This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 173.48 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 133,950 (3.7%) Reads that were too short: 33,646 (0.9%) Reads written (passing filters): 100,304 (2.8%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 20,236,364 bp (1.8%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 133950 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1395 887.3 0 1395 7 15249 887.3 0 15249 8 940 887.3 0 940 9 18181 887.3 0 18181 10 43 887.3 0 43 11 29 887.3 0 29 12 68 887.3 0 68 13 47 887.3 0 47 14 30 887.3 0 30 15 24 887.3 0 24 16 36 887.3 0 36 17 41 887.3 0 41 18 24 887.3 0 24 19 35 887.3 0 35 20 31 887.3 0 31 21 23 887.3 0 23 22 23 887.3 0 23 23 30 887.3 0 30 24 25 887.3 0 25 25 27 887.3 0 27 26 24 887.3 0 24 27 37 887.3 0 37 28 37 887.3 0 37 29 36 887.3 0 36 30 38 887.3 0 38 31 36 887.3 0 36 32 42 887.3 0 42 33 21 887.3 0 21 34 32 887.3 0 32 35 51 887.3 0 51 36 44 887.3 0 44 37 52 887.3 0 52 38 311 887.3 0 311 39 172 887.3 0 172 40 181 887.3 0 181 41 65 887.3 0 65 42 37 887.3 0 37 43 60 887.3 0 60 44 27 887.3 0 27 45 43 887.3 0 43 46 27 887.3 0 27 47 50 887.3 0 50 48 25 887.3 0 25 49 30 887.3 0 30 50 52 887.3 0 52 51 39 887.3 0 39 52 34 887.3 0 34 53 171 887.3 0 171 54 305 887.3 0 305 55 134 887.3 0 134 56 76 887.3 0 76 57 222 887.3 0 222 58 73 887.3 0 73 59 23 887.3 0 23 60 65 887.3 0 65 61 40 887.3 0 40 62 28 887.3 0 28 63 34 887.3 0 34 64 35 887.3 0 35 65 46 887.3 0 46 66 107 887.3 0 107 67 173 887.3 0 173 68 117 887.3 0 117 69 82 887.3 0 82 70 110 887.3 0 110 71 47 887.3 0 47 72 62 887.3 0 62 73 46 887.3 0 46 74 72 887.3 0 72 75 22 887.3 0 22 76 18 887.3 0 18 77 28 887.3 0 28 78 35 887.3 0 35 79 29 887.3 0 29 80 30 887.3 0 30 81 35 887.3 0 35 82 45 887.3 0 45 83 32 887.3 0 32 84 46 887.3 0 46 85 32 887.3 0 32 86 40 887.3 0 40 87 48 887.3 0 48 88 35 887.3 0 35 89 59 887.3 0 59 90 29 887.3 0 29 91 89 887.3 0 89 92 42 887.3 0 42 93 78 887.3 0 78 94 66 887.3 0 66 95 84 887.3 0 84 96 47 887.3 0 47 97 39 887.3 0 39 98 31 887.3 0 31 99 42 887.3 0 42 100 19 887.3 0 19 101 44 887.3 0 44 102 71 887.3 0 71 103 114 887.3 0 114 104 145 887.3 0 145 105 241 887.3 0 241 106 94 887.3 0 94 107 116 887.3 0 116 108 72 887.3 0 72 109 71 887.3 0 71 110 56 887.3 0 56 111 116 887.3 0 116 112 123 887.3 0 123 113 195 887.3 0 195 114 90 887.3 0 90 115 95 887.3 0 95 116 75 887.3 0 75 117 64 887.3 0 64 118 52 887.3 0 52 119 68 887.3 0 68 120 84 887.3 0 84 121 71 887.3 0 71 122 79 887.3 0 79 123 115 887.3 0 115 124 137 887.3 0 137 125 1094 887.3 0 1094 126 1465 887.3 0 1465 127 1747 887.3 0 1747 128 87 887.3 0 87 129 74 887.3 0 74 130 126 887.3 0 126 131 80 887.3 0 80 132 96 887.3 0 96 133 177 887.3 0 177 134 134 887.3 0 134 135 128 887.3 0 128 136 142 887.3 0 142 137 124 887.3 0 124 138 114 887.3 0 114 139 108 887.3 0 108 140 142 887.3 0 142 141 102 887.3 0 102 142 111 887.3 0 111 143 150 887.3 0 150 144 620 887.3 0 620 145 801 887.3 0 801 146 818 887.3 0 818 147 356 887.3 0 356 148 141 887.3 0 141 149 158 887.3 0 158 150 502 887.3 0 502 151 606 887.3 0 606 152 657 887.3 0 657 153 225 887.3 0 225 154 319 887.3 0 319 155 956 887.3 0 956 156 1069 887.3 0 1069 157 1320 887.3 0 1320 158 1383 887.3 0 1383 159 2288 887.3 0 2288 160 9984 887.3 0 9984 161 8177 887.3 0 8177 162 7642 887.3 0 7642 163 185 887.3 0 185 164 191 887.3 0 191 165 157 887.3 0 157 166 174 887.3 0 174 167 115 887.3 0 115 168 102 887.3 0 102 169 149 887.3 0 149 170 145 887.3 0 145 171 181 887.3 0 181 172 123 887.3 0 123 173 153 887.3 0 153 174 181 887.3 0 181 175 658 887.3 0 658 176 418 887.3 0 418 177 426 887.3 0 426 178 222 887.3 0 222 179 323 887.3 0 323 180 2049 887.3 0 2049 181 2335 887.3 0 2335 182 1627 887.3 0 1627 183 687 887.3 0 687 184 111 887.3 0 111 185 144 887.3 0 144 186 129 887.3 0 129 187 132 887.3 0 132 188 121 887.3 0 121 189 111 887.3 0 111 190 132 887.3 0 132 191 127 887.3 0 127 192 80 887.3 0 80 193 58 887.3 0 58 194 71 887.3 0 71 195 108 887.3 0 108 196 83 887.3 0 83 197 107 887.3 0 107 198 109 887.3 0 109 199 169 887.3 0 169 200 115 887.3 0 115 201 113 887.3 0 113 202 89 887.3 0 89 203 112 887.3 0 112 204 189 887.3 0 189 205 98 887.3 0 98 206 102 887.3 0 102 207 103 887.3 0 103 208 158 887.3 0 158 209 108 887.3 0 108 210 153 887.3 0 153 211 318 887.3 0 318 212 1701 887.3 0 1701 213 1830 887.3 0 1830 214 1403 887.3 0 1403 215 132 887.3 0 132 216 126 887.3 0 126 217 150 887.3 0 150 218 132 887.3 0 132 219 173 887.3 0 173 220 220 887.3 0 220 221 208 887.3 0 208 222 134 887.3 0 134 223 172 887.3 0 172 224 259 887.3 0 259 225 220 887.3 0 220 226 193 887.3 0 193 227 148 887.3 0 148 228 127 887.3 0 127 229 142 887.3 0 142 230 111 887.3 0 111 231 118 887.3 0 118 232 89 887.3 0 89 233 109 887.3 0 109 234 169 887.3 0 169 235 142 887.3 0 142 236 130 887.3 0 130 237 140 887.3 0 140 238 231 887.3 0 231 239 316 887.3 0 316 240 211 887.3 0 211 241 224 887.3 0 224 242 176 887.3 0 176 243 287 887.3 0 287 244 222 887.3 0 222 245 262 887.3 0 262 246 251 887.3 0 251 247 306 887.3 0 306 248 296 887.3 0 296 249 168 887.3 0 168 250 181 887.3 0 181 251 166 887.3 0 166 252 199 887.3 0 199 253 178 887.3 0 178 254 195 887.3 0 195 255 364 887.3 0 364 256 954 887.3 0 954 257 1275 887.3 0 1275 258 1070 887.3 0 1070 259 296 887.3 0 296 260 207 887.3 0 207 261 205 887.3 0 205 262 167 887.3 0 167 263 160 887.3 0 160 264 163 887.3 0 163 265 161 887.3 0 161 266 478 887.3 0 478 267 854 887.3 0 854 268 1019 887.3 0 1019 269 384 887.3 0 384 270 256 887.3 0 256 271 327 887.3 0 327 272 1097 887.3 0 1097 273 1398 887.3 0 1398 274 980 887.3 0 980 275 596 887.3 0 596 276 240 887.3 0 240 277 240 887.3 0 240 278 299 887.3 0 299 279 289 887.3 0 289 280 463 887.3 0 463 281 431 887.3 0 431 282 413 887.3 0 413 283 285 887.3 0 285 284 237 887.3 0 237 285 253 887.3 0 253 286 253 887.3 0 253 287 573 887.3 0 573 288 499 887.3 0 499 289 498 887.3 0 498 290 167 887.3 0 167 291 169 887.3 0 169 292 213 887.3 0 213 293 306 887.3 0 306 294 271 887.3 0 271 295 176 887.3 0 176 296 120 887.3 0 120 297 158 887.3 0 158 298 177 887.3 0 177 299 191 887.3 0 191 300 220 887.3 0 220 301 317 887.3 0 317 Finished in 6.82 s (68 us/read; 0.88 M reads/minute). === Summary === Total reads processed: 100,304 Reads with adapters: 16,519 (16.5%) Reads that were too short: 53 (0.1%) Reads written (passing filters): 16,466 (16.4%) Total basepairs processed: 20,236,364 bp Total written (filtered): 4,486,820 bp (22.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16519 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 122 0.0 3 0 0 12 2 108 19 261 0.0 3 0 234 18 8 1 20 15636 0.0 4 14915 614 78 25 4 21 330 0.0 4 282 40 4 2 2 22 8 0.0 4 2 1 4 1 23 23 0.0 4 0 0 0 21 2 24 1 0.0 4 1 29 2 0.0 4 0 0 0 0 2 34 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 60 12 0.0 4 0 0 0 0 12 61 5 0.0 4 0 0 0 1 4 62 4 0.0 4 0 0 0 0 4 67 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 1 85 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 127 73 0.0 4 0 0 0 0 73 128 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 223 2 0.0 4 0 0 0 0 2 228 3 0.0 4 0 0 0 0 3 229 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.95 s (58 us/read; 1.04 M reads/minute). === Summary === Total reads processed: 16,466 Reads with adapters: 920 (5.6%) Reads written (passing filters): 16,466 (100.0%) Total basepairs processed: 4,486,820 bp Total written (filtered): 4,476,894 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 920 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.3% C: 9.1% G: 82.9% T: 2.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 57 257.3 0 57 4 11 64.3 0 11 5 1 16.1 0 1 9 5 0.1 0 1 4 10 19 0.0 1 1 18 11 668 0.0 1 13 655 12 145 0.0 1 1 144 13 10 0.0 1 0 10 14 2 0.0 1 0 2 15 1 0.0 1 0 1 210 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.