This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 139.74 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 164,335 (5.2%) Reads that were too short: 31,125 (1.0%) Reads written (passing filters): 133,210 (4.2%) Total basepairs processed: 953,096,634 bp Total written (filtered): 37,317,129 bp (3.9%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 164335 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3607 773.1 0 3607 7 53740 773.1 0 53740 8 892 773.1 0 892 9 57998 773.1 0 57998 10 62 773.1 0 62 11 110 773.1 0 110 12 53 773.1 0 53 13 5 773.1 0 5 14 4 773.1 0 4 15 4 773.1 0 4 16 3 773.1 0 3 17 22 773.1 0 22 18 6 773.1 0 6 19 5 773.1 0 5 20 5 773.1 0 5 21 3 773.1 0 3 22 11 773.1 0 11 23 6 773.1 0 6 24 12 773.1 0 12 25 10 773.1 0 10 26 12 773.1 0 12 27 55 773.1 0 55 28 383 773.1 0 383 29 72 773.1 0 72 30 10 773.1 0 10 31 10 773.1 0 10 32 9 773.1 0 9 33 9 773.1 0 9 34 21 773.1 0 21 35 10 773.1 0 10 36 9 773.1 0 9 37 25 773.1 0 25 38 12 773.1 0 12 39 15 773.1 0 15 40 4 773.1 0 4 41 10 773.1 0 10 42 19 773.1 0 19 43 15 773.1 0 15 44 10 773.1 0 10 45 29 773.1 0 29 46 28 773.1 0 28 47 32 773.1 0 32 48 154 773.1 0 154 49 8 773.1 0 8 50 46 773.1 0 46 51 213 773.1 0 213 52 35 773.1 0 35 53 29 773.1 0 29 54 28 773.1 0 28 55 41 773.1 0 41 56 20 773.1 0 20 57 8 773.1 0 8 58 21 773.1 0 21 59 59 773.1 0 59 60 38 773.1 0 38 61 144 773.1 0 144 62 556 773.1 0 556 63 524 773.1 0 524 64 486 773.1 0 486 65 68 773.1 0 68 66 56 773.1 0 56 67 29 773.1 0 29 68 48 773.1 0 48 69 41 773.1 0 41 70 16 773.1 0 16 71 18 773.1 0 18 72 56 773.1 0 56 73 84 773.1 0 84 74 53 773.1 0 53 75 66 773.1 0 66 76 68 773.1 0 68 77 101 773.1 0 101 78 1214 773.1 0 1214 79 979 773.1 0 979 80 1416 773.1 0 1416 81 353 773.1 0 353 82 115 773.1 0 115 83 831 773.1 0 831 84 36 773.1 0 36 85 22 773.1 0 22 86 160 773.1 0 160 87 16 773.1 0 16 88 11 773.1 0 11 89 18 773.1 0 18 90 22 773.1 0 22 91 22 773.1 0 22 92 29 773.1 0 29 93 31 773.1 0 31 94 26 773.1 0 26 95 22 773.1 0 22 96 18 773.1 0 18 97 16 773.1 0 16 98 10 773.1 0 10 99 15 773.1 0 15 100 27 773.1 0 27 101 29 773.1 0 29 102 124 773.1 0 124 103 20 773.1 0 20 104 8 773.1 0 8 105 19 773.1 0 19 106 8 773.1 0 8 107 18 773.1 0 18 108 38 773.1 0 38 109 115 773.1 0 115 110 92 773.1 0 92 111 94 773.1 0 94 112 21 773.1 0 21 113 20 773.1 0 20 114 23 773.1 0 23 115 73 773.1 0 73 116 131 773.1 0 131 117 120 773.1 0 120 118 40 773.1 0 40 119 35 773.1 0 35 120 26 773.1 0 26 121 52 773.1 0 52 122 48 773.1 0 48 123 57 773.1 0 57 124 62 773.1 0 62 125 92 773.1 0 92 126 94 773.1 0 94 127 52 773.1 0 52 128 42 773.1 0 42 129 43 773.1 0 43 130 66 773.1 0 66 131 58 773.1 0 58 132 35 773.1 0 35 133 28 773.1 0 28 134 12 773.1 0 12 135 20 773.1 0 20 136 20 773.1 0 20 137 30 773.1 0 30 138 34 773.1 0 34 139 18 773.1 0 18 140 22 773.1 0 22 141 79 773.1 0 79 142 87 773.1 0 87 143 44 773.1 0 44 144 60 773.1 0 60 145 54 773.1 0 54 146 92 773.1 0 92 147 26 773.1 0 26 148 47 773.1 0 47 149 20 773.1 0 20 150 82 773.1 0 82 151 35 773.1 0 35 152 33 773.1 0 33 153 26 773.1 0 26 154 39 773.1 0 39 155 33 773.1 0 33 156 27 773.1 0 27 157 83 773.1 0 83 158 31 773.1 0 31 159 30 773.1 0 30 160 44 773.1 0 44 161 27 773.1 0 27 162 36 773.1 0 36 163 28 773.1 0 28 164 38 773.1 0 38 165 66 773.1 0 66 166 71 773.1 0 71 167 80 773.1 0 80 168 46 773.1 0 46 169 25 773.1 0 25 170 36 773.1 0 36 171 55 773.1 0 55 172 55 773.1 0 55 173 34 773.1 0 34 174 35 773.1 0 35 175 36 773.1 0 36 176 49 773.1 0 49 177 43 773.1 0 43 178 41 773.1 0 41 179 62 773.1 0 62 180 100 773.1 0 100 181 394 773.1 0 394 182 343 773.1 0 343 183 423 773.1 0 423 184 110 773.1 0 110 185 82 773.1 0 82 186 335 773.1 0 335 187 234 773.1 0 234 188 156 773.1 0 156 189 31 773.1 0 31 190 201 773.1 0 201 191 187 773.1 0 187 192 245 773.1 0 245 193 137 773.1 0 137 194 77 773.1 0 77 195 47 773.1 0 47 196 92 773.1 0 92 197 79 773.1 0 79 198 117 773.1 0 117 199 89 773.1 0 89 200 41 773.1 0 41 201 36 773.1 0 36 202 38 773.1 0 38 203 62 773.1 0 62 204 126 773.1 0 126 205 107 773.1 0 107 206 79 773.1 0 79 207 36 773.1 0 36 208 107 773.1 0 107 209 138 773.1 0 138 210 328 773.1 0 328 211 186 773.1 0 186 212 99 773.1 0 99 213 89 773.1 0 89 214 87 773.1 0 87 215 115 773.1 0 115 216 109 773.1 0 109 217 573 773.1 0 573 218 638 773.1 0 638 219 632 773.1 0 632 220 126 773.1 0 126 221 128 773.1 0 128 222 198 773.1 0 198 223 177 773.1 0 177 224 206 773.1 0 206 225 3084 773.1 0 3084 226 2662 773.1 0 2662 227 5081 773.1 0 5081 228 561 773.1 0 561 229 206 773.1 0 206 230 82 773.1 0 82 231 67 773.1 0 67 232 89 773.1 0 89 233 106 773.1 0 106 234 73 773.1 0 73 235 125 773.1 0 125 236 138 773.1 0 138 237 609 773.1 0 609 238 635 773.1 0 635 239 846 773.1 0 846 240 393 773.1 0 393 241 232 773.1 0 232 242 226 773.1 0 226 243 227 773.1 0 227 244 155 773.1 0 155 245 264 773.1 0 264 246 354 773.1 0 354 247 302 773.1 0 302 248 205 773.1 0 205 249 124 773.1 0 124 250 122 773.1 0 122 251 69 773.1 0 69 252 52 773.1 0 52 253 56 773.1 0 56 254 70 773.1 0 70 255 92 773.1 0 92 256 84 773.1 0 84 257 82 773.1 0 82 258 66 773.1 0 66 259 84 773.1 0 84 260 591 773.1 0 591 261 388 773.1 0 388 262 656 773.1 0 656 263 206 773.1 0 206 264 221 773.1 0 221 265 108 773.1 0 108 266 117 773.1 0 117 267 194 773.1 0 194 268 85 773.1 0 85 269 69 773.1 0 69 270 55 773.1 0 55 271 71 773.1 0 71 272 179 773.1 0 179 273 164 773.1 0 164 274 163 773.1 0 163 275 98 773.1 0 98 276 75 773.1 0 75 277 73 773.1 0 73 278 121 773.1 0 121 279 159 773.1 0 159 280 218 773.1 0 218 281 164 773.1 0 164 282 151 773.1 0 151 283 84 773.1 0 84 284 80 773.1 0 80 285 274 773.1 0 274 286 557 773.1 0 557 287 710 773.1 0 710 288 711 773.1 0 711 289 98 773.1 0 98 290 116 773.1 0 116 291 151 773.1 0 151 292 125 773.1 0 125 293 140 773.1 0 140 294 139 773.1 0 139 295 133 773.1 0 133 296 152 773.1 0 152 297 146 773.1 0 146 298 121 773.1 0 121 299 287 773.1 0 287 300 257 773.1 0 257 301 511 773.1 0 511 Finished in 9.79 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 133,210 Reads with adapters: 57,270 (43.0%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 57,236 (43.0%) Total basepairs processed: 37,317,129 bp Total written (filtered): 15,582,401 bp (41.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 57270 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 4 0.0 3 0 0 3 1 19 274 0.0 3 0 205 39 22 8 20 55779 0.0 4 53614 1817 265 66 17 21 132 0.0 4 21 105 4 0 2 22 10 0.0 4 0 2 5 1 2 23 88 0.0 4 24 0 0 52 12 26 1 0.0 4 1 28 2 0.0 4 0 0 0 0 2 31 1 0.0 4 1 38 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 1 78 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 1 113 1 0.0 4 1 125 2 0.0 4 0 0 0 0 2 126 7 0.0 4 0 0 0 0 7 127 922 0.0 4 0 0 0 0 922 128 5 0.0 4 0 0 0 0 5 130 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 1 138 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 6 0.0 4 0 0 0 0 6 233 2 0.0 4 0 0 0 1 1 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 1 1 277 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.06 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 57,236 Reads with adapters: 2,444 (4.3%) Reads written (passing filters): 57,236 (100.0%) Total basepairs processed: 15,582,401 bp Total written (filtered): 15,548,177 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2444 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.3% C: 9.2% G: 80.4% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 192 894.3 0 192 4 77 223.6 0 77 7 5 3.5 0 5 8 2 0.9 0 2 9 12 0.2 0 0 12 10 76 0.1 1 2 74 11 1503 0.0 1 27 1476 12 500 0.0 1 10 490 13 23 0.0 1 2 21 14 1 0.0 1 0 1 15 5 0.0 1 0 5 20 2 0.0 1 2 64 1 0.0 1 1 152 2 0.0 1 1 1 153 1 0.0 1 1 162 1 0.0 1 1 210 28 0.0 1 27 1 211 2 0.0 1 2 225 11 0.0 1 10 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.