This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 143.64 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 366,136 (11.6%) Reads that were too short: 164,385 (5.2%) Reads written (passing filters): 201,751 (6.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 52,280,727 bp (5.5%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 366136 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1226 773.1 0 1226 7 64999 773.1 0 64999 8 52346 773.1 0 52346 9 125 773.1 0 125 10 47 773.1 0 47 11 53 773.1 0 53 12 27 773.1 0 27 13 44 773.1 0 44 14 42 773.1 0 42 15 29 773.1 0 29 16 57 773.1 0 57 17 45 773.1 0 45 18 65 773.1 0 65 19 75 773.1 0 75 20 9802 773.1 0 9802 21 9243 773.1 0 9243 22 164 773.1 0 164 23 127 773.1 0 127 24 50 773.1 0 50 25 43 773.1 0 43 26 515 773.1 0 515 27 35 773.1 0 35 28 43 773.1 0 43 29 37 773.1 0 37 30 44 773.1 0 44 31 48 773.1 0 48 32 44 773.1 0 44 33 7223 773.1 0 7223 34 685 773.1 0 685 35 37 773.1 0 37 36 47 773.1 0 47 37 49 773.1 0 49 38 70 773.1 0 70 39 91 773.1 0 91 40 52 773.1 0 52 41 50 773.1 0 50 42 49 773.1 0 49 43 62 773.1 0 62 44 64 773.1 0 64 45 72 773.1 0 72 46 50 773.1 0 50 47 55 773.1 0 55 48 65 773.1 0 65 49 57 773.1 0 57 50 96 773.1 0 96 51 47 773.1 0 47 52 67 773.1 0 67 53 112 773.1 0 112 54 51 773.1 0 51 55 73 773.1 0 73 56 66 773.1 0 66 57 81 773.1 0 81 58 97 773.1 0 97 59 78 773.1 0 78 60 88 773.1 0 88 61 96 773.1 0 96 62 96 773.1 0 96 63 124 773.1 0 124 64 83 773.1 0 83 65 105 773.1 0 105 66 139 773.1 0 139 67 116 773.1 0 116 68 132 773.1 0 132 69 165 773.1 0 165 70 146 773.1 0 146 71 178 773.1 0 178 72 109 773.1 0 109 73 164 773.1 0 164 74 186 773.1 0 186 75 435 773.1 0 435 76 1621 773.1 0 1621 77 1885 773.1 0 1885 78 3282 773.1 0 3282 79 213 773.1 0 213 80 353 773.1 0 353 81 424 773.1 0 424 82 540 773.1 0 540 83 451 773.1 0 451 84 342 773.1 0 342 85 370 773.1 0 370 86 133 773.1 0 133 87 157 773.1 0 157 88 557 773.1 0 557 89 164 773.1 0 164 90 143 773.1 0 143 91 191 773.1 0 191 92 191 773.1 0 191 93 168 773.1 0 168 94 154 773.1 0 154 95 176 773.1 0 176 96 153 773.1 0 153 97 168 773.1 0 168 98 160 773.1 0 160 99 186 773.1 0 186 100 172 773.1 0 172 101 187 773.1 0 187 102 153 773.1 0 153 103 192 773.1 0 192 104 216 773.1 0 216 105 173 773.1 0 173 106 211 773.1 0 211 107 202 773.1 0 202 108 216 773.1 0 216 109 206 773.1 0 206 110 196 773.1 0 196 111 264 773.1 0 264 112 272 773.1 0 272 113 226 773.1 0 226 114 210 773.1 0 210 115 260 773.1 0 260 116 251 773.1 0 251 117 255 773.1 0 255 118 231 773.1 0 231 119 228 773.1 0 228 120 248 773.1 0 248 121 245 773.1 0 245 122 203 773.1 0 203 123 434 773.1 0 434 124 277 773.1 0 277 125 274 773.1 0 274 126 265 773.1 0 265 127 256 773.1 0 256 128 277 773.1 0 277 129 359 773.1 0 359 130 2198 773.1 0 2198 131 1623 773.1 0 1623 132 2043 773.1 0 2043 133 250 773.1 0 250 134 293 773.1 0 293 135 277 773.1 0 277 136 1404 773.1 0 1404 137 1743 773.1 0 1743 138 2574 773.1 0 2574 139 752 773.1 0 752 140 560 773.1 0 560 141 348 773.1 0 348 142 303 773.1 0 303 143 613 773.1 0 613 144 227 773.1 0 227 145 214 773.1 0 214 146 515 773.1 0 515 147 408 773.1 0 408 148 641 773.1 0 641 149 285 773.1 0 285 150 247 773.1 0 247 151 245 773.1 0 245 152 263 773.1 0 263 153 233 773.1 0 233 154 251 773.1 0 251 155 201 773.1 0 201 156 250 773.1 0 250 157 243 773.1 0 243 158 213 773.1 0 213 159 187 773.1 0 187 160 265 773.1 0 265 161 276 773.1 0 276 162 373 773.1 0 373 163 235 773.1 0 235 164 256 773.1 0 256 165 313 773.1 0 313 166 244 773.1 0 244 167 254 773.1 0 254 168 354 773.1 0 354 169 184 773.1 0 184 170 225 773.1 0 225 171 278 773.1 0 278 172 251 773.1 0 251 173 217 773.1 0 217 174 200 773.1 0 200 175 217 773.1 0 217 176 257 773.1 0 257 177 225 773.1 0 225 178 210 773.1 0 210 179 247 773.1 0 247 180 193 773.1 0 193 181 367 773.1 0 367 182 217 773.1 0 217 183 225 773.1 0 225 184 241 773.1 0 241 185 239 773.1 0 239 186 359 773.1 0 359 187 327 773.1 0 327 188 286 773.1 0 286 189 363 773.1 0 363 190 309 773.1 0 309 191 238 773.1 0 238 192 319 773.1 0 319 193 275 773.1 0 275 194 291 773.1 0 291 195 302 773.1 0 302 196 370 773.1 0 370 197 482 773.1 0 482 198 323 773.1 0 323 199 251 773.1 0 251 200 217 773.1 0 217 201 248 773.1 0 248 202 275 773.1 0 275 203 215 773.1 0 215 204 225 773.1 0 225 205 238 773.1 0 238 206 257 773.1 0 257 207 226 773.1 0 226 208 279 773.1 0 279 209 290 773.1 0 290 210 295 773.1 0 295 211 323 773.1 0 323 212 260 773.1 0 260 213 335 773.1 0 335 214 626 773.1 0 626 215 313 773.1 0 313 216 263 773.1 0 263 217 269 773.1 0 269 218 281 773.1 0 281 219 288 773.1 0 288 220 312 773.1 0 312 221 792 773.1 0 792 222 379 773.1 0 379 223 318 773.1 0 318 224 404 773.1 0 404 225 492 773.1 0 492 226 714 773.1 0 714 227 820 773.1 0 820 228 891 773.1 0 891 229 640 773.1 0 640 230 801 773.1 0 801 231 787 773.1 0 787 232 640 773.1 0 640 233 799 773.1 0 799 234 606 773.1 0 606 235 395 773.1 0 395 236 438 773.1 0 438 237 613 773.1 0 613 238 1237 773.1 0 1237 239 561 773.1 0 561 240 709 773.1 0 709 241 1250 773.1 0 1250 242 727 773.1 0 727 243 675 773.1 0 675 244 931 773.1 0 931 245 653 773.1 0 653 246 1074 773.1 0 1074 247 825 773.1 0 825 248 1361 773.1 0 1361 249 1314 773.1 0 1314 250 2078 773.1 0 2078 251 40622 773.1 0 40622 252 28422 773.1 0 28422 253 47209 773.1 0 47209 254 723 773.1 0 723 255 317 773.1 0 317 256 487 773.1 0 487 257 485 773.1 0 485 258 687 773.1 0 687 259 357 773.1 0 357 260 378 773.1 0 378 261 309 773.1 0 309 262 335 773.1 0 335 263 278 773.1 0 278 264 307 773.1 0 307 265 379 773.1 0 379 266 399 773.1 0 399 267 312 773.1 0 312 268 297 773.1 0 297 269 341 773.1 0 341 270 381 773.1 0 381 271 361 773.1 0 361 272 368 773.1 0 368 273 284 773.1 0 284 274 251 773.1 0 251 275 255 773.1 0 255 276 318 773.1 0 318 277 304 773.1 0 304 278 501 773.1 0 501 279 273 773.1 0 273 280 278 773.1 0 278 281 374 773.1 0 374 282 234 773.1 0 234 283 398 773.1 0 398 284 410 773.1 0 410 285 529 773.1 0 529 286 401 773.1 0 401 287 372 773.1 0 372 288 332 773.1 0 332 289 289 773.1 0 289 290 395 773.1 0 395 291 487 773.1 0 487 292 403 773.1 0 403 293 313 773.1 0 313 294 344 773.1 0 344 295 324 773.1 0 324 296 337 773.1 0 337 297 615 773.1 0 615 298 795 773.1 0 795 299 509 773.1 0 509 300 422 773.1 0 422 301 390 773.1 0 390 Finished in 14.35 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 201,751 Reads with adapters: 54,511 (27.0%) Reads that were too short: 155 (0.1%) Reads written (passing filters): 54,356 (26.9%) Total basepairs processed: 52,280,727 bp Total written (filtered): 14,805,418 bp (28.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 54511 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 3 5 18 44 0.0 3 0 0 6 16 22 19 406 0.0 3 0 242 60 69 35 20 53118 0.0 4 49159 2978 679 227 75 21 165 0.0 4 32 114 8 6 5 22 14 0.0 4 1 0 10 0 3 23 263 0.0 4 3 0 2 174 84 24 4 0.0 4 0 0 0 2 2 27 2 0.0 4 1 0 0 0 1 31 1 0.0 4 1 35 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 54 27 0.0 4 0 0 0 0 27 55 1 0.0 4 0 0 0 0 1 57 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 65 2 0.0 4 0 0 0 0 2 66 2 0.0 4 0 0 0 0 2 68 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 2 0.0 4 0 0 0 0 2 72 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 80 2 0.0 4 0 0 0 0 2 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 3 0.0 4 0 0 0 0 3 85 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 2 0.0 4 1 0 0 1 96 2 0.0 4 0 0 0 1 1 99 20 0.0 4 0 0 0 0 20 100 7 0.0 4 0 0 0 2 5 101 2 0.0 4 0 0 1 0 1 102 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 111 2 0.0 4 0 0 0 0 2 113 3 0.0 4 0 0 0 0 3 114 1 0.0 4 0 0 0 0 1 115 2 0.0 4 0 0 0 0 2 117 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 5 0.0 4 0 0 0 0 5 122 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 243 0.0 4 0 1 0 0 242 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 148 1 0.0 4 0 0 0 0 1 150 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 1 153 1 0.0 4 0 0 1 154 2 0.0 4 1 1 156 3 0.0 4 1 1 1 157 1 0.0 4 0 0 0 0 1 159 9 0.0 4 0 0 0 0 9 160 5 0.0 4 0 0 0 0 5 161 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 1 175 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 181 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 189 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 1 207 1 0.0 4 0 0 0 0 1 211 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 1 213 2 0.0 4 0 0 0 0 2 214 1 0.0 4 0 0 0 0 1 217 3 0.0 4 0 0 0 0 3 218 1 0.0 4 0 0 0 0 1 221 1 0.0 4 0 0 0 0 1 223 2 0.0 4 0 0 0 0 2 226 1 0.0 4 0 0 0 0 1 227 2 0.0 4 0 0 0 0 2 228 15 0.0 4 0 0 0 1 14 229 8 0.0 4 0 0 0 0 8 230 1 0.0 4 0 0 0 0 1 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 2 0.0 4 0 0 0 0 2 237 5 0.0 4 0 0 0 0 5 238 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 0 0 0 1 243 1 0.0 4 0 0 0 0 1 246 1 0.0 4 0 0 0 0 1 247 2 0.0 4 0 0 0 0 2 256 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 1 1 262 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 274 2 0.0 4 0 0 0 0 2 276 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.98 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 54,356 Reads with adapters: 7,300 (13.4%) Reads written (passing filters): 54,356 (100.0%) Total basepairs processed: 14,805,418 bp Total written (filtered): 14,723,530 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7300 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 6.4% G: 89.2% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 98 849.3 0 98 4 19 212.3 0 19 5 1 53.1 0 1 7 1 3.3 0 1 8 1 0.8 0 1 9 316 0.2 0 15 301 10 1136 0.1 1 67 1069 11 5594 0.0 1 122 5472 12 95 0.0 1 1 94 13 6 0.0 1 1 5 14 4 0.0 1 0 4 15 5 0.0 1 0 5 52 1 0.0 1 0 1 123 3 0.0 1 3 151 3 0.0 1 3 190 4 0.0 1 2 2 209 9 0.0 1 8 1 224 4 0.0 1 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.