This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGTAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 140.34 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 315,891 (10.0%) Reads that were too short: 159,472 (5.0%) Reads written (passing filters): 156,419 (4.9%) Total basepairs processed: 953,096,634 bp Total written (filtered): 42,731,057 bp (4.5%) === Adapter 1 === Sequence: GTGTAG; Type: regular 5'; Length: 6; Trimmed: 315891 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1174 773.1 0 1174 7 56704 773.1 0 56704 8 68097 773.1 0 68097 9 139 773.1 0 139 10 63 773.1 0 63 11 63 773.1 0 63 12 45 773.1 0 45 13 44 773.1 0 44 14 44 773.1 0 44 15 33 773.1 0 33 16 40 773.1 0 40 17 49 773.1 0 49 18 66 773.1 0 66 19 50 773.1 0 50 20 49 773.1 0 49 21 71 773.1 0 71 22 70 773.1 0 70 23 82 773.1 0 82 24 50 773.1 0 50 25 44 773.1 0 44 26 42 773.1 0 42 27 37 773.1 0 37 28 64 773.1 0 64 29 44 773.1 0 44 30 55 773.1 0 55 31 58 773.1 0 58 32 49 773.1 0 49 33 43 773.1 0 43 34 45 773.1 0 45 35 41 773.1 0 41 36 65 773.1 0 65 37 41 773.1 0 41 38 88 773.1 0 88 39 78 773.1 0 78 40 77 773.1 0 77 41 44 773.1 0 44 42 35 773.1 0 35 43 67 773.1 0 67 44 60 773.1 0 60 45 63 773.1 0 63 46 59 773.1 0 59 47 69 773.1 0 69 48 110 773.1 0 110 49 80 773.1 0 80 50 199 773.1 0 199 51 43 773.1 0 43 52 56 773.1 0 56 53 57 773.1 0 57 54 67 773.1 0 67 55 84 773.1 0 84 56 51 773.1 0 51 57 61 773.1 0 61 58 101 773.1 0 101 59 62 773.1 0 62 60 81 773.1 0 81 61 78 773.1 0 78 62 45 773.1 0 45 63 94 773.1 0 94 64 59 773.1 0 59 65 73 773.1 0 73 66 65 773.1 0 65 67 83 773.1 0 83 68 63 773.1 0 63 69 116 773.1 0 116 70 87 773.1 0 87 71 84 773.1 0 84 72 69 773.1 0 69 73 76 773.1 0 76 74 74 773.1 0 74 75 315 773.1 0 315 76 1380 773.1 0 1380 77 1427 773.1 0 1427 78 2170 773.1 0 2170 79 140 773.1 0 140 80 191 773.1 0 191 81 198 773.1 0 198 82 182 773.1 0 182 83 387 773.1 0 387 84 314 773.1 0 314 85 327 773.1 0 327 86 42 773.1 0 42 87 59 773.1 0 59 88 58 773.1 0 58 89 60 773.1 0 60 90 56 773.1 0 56 91 93 773.1 0 93 92 75 773.1 0 75 93 70 773.1 0 70 94 70 773.1 0 70 95 73 773.1 0 73 96 63 773.1 0 63 97 58 773.1 0 58 98 60 773.1 0 60 99 63 773.1 0 63 100 74 773.1 0 74 101 61 773.1 0 61 102 83 773.1 0 83 103 73 773.1 0 73 104 84 773.1 0 84 105 87 773.1 0 87 106 83 773.1 0 83 107 112 773.1 0 112 108 80 773.1 0 80 109 74 773.1 0 74 110 67 773.1 0 67 111 107 773.1 0 107 112 143 773.1 0 143 113 75 773.1 0 75 114 64 773.1 0 64 115 87 773.1 0 87 116 76 773.1 0 76 117 87 773.1 0 87 118 69 773.1 0 69 119 81 773.1 0 81 120 106 773.1 0 106 121 110 773.1 0 110 122 89 773.1 0 89 123 127 773.1 0 127 124 92 773.1 0 92 125 71 773.1 0 71 126 96 773.1 0 96 127 85 773.1 0 85 128 80 773.1 0 80 129 109 773.1 0 109 130 1525 773.1 0 1525 131 1069 773.1 0 1069 132 1355 773.1 0 1355 133 124 773.1 0 124 134 119 773.1 0 119 135 120 773.1 0 120 136 1230 773.1 0 1230 137 1615 773.1 0 1615 138 2154 773.1 0 2154 139 259 773.1 0 259 140 71 773.1 0 71 141 88 773.1 0 88 142 109 773.1 0 109 143 83 773.1 0 83 144 60 773.1 0 60 145 70 773.1 0 70 146 487 773.1 0 487 147 278 773.1 0 278 148 524 773.1 0 524 149 126 773.1 0 126 150 44 773.1 0 44 151 52 773.1 0 52 152 40 773.1 0 40 153 66 773.1 0 66 154 117 773.1 0 117 155 48 773.1 0 48 156 48 773.1 0 48 157 41 773.1 0 41 158 64 773.1 0 64 159 49 773.1 0 49 160 80 773.1 0 80 161 154 773.1 0 154 162 164 773.1 0 164 163 86 773.1 0 86 164 149 773.1 0 149 165 178 773.1 0 178 166 87 773.1 0 87 167 95 773.1 0 95 168 199 773.1 0 199 169 58 773.1 0 58 170 64 773.1 0 64 171 149 773.1 0 149 172 58 773.1 0 58 173 59 773.1 0 59 174 44 773.1 0 44 175 67 773.1 0 67 176 63 773.1 0 63 177 61 773.1 0 61 178 54 773.1 0 54 179 33 773.1 0 33 180 36 773.1 0 36 181 44 773.1 0 44 182 72 773.1 0 72 183 74 773.1 0 74 184 76 773.1 0 76 185 76 773.1 0 76 186 155 773.1 0 155 187 94 773.1 0 94 188 105 773.1 0 105 189 190 773.1 0 190 190 130 773.1 0 130 191 55 773.1 0 55 192 47 773.1 0 47 193 45 773.1 0 45 194 44 773.1 0 44 195 47 773.1 0 47 196 61 773.1 0 61 197 43 773.1 0 43 198 55 773.1 0 55 199 36 773.1 0 36 200 36 773.1 0 36 201 79 773.1 0 79 202 58 773.1 0 58 203 37 773.1 0 37 204 32 773.1 0 32 205 37 773.1 0 37 206 48 773.1 0 48 207 63 773.1 0 63 208 49 773.1 0 49 209 69 773.1 0 69 210 59 773.1 0 59 211 51 773.1 0 51 212 41 773.1 0 41 213 66 773.1 0 66 214 55 773.1 0 55 215 52 773.1 0 52 216 71 773.1 0 71 217 71 773.1 0 71 218 58 773.1 0 58 219 73 773.1 0 73 220 61 773.1 0 61 221 82 773.1 0 82 222 93 773.1 0 93 223 82 773.1 0 82 224 147 773.1 0 147 225 296 773.1 0 296 226 453 773.1 0 453 227 489 773.1 0 489 228 506 773.1 0 506 229 382 773.1 0 382 230 539 773.1 0 539 231 572 773.1 0 572 232 347 773.1 0 347 233 554 773.1 0 554 234 183 773.1 0 183 235 202 773.1 0 202 236 305 773.1 0 305 237 504 773.1 0 504 238 1175 773.1 0 1175 239 300 773.1 0 300 240 482 773.1 0 482 241 824 773.1 0 824 242 329 773.1 0 329 243 286 773.1 0 286 244 461 773.1 0 461 245 394 773.1 0 394 246 852 773.1 0 852 247 677 773.1 0 677 248 1375 773.1 0 1375 249 1159 773.1 0 1159 250 1838 773.1 0 1838 251 46970 773.1 0 46970 252 33644 773.1 0 33644 253 54894 773.1 0 54894 254 431 773.1 0 431 255 94 773.1 0 94 256 116 773.1 0 116 257 112 773.1 0 112 258 159 773.1 0 159 259 66 773.1 0 66 260 96 773.1 0 96 261 79 773.1 0 79 262 138 773.1 0 138 263 105 773.1 0 105 264 105 773.1 0 105 265 111 773.1 0 111 266 118 773.1 0 118 267 115 773.1 0 115 268 93 773.1 0 93 269 116 773.1 0 116 270 141 773.1 0 141 271 129 773.1 0 129 272 100 773.1 0 100 273 100 773.1 0 100 274 83 773.1 0 83 275 82 773.1 0 82 276 83 773.1 0 83 277 87 773.1 0 87 278 76 773.1 0 76 279 82 773.1 0 82 280 87 773.1 0 87 281 78 773.1 0 78 282 69 773.1 0 69 283 232 773.1 0 232 284 233 773.1 0 233 285 315 773.1 0 315 286 242 773.1 0 242 287 134 773.1 0 134 288 130 773.1 0 130 289 118 773.1 0 118 290 149 773.1 0 149 291 205 773.1 0 205 292 180 773.1 0 180 293 149 773.1 0 149 294 144 773.1 0 144 295 148 773.1 0 148 296 126 773.1 0 126 297 337 773.1 0 337 298 361 773.1 0 361 299 224 773.1 0 224 300 189 773.1 0 189 301 158 773.1 0 158 Finished in 10.48 s (67 us/read; 0.90 M reads/minute). === Summary === Total reads processed: 156,419 Reads with adapters: 55,275 (35.3%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 55,241 (35.3%) Total basepairs processed: 42,731,057 bp Total written (filtered): 15,289,147 bp (35.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 55275 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 7 0.0 3 0 0 7 16 193 0.0 3 0 187 5 1 17 54748 0.0 3 53810 902 30 6 18 134 0.0 3 14 115 4 1 19 17 0.0 3 2 0 14 1 20 62 0.0 3 1 0 0 61 34 1 0.0 3 1 35 10 0.0 3 0 0 0 10 36 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 61 35 0.0 3 0 0 0 35 64 4 0.0 3 0 0 0 4 65 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 80 1 0.0 3 1 86 1 0.0 3 0 0 0 1 88 1 0.0 3 1 90 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 97 2 0.0 3 0 0 0 2 100 4 0.0 3 0 0 0 4 101 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 1 1 110 2 0.0 3 0 0 0 2 111 4 0.0 3 0 0 0 4 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 2 0.0 3 0 0 0 2 121 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 2 0.0 3 1 0 0 1 155 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 217 7 0.0 3 0 0 0 7 264 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.00 s (54 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 55,241 Reads with adapters: 74 (0.1%) Reads written (passing filters): 55,241 (100.0%) Total basepairs processed: 15,289,147 bp Total written (filtered): 15,288,897 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 74 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.3% C: 12.2% G: 51.4% T: 16.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 52 863.1 0 52 4 16 215.8 0 16 5 6 53.9 0 6