This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 173.97 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 113,062 (3.1%) Reads that were too short: 15,175 (0.4%) Reads written (passing filters): 97,887 (2.7%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 18,009,536 bp (1.6%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 113062 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 439 887.3 0 439 7 14835 887.3 0 14835 8 16142 887.3 0 16142 9 28 887.3 0 28 10 30 887.3 0 30 11 167 887.3 0 167 12 39 887.3 0 39 13 87 887.3 0 87 14 138 887.3 0 138 15 158 887.3 0 158 16 55 887.3 0 55 17 11 887.3 0 11 18 15 887.3 0 15 19 12 887.3 0 12 20 14 887.3 0 14 21 15 887.3 0 15 22 7 887.3 0 7 23 17 887.3 0 17 24 19 887.3 0 19 25 980 887.3 0 980 26 59 887.3 0 59 27 20 887.3 0 20 28 13 887.3 0 13 29 14 887.3 0 14 30 13 887.3 0 13 31 15 887.3 0 15 32 20 887.3 0 20 33 19 887.3 0 19 34 18 887.3 0 18 35 15 887.3 0 15 36 23 887.3 0 23 37 9 887.3 0 9 38 10 887.3 0 10 39 14 887.3 0 14 40 13 887.3 0 13 41 44 887.3 0 44 42 19 887.3 0 19 43 17 887.3 0 17 44 25 887.3 0 25 45 19 887.3 0 19 46 20 887.3 0 20 47 19 887.3 0 19 48 15 887.3 0 15 49 19 887.3 0 19 50 19 887.3 0 19 51 24 887.3 0 24 52 21 887.3 0 21 53 24 887.3 0 24 54 15 887.3 0 15 55 28 887.3 0 28 56 34 887.3 0 34 57 45 887.3 0 45 58 24 887.3 0 24 59 45 887.3 0 45 60 78 887.3 0 78 61 55 887.3 0 55 62 46 887.3 0 46 63 17 887.3 0 17 64 20 887.3 0 20 65 27 887.3 0 27 66 25 887.3 0 25 67 18 887.3 0 18 68 13 887.3 0 13 69 18 887.3 0 18 70 24 887.3 0 24 71 26 887.3 0 26 72 14 887.3 0 14 73 16 887.3 0 16 74 28 887.3 0 28 75 27 887.3 0 27 76 28 887.3 0 28 77 24 887.3 0 24 78 29 887.3 0 29 79 50 887.3 0 50 80 67 887.3 0 67 81 129 887.3 0 129 82 940 887.3 0 940 83 508 887.3 0 508 84 1910 887.3 0 1910 85 42 887.3 0 42 86 67 887.3 0 67 87 70 887.3 0 70 88 46 887.3 0 46 89 87 887.3 0 87 90 61 887.3 0 61 91 46 887.3 0 46 92 35 887.3 0 35 93 55 887.3 0 55 94 54 887.3 0 54 95 71 887.3 0 71 96 66 887.3 0 66 97 47 887.3 0 47 98 60 887.3 0 60 99 40 887.3 0 40 100 47 887.3 0 47 101 69 887.3 0 69 102 60 887.3 0 60 103 58 887.3 0 58 104 95 887.3 0 95 105 81 887.3 0 81 106 85 887.3 0 85 107 395 887.3 0 395 108 31 887.3 0 31 109 52 887.3 0 52 110 116 887.3 0 116 111 76 887.3 0 76 112 86 887.3 0 86 113 73 887.3 0 73 114 69 887.3 0 69 115 53 887.3 0 53 116 45 887.3 0 45 117 45 887.3 0 45 118 79 887.3 0 79 119 71 887.3 0 71 120 97 887.3 0 97 121 67 887.3 0 67 122 123 887.3 0 123 123 152 887.3 0 152 124 99 887.3 0 99 125 74 887.3 0 74 126 86 887.3 0 86 127 77 887.3 0 77 128 59 887.3 0 59 129 61 887.3 0 61 130 57 887.3 0 57 131 86 887.3 0 86 132 76 887.3 0 76 133 49 887.3 0 49 134 91 887.3 0 91 135 55 887.3 0 55 136 64 887.3 0 64 137 86 887.3 0 86 138 87 887.3 0 87 139 95 887.3 0 95 140 124 887.3 0 124 141 112 887.3 0 112 142 102 887.3 0 102 143 69 887.3 0 69 144 81 887.3 0 81 145 86 887.3 0 86 146 88 887.3 0 88 147 111 887.3 0 111 148 59 887.3 0 59 149 68 887.3 0 68 150 78 887.3 0 78 151 51 887.3 0 51 152 60 887.3 0 60 153 83 887.3 0 83 154 82 887.3 0 82 155 71 887.3 0 71 156 78 887.3 0 78 157 80 887.3 0 80 158 112 887.3 0 112 159 134 887.3 0 134 160 507 887.3 0 507 161 545 887.3 0 545 162 569 887.3 0 569 163 108 887.3 0 108 164 60 887.3 0 60 165 60 887.3 0 60 166 71 887.3 0 71 167 67 887.3 0 67 168 66 887.3 0 66 169 103 887.3 0 103 170 173 887.3 0 173 171 75 887.3 0 75 172 73 887.3 0 73 173 100 887.3 0 100 174 103 887.3 0 103 175 163 887.3 0 163 176 102 887.3 0 102 177 83 887.3 0 83 178 74 887.3 0 74 179 82 887.3 0 82 180 207 887.3 0 207 181 278 887.3 0 278 182 416 887.3 0 416 183 259 887.3 0 259 184 366 887.3 0 366 185 284 887.3 0 284 186 538 887.3 0 538 187 8251 887.3 0 8251 188 4396 887.3 0 4396 189 34835 887.3 0 34835 190 165 887.3 0 165 191 70 887.3 0 70 192 51 887.3 0 51 193 52 887.3 0 52 194 60 887.3 0 60 195 54 887.3 0 54 196 55 887.3 0 55 197 72 887.3 0 72 198 80 887.3 0 80 199 70 887.3 0 70 200 74 887.3 0 74 201 71 887.3 0 71 202 100 887.3 0 100 203 104 887.3 0 104 204 93 887.3 0 93 205 145 887.3 0 145 206 132 887.3 0 132 207 148 887.3 0 148 208 83 887.3 0 83 209 57 887.3 0 57 210 307 887.3 0 307 211 321 887.3 0 321 212 468 887.3 0 468 213 120 887.3 0 120 214 87 887.3 0 87 215 81 887.3 0 81 216 70 887.3 0 70 217 59 887.3 0 59 218 71 887.3 0 71 219 121 887.3 0 121 220 221 887.3 0 221 221 176 887.3 0 176 222 79 887.3 0 79 223 74 887.3 0 74 224 86 887.3 0 86 225 98 887.3 0 98 226 77 887.3 0 77 227 124 887.3 0 124 228 83 887.3 0 83 229 125 887.3 0 125 230 587 887.3 0 587 231 558 887.3 0 558 232 277 887.3 0 277 233 119 887.3 0 119 234 63 887.3 0 63 235 110 887.3 0 110 236 105 887.3 0 105 237 63 887.3 0 63 238 56 887.3 0 56 239 98 887.3 0 98 240 94 887.3 0 94 241 97 887.3 0 97 242 75 887.3 0 75 243 64 887.3 0 64 244 84 887.3 0 84 245 89 887.3 0 89 246 66 887.3 0 66 247 58 887.3 0 58 248 55 887.3 0 55 249 64 887.3 0 64 250 56 887.3 0 56 251 73 887.3 0 73 252 89 887.3 0 89 253 105 887.3 0 105 254 97 887.3 0 97 255 83 887.3 0 83 256 81 887.3 0 81 257 73 887.3 0 73 258 96 887.3 0 96 259 67 887.3 0 67 260 92 887.3 0 92 261 71 887.3 0 71 262 68 887.3 0 68 263 80 887.3 0 80 264 117 887.3 0 117 265 68 887.3 0 68 266 78 887.3 0 78 267 66 887.3 0 66 268 79 887.3 0 79 269 107 887.3 0 107 270 56 887.3 0 56 271 78 887.3 0 78 272 70 887.3 0 70 273 123 887.3 0 123 274 146 887.3 0 146 275 460 887.3 0 460 276 505 887.3 0 505 277 463 887.3 0 463 278 259 887.3 0 259 279 224 887.3 0 224 280 160 887.3 0 160 281 125 887.3 0 125 282 83 887.3 0 83 283 112 887.3 0 112 284 145 887.3 0 145 285 134 887.3 0 134 286 86 887.3 0 86 287 122 887.3 0 122 288 259 887.3 0 259 289 484 887.3 0 484 290 402 887.3 0 402 291 506 887.3 0 506 292 74 887.3 0 74 293 103 887.3 0 103 294 101 887.3 0 101 295 103 887.3 0 103 296 104 887.3 0 104 297 148 887.3 0 148 298 178 887.3 0 178 299 234 887.3 0 234 300 702 887.3 0 702 301 288 887.3 0 288 Finished in 5.90 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 97,887 Reads with adapters: 14,697 (15.0%) Reads that were too short: 39 (0.0%) Reads written (passing filters): 14,658 (15.0%) Total basepairs processed: 18,009,536 bp Total written (filtered): 4,046,193 bp (22.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14697 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 6 0.0 3 0 0 6 16 119 0.0 3 0 118 1 17 14388 0.0 3 14125 254 9 18 41 0.0 3 3 38 19 8 0.0 3 3 0 4 1 20 10 0.0 3 0 0 0 10 22 1 0.0 3 1 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 33 2 0.0 3 0 0 0 2 35 1 0.0 3 1 37 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 2 0.0 3 0 0 0 2 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 57 1 0.0 3 0 0 0 1 59 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 99 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 123 4 0.0 3 0 0 0 4 125 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 141 72 0.0 3 0 0 0 72 163 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 14,658 Reads with adapters: 12 (0.1%) Reads written (passing filters): 14,658 (100.0%) Total basepairs processed: 4,046,193 bp Total written (filtered): 4,046,155 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 12 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.3% C: 50.0% G: 16.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 229.0 0 11 5 1 14.3 0 1