This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 140.54 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 285,837 (9.0%) Reads that were too short: 243,130 (7.7%) Reads written (passing filters): 42,707 (1.3%) Total basepairs processed: 953,096,634 bp Total written (filtered): 9,404,803 bp (1.0%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 285837 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3553 773.1 0 3553 7 7753 773.1 0 7753 8 717 773.1 0 717 9 8969 773.1 0 8969 10 37 773.1 0 37 11 25 773.1 0 25 12 17 773.1 0 17 13 31 773.1 0 31 14 15 773.1 0 15 15 17 773.1 0 17 16 24 773.1 0 24 17 19 773.1 0 19 18 40 773.1 0 40 19 49 773.1 0 49 20 57 773.1 0 57 21 35 773.1 0 35 22 36 773.1 0 36 23 22 773.1 0 22 24 9 773.1 0 9 25 17 773.1 0 17 26 15 773.1 0 15 27 21 773.1 0 21 28 11 773.1 0 11 29 14 773.1 0 14 30 14 773.1 0 14 31 18 773.1 0 18 32 16 773.1 0 16 33 17 773.1 0 17 34 15 773.1 0 15 35 27 773.1 0 27 36 20 773.1 0 20 37 17 773.1 0 17 38 15 773.1 0 15 39 27 773.1 0 27 40 24 773.1 0 24 41 30 773.1 0 30 42 23 773.1 0 23 43 29 773.1 0 29 44 18 773.1 0 18 45 34 773.1 0 34 46 58 773.1 0 58 47 212 773.1 0 212 48 57 773.1 0 57 49 68 773.1 0 68 50 23 773.1 0 23 51 25 773.1 0 25 52 29 773.1 0 29 53 49 773.1 0 49 54 52 773.1 0 52 55 93 773.1 0 93 56 40 773.1 0 40 57 25 773.1 0 25 58 21 773.1 0 21 59 30 773.1 0 30 60 32 773.1 0 32 61 30 773.1 0 30 62 39 773.1 0 39 63 42 773.1 0 42 64 38 773.1 0 38 65 21 773.1 0 21 66 21 773.1 0 21 67 23 773.1 0 23 68 12 773.1 0 12 69 50 773.1 0 50 70 47 773.1 0 47 71 84 773.1 0 84 72 132 773.1 0 132 73 117 773.1 0 117 74 166 773.1 0 166 75 14 773.1 0 14 76 27 773.1 0 27 77 12 773.1 0 12 78 14 773.1 0 14 79 13 773.1 0 13 80 13 773.1 0 13 81 22 773.1 0 22 82 22 773.1 0 22 83 60 773.1 0 60 84 48 773.1 0 48 85 43 773.1 0 43 86 44 773.1 0 44 87 40 773.1 0 40 88 19 773.1 0 19 89 29 773.1 0 29 90 28 773.1 0 28 91 30 773.1 0 30 92 12 773.1 0 12 93 20 773.1 0 20 94 18 773.1 0 18 95 22 773.1 0 22 96 17 773.1 0 17 97 19 773.1 0 19 98 14 773.1 0 14 99 13 773.1 0 13 100 19 773.1 0 19 101 18 773.1 0 18 102 16 773.1 0 16 103 26 773.1 0 26 104 22 773.1 0 22 105 32 773.1 0 32 106 16 773.1 0 16 107 28 773.1 0 28 108 31 773.1 0 31 109 19 773.1 0 19 110 20 773.1 0 20 111 19 773.1 0 19 112 30 773.1 0 30 113 50 773.1 0 50 114 63 773.1 0 63 115 41 773.1 0 41 116 78 773.1 0 78 117 57 773.1 0 57 118 72 773.1 0 72 119 28 773.1 0 28 120 42 773.1 0 42 121 27 773.1 0 27 122 64 773.1 0 64 123 328 773.1 0 328 124 275 773.1 0 275 125 706 773.1 0 706 126 132 773.1 0 132 127 46 773.1 0 46 128 46 773.1 0 46 129 44 773.1 0 44 130 46 773.1 0 46 131 57 773.1 0 57 132 33 773.1 0 33 133 33 773.1 0 33 134 46 773.1 0 46 135 48 773.1 0 48 136 49 773.1 0 49 137 40 773.1 0 40 138 33 773.1 0 33 139 58 773.1 0 58 140 39 773.1 0 39 141 71 773.1 0 71 142 30 773.1 0 30 143 37 773.1 0 37 144 44 773.1 0 44 145 42 773.1 0 42 146 38 773.1 0 38 147 71 773.1 0 71 148 24 773.1 0 24 149 71 773.1 0 71 150 245 773.1 0 245 151 183 773.1 0 183 152 241 773.1 0 241 153 94 773.1 0 94 154 64 773.1 0 64 155 57 773.1 0 57 156 60 773.1 0 60 157 45 773.1 0 45 158 56 773.1 0 56 159 114 773.1 0 114 160 419 773.1 0 419 161 276 773.1 0 276 162 542 773.1 0 542 163 130 773.1 0 130 164 153 773.1 0 153 165 1258 773.1 0 1258 166 922 773.1 0 922 167 1550 773.1 0 1550 168 293 773.1 0 293 169 131 773.1 0 131 170 106 773.1 0 106 171 107 773.1 0 107 172 124 773.1 0 124 173 97 773.1 0 97 174 70 773.1 0 70 175 98 773.1 0 98 176 135 773.1 0 135 177 157 773.1 0 157 178 114 773.1 0 114 179 214 773.1 0 214 180 187 773.1 0 187 181 159 773.1 0 159 182 118 773.1 0 118 183 219 773.1 0 219 184 102 773.1 0 102 185 759 773.1 0 759 186 928 773.1 0 928 187 1472 773.1 0 1472 188 776 773.1 0 776 189 728 773.1 0 728 190 94 773.1 0 94 191 105 773.1 0 105 192 97 773.1 0 97 193 84 773.1 0 84 194 103 773.1 0 103 195 150 773.1 0 150 196 191 773.1 0 191 197 228 773.1 0 228 198 211 773.1 0 211 199 171 773.1 0 171 200 263 773.1 0 263 201 106 773.1 0 106 202 66 773.1 0 66 203 65 773.1 0 65 204 84 773.1 0 84 205 77 773.1 0 77 206 63 773.1 0 63 207 74 773.1 0 74 208 60 773.1 0 60 209 88 773.1 0 88 210 263 773.1 0 263 211 73 773.1 0 73 212 108 773.1 0 108 213 100 773.1 0 100 214 175 773.1 0 175 215 116 773.1 0 116 216 150 773.1 0 150 217 268 773.1 0 268 218 498 773.1 0 498 219 665 773.1 0 665 220 1113 773.1 0 1113 221 5828 773.1 0 5828 222 7569 773.1 0 7569 223 7207 773.1 0 7207 224 3679 773.1 0 3679 225 955 773.1 0 955 226 663 773.1 0 663 227 1061 773.1 0 1061 228 238 773.1 0 238 229 251 773.1 0 251 230 235 773.1 0 235 231 354 773.1 0 354 232 520 773.1 0 520 233 713 773.1 0 713 234 883 773.1 0 883 235 1912 773.1 0 1912 236 4438 773.1 0 4438 237 58741 773.1 0 58741 238 38194 773.1 0 38194 239 71166 773.1 0 71166 240 3814 773.1 0 3814 241 1019 773.1 0 1019 242 318 773.1 0 318 243 148 773.1 0 148 244 164 773.1 0 164 245 2513 773.1 0 2513 246 3501 773.1 0 3501 247 4340 773.1 0 4340 248 2316 773.1 0 2316 249 1107 773.1 0 1107 250 847 773.1 0 847 251 263 773.1 0 263 252 108 773.1 0 108 253 39 773.1 0 39 254 41 773.1 0 41 255 47 773.1 0 47 256 98 773.1 0 98 257 51 773.1 0 51 258 51 773.1 0 51 259 45 773.1 0 45 260 112 773.1 0 112 261 115 773.1 0 115 262 166 773.1 0 166 263 71 773.1 0 71 264 87 773.1 0 87 265 64 773.1 0 64 266 111 773.1 0 111 267 274 773.1 0 274 268 738 773.1 0 738 269 712 773.1 0 712 270 930 773.1 0 930 271 383 773.1 0 383 272 192 773.1 0 192 273 124 773.1 0 124 274 211 773.1 0 211 275 118 773.1 0 118 276 120 773.1 0 120 277 109 773.1 0 109 278 123 773.1 0 123 279 115 773.1 0 115 280 204 773.1 0 204 281 635 773.1 0 635 282 423 773.1 0 423 283 593 773.1 0 593 284 123 773.1 0 123 285 183 773.1 0 183 286 191 773.1 0 191 287 491 773.1 0 491 288 438 773.1 0 438 289 854 773.1 0 854 290 651 773.1 0 651 291 492 773.1 0 492 292 227 773.1 0 227 293 132 773.1 0 132 294 838 773.1 0 838 295 698 773.1 0 698 296 704 773.1 0 704 297 201 773.1 0 201 298 116 773.1 0 116 299 136 773.1 0 136 300 92 773.1 0 92 301 290 773.1 0 290 Finished in 2.78 s (65 us/read; 0.92 M reads/minute). === Summary === Total reads processed: 42,707 Reads with adapters: 8,638 (20.2%) Reads that were too short: 38 (0.1%) Reads written (passing filters): 8,600 (20.1%) Total basepairs processed: 9,404,803 bp Total written (filtered): 2,342,991 bp (24.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8638 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 60 0.0 3 0 51 6 2 1 20 7987 0.0 4 7626 279 62 11 9 21 406 0.0 4 296 76 17 8 9 22 10 0.0 4 0 0 0 1 9 23 15 0.0 4 2 0 1 8 4 24 3 0.0 4 0 0 0 1 2 28 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 1 30 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 1 48 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 2 0.0 4 0 0 0 0 2 56 2 0.0 4 0 0 0 0 2 57 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 79 2 0.0 4 0 0 0 0 2 87 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 102 0.0 4 0 0 0 0 102 128 1 0.0 4 0 0 0 0 1 130 5 0.0 4 0 0 0 0 5 133 2 0.0 4 0 0 0 0 2 135 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 224 1 0.0 4 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 4 0.0 4 0 0 1 0 3 233 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 8,600 Reads with adapters: 655 (7.6%) Reads written (passing filters): 8,600 (100.0%) Total basepairs processed: 2,342,991 bp Total written (filtered): 2,335,431 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 655 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.9% C: 6.7% G: 86.4% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 33 134.4 0 33 4 4 33.6 0 4 9 5 0.0 0 1 4 10 11 0.0 1 0 11 11 548 0.0 1 6 542 12 45 0.0 1 2 43 13 3 0.0 1 0 3 15 1 0.0 1 0 1 19 2 0.0 1 2 210 3 0.0 1 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.