This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 150.83 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 614,140 (19.4%) Reads that were too short: 9,015 (0.3%) Reads written (passing filters): 605,125 (19.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 153,126,375 bp (16.1%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 614140 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2168 773.1 0 2168 7 10400 773.1 0 10400 8 10683 773.1 0 10683 9 74742 773.1 0 74742 10 184 773.1 0 184 11 228 773.1 0 228 12 156 773.1 0 156 13 23 773.1 0 23 14 31 773.1 0 31 15 55 773.1 0 55 16 74 773.1 0 74 17 86 773.1 0 86 18 33 773.1 0 33 19 66 773.1 0 66 20 55 773.1 0 55 21 102 773.1 0 102 22 242 773.1 0 242 23 87 773.1 0 87 24 164 773.1 0 164 25 149 773.1 0 149 26 151 773.1 0 151 27 453 773.1 0 453 28 125 773.1 0 125 29 242 773.1 0 242 30 213 773.1 0 213 31 253 773.1 0 253 32 161 773.1 0 161 33 363 773.1 0 363 34 424 773.1 0 424 35 844 773.1 0 844 36 676 773.1 0 676 37 1416 773.1 0 1416 38 2754 773.1 0 2754 39 41086 773.1 0 41086 40 26814 773.1 0 26814 41 64052 773.1 0 64052 42 469 773.1 0 469 43 349 773.1 0 349 44 531 773.1 0 531 45 1096 773.1 0 1096 46 2213 773.1 0 2213 47 477 773.1 0 477 48 550 773.1 0 550 49 997 773.1 0 997 50 374 773.1 0 374 51 438 773.1 0 438 52 1003 773.1 0 1003 53 811 773.1 0 811 54 1176 773.1 0 1176 55 1221 773.1 0 1221 56 1984 773.1 0 1984 57 2796 773.1 0 2796 58 3917 773.1 0 3917 59 107869 773.1 0 107869 60 82558 773.1 0 82558 61 144202 773.1 0 144202 62 1319 773.1 0 1319 63 20 773.1 0 20 64 15 773.1 0 15 65 4 773.1 0 4 66 3 773.1 0 3 67 6 773.1 0 6 68 9 773.1 0 9 69 4 773.1 0 4 70 10 773.1 0 10 71 14 773.1 0 14 72 32 773.1 0 32 73 97 773.1 0 97 74 97 773.1 0 97 75 90 773.1 0 90 76 3 773.1 0 3 77 5 773.1 0 5 78 7 773.1 0 7 79 7 773.1 0 7 80 7 773.1 0 7 81 36 773.1 0 36 82 33 773.1 0 33 83 29 773.1 0 29 84 6 773.1 0 6 85 7 773.1 0 7 86 12 773.1 0 12 87 6 773.1 0 6 88 11 773.1 0 11 89 9 773.1 0 9 90 6 773.1 0 6 91 7 773.1 0 7 92 6 773.1 0 6 93 4 773.1 0 4 94 5 773.1 0 5 95 7 773.1 0 7 96 5 773.1 0 5 97 6 773.1 0 6 98 13 773.1 0 13 99 12 773.1 0 12 100 28 773.1 0 28 101 415 773.1 0 415 102 589 773.1 0 589 103 511 773.1 0 511 104 8 773.1 0 8 105 16 773.1 0 16 106 21 773.1 0 21 107 5 773.1 0 5 108 13 773.1 0 13 109 26 773.1 0 26 110 71 773.1 0 71 111 1306 773.1 0 1306 112 311 773.1 0 311 113 545 773.1 0 545 114 10 773.1 0 10 115 2 773.1 0 2 116 3 773.1 0 3 117 1 773.1 0 1 118 11 773.1 0 11 119 14 773.1 0 14 120 6 773.1 0 6 121 4 773.1 0 4 122 5 773.1 0 5 123 11 773.1 0 11 124 4 773.1 0 4 125 31 773.1 0 31 126 20 773.1 0 20 127 8 773.1 0 8 128 26 773.1 0 26 129 10 773.1 0 10 130 20 773.1 0 20 131 42 773.1 0 42 132 14 773.1 0 14 133 4 773.1 0 4 134 7 773.1 0 7 135 8 773.1 0 8 136 10 773.1 0 10 137 15 773.1 0 15 138 13 773.1 0 13 139 16 773.1 0 16 140 11 773.1 0 11 141 25 773.1 0 25 142 15 773.1 0 15 143 20 773.1 0 20 144 13 773.1 0 13 145 13 773.1 0 13 146 37 773.1 0 37 147 17 773.1 0 17 148 26 773.1 0 26 149 30 773.1 0 30 150 16 773.1 0 16 151 9 773.1 0 9 152 10 773.1 0 10 153 5 773.1 0 5 154 5 773.1 0 5 155 10 773.1 0 10 156 15 773.1 0 15 157 56 773.1 0 56 158 13 773.1 0 13 159 9 773.1 0 9 160 12 773.1 0 12 161 17 773.1 0 17 162 56 773.1 0 56 163 36 773.1 0 36 164 21 773.1 0 21 165 10 773.1 0 10 166 14 773.1 0 14 167 45 773.1 0 45 168 24 773.1 0 24 169 62 773.1 0 62 170 92 773.1 0 92 171 104 773.1 0 104 172 14 773.1 0 14 173 14 773.1 0 14 174 12 773.1 0 12 175 6 773.1 0 6 176 7 773.1 0 7 177 20 773.1 0 20 178 38 773.1 0 38 179 22 773.1 0 22 180 13 773.1 0 13 181 21 773.1 0 21 182 19 773.1 0 19 183 80 773.1 0 80 184 321 773.1 0 321 185 126 773.1 0 126 186 206 773.1 0 206 187 82 773.1 0 82 188 43 773.1 0 43 189 40 773.1 0 40 190 22 773.1 0 22 191 10 773.1 0 10 192 8 773.1 0 8 193 15 773.1 0 15 194 13 773.1 0 13 195 33 773.1 0 33 196 50 773.1 0 50 197 252 773.1 0 252 198 671 773.1 0 671 199 392 773.1 0 392 200 646 773.1 0 646 201 146 773.1 0 146 202 115 773.1 0 115 203 303 773.1 0 303 204 209 773.1 0 209 205 526 773.1 0 526 206 43 773.1 0 43 207 23 773.1 0 23 208 22 773.1 0 22 209 22 773.1 0 22 210 24 773.1 0 24 211 16 773.1 0 16 212 22 773.1 0 22 213 17 773.1 0 17 214 27 773.1 0 27 215 21 773.1 0 21 216 13 773.1 0 13 217 9 773.1 0 9 218 74 773.1 0 74 219 79 773.1 0 79 220 105 773.1 0 105 221 43 773.1 0 43 222 24 773.1 0 24 223 320 773.1 0 320 224 424 773.1 0 424 225 400 773.1 0 400 226 386 773.1 0 386 227 445 773.1 0 445 228 1136 773.1 0 1136 229 486 773.1 0 486 230 494 773.1 0 494 231 8 773.1 0 8 232 7 773.1 0 7 233 6 773.1 0 6 234 6 773.1 0 6 235 15 773.1 0 15 236 15 773.1 0 15 237 13 773.1 0 13 238 19 773.1 0 19 239 21 773.1 0 21 240 12 773.1 0 12 241 18 773.1 0 18 242 21 773.1 0 21 243 19 773.1 0 19 244 32 773.1 0 32 245 17 773.1 0 17 246 12 773.1 0 12 247 17 773.1 0 17 248 26 773.1 0 26 249 19 773.1 0 19 250 16 773.1 0 16 251 19 773.1 0 19 252 5 773.1 0 5 253 7 773.1 0 7 254 14 773.1 0 14 255 90 773.1 0 90 256 112 773.1 0 112 257 153 773.1 0 153 258 117 773.1 0 117 259 90 773.1 0 90 260 51 773.1 0 51 261 49 773.1 0 49 262 17 773.1 0 17 263 24 773.1 0 24 264 29 773.1 0 29 265 15 773.1 0 15 266 19 773.1 0 19 267 18 773.1 0 18 268 31 773.1 0 31 269 17 773.1 0 17 270 21 773.1 0 21 271 61 773.1 0 61 272 58 773.1 0 58 273 94 773.1 0 94 274 97 773.1 0 97 275 171 773.1 0 171 276 88 773.1 0 88 277 158 773.1 0 158 278 19 773.1 0 19 279 40 773.1 0 40 280 26 773.1 0 26 281 49 773.1 0 49 282 22 773.1 0 22 283 23 773.1 0 23 284 29 773.1 0 29 285 65 773.1 0 65 286 68 773.1 0 68 287 65 773.1 0 65 288 37 773.1 0 37 289 38 773.1 0 38 290 67 773.1 0 67 291 93 773.1 0 93 292 129 773.1 0 129 293 106 773.1 0 106 294 76 773.1 0 76 295 37 773.1 0 37 296 32 773.1 0 32 297 33 773.1 0 33 298 30 773.1 0 30 299 41 773.1 0 41 300 44 773.1 0 44 301 74 773.1 0 74 Finished in 39.91 s (66 us/read; 0.91 M reads/minute). === Summary === Total reads processed: 605,125 Reads with adapters: 26,908 (4.4%) Reads that were too short: 332 (0.1%) Reads written (passing filters): 26,576 (4.4%) Total basepairs processed: 153,126,375 bp Total written (filtered): 5,540,525 bp (3.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26908 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 3 0.0 3 0 0 2 0 1 19 57 0.0 3 0 42 10 4 1 20 9807 0.0 4 9337 364 73 22 11 21 34 0.0 4 5 28 1 22 18 0.0 4 4 13 0 1 23 562 0.0 4 512 15 3 20 12 24 2 0.0 4 1 0 0 0 1 26 2 0.0 4 0 0 0 2 33 1 0.0 4 0 0 0 1 39 2 0.0 4 1 0 0 0 1 40 6 0.0 4 0 0 0 0 6 41 2 0.0 4 0 0 0 0 2 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 51 1 0.0 4 1 72 2 0.0 4 0 0 0 0 2 73 22 0.0 4 0 0 0 0 22 74 12 0.0 4 0 0 0 0 12 75 14987 0.0 4 0 0 0 0 14987 77 8 0.0 4 0 0 0 1 7 78 26 0.0 4 0 0 0 0 26 81 1 0.0 4 0 0 0 0 1 83 8 0.0 4 0 0 0 0 8 88 2 0.0 4 1 0 0 0 1 95 1 0.0 4 0 0 0 0 1 101 2 0.0 4 0 0 0 0 2 105 1 0.0 4 1 122 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 1 125 1 0.0 4 0 0 0 0 1 126 68 0.0 4 0 0 0 0 68 127 916 0.0 4 0 0 0 0 916 128 3 0.0 4 0 0 0 0 3 129 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 131 1 0.0 4 0 0 0 0 1 137 2 0.0 4 0 0 0 1 1 139 2 0.0 4 0 0 0 0 2 144 1 0.0 4 0 0 0 0 1 151 4 0.0 4 0 0 0 0 4 152 2 0.0 4 0 0 0 0 2 155 3 0.0 4 0 0 0 0 3 156 3 0.0 4 1 0 0 0 2 157 3 0.0 4 0 0 0 0 3 158 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 169 2 0.0 4 0 0 0 0 2 170 2 0.0 4 0 0 0 0 2 173 10 0.0 4 0 0 0 0 10 174 3 0.0 4 0 0 0 1 2 176 75 0.0 4 0 0 0 3 72 177 34 0.0 4 0 0 0 1 33 178 4 0.0 4 0 0 0 0 4 180 2 0.0 4 0 0 0 0 2 181 7 0.0 4 0 0 0 0 7 182 15 0.0 4 0 0 0 1 14 183 16 0.0 4 0 0 0 0 16 184 20 0.0 4 0 0 0 0 20 185 1 0.0 4 0 0 0 0 1 186 2 0.0 4 0 0 0 0 2 202 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 205 8 0.0 4 0 0 1 0 7 208 1 0.0 4 0 0 0 0 1 211 2 0.0 4 0 0 0 0 2 212 3 0.0 4 0 0 0 0 3 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 1 215 2 0.0 4 0 0 0 0 2 218 2 0.0 4 0 0 0 0 2 222 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 0 1 227 5 0.0 4 0 0 0 0 5 228 7 0.0 4 0 0 0 0 7 229 5 0.0 4 0 0 0 0 5 230 42 0.0 4 0 0 0 0 42 231 9 0.0 4 0 0 0 1 8 232 3 0.0 4 0 0 0 0 3 233 8 0.0 4 0 0 0 0 8 234 1 0.0 4 0 0 0 1 236 3 0.0 4 0 0 0 0 3 237 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 241 2 0.0 4 0 0 0 0 2 242 1 0.0 4 0 0 0 0 1 244 5 0.0 4 0 0 0 0 5 263 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 284 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.33 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 26,576 Reads with adapters: 2,511 (9.4%) Reads written (passing filters): 26,576 (100.0%) Total basepairs processed: 5,540,525 bp Total written (filtered): 5,512,720 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2511 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.3% C: 4.6% G: 85.8% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 59 415.2 0 59 4 110 103.8 0 110 5 1 26.0 0 1 7 1 1.6 0 1 8 1 0.4 0 1 9 52 0.1 0 2 50 10 195 0.0 1 7 188 11 2076 0.0 1 36 2040 12 5 0.0 1 0 5 13 2 0.0 1 0 2 14 1 0.0 1 0 1 209 4 0.0 1 3 1 224 2 0.0 1 2 265 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.