This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCCTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 136.59 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 105,308 (3.3%) Reads that were too short: 9,744 (0.3%) Reads written (passing filters): 95,564 (3.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 23,519,688 bp (2.5%) === Adapter 1 === Sequence: TTCCTT; Type: regular 5'; Length: 6; Trimmed: 105308 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 237 773.1 0 237 7 484 773.1 0 484 8 23422 773.1 0 23422 9 26079 773.1 0 26079 10 45 773.1 0 45 11 33 773.1 0 33 12 24 773.1 0 24 13 34 773.1 0 34 14 23 773.1 0 23 15 25 773.1 0 25 16 22 773.1 0 22 17 20 773.1 0 20 18 27 773.1 0 27 19 18 773.1 0 18 20 24 773.1 0 24 21 29 773.1 0 29 22 28 773.1 0 28 23 25 773.1 0 25 24 24 773.1 0 24 25 22 773.1 0 22 26 46 773.1 0 46 27 40 773.1 0 40 28 24 773.1 0 24 29 32 773.1 0 32 30 33 773.1 0 33 31 20 773.1 0 20 32 115 773.1 0 115 33 35 773.1 0 35 34 24 773.1 0 24 35 30 773.1 0 30 36 18 773.1 0 18 37 31 773.1 0 31 38 25 773.1 0 25 39 41 773.1 0 41 40 60 773.1 0 60 41 31 773.1 0 31 42 39 773.1 0 39 43 30 773.1 0 30 44 37 773.1 0 37 45 23 773.1 0 23 46 26 773.1 0 26 47 35 773.1 0 35 48 46 773.1 0 46 49 44 773.1 0 44 50 41 773.1 0 41 51 69 773.1 0 69 52 201 773.1 0 201 53 43 773.1 0 43 54 45 773.1 0 45 55 45 773.1 0 45 56 66 773.1 0 66 57 94 773.1 0 94 58 48 773.1 0 48 59 44 773.1 0 44 60 45 773.1 0 45 61 67 773.1 0 67 62 62 773.1 0 62 63 67 773.1 0 67 64 26 773.1 0 26 65 81 773.1 0 81 66 322 773.1 0 322 67 64 773.1 0 64 68 45 773.1 0 45 69 33 773.1 0 33 70 39 773.1 0 39 71 25 773.1 0 25 72 39 773.1 0 39 73 31 773.1 0 31 74 41 773.1 0 41 75 54 773.1 0 54 76 89 773.1 0 89 77 147 773.1 0 147 78 51 773.1 0 51 79 24 773.1 0 24 80 19 773.1 0 19 81 47 773.1 0 47 82 99 773.1 0 99 83 51 773.1 0 51 84 57 773.1 0 57 85 82 773.1 0 82 86 550 773.1 0 550 87 315 773.1 0 315 88 445 773.1 0 445 89 70 773.1 0 70 90 123 773.1 0 123 91 109 773.1 0 109 92 71 773.1 0 71 93 126 773.1 0 126 94 60 773.1 0 60 95 58 773.1 0 58 96 59 773.1 0 59 97 81 773.1 0 81 98 122 773.1 0 122 99 203 773.1 0 203 100 209 773.1 0 209 101 385 773.1 0 385 102 769 773.1 0 769 103 13906 773.1 0 13906 104 7079 773.1 0 7079 105 9361 773.1 0 9361 106 580 773.1 0 580 107 526 773.1 0 526 108 228 773.1 0 228 109 41 773.1 0 41 110 47 773.1 0 47 111 42 773.1 0 42 112 44 773.1 0 44 113 38 773.1 0 38 114 37 773.1 0 37 115 23 773.1 0 23 116 20 773.1 0 20 117 26 773.1 0 26 118 26 773.1 0 26 119 67 773.1 0 67 120 70 773.1 0 70 121 38 773.1 0 38 122 23 773.1 0 23 123 29 773.1 0 29 124 30 773.1 0 30 125 46 773.1 0 46 126 43 773.1 0 43 127 63 773.1 0 63 128 34 773.1 0 34 129 61 773.1 0 61 130 43 773.1 0 43 131 43 773.1 0 43 132 73 773.1 0 73 133 127 773.1 0 127 134 107 773.1 0 107 135 83 773.1 0 83 136 42 773.1 0 42 137 21 773.1 0 21 138 86 773.1 0 86 139 103 773.1 0 103 140 48 773.1 0 48 141 56 773.1 0 56 142 24 773.1 0 24 143 82 773.1 0 82 144 167 773.1 0 167 145 226 773.1 0 226 146 52 773.1 0 52 147 33 773.1 0 33 148 76 773.1 0 76 149 221 773.1 0 221 150 201 773.1 0 201 151 225 773.1 0 225 152 136 773.1 0 136 153 71 773.1 0 71 154 33 773.1 0 33 155 151 773.1 0 151 156 72 773.1 0 72 157 73 773.1 0 73 158 61 773.1 0 61 159 62 773.1 0 62 160 49 773.1 0 49 161 62 773.1 0 62 162 42 773.1 0 42 163 28 773.1 0 28 164 32 773.1 0 32 165 36 773.1 0 36 166 33 773.1 0 33 167 24 773.1 0 24 168 20 773.1 0 20 169 23 773.1 0 23 170 36 773.1 0 36 171 41 773.1 0 41 172 59 773.1 0 59 173 132 773.1 0 132 174 39 773.1 0 39 175 22 773.1 0 22 176 43 773.1 0 43 177 49 773.1 0 49 178 331 773.1 0 331 179 260 773.1 0 260 180 181 773.1 0 181 181 30 773.1 0 30 182 28 773.1 0 28 183 39 773.1 0 39 184 38 773.1 0 38 185 56 773.1 0 56 186 37 773.1 0 37 187 37 773.1 0 37 188 45 773.1 0 45 189 88 773.1 0 88 190 101 773.1 0 101 191 130 773.1 0 130 192 41 773.1 0 41 193 20 773.1 0 20 194 31 773.1 0 31 195 46 773.1 0 46 196 36 773.1 0 36 197 30 773.1 0 30 198 26 773.1 0 26 199 49 773.1 0 49 200 52 773.1 0 52 201 48 773.1 0 48 202 41 773.1 0 41 203 29 773.1 0 29 204 38 773.1 0 38 205 200 773.1 0 200 206 227 773.1 0 227 207 151 773.1 0 151 208 70 773.1 0 70 209 46 773.1 0 46 210 38 773.1 0 38 211 226 773.1 0 226 212 72 773.1 0 72 213 64 773.1 0 64 214 64 773.1 0 64 215 56 773.1 0 56 216 44 773.1 0 44 217 29 773.1 0 29 218 45 773.1 0 45 219 79 773.1 0 79 220 110 773.1 0 110 221 79 773.1 0 79 222 79 773.1 0 79 223 67 773.1 0 67 224 73 773.1 0 73 225 98 773.1 0 98 226 88 773.1 0 88 227 42 773.1 0 42 228 41 773.1 0 41 229 65 773.1 0 65 230 49 773.1 0 49 231 58 773.1 0 58 232 39 773.1 0 39 233 61 773.1 0 61 234 100 773.1 0 100 235 93 773.1 0 93 236 58 773.1 0 58 237 56 773.1 0 56 238 46 773.1 0 46 239 41 773.1 0 41 240 39 773.1 0 39 241 81 773.1 0 81 242 93 773.1 0 93 243 78 773.1 0 78 244 53 773.1 0 53 245 44 773.1 0 44 246 36 773.1 0 36 247 38 773.1 0 38 248 45 773.1 0 45 249 29 773.1 0 29 250 30 773.1 0 30 251 49 773.1 0 49 252 52 773.1 0 52 253 63 773.1 0 63 254 38 773.1 0 38 255 47 773.1 0 47 256 59 773.1 0 59 257 69 773.1 0 69 258 88 773.1 0 88 259 253 773.1 0 253 260 272 773.1 0 272 261 1271 773.1 0 1271 262 738 773.1 0 738 263 775 773.1 0 775 264 78 773.1 0 78 265 53 773.1 0 53 266 40 773.1 0 40 267 49 773.1 0 49 268 42 773.1 0 42 269 34 773.1 0 34 270 36 773.1 0 36 271 33 773.1 0 33 272 40 773.1 0 40 273 37 773.1 0 37 274 113 773.1 0 113 275 93 773.1 0 93 276 82 773.1 0 82 277 45 773.1 0 45 278 62 773.1 0 62 279 152 773.1 0 152 280 171 773.1 0 171 281 155 773.1 0 155 282 72 773.1 0 72 283 42 773.1 0 42 284 86 773.1 0 86 285 52 773.1 0 52 286 26 773.1 0 26 287 60 773.1 0 60 288 102 773.1 0 102 289 66 773.1 0 66 290 43 773.1 0 43 291 26 773.1 0 26 292 39 773.1 0 39 293 65 773.1 0 65 294 60 773.1 0 60 295 77 773.1 0 77 296 51 773.1 0 51 297 61 773.1 0 61 298 47 773.1 0 47 299 63 773.1 0 63 300 80 773.1 0 80 301 109 773.1 0 109 Finished in 5.99 s (63 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 95,564 Reads with adapters: 23,359 (24.4%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 23,325 (24.4%) Total basepairs processed: 23,519,688 bp Total written (filtered): 6,435,467 bp (27.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 23359 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 111 0.0 3 0 107 4 17 23110 0.0 3 22620 465 20 5 18 53 0.0 3 1 46 0 6 19 12 0.0 3 1 0 10 1 20 17 0.0 3 0 0 0 17 27 2 0.0 3 0 0 1 1 28 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 1 0.0 3 1 66 1 0.0 3 0 0 0 1 67 3 0.0 3 0 0 0 3 70 1 0.0 3 0 0 0 1 75 4 0.0 3 1 0 0 3 82 1 0.0 3 0 0 0 1 93 3 0.0 3 0 0 0 3 99 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 121 3 0.0 3 0 0 0 3 125 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 168 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 187 3 0.0 3 0 0 0 3 194 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 209 1 0.0 3 0 0 0 1 239 2 0.0 3 0 0 0 2 269 1 0.0 3 0 0 1 280 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.26 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 23,325 Reads with adapters: 202 (0.9%) Reads written (passing filters): 23,325 (100.0%) Total basepairs processed: 6,435,467 bp Total written (filtered): 6,434,847 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 202 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 87.6% C: 5.0% G: 4.5% T: 3.0% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 188 364.5 0 188 4 14 91.1 0 14 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.