 240 130 887.3 0 130 241 106 887.3 0 106 242 76 887.3 0 76 243 65 887.3 0 65 244 63 887.3 0 63 245 74 887.3 0 74 246 72 887.3 0 72 247 47 887.3 0 47 248 53 887.3 0 53 249 71 887.3 0 71 250 67 887.3 0 67 251 70 887.3 0 70 252 98 887.3 0 98 253 145 887.3 0 145 254 134 887.3 0 134 255 101 887.3 0 101 256 99 887.3 0 99 257 99 887.3 0 99 258 121 887.3 0 121 259 108 887.3 0 108 260 100 887.3 0 100 261 77 887.3 0 77 262 88 887.3 0 88 263 91 887.3 0 91 264 141 887.3 0 141 265 80 887.3 0 80 266 89 887.3 0 89 267 128 887.3 0 128 268 152 887.3 0 152 269 167 887.3 0 167 270 85 887.3 0 85 271 86 887.3 0 86 272 100 887.3 0 100 273 168 887.3 0 168 274 161 887.3 0 161 275 502 887.3 0 502 276 520 887.3 0 520 277 479 887.3 0 479 278 339 887.3 0 339 279 379 887.3 0 379 280 249 887.3 0 249 281 191 887.3 0 191 282 151 887.3 0 151 283 199 887.3 0 199 284 218 887.3 0 218 285 223 887.3 0 223 286 113 887.3 0 113 287 217 887.3 0 217 288 319 887.3 0 319 289 749 887.3 0 749 290 605 887.3 0 605 291 705 887.3 0 705 292 86 887.3 0 86 293 125 887.3 0 125 294 144 887.3 0 144 295 145 887.3 0 145 296 98 887.3 0 98 297 121 887.3 0 121 298 188 887.3 0 188 299 241 887.3 0 241 300 770 887.3 0 770 301 730 887.3 0 730 Finished in 6.43 s (64 us/read; 0.93 M reads/minute). === Summary === Total reads processed: 100,135 Reads with adapters: 15,483 (15.5%) Reads that were too short: 29 (0.0%) Reads written (passing filters): 15,454 (15.4%) Total basepairs processed: 18,437,473 bp Total written (filtered): 4,124,203 bp (22.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15483 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 8 0.0 3 0 0 7 1 19 155 0.0 3 0 136 9 6 4 20 14294 0.0 4 13638 542 90 16 8 21 41 0.0 4 10 26 2 2 1 22 17 0.0 4 12 2 2 1 23 36 0.0 4 0 0 0 25 11 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 27 1 0.0 4 1 29 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 1 52 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 123 10 0.0 4 0 0 0 0 10 125 1 0.0 4 0 0 0 0 1 126 12 0.0 4 0 0 0 0 12 127 864 0.0 4 0 0 0 0 864 128 3 0.0 4 0 1 0 0 2 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 142 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 232 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.94 s (61 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 15,454 Reads with adapters: 2,953 (19.1%) Reads written (passing filters): 15,454 (100.0%) Total basepairs processed: 4,124,203 bp Total written (filtered): 4,091,307 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2953 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.0% C: 5.8% G: 90.8% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 20 241.5 0 20 4 17 60.4 0 17 5 1 15.1 0 1 6 1 3.8 0 1 9 35 0.1 0 1 34 10 212 0.0 1 10 202 11 2637 0.0 1 45 2592 12 20 0.0 1 1 19 13 3 0.0 1 0 3 14 1 0.0 1 0 1 63 1 0.0 1 1 151 2 0.0 1 2 209 1 0.0 1 1 224 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.