This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CAGAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 142.46 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 144,334 (4.6%) Reads that were too short: 13,915 (0.4%) Reads written (passing filters): 130,419 (4.1%) Total basepairs processed: 953,096,634 bp Total written (filtered): 37,126,234 bp (3.9%) === Adapter 1 === Sequence: CAGAGC; Type: regular 5'; Length: 6; Trimmed: 144334 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1511 773.1 0 1511 7 62725 773.1 0 62725 8 579 773.1 0 579 9 54715 773.1 0 54715 10 40 773.1 0 40 11 25 773.1 0 25 12 7 773.1 0 7 13 3 773.1 0 3 14 6 773.1 0 6 15 6 773.1 0 6 16 7 773.1 0 7 17 4 773.1 0 4 18 4 773.1 0 4 19 5 773.1 0 5 20 4 773.1 0 4 21 1 773.1 0 1 22 3 773.1 0 3 23 5 773.1 0 5 24 5 773.1 0 5 25 2 773.1 0 2 26 9 773.1 0 9 27 53 773.1 0 53 28 344 773.1 0 344 29 46 773.1 0 46 30 2 773.1 0 2 31 9 773.1 0 9 32 5 773.1 0 5 33 11 773.1 0 11 34 13 773.1 0 13 35 3 773.1 0 3 36 11 773.1 0 11 37 20 773.1 0 20 38 13 773.1 0 13 39 13 773.1 0 13 40 7 773.1 0 7 41 13 773.1 0 13 42 13 773.1 0 13 43 16 773.1 0 16 44 12 773.1 0 12 45 19 773.1 0 19 46 14 773.1 0 14 47 24 773.1 0 24 48 147 773.1 0 147 49 7 773.1 0 7 50 40 773.1 0 40 51 246 773.1 0 246 52 44 773.1 0 44 53 15 773.1 0 15 54 15 773.1 0 15 55 15 773.1 0 15 56 14 773.1 0 14 57 5 773.1 0 5 58 8 773.1 0 8 59 29 773.1 0 29 60 21 773.1 0 21 61 53 773.1 0 53 62 274 773.1 0 274 63 231 773.1 0 231 64 271 773.1 0 271 65 42 773.1 0 42 66 25 773.1 0 25 67 22 773.1 0 22 68 21 773.1 0 21 69 25 773.1 0 25 70 11 773.1 0 11 71 10 773.1 0 10 72 9 773.1 0 9 73 26 773.1 0 26 74 29 773.1 0 29 75 30 773.1 0 30 76 32 773.1 0 32 77 46 773.1 0 46 78 620 773.1 0 620 79 691 773.1 0 691 80 637 773.1 0 637 81 349 773.1 0 349 82 88 773.1 0 88 83 804 773.1 0 804 84 23 773.1 0 23 85 12 773.1 0 12 86 159 773.1 0 159 87 23 773.1 0 23 88 8 773.1 0 8 89 12 773.1 0 12 90 19 773.1 0 19 91 25 773.1 0 25 92 19 773.1 0 19 93 32 773.1 0 32 94 32 773.1 0 32 95 17 773.1 0 17 96 17 773.1 0 17 97 15 773.1 0 15 98 12 773.1 0 12 99 20 773.1 0 20 100 31 773.1 0 31 101 23 773.1 0 23 102 148 773.1 0 148 103 18 773.1 0 18 104 10 773.1 0 10 105 9 773.1 0 9 106 12 773.1 0 12 107 16 773.1 0 16 108 26 773.1 0 26 109 57 773.1 0 57 110 50 773.1 0 50 111 46 773.1 0 46 112 8 773.1 0 8 113 14 773.1 0 14 114 14 773.1 0 14 115 17 773.1 0 17 116 87 773.1 0 87 117 52 773.1 0 52 118 29 773.1 0 29 119 19 773.1 0 19 120 20 773.1 0 20 121 23 773.1 0 23 122 18 773.1 0 18 123 27 773.1 0 27 124 30 773.1 0 30 125 50 773.1 0 50 126 78 773.1 0 78 127 30 773.1 0 30 128 29 773.1 0 29 129 20 773.1 0 20 130 51 773.1 0 51 131 22 773.1 0 22 132 22 773.1 0 22 133 16 773.1 0 16 134 22 773.1 0 22 135 13 773.1 0 13 136 14 773.1 0 14 137 18 773.1 0 18 138 15 773.1 0 15 139 8 773.1 0 8 140 22 773.1 0 22 141 46 773.1 0 46 142 66 773.1 0 66 143 31 773.1 0 31 144 27 773.1 0 27 145 38 773.1 0 38 146 38 773.1 0 38 147 12 773.1 0 12 148 41 773.1 0 41 149 16 773.1 0 16 150 39 773.1 0 39 151 19 773.1 0 19 152 11 773.1 0 11 153 21 773.1 0 21 154 16 773.1 0 16 155 19 773.1 0 19 156 30 773.1 0 30 157 79 773.1 0 79 158 27 773.1 0 27 159 24 773.1 0 24 160 14 773.1 0 14 161 23 773.1 0 23 162 13 773.1 0 13 163 15 773.1 0 15 164 18 773.1 0 18 165 29 773.1 0 29 166 28 773.1 0 28 167 34 773.1 0 34 168 20 773.1 0 20 169 20 773.1 0 20 170 24 773.1 0 24 171 20 773.1 0 20 172 13 773.1 0 13 173 14 773.1 0 14 174 13 773.1 0 13 175 18 773.1 0 18 176 13 773.1 0 13 177 24 773.1 0 24 178 31 773.1 0 31 179 36 773.1 0 36 180 80 773.1 0 80 181 456 773.1 0 456 182 408 773.1 0 408 183 491 773.1 0 491 184 89 773.1 0 89 185 29 773.1 0 29 186 124 773.1 0 124 187 88 773.1 0 88 188 80 773.1 0 80 189 25 773.1 0 25 190 116 773.1 0 116 191 99 773.1 0 99 192 122 773.1 0 122 193 44 773.1 0 44 194 25 773.1 0 25 195 24 773.1 0 24 196 24 773.1 0 24 197 25 773.1 0 25 198 31 773.1 0 31 199 37 773.1 0 37 200 13 773.1 0 13 201 11 773.1 0 11 202 22 773.1 0 22 203 32 773.1 0 32 204 47 773.1 0 47 205 35 773.1 0 35 206 38 773.1 0 38 207 10 773.1 0 10 208 43 773.1 0 43 209 39 773.1 0 39 210 64 773.1 0 64 211 61 773.1 0 61 212 42 773.1 0 42 213 44 773.1 0 44 214 49 773.1 0 49 215 131 773.1 0 131 216 37 773.1 0 37 217 305 773.1 0 305 218 340 773.1 0 340 219 325 773.1 0 325 220 48 773.1 0 48 221 51 773.1 0 51 222 48 773.1 0 48 223 64 773.1 0 64 224 78 773.1 0 78 225 1153 773.1 0 1153 226 915 773.1 0 915 227 1838 773.1 0 1838 228 273 773.1 0 273 229 87 773.1 0 87 230 41 773.1 0 41 231 33 773.1 0 33 232 51 773.1 0 51 233 48 773.1 0 48 234 48 773.1 0 48 235 65 773.1 0 65 236 73 773.1 0 73 237 270 773.1 0 270 238 306 773.1 0 306 239 369 773.1 0 369 240 178 773.1 0 178 241 127 773.1 0 127 242 94 773.1 0 94 243 100 773.1 0 100 244 85 773.1 0 85 245 99 773.1 0 99 246 148 773.1 0 148 247 84 773.1 0 84 248 71 773.1 0 71 249 39 773.1 0 39 250 42 773.1 0 42 251 26 773.1 0 26 252 33 773.1 0 33 253 35 773.1 0 35 254 28 773.1 0 28 255 37 773.1 0 37 256 35 773.1 0 35 257 32 773.1 0 32 258 42 773.1 0 42 259 47 773.1 0 47 260 520 773.1 0 520 261 315 773.1 0 315 262 566 773.1 0 566 263 125 773.1 0 125 264 144 773.1 0 144 265 48 773.1 0 48 266 77 773.1 0 77 267 63 773.1 0 63 268 52 773.1 0 52 269 47 773.1 0 47 270 35 773.1 0 35 271 46 773.1 0 46 272 116 773.1 0 116 273 85 773.1 0 85 274 110 773.1 0 110 275 46 773.1 0 46 276 34 773.1 0 34 277 30 773.1 0 30 278 58 773.1 0 58 279 59 773.1 0 59 280 100 773.1 0 100 281 104 773.1 0 104 282 80 773.1 0 80 283 38 773.1 0 38 284 42 773.1 0 42 285 126 773.1 0 126 286 289 773.1 0 289 287 300 773.1 0 300 288 369 773.1 0 369 289 41 773.1 0 41 290 49 773.1 0 49 291 63 773.1 0 63 292 69 773.1 0 69 293 60 773.1 0 60 294 77 773.1 0 77 295 75 773.1 0 75 296 72 773.1 0 72 297 53 773.1 0 53 298 63 773.1 0 63 299 91 773.1 0 91 300 73 773.1 0 73 301 200 773.1 0 200 Finished in 9.12 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 130,419 Reads with adapters: 56,121 (43.0%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 56,085 (43.0%) Total basepairs processed: 37,126,234 bp Total written (filtered): 15,415,397 bp (41.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 56121 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 198 0.0 3 0 194 4 17 55538 0.0 3 54501 1002 34 1 18 127 0.0 3 6 116 5 19 23 0.0 3 1 0 18 4 20 31 0.0 3 2 0 0 29 22 131 0.0 3 0 0 0 131 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 1 34 1 0.0 3 0 0 0 1 38 3 0.0 3 1 0 0 2 39 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 71 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 1 101 6 0.0 3 0 0 2 4 108 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 127 1 0.0 3 1 129 3 0.0 3 0 0 0 3 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 152 1 0.0 3 1 163 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 3 0.0 3 1 0 0 2 193 1 0.0 3 1 194 2 0.0 3 0 0 0 2 203 3 0.0 3 1 0 0 2 205 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 219 1 0.0 3 0 0 1 223 1 0.0 3 0 0 0 1 229 1 0.0 3 0 0 0 1 238 1 0.0 3 1 250 1 0.0 3 0 0 1 270 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 290 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.01 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 56,085 Reads with adapters: 1,194 (2.1%) Reads written (passing filters): 56,085 (100.0%) Total basepairs processed: 15,415,397 bp Total written (filtered): 15,411,772 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1194 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 61.8% C: 3.9% G: 33.0% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1152 876.3 0 1152 4 41 219.1 0 41 5 1 54.8 0 1