This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 175.16 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 3,634,272 Reads with adapters: 109,344 (3.0%) Reads that were too short: 21,144 (0.6%) Reads written (passing filters): 88,200 (2.4%) Total basepairs processed: 1,093,915,872 bp Total written (filtered): 18,644,807 bp (1.7%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 109344 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1309 887.3 0 1309 7 18435 887.3 0 18435 8 774 887.3 0 774 9 16118 887.3 0 16118 10 26 887.3 0 26 11 26 887.3 0 26 12 32 887.3 0 32 13 27 887.3 0 27 14 35 887.3 0 35 15 22 887.3 0 22 16 19 887.3 0 19 17 26 887.3 0 26 18 25 887.3 0 25 19 21 887.3 0 21 20 24 887.3 0 24 21 33 887.3 0 33 22 26 887.3 0 26 23 20 887.3 0 20 24 25 887.3 0 25 25 23 887.3 0 23 26 21 887.3 0 21 27 23 887.3 0 23 28 31 887.3 0 31 29 35 887.3 0 35 30 24 887.3 0 24 31 24 887.3 0 24 32 23 887.3 0 23 33 23 887.3 0 23 34 102 887.3 0 102 35 55 887.3 0 55 36 34 887.3 0 34 37 45 887.3 0 45 38 268 887.3 0 268 39 213 887.3 0 213 40 196 887.3 0 196 41 40 887.3 0 40 42 15 887.3 0 15 43 25 887.3 0 25 44 22 887.3 0 22 45 284 887.3 0 284 46 34 887.3 0 34 47 25 887.3 0 25 48 21 887.3 0 21 49 15 887.3 0 15 50 39 887.3 0 39 51 23 887.3 0 23 52 19 887.3 0 19 53 173 887.3 0 173 54 303 887.3 0 303 55 121 887.3 0 121 56 32 887.3 0 32 57 211 887.3 0 211 58 29 887.3 0 29 59 22 887.3 0 22 60 44 887.3 0 44 61 31 887.3 0 31 62 25 887.3 0 25 63 43 887.3 0 43 64 36 887.3 0 36 65 28 887.3 0 28 66 39 887.3 0 39 67 57 887.3 0 57 68 34 887.3 0 34 69 93 887.3 0 93 70 113 887.3 0 113 71 35 887.3 0 35 72 29 887.3 0 29 73 29 887.3 0 29 74 24 887.3 0 24 75 14 887.3 0 14 76 27 887.3 0 27 77 23 887.3 0 23 78 21 887.3 0 21 79 24 887.3 0 24 80 18 887.3 0 18 81 27 887.3 0 27 82 22 887.3 0 22 83 38 887.3 0 38 84 29 887.3 0 29 85 30 887.3 0 30 86 40 887.3 0 40 87 39 887.3 0 39 88 65 887.3 0 65 89 63 887.3 0 63 90 46 887.3 0 46 91 83 887.3 0 83 92 56 887.3 0 56 93 68 887.3 0 68 94 62 887.3 0 62 95 71 887.3 0 71 96 43 887.3 0 43 97 34 887.3 0 34 98 39 887.3 0 39 99 30 887.3 0 30 100 35 887.3 0 35 101 51 887.3 0 51 102 48 887.3 0 48 103 51 887.3 0 51 104 109 887.3 0 109 105 104 887.3 0 104 106 75 887.3 0 75 107 62 887.3 0 62 108 54 887.3 0 54 109 58 887.3 0 58 110 41 887.3 0 41 111 72 887.3 0 72 112 73 887.3 0 73 113 129 887.3 0 129 114 51 887.3 0 51 115 69 887.3 0 69 116 60 887.3 0 60 117 45 887.3 0 45 118 56 887.3 0 56 119 59 887.3 0 59 120 84 887.3 0 84 121 68 887.3 0 68 122 77 887.3 0 77 123 114 887.3 0 114 124 104 887.3 0 104 125 1083 887.3 0 1083 126 1469 887.3 0 1469 127 1994 887.3 0 1994 128 61 887.3 0 61 129 61 887.3 0 61 130 80 887.3 0 80 131 67 887.3 0 67 132 49 887.3 0 49 133 127 887.3 0 127 134 91 887.3 0 91 135 72 887.3 0 72 136 113 887.3 0 113 137 61 887.3 0 61 138 51 887.3 0 51 139 45 887.3 0 45 140 61 887.3 0 61 141 73 887.3 0 73 142 82 887.3 0 82 143 108 887.3 0 108 144 369 887.3 0 369 145 489 887.3 0 489 146 468 887.3 0 468 147 206 887.3 0 206 148 101 887.3 0 101 149 100 887.3 0 100 150 361 887.3 0 361 151 430 887.3 0 430 152 414 887.3 0 414 153 137 887.3 0 137 154 187 887.3 0 187 155 462 887.3 0 462 156 537 887.3 0 537 157 631 887.3 0 631 158 698 887.3 0 698 159 1180 887.3 0 1180 160 10223 887.3 0 10223 161 8066 887.3 0 8066 162 7455 887.3 0 7455 163 127 887.3 0 127 164 133 887.3 0 133 165 88 887.3 0 88 166 104 887.3 0 104 167 47 887.3 0 47 168 61 887.3 0 61 169 104 887.3 0 104 170 71 887.3 0 71 171 147 887.3 0 147 172 65 887.3 0 65 173 63 887.3 0 63 174 92 887.3 0 92 175 290 887.3 0 290 176 182 887.3 0 182 177 197 887.3 0 197 178 91 887.3 0 91 179 125 887.3 0 125 180 1023 887.3 0 1023 181 1450 887.3 0 1450 182 806 887.3 0 806 183 372 887.3 0 372 184 66 887.3 0 66 185 109 887.3 0 109 186 80 887.3 0 80 187 84 887.3 0 84 188 77 887.3 0 77 189 62 887.3 0 62 190 63 887.3 0 63 191 57 887.3 0 57 192 62 887.3 0 62 193 51 887.3 0 51 194 52 887.3 0 52 195 64 887.3 0 64 196 48 887.3 0 48 197 88 887.3 0 88 198 74 887.3 0 74 199 88 887.3 0 88 200 74 887.3 0 74 201 64 887.3 0 64 202 65 887.3 0 65 203 89 887.3 0 89 204 180 887.3 0 180 205 58 887.3 0 58 206 54 887.3 0 54 207 83 887.3 0 83 208 86 887.3 0 86 209 84 887.3 0 84 210 119 887.3 0 119 211 244 887.3 0 244 212 1821 887.3 0 1821 213 1762 887.3 0 1762 214 1445 887.3 0 1445 215 72 887.3 0 72 216 63 887.3 0 63 217 89 887.3 0 89 218 90 887.3 0 90 219 93 887.3 0 93 220 156 887.3 0 156 221 144 887.3 0 144 222 73 887.3 0 73 223 71 887.3 0 71 224 98 887.3 0 98 225 109 887.3 0 109 226 114 887.3 0 114 227 112 887.3 0 112 228 76 887.3 0 76 229 86 887.3 0 86 230 92 887.3 0 92 231 81 887.3 0 81 232 62 887.3 0 62 233 77 887.3 0 77 234 84 887.3 0 84 235 94 887.3 0 94 236 101 887.3 0 101 237 103 887.3 0 103 238 179 887.3 0 179 239 216 887.3 0 216 240 146 887.3 0 146 241 164 887.3 0 164 242 102 887.3 0 102 243 132 887.3 0 132 244 119 887.3 0 119 245 154 887.3 0 154 246 141 887.3 0 141 247 143 887.3 0 143 248 161 887.3 0 161 249 119 887.3 0 119 250 86 887.3 0 86 251 95 887.3 0 95 252 113 887.3 0 113 253 101 887.3 0 101 254 112 887.3 0 112 255 135 887.3 0 135 256 390 887.3 0 390 257 433 887.3 0 433 258 541 887.3 0 541 259 182 887.3 0 182 260 139 887.3 0 139 261 114 887.3 0 114 262 98 887.3 0 98 263 90 887.3 0 90 264 106 887.3 0 106 265 90 887.3 0 90 266 254 887.3 0 254 267 383 887.3 0 383 268 530 887.3 0 530 269 206 887.3 0 206 270 170 887.3 0 170 271 195 887.3 0 195 272 633 887.3 0 633 273 693 887.3 0 693 274 546 887.3 0 546 275 308 887.3 0 308 276 206 887.3 0 206 277 210 887.3 0 210 278 201 887.3 0 201 279 136 887.3 0 136 280 241 887.3 0 241 281 186 887.3 0 186 282 232 887.3 0 232 283 168 887.3 0 168 284 139 887.3 0 139 285 147 887.3 0 147 286 161 887.3 0 161 287 318 887.3 0 318 288 265 887.3 0 265 289 253 887.3 0 253 290 114 887.3 0 114 291 105 887.3 0 105 292 128 887.3 0 128 293 182 887.3 0 182 294 186 887.3 0 186 295 99 887.3 0 99 296 66 887.3 0 66 297 96 887.3 0 96 298 121 887.3 0 121 299 126 887.3 0 126 300 136 887.3 0 136 301 173 887.3 0 173 Finished in 5.71 s (65 us/read; 0.93 M reads/minute). === Summary === Total reads processed: 88,200 Reads with adapters: 17,020 (19.3%) Reads that were too short: 33 (0.0%) Reads written (passing filters): 16,987 (19.3%) Total basepairs processed: 18,644,807 bp Total written (filtered): 4,669,558 bp (25.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17020 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 6 0.0 3 0 0 6 16 101 0.0 3 0 94 7 17 16289 0.0 3 15897 376 12 4 18 537 0.0 3 494 42 1 19 16 0.0 3 4 1 7 4 20 19 0.0 3 0 0 1 18 21 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 75 5 0.0 3 0 0 0 5 91 1 0.0 3 0 0 0 1 96 1 0.0 3 1 99 1 0.0 3 0 1 107 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 164 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 185 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 285 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.04 s (61 us/read; 0.98 M reads/minute). === Summary === Total reads processed: 16,987 Reads with adapters: 747 (4.4%) Reads written (passing filters): 16,987 (100.0%) Total basepairs processed: 4,669,558 bp Total written (filtered): 4,667,304 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 747 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 78.4% C: 5.1% G: 15.7% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 734 265.4 0 734 4 13 66.4 0 13