This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 138.02 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 112,364 (3.5%) Reads that were too short: 15,944 (0.5%) Reads written (passing filters): 96,420 (3.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 23,685,665 bp (2.5%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 112364 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 309 773.1 0 309 7 638 773.1 0 638 8 25281 773.1 0 25281 9 22727 773.1 0 22727 10 55 773.1 0 55 11 166 773.1 0 166 12 151 773.1 0 151 13 317 773.1 0 317 14 53 773.1 0 53 15 42 773.1 0 42 16 37 773.1 0 37 17 57 773.1 0 57 18 65 773.1 0 65 19 41 773.1 0 41 20 27 773.1 0 27 21 31 773.1 0 31 22 54 773.1 0 54 23 37 773.1 0 37 24 41 773.1 0 41 25 78 773.1 0 78 26 67 773.1 0 67 27 52 773.1 0 52 28 38 773.1 0 38 29 35 773.1 0 35 30 48 773.1 0 48 31 407 773.1 0 407 32 499 773.1 0 499 33 435 773.1 0 435 34 39 773.1 0 39 35 39 773.1 0 39 36 24 773.1 0 24 37 62 773.1 0 62 38 64 773.1 0 64 39 92 773.1 0 92 40 74 773.1 0 74 41 46 773.1 0 46 42 66 773.1 0 66 43 77 773.1 0 77 44 35 773.1 0 35 45 37 773.1 0 37 46 52 773.1 0 52 47 62 773.1 0 62 48 99 773.1 0 99 49 76 773.1 0 76 50 87 773.1 0 87 51 95 773.1 0 95 52 200 773.1 0 200 53 69 773.1 0 69 54 81 773.1 0 81 55 84 773.1 0 84 56 117 773.1 0 117 57 113 773.1 0 113 58 61 773.1 0 61 59 51 773.1 0 51 60 54 773.1 0 54 61 53 773.1 0 53 62 82 773.1 0 82 63 62 773.1 0 62 64 32 773.1 0 32 65 48 773.1 0 48 66 82 773.1 0 82 67 91 773.1 0 91 68 67 773.1 0 67 69 57 773.1 0 57 70 63 773.1 0 63 71 75 773.1 0 75 72 71 773.1 0 71 73 66 773.1 0 66 74 59 773.1 0 59 75 57 773.1 0 57 76 66 773.1 0 66 77 84 773.1 0 84 78 50 773.1 0 50 79 34 773.1 0 34 80 41 773.1 0 41 81 55 773.1 0 55 82 90 773.1 0 90 83 66 773.1 0 66 84 79 773.1 0 79 85 164 773.1 0 164 86 1079 773.1 0 1079 87 554 773.1 0 554 88 827 773.1 0 827 89 166 773.1 0 166 90 253 773.1 0 253 91 95 773.1 0 95 92 91 773.1 0 91 93 138 773.1 0 138 94 118 773.1 0 118 95 69 773.1 0 69 96 98 773.1 0 98 97 117 773.1 0 117 98 119 773.1 0 119 99 188 773.1 0 188 100 215 773.1 0 215 101 393 773.1 0 393 102 760 773.1 0 760 103 11726 773.1 0 11726 104 5726 773.1 0 5726 105 7785 773.1 0 7785 106 723 773.1 0 723 107 760 773.1 0 760 108 561 773.1 0 561 109 72 773.1 0 72 110 109 773.1 0 109 111 97 773.1 0 97 112 104 773.1 0 104 113 58 773.1 0 58 114 36 773.1 0 36 115 46 773.1 0 46 116 37 773.1 0 37 117 30 773.1 0 30 118 55 773.1 0 55 119 60 773.1 0 60 120 73 773.1 0 73 121 35 773.1 0 35 122 30 773.1 0 30 123 41 773.1 0 41 124 62 773.1 0 62 125 73 773.1 0 73 126 76 773.1 0 76 127 106 773.1 0 106 128 55 773.1 0 55 129 81 773.1 0 81 130 63 773.1 0 63 131 60 773.1 0 60 132 96 773.1 0 96 133 140 773.1 0 140 134 68 773.1 0 68 135 74 773.1 0 74 136 55 773.1 0 55 137 57 773.1 0 57 138 120 773.1 0 120 139 151 773.1 0 151 140 148 773.1 0 148 141 86 773.1 0 86 142 52 773.1 0 52 143 148 773.1 0 148 144 181 773.1 0 181 145 231 773.1 0 231 146 78 773.1 0 78 147 78 773.1 0 78 148 101 773.1 0 101 149 293 773.1 0 293 150 269 773.1 0 269 151 283 773.1 0 283 152 247 773.1 0 247 153 99 773.1 0 99 154 74 773.1 0 74 155 192 773.1 0 192 156 80 773.1 0 80 157 117 773.1 0 117 158 106 773.1 0 106 159 97 773.1 0 97 160 93 773.1 0 93 161 53 773.1 0 53 162 38 773.1 0 38 163 35 773.1 0 35 164 62 773.1 0 62 165 47 773.1 0 47 166 53 773.1 0 53 167 59 773.1 0 59 168 36 773.1 0 36 169 49 773.1 0 49 170 60 773.1 0 60 171 59 773.1 0 59 172 60 773.1 0 60 173 74 773.1 0 74 174 41 773.1 0 41 175 38 773.1 0 38 176 54 773.1 0 54 177 54 773.1 0 54 178 279 773.1 0 279 179 261 773.1 0 261 180 228 773.1 0 228 181 73 773.1 0 73 182 52 773.1 0 52 183 75 773.1 0 75 184 81 773.1 0 81 185 144 773.1 0 144 186 72 773.1 0 72 187 88 773.1 0 88 188 66 773.1 0 66 189 103 773.1 0 103 190 81 773.1 0 81 191 100 773.1 0 100 192 42 773.1 0 42 193 47 773.1 0 47 194 44 773.1 0 44 195 67 773.1 0 67 196 55 773.1 0 55 197 66 773.1 0 66 198 47 773.1 0 47 199 74 773.1 0 74 200 78 773.1 0 78 201 53 773.1 0 53 202 69 773.1 0 69 203 58 773.1 0 58 204 71 773.1 0 71 205 171 773.1 0 171 206 233 773.1 0 233 207 210 773.1 0 210 208 139 773.1 0 139 209 68 773.1 0 68 210 92 773.1 0 92 211 276 773.1 0 276 212 121 773.1 0 121 213 162 773.1 0 162 214 152 773.1 0 152 215 148 773.1 0 148 216 107 773.1 0 107 217 73 773.1 0 73 218 70 773.1 0 70 219 143 773.1 0 143 220 190 773.1 0 190 221 178 773.1 0 178 222 144 773.1 0 144 223 98 773.1 0 98 224 118 773.1 0 118 225 181 773.1 0 181 226 114 773.1 0 114 227 78 773.1 0 78 228 76 773.1 0 76 229 97 773.1 0 97 230 67 773.1 0 67 231 105 773.1 0 105 232 69 773.1 0 69 233 169 773.1 0 169 234 172 773.1 0 172 235 174 773.1 0 174 236 106 773.1 0 106 237 69 773.1 0 69 238 71 773.1 0 71 239 98 773.1 0 98 240 91 773.1 0 91 241 135 773.1 0 135 242 154 773.1 0 154 243 114 773.1 0 114 244 82 773.1 0 82 245 70 773.1 0 70 246 63 773.1 0 63 247 64 773.1 0 64 248 84 773.1 0 84 249 54 773.1 0 54 250 58 773.1 0 58 251 92 773.1 0 92 252 67 773.1 0 67 253 86 773.1 0 86 254 88 773.1 0 88 255 77 773.1 0 77 256 108 773.1 0 108 257 141 773.1 0 141 258 134 773.1 0 134 259 318 773.1 0 318 260 387 773.1 0 387 261 1885 773.1 0 1885 262 1391 773.1 0 1391 263 1226 773.1 0 1226 264 111 773.1 0 111 265 60 773.1 0 60 266 88 773.1 0 88 267 92 773.1 0 92 268 70 773.1 0 70 269 51 773.1 0 51 270 65 773.1 0 65 271 55 773.1 0 55 272 79 773.1 0 79 273 90 773.1 0 90 274 171 773.1 0 171 275 133 773.1 0 133 276 130 773.1 0 130 277 65 773.1 0 65 278 124 773.1 0 124 279 221 773.1 0 221 280 320 773.1 0 320 281 297 773.1 0 297 282 144 773.1 0 144 283 115 773.1 0 115 284 165 773.1 0 165 285 78 773.1 0 78 286 62 773.1 0 62 287 78 773.1 0 78 288 141 773.1 0 141 289 79 773.1 0 79 290 52 773.1 0 52 291 42 773.1 0 42 292 65 773.1 0 65 293 93 773.1 0 93 294 103 773.1 0 103 295 104 773.1 0 104 296 74 773.1 0 74 297 76 773.1 0 76 298 73 773.1 0 73 299 87 773.1 0 87 300 181 773.1 0 181 301 204 773.1 0 204 Finished in 6.52 s (68 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 96,420 Reads with adapters: 24,175 (25.1%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 24,145 (25.0%) Total basepairs processed: 23,685,665 bp Total written (filtered): 6,488,801 bp (27.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 24175 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 8 0.0 3 0 0 3 3 2 19 109 0.0 3 0 76 11 16 6 20 23115 0.0 4 21821 1016 197 60 21 21 77 0.0 4 10 59 4 3 1 22 14 0.0 4 4 0 3 2 5 23 77 0.0 4 0 0 0 54 23 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 1 26 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 56 2 0.0 4 1 0 0 0 1 58 1 0.0 4 0 0 0 0 1 75 2 0.0 4 0 0 0 0 2 81 1 0.0 4 0 0 0 0 1 93 3 0.0 4 0 0 0 0 3 97 1 0.0 4 0 0 0 0 1 112 16 0.0 4 0 0 0 0 16 116 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 1 123 3 0.0 4 1 0 0 0 2 124 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 5 0.0 4 0 0 0 1 4 127 704 0.0 4 0 0 0 0 704 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 135 3 0.0 4 0 0 0 0 3 136 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 1 197 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 1 6 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 274 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.38 s (57 us/read; 1.05 M reads/minute). === Summary === Total reads processed: 24,145 Reads with adapters: 5,068 (21.0%) Reads written (passing filters): 24,145 (100.0%) Total basepairs processed: 6,488,801 bp Total written (filtered): 6,432,419 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5068 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.8% C: 5.7% G: 90.5% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 377.3 0 6 4 4 94.3 0 4 7 2 1.5 0 2 8 2 0.4 0 2 9 1405 0.1 0 91 1314 10 2822 0.0 1 164 2658 11 778 0.0 1 23 755 12 12 0.0 1 0 12 13 7 0.0 1 0 7 14 1 0.0 1 0 1 208 4 0.0 1 3 1 223 18 0.0 1 16 2 257 6 0.0 1 6 258 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.