This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGATCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 151.70 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 635,421 (20.1%) Reads that were too short: 4,825 (0.2%) Reads written (passing filters): 630,596 (19.9%) Total basepairs processed: 953,096,634 bp Total written (filtered): 159,830,257 bp (16.8%) === Adapter 1 === Sequence: TGATCC; Type: regular 5'; Length: 6; Trimmed: 635421 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1357 773.1 0 1357 7 9609 773.1 0 9609 8 12336 773.1 0 12336 9 54110 773.1 0 54110 10 121 773.1 0 121 11 104 773.1 0 104 12 207 773.1 0 207 13 19 773.1 0 19 14 27 773.1 0 27 15 65 773.1 0 65 16 55 773.1 0 55 17 93 773.1 0 93 18 55 773.1 0 55 19 70 773.1 0 70 20 43 773.1 0 43 21 139 773.1 0 139 22 339 773.1 0 339 23 90 773.1 0 90 24 194 773.1 0 194 25 185 773.1 0 185 26 200 773.1 0 200 27 691 773.1 0 691 28 179 773.1 0 179 29 213 773.1 0 213 30 128 773.1 0 128 31 171 773.1 0 171 32 229 773.1 0 229 33 374 773.1 0 374 34 522 773.1 0 522 35 1017 773.1 0 1017 36 834 773.1 0 834 37 1879 773.1 0 1879 38 3476 773.1 0 3476 39 62444 773.1 0 62444 40 38687 773.1 0 38687 41 96791 773.1 0 96791 42 581 773.1 0 581 43 373 773.1 0 373 44 558 773.1 0 558 45 1030 773.1 0 1030 46 2216 773.1 0 2216 47 470 773.1 0 470 48 478 773.1 0 478 49 995 773.1 0 995 50 358 773.1 0 358 51 424 773.1 0 424 52 940 773.1 0 940 53 752 773.1 0 752 54 943 773.1 0 943 55 1005 773.1 0 1005 56 1670 773.1 0 1670 57 2376 773.1 0 2376 58 2815 773.1 0 2815 59 100415 773.1 0 100415 60 79264 773.1 0 79264 61 138554 773.1 0 138554 62 1158 773.1 0 1158 63 22 773.1 0 22 64 8 773.1 0 8 65 5 773.1 0 5 66 3 773.1 0 3 68 3 773.1 0 3 69 3 773.1 0 3 70 4 773.1 0 4 71 10 773.1 0 10 72 24 773.1 0 24 73 120 773.1 0 120 74 79 773.1 0 79 75 132 773.1 0 132 76 4 773.1 0 4 77 3 773.1 0 3 78 1 773.1 0 1 79 1 773.1 0 1 80 9 773.1 0 9 81 16 773.1 0 16 82 12 773.1 0 12 83 5 773.1 0 5 84 4 773.1 0 4 85 2 773.1 0 2 86 1 773.1 0 1 87 3 773.1 0 3 88 4 773.1 0 4 89 5 773.1 0 5 90 5 773.1 0 5 91 3 773.1 0 3 92 2 773.1 0 2 93 2 773.1 0 2 94 2 773.1 0 2 95 5 773.1 0 5 96 3 773.1 0 3 97 8 773.1 0 8 98 8 773.1 0 8 99 5 773.1 0 5 100 21 773.1 0 21 101 194 773.1 0 194 102 300 773.1 0 300 103 342 773.1 0 342 104 8 773.1 0 8 105 9 773.1 0 9 106 12 773.1 0 12 107 6 773.1 0 6 108 10 773.1 0 10 109 9 773.1 0 9 110 43 773.1 0 43 111 1137 773.1 0 1137 112 262 773.1 0 262 113 509 773.1 0 509 114 6 773.1 0 6 115 2 773.1 0 2 116 3 773.1 0 3 117 7 773.1 0 7 118 7 773.1 0 7 119 6 773.1 0 6 120 2 773.1 0 2 121 3 773.1 0 3 122 6 773.1 0 6 123 3 773.1 0 3 124 4 773.1 0 4 125 27 773.1 0 27 126 19 773.1 0 19 127 11 773.1 0 11 128 24 773.1 0 24 129 7 773.1 0 7 130 16 773.1 0 16 131 33 773.1 0 33 132 7 773.1 0 7 133 4 773.1 0 4 134 6 773.1 0 6 135 4 773.1 0 4 136 5 773.1 0 5 137 8 773.1 0 8 138 5 773.1 0 5 139 8 773.1 0 8 140 3 773.1 0 3 141 20 773.1 0 20 142 6 773.1 0 6 143 14 773.1 0 14 144 4 773.1 0 4 145 10 773.1 0 10 146 42 773.1 0 42 147 16 773.1 0 16 148 34 773.1 0 34 149 18 773.1 0 18 150 18 773.1 0 18 151 2 773.1 0 2 152 6 773.1 0 6 153 7 773.1 0 7 154 4 773.1 0 4 155 6 773.1 0 6 156 15 773.1 0 15 157 55 773.1 0 55 158 11 773.1 0 11 159 7 773.1 0 7 160 8 773.1 0 8 161 7 773.1 0 7 162 33 773.1 0 33 163 14 773.1 0 14 164 12 773.1 0 12 165 1 773.1 0 1 166 17 773.1 0 17 167 46 773.1 0 46 168 13 773.1 0 13 169 79 773.1 0 79 170 102 773.1 0 102 171 81 773.1 0 81 172 4 773.1 0 4 173 9 773.1 0 9 174 5 773.1 0 5 175 3 773.1 0 3 176 3 773.1 0 3 177 10 773.1 0 10 178 16 773.1 0 16 179 10 773.1 0 10 180 6 773.1 0 6 181 13 773.1 0 13 182 16 773.1 0 16 183 54 773.1 0 54 184 357 773.1 0 357 185 57 773.1 0 57 186 213 773.1 0 213 187 67 773.1 0 67 188 22 773.1 0 22 189 21 773.1 0 21 190 5 773.1 0 5 191 10 773.1 0 10 192 6 773.1 0 6 193 11 773.1 0 11 194 15 773.1 0 15 195 10 773.1 0 10 196 28 773.1 0 28 197 126 773.1 0 126 198 272 773.1 0 272 199 165 773.1 0 165 200 259 773.1 0 259 201 43 773.1 0 43 202 27 773.1 0 27 203 60 773.1 0 60 204 28 773.1 0 28 205 75 773.1 0 75 206 14 773.1 0 14 207 10 773.1 0 10 208 6 773.1 0 6 209 6 773.1 0 6 210 3 773.1 0 3 211 8 773.1 0 8 212 7 773.1 0 7 213 9 773.1 0 9 214 11 773.1 0 11 215 10 773.1 0 10 216 10 773.1 0 10 217 7 773.1 0 7 218 26 773.1 0 26 219 19 773.1 0 19 220 33 773.1 0 33 221 25 773.1 0 25 222 21 773.1 0 21 223 75 773.1 0 75 224 114 773.1 0 114 225 111 773.1 0 111 226 373 773.1 0 373 227 451 773.1 0 451 228 1056 773.1 0 1056 229 441 773.1 0 441 230 245 773.1 0 245 231 4 773.1 0 4 232 5 773.1 0 5 233 3 773.1 0 3 234 5 773.1 0 5 235 12 773.1 0 12 236 11 773.1 0 11 237 5 773.1 0 5 238 14 773.1 0 14 239 8 773.1 0 8 240 4 773.1 0 4 241 13 773.1 0 13 242 5 773.1 0 5 243 17 773.1 0 17 244 6 773.1 0 6 245 10 773.1 0 10 246 5 773.1 0 5 247 7 773.1 0 7 248 11 773.1 0 11 249 11 773.1 0 11 250 11 773.1 0 11 251 6 773.1 0 6 252 3 773.1 0 3 253 7 773.1 0 7 254 7 773.1 0 7 255 37 773.1 0 37 256 47 773.1 0 47 257 79 773.1 0 79 258 40 773.1 0 40 259 33 773.1 0 33 260 16 773.1 0 16 261 13 773.1 0 13 262 8 773.1 0 8 263 12 773.1 0 12 264 15 773.1 0 15 265 15 773.1 0 15 266 8 773.1 0 8 267 11 773.1 0 11 268 11 773.1 0 11 269 6 773.1 0 6 270 13 773.1 0 13 271 24 773.1 0 24 272 34 773.1 0 34 273 60 773.1 0 60 274 53 773.1 0 53 275 146 773.1 0 146 276 54 773.1 0 54 277 130 773.1 0 130 278 8 773.1 0 8 279 17 773.1 0 17 280 18 773.1 0 18 281 16 773.1 0 16 282 5 773.1 0 5 283 11 773.1 0 11 284 7 773.1 0 7 285 32 773.1 0 32 286 30 773.1 0 30 287 34 773.1 0 34 288 15 773.1 0 15 289 14 773.1 0 14 290 22 773.1 0 22 291 34 773.1 0 34 292 66 773.1 0 66 293 31 773.1 0 31 294 30 773.1 0 30 295 11 773.1 0 11 296 10 773.1 0 10 297 12 773.1 0 12 298 15 773.1 0 15 299 16 773.1 0 16 300 13 773.1 0 13 301 22 773.1 0 22 Finished in 36.59 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 630,596 Reads with adapters: 10,606 (1.7%) Reads that were too short: 205 (0.0%) Reads written (passing filters): 10,401 (1.6%) Total basepairs processed: 159,830,257 bp Total written (filtered): 2,734,191 bp (1.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10606 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 33 0.0 3 0 32 1 17 9420 0.0 3 9238 174 7 1 18 25 0.0 3 3 22 19 4 0.0 3 1 0 3 20 10 0.0 3 0 0 0 10 24 3 0.0 3 0 0 0 3 29 1 0.0 3 0 0 0 1 31 1 0.0 3 1 41 4 0.0 3 0 0 0 4 48 3 0.0 3 3 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 74 1 0.0 3 1 81 18 0.0 3 0 0 0 18 82 1 0.0 3 0 0 0 1 84 5 0.0 3 1 0 0 4 90 2 0.0 3 0 0 0 2 92 3 0.0 3 0 0 0 3 99 1 0.0 3 1 102 1 0.0 3 0 1 105 1 0.0 3 0 0 0 1 107 6 0.0 3 0 0 0 6 108 2 0.0 3 0 0 0 2 111 2 0.0 3 0 0 0 2 112 19 0.0 3 0 0 0 19 114 6 0.0 3 1 0 0 5 115 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 8 0.0 3 0 0 0 8 128 5 0.0 3 0 0 0 5 130 3 0.0 3 0 0 0 3 131 9 0.0 3 0 0 0 9 132 408 0.0 3 0 0 1 407 133 15 0.0 3 0 0 0 15 134 1 0.0 3 0 0 0 1 135 47 0.0 3 0 0 2 45 136 9 0.0 3 0 0 0 9 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 17 0.0 3 0 0 0 17 159 4 0.0 3 0 0 0 4 160 23 0.0 3 0 0 0 23 161 2 0.0 3 0 0 0 2 162 1 0.0 3 0 0 0 1 163 104 0.0 3 0 0 0 104 164 8 0.0 3 0 0 0 8 165 1 0.0 3 0 0 0 1 166 12 0.0 3 0 0 0 12 167 8 0.0 3 0 0 1 7 168 1 0.0 3 0 0 0 1 173 1 0.0 3 1 174 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 177 16 0.0 3 0 0 0 16 178 4 0.0 3 0 0 0 4 180 4 0.0 3 0 0 0 4 181 1 0.0 3 0 0 0 1 182 5 0.0 3 0 0 0 5 183 51 0.0 3 0 0 1 50 184 66 0.0 3 0 0 0 66 185 6 0.0 3 0 0 0 6 186 8 0.0 3 0 0 0 8 187 2 0.0 3 0 0 0 2 189 4 0.0 3 0 0 0 4 190 4 0.0 3 0 0 0 4 192 3 0.0 3 0 0 0 3 195 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 211 2 0.0 3 0 0 0 2 214 1 0.0 3 0 0 0 1 218 3 0.0 3 0 0 0 3 220 3 0.0 3 0 0 0 3 223 5 0.0 3 0 0 0 5 224 3 0.0 3 0 0 0 3 232 1 0.0 3 0 0 0 1 233 13 0.0 3 0 0 0 13 234 56 0.0 3 0 0 2 54 235 3 0.0 3 0 0 0 3 238 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 242 3 0.0 3 0 0 0 3 243 3 0.0 3 0 0 0 3 244 1 0.0 3 0 0 0 1 247 6 0.0 3 0 0 0 6 249 4 0.0 3 0 0 0 4 250 2 0.0 3 0 0 0 2 260 2 0.0 3 0 0 1 1 266 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 1 275 2 0.0 3 0 0 0 2 276 5 0.0 3 0 0 1 4 277 4 0.0 3 0 0 0 4 278 3 0.0 3 0 0 0 3 279 5 0.0 3 0 1 0 4 280 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 285 4 0.0 3 0 0 1 3 288 1 0.0 3 0 0 0 1 289 1 0.0 3 0 0 0 1 292 5 0.0 3 0 0 0 5 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.54 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 10,401 Reads with adapters: 14 (0.1%) Reads written (passing filters): 10,401 (100.0%) Total basepairs processed: 2,734,191 bp Total written (filtered): 2,734,146 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 14 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.7% C: 28.6% G: 14.3% T: 21.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 11 162.5 0 11 4 3 40.6 0 3