This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACGCGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 142.19 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 320,910 (10.1%) Reads that were too short: 288,323 (9.1%) Reads written (passing filters): 32,587 (1.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 7,883,706 bp (0.8%) === Adapter 1 === Sequence: ACGCGC; Type: regular 5'; Length: 6; Trimmed: 320910 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3009 773.1 0 3009 7 9495 773.1 0 9495 8 222 773.1 0 222 9 7879 773.1 0 7879 10 29 773.1 0 29 11 22 773.1 0 22 12 21 773.1 0 21 13 31 773.1 0 31 14 26 773.1 0 26 15 16 773.1 0 16 16 11 773.1 0 11 17 21 773.1 0 21 18 33 773.1 0 33 19 26 773.1 0 26 20 21 773.1 0 21 21 41 773.1 0 41 22 32 773.1 0 32 23 24 773.1 0 24 24 14 773.1 0 14 25 7 773.1 0 7 26 15 773.1 0 15 27 18 773.1 0 18 28 24 773.1 0 24 29 26 773.1 0 26 30 20 773.1 0 20 31 14 773.1 0 14 32 11 773.1 0 11 33 32 773.1 0 32 34 12 773.1 0 12 35 23 773.1 0 23 36 36 773.1 0 36 37 19 773.1 0 19 38 22 773.1 0 22 39 23 773.1 0 23 40 18 773.1 0 18 41 35 773.1 0 35 42 17 773.1 0 17 43 15 773.1 0 15 44 18 773.1 0 18 45 28 773.1 0 28 46 31 773.1 0 31 47 45 773.1 0 45 48 21 773.1 0 21 49 42 773.1 0 42 50 17 773.1 0 17 51 29 773.1 0 29 52 20 773.1 0 20 53 50 773.1 0 50 54 39 773.1 0 39 55 65 773.1 0 65 56 40 773.1 0 40 57 24 773.1 0 24 58 24 773.1 0 24 59 33 773.1 0 33 60 29 773.1 0 29 61 25 773.1 0 25 62 26 773.1 0 26 63 25 773.1 0 25 64 34 773.1 0 34 65 20 773.1 0 20 66 24 773.1 0 24 67 16 773.1 0 16 68 14 773.1 0 14 69 26 773.1 0 26 70 25 773.1 0 25 71 19 773.1 0 19 72 45 773.1 0 45 73 36 773.1 0 36 74 45 773.1 0 45 75 23 773.1 0 23 76 18 773.1 0 18 77 14 773.1 0 14 78 20 773.1 0 20 79 19 773.1 0 19 80 25 773.1 0 25 81 21 773.1 0 21 82 25 773.1 0 25 83 37 773.1 0 37 84 33 773.1 0 33 85 28 773.1 0 28 86 38 773.1 0 38 87 31 773.1 0 31 88 21 773.1 0 21 89 20 773.1 0 20 90 17 773.1 0 17 91 28 773.1 0 28 92 19 773.1 0 19 93 21 773.1 0 21 94 19 773.1 0 19 95 18 773.1 0 18 96 20 773.1 0 20 97 25 773.1 0 25 98 18 773.1 0 18 99 23 773.1 0 23 100 16 773.1 0 16 101 19 773.1 0 19 102 18 773.1 0 18 103 22 773.1 0 22 104 22 773.1 0 22 105 26 773.1 0 26 106 19 773.1 0 19 107 28 773.1 0 28 108 35 773.1 0 35 109 18 773.1 0 18 110 16 773.1 0 16 111 20 773.1 0 20 112 35 773.1 0 35 113 32 773.1 0 32 114 47 773.1 0 47 115 50 773.1 0 50 116 63 773.1 0 63 117 46 773.1 0 46 118 29 773.1 0 29 119 31 773.1 0 31 120 26 773.1 0 26 121 17 773.1 0 17 122 58 773.1 0 58 123 84 773.1 0 84 124 92 773.1 0 92 125 203 773.1 0 203 126 118 773.1 0 118 127 50 773.1 0 50 128 31 773.1 0 31 129 41 773.1 0 41 130 33 773.1 0 33 131 22 773.1 0 22 132 25 773.1 0 25 133 27 773.1 0 27 134 22 773.1 0 22 135 26 773.1 0 26 136 26 773.1 0 26 137 25 773.1 0 25 138 50 773.1 0 50 139 29 773.1 0 29 140 35 773.1 0 35 141 40 773.1 0 40 142 27 773.1 0 27 143 37 773.1 0 37 144 32 773.1 0 32 145 24 773.1 0 24 146 33 773.1 0 33 147 34 773.1 0 34 148 19 773.1 0 19 149 50 773.1 0 50 150 85 773.1 0 85 151 65 773.1 0 65 152 100 773.1 0 100 153 47 773.1 0 47 154 32 773.1 0 32 155 38 773.1 0 38 156 45 773.1 0 45 157 27 773.1 0 27 158 33 773.1 0 33 159 50 773.1 0 50 160 178 773.1 0 178 161 108 773.1 0 108 162 166 773.1 0 166 163 45 773.1 0 45 164 57 773.1 0 57 165 417 773.1 0 417 166 295 773.1 0 295 167 365 773.1 0 365 168 92 773.1 0 92 169 53 773.1 0 53 170 43 773.1 0 43 171 33 773.1 0 33 172 39 773.1 0 39 173 45 773.1 0 45 174 37 773.1 0 37 175 38 773.1 0 38 176 35 773.1 0 35 177 71 773.1 0 71 178 54 773.1 0 54 179 115 773.1 0 115 180 73 773.1 0 73 181 89 773.1 0 89 182 60 773.1 0 60 183 141 773.1 0 141 184 44 773.1 0 44 185 283 773.1 0 283 186 354 773.1 0 354 187 1384 773.1 0 1384 188 838 773.1 0 838 189 867 773.1 0 867 190 45 773.1 0 45 191 38 773.1 0 38 192 37 773.1 0 37 193 42 773.1 0 42 194 47 773.1 0 47 195 33 773.1 0 33 196 42 773.1 0 42 197 57 773.1 0 57 198 61 773.1 0 61 199 82 773.1 0 82 200 293 773.1 0 293 201 65 773.1 0 65 202 55 773.1 0 55 203 47 773.1 0 47 204 48 773.1 0 48 205 35 773.1 0 35 206 28 773.1 0 28 207 66 773.1 0 66 208 34 773.1 0 34 209 67 773.1 0 67 210 286 773.1 0 286 211 41 773.1 0 41 212 35 773.1 0 35 213 47 773.1 0 47 214 54 773.1 0 54 215 30 773.1 0 30 216 60 773.1 0 60 217 112 773.1 0 112 218 147 773.1 0 147 219 315 773.1 0 315 220 619 773.1 0 619 221 2345 773.1 0 2345 222 3252 773.1 0 3252 223 3506 773.1 0 3506 224 1984 773.1 0 1984 225 788 773.1 0 788 226 334 773.1 0 334 227 320 773.1 0 320 228 115 773.1 0 115 229 132 773.1 0 132 230 249 773.1 0 249 231 347 773.1 0 347 232 573 773.1 0 573 233 964 773.1 0 964 234 1105 773.1 0 1105 235 2387 773.1 0 2387 236 5337 773.1 0 5337 237 85663 773.1 0 85663 238 54001 773.1 0 54001 239 108238 773.1 0 108238 240 3522 773.1 0 3522 241 818 773.1 0 818 242 96 773.1 0 96 243 58 773.1 0 58 244 89 773.1 0 89 245 810 773.1 0 810 246 959 773.1 0 959 247 1700 773.1 0 1700 248 920 773.1 0 920 249 357 773.1 0 357 250 270 773.1 0 270 251 80 773.1 0 80 252 37 773.1 0 37 253 18 773.1 0 18 254 20 773.1 0 20 255 32 773.1 0 32 256 50 773.1 0 50 257 23 773.1 0 23 258 18 773.1 0 18 259 17 773.1 0 17 260 35 773.1 0 35 261 45 773.1 0 45 262 55 773.1 0 55 263 27 773.1 0 27 264 34 773.1 0 34 265 28 773.1 0 28 266 42 773.1 0 42 267 80 773.1 0 80 268 427 773.1 0 427 269 300 773.1 0 300 270 330 773.1 0 330 271 144 773.1 0 144 272 64 773.1 0 64 273 61 773.1 0 61 274 108 773.1 0 108 275 44 773.1 0 44 276 65 773.1 0 65 277 37 773.1 0 37 278 48 773.1 0 48 279 34 773.1 0 34 280 65 773.1 0 65 281 228 773.1 0 228 282 131 773.1 0 131 283 194 773.1 0 194 284 49 773.1 0 49 285 47 773.1 0 47 286 63 773.1 0 63 287 124 773.1 0 124 288 147 773.1 0 147 289 254 773.1 0 254 290 141 773.1 0 141 291 132 773.1 0 132 292 49 773.1 0 49 293 31 773.1 0 31 294 319 773.1 0 319 295 217 773.1 0 217 296 220 773.1 0 220 297 81 773.1 0 81 298 39 773.1 0 39 299 32 773.1 0 32 300 26 773.1 0 26 301 66 773.1 0 66 Finished in 2.14 s (66 us/read; 0.91 M reads/minute). === Summary === Total reads processed: 32,587 Reads with adapters: 10,627 (32.6%) Reads that were too short: 72 (0.2%) Reads written (passing filters): 10,555 (32.4%) Total basepairs processed: 7,883,706 bp Total written (filtered): 2,895,674 bp (36.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10627 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 27 0.0 3 0 25 2 17 8068 0.0 3 7867 192 9 18 74 0.0 3 52 20 2 19 18 0.0 3 1 13 4 20 2303 0.0 3 2252 43 1 7 21 12 0.0 3 1 3 0 8 22 1 0.0 3 0 1 24 2 0.0 3 0 0 0 2 32 38 0.0 3 0 0 0 38 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 4 0.0 3 0 0 0 4 58 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 66 4 0.0 3 0 0 0 4 74 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 173 1 0.0 3 1 184 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 43 0.0 3 0 0 0 43 224 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 270 1 0.0 3 1 275 1 0.0 3 0 0 1 277 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.57 s (54 us/read; 1.11 M reads/minute). === Summary === Total reads processed: 10,555 Reads with adapters: 520 (4.9%) Reads written (passing filters): 10,555 (100.0%) Total basepairs processed: 2,895,674 bp Total written (filtered): 2,894,109 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 520 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 81.2% C: 6.2% G: 12.1% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 515 164.9 0 515 4 5 41.2 0 5 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.