This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 144.36 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 494,908 (15.6%) Reads that were too short: 9,610 (0.3%) Reads written (passing filters): 485,298 (15.3%) Total basepairs processed: 953,096,634 bp Total written (filtered): 101,594,440 bp (10.7%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 494908 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1205 773.1 0 1205 7 89159 773.1 0 89159 8 6963 773.1 0 6963 9 74615 773.1 0 74615 10 143 773.1 0 143 11 37 773.1 0 37 12 51 773.1 0 51 13 83 773.1 0 83 14 80 773.1 0 80 15 79 773.1 0 79 16 52 773.1 0 52 17 30 773.1 0 30 18 39 773.1 0 39 19 55 773.1 0 55 20 44 773.1 0 44 21 52 773.1 0 52 22 29 773.1 0 29 23 39 773.1 0 39 24 55 773.1 0 55 25 70 773.1 0 70 26 116 773.1 0 116 27 759 773.1 0 759 28 1414 773.1 0 1414 29 803 773.1 0 803 30 36 773.1 0 36 31 53 773.1 0 53 32 43 773.1 0 43 33 59 773.1 0 59 34 70 773.1 0 70 35 58 773.1 0 58 36 41 773.1 0 41 37 68 773.1 0 68 38 31 773.1 0 31 39 52 773.1 0 52 40 43 773.1 0 43 41 49 773.1 0 49 42 72 773.1 0 72 43 64 773.1 0 64 44 69 773.1 0 69 45 55 773.1 0 55 46 57 773.1 0 57 47 55 773.1 0 55 48 55 773.1 0 55 49 52 773.1 0 52 50 75 773.1 0 75 51 94 773.1 0 94 52 61 773.1 0 61 53 56 773.1 0 56 54 41 773.1 0 41 55 42 773.1 0 42 56 60 773.1 0 60 57 184 773.1 0 184 58 298 773.1 0 298 59 563 773.1 0 563 60 63 773.1 0 63 61 38 773.1 0 38 62 47 773.1 0 47 63 53 773.1 0 53 64 47 773.1 0 47 65 70 773.1 0 70 66 40 773.1 0 40 67 53 773.1 0 53 68 67 773.1 0 67 69 51 773.1 0 51 70 69 773.1 0 69 71 75 773.1 0 75 72 37 773.1 0 37 73 55 773.1 0 55 74 67 773.1 0 67 75 58 773.1 0 58 76 61 773.1 0 61 77 70 773.1 0 70 78 60 773.1 0 60 79 49 773.1 0 49 80 72 773.1 0 72 81 154 773.1 0 154 82 55 773.1 0 55 83 60 773.1 0 60 84 78 773.1 0 78 85 59 773.1 0 59 86 48 773.1 0 48 87 43 773.1 0 43 88 51 773.1 0 51 89 71 773.1 0 71 90 104 773.1 0 104 91 214 773.1 0 214 92 128 773.1 0 128 93 132 773.1 0 132 94 133 773.1 0 133 95 171 773.1 0 171 96 295 773.1 0 295 97 103 773.1 0 103 98 103 773.1 0 103 99 89 773.1 0 89 100 85 773.1 0 85 101 148 773.1 0 148 102 203 773.1 0 203 103 314 773.1 0 314 104 328 773.1 0 328 105 512 773.1 0 512 106 1043 773.1 0 1043 107 2836 773.1 0 2836 108 40445 773.1 0 40445 109 34537 773.1 0 34537 110 27990 773.1 0 27990 111 314 773.1 0 314 112 73 773.1 0 73 113 61 773.1 0 61 114 48 773.1 0 48 115 62 773.1 0 62 116 114 773.1 0 114 117 125 773.1 0 125 118 80 773.1 0 80 119 98 773.1 0 98 120 120 773.1 0 120 121 194 773.1 0 194 122 105 773.1 0 105 123 107 773.1 0 107 124 99 773.1 0 99 125 119 773.1 0 119 126 90 773.1 0 90 127 250 773.1 0 250 128 250 773.1 0 250 129 361 773.1 0 361 130 158 773.1 0 158 131 227 773.1 0 227 132 202 773.1 0 202 133 274 773.1 0 274 134 3593 773.1 0 3593 135 4316 773.1 0 4316 136 7603 773.1 0 7603 137 219 773.1 0 219 138 99 773.1 0 99 139 79 773.1 0 79 140 138 773.1 0 138 141 205 773.1 0 205 142 270 773.1 0 270 143 195 773.1 0 195 144 238 773.1 0 238 145 242 773.1 0 242 146 308 773.1 0 308 147 552 773.1 0 552 148 176 773.1 0 176 149 291 773.1 0 291 150 420 773.1 0 420 151 233 773.1 0 233 152 255 773.1 0 255 153 457 773.1 0 457 154 803 773.1 0 803 155 2627 773.1 0 2627 156 1883 773.1 0 1883 157 2221 773.1 0 2221 158 1560 773.1 0 1560 159 1924 773.1 0 1924 160 38020 773.1 0 38020 161 71361 773.1 0 71361 162 48121 773.1 0 48121 163 257 773.1 0 257 164 120 773.1 0 120 165 94 773.1 0 94 166 87 773.1 0 87 167 68 773.1 0 68 168 40 773.1 0 40 169 107 773.1 0 107 170 110 773.1 0 110 171 54 773.1 0 54 172 45 773.1 0 45 173 54 773.1 0 54 174 87 773.1 0 87 175 99 773.1 0 99 176 61 773.1 0 61 177 58 773.1 0 58 178 52 773.1 0 52 179 98 773.1 0 98 180 101 773.1 0 101 181 98 773.1 0 98 182 81 773.1 0 81 183 85 773.1 0 85 184 56 773.1 0 56 185 58 773.1 0 58 186 66 773.1 0 66 187 63 773.1 0 63 188 53 773.1 0 53 189 43 773.1 0 43 190 70 773.1 0 70 191 153 773.1 0 153 192 102 773.1 0 102 193 147 773.1 0 147 194 61 773.1 0 61 195 54 773.1 0 54 196 55 773.1 0 55 197 61 773.1 0 61 198 51 773.1 0 51 199 55 773.1 0 55 200 42 773.1 0 42 201 50 773.1 0 50 202 57 773.1 0 57 203 85 773.1 0 85 204 91 773.1 0 91 205 66 773.1 0 66 206 66 773.1 0 66 207 59 773.1 0 59 208 68 773.1 0 68 209 67 773.1 0 67 210 70 773.1 0 70 211 51 773.1 0 51 212 58 773.1 0 58 213 39 773.1 0 39 214 49 773.1 0 49 215 48 773.1 0 48 216 45 773.1 0 45 217 52 773.1 0 52 218 66 773.1 0 66 219 83 773.1 0 83 220 87 773.1 0 87 221 107 773.1 0 107 222 259 773.1 0 259 223 303 773.1 0 303 224 144 773.1 0 144 225 170 773.1 0 170 226 61 773.1 0 61 227 54 773.1 0 54 228 49 773.1 0 49 229 49 773.1 0 49 230 54 773.1 0 54 231 62 773.1 0 62 232 99 773.1 0 99 233 67 773.1 0 67 234 116 773.1 0 116 235 113 773.1 0 113 236 158 773.1 0 158 237 148 773.1 0 148 238 70 773.1 0 70 239 54 773.1 0 54 240 62 773.1 0 62 241 77 773.1 0 77 242 85 773.1 0 85 243 72 773.1 0 72 244 49 773.1 0 49 245 45 773.1 0 45 246 45 773.1 0 45 247 38 773.1 0 38 248 58 773.1 0 58 249 62 773.1 0 62 250 134 773.1 0 134 251 245 773.1 0 245 252 254 773.1 0 254 253 395 773.1 0 395 254 634 773.1 0 634 255 300 773.1 0 300 256 252 773.1 0 252 257 71 773.1 0 71 258 74 773.1 0 74 259 42 773.1 0 42 260 56 773.1 0 56 261 112 773.1 0 112 262 107 773.1 0 107 263 110 773.1 0 110 264 65 773.1 0 65 265 81 773.1 0 81 266 53 773.1 0 53 267 46 773.1 0 46 268 48 773.1 0 48 269 64 773.1 0 64 270 88 773.1 0 88 271 100 773.1 0 100 272 102 773.1 0 102 273 51 773.1 0 51 274 54 773.1 0 54 275 57 773.1 0 57 276 51 773.1 0 51 277 74 773.1 0 74 278 121 773.1 0 121 279 113 773.1 0 113 280 119 773.1 0 119 281 106 773.1 0 106 282 177 773.1 0 177 283 181 773.1 0 181 284 119 773.1 0 119 285 58 773.1 0 58 286 44 773.1 0 44 287 42 773.1 0 42 288 29 773.1 0 29 289 49 773.1 0 49 290 76 773.1 0 76 291 82 773.1 0 82 292 83 773.1 0 83 293 72 773.1 0 72 294 51 773.1 0 51 295 62 773.1 0 62 296 68 773.1 0 68 297 64 773.1 0 64 298 41 773.1 0 41 299 76 773.1 0 76 300 61 773.1 0 61 301 59 773.1 0 59 Finished in 29.39 s (61 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 485,298 Reads with adapters: 82,733 (17.0%) Reads that were too short: 84 (0.0%) Reads written (passing filters): 82,649 (17.0%) Total basepairs processed: 101,594,440 bp Total written (filtered): 22,468,784 bp (22.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 82733 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 8 0.0 3 0 0 0 3 5 18 30 0.0 3 0 0 10 12 8 19 372 0.0 3 0 252 44 48 28 20 81530 0.0 4 77195 3449 620 187 79 21 209 0.0 4 18 171 9 7 4 22 22 0.0 4 0 0 15 5 2 23 283 0.0 4 1 1 0 192 89 24 6 0.0 4 1 0 0 0 5 29 1 0.0 4 0 0 1 35 1 0.0 4 0 0 0 0 1 39 2 0.0 4 2 42 1 0.0 4 1 54 1 0.0 4 0 0 0 0 1 55 3 0.0 4 0 0 0 0 3 61 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 74 1 0.0 4 1 77 4 0.0 4 0 0 0 0 4 80 4 0.0 4 0 0 0 0 4 81 3 0.0 4 0 0 0 0 3 82 3 0.0 4 0 0 0 0 3 91 2 0.0 4 0 0 0 0 2 92 2 0.0 4 0 0 0 0 2 96 1 0.0 4 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 124 2 0.0 4 1 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 163 0.0 4 0 0 0 0 163 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 1 143 1 0.0 4 1 145 1 0.0 4 0 0 0 1 152 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 155 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 166 3 0.0 4 0 0 0 0 3 207 1 0.0 4 0 0 0 0 1 216 2 0.0 4 0 0 0 0 2 221 2 0.0 4 0 0 0 0 2 222 1 0.0 4 0 0 0 0 1 228 9 0.0 4 0 0 0 0 9 229 6 0.0 4 0 0 0 1 5 230 1 0.0 4 0 0 0 0 1 231 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 235 5 0.0 4 0 0 0 0 5 237 4 0.0 4 0 0 0 0 4 238 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 265 2 0.0 4 0 0 0 0 2 277 2 0.0 4 0 0 0 1 1 282 3 0.0 4 0 0 0 0 3 283 2 0.0 4 0 0 0 0 2 292 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.91 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 82,649 Reads with adapters: 21,105 (25.5%) Reads written (passing filters): 82,649 (100.0%) Total basepairs processed: 22,468,784 bp Total written (filtered): 22,255,882 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 21105 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 5.7% G: 91.0% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 39 1291.4 0 39 4 17 322.8 0 17 5 2 80.7 0 2 6 3 20.2 0 3 7 7 5.0 0 7 8 11 1.3 0 11 9 8801 0.3 0 549 8252 10 8954 0.1 1 600 8354 11 3160 0.0 1 57 3103 12 29 0.0 1 0 29 13 25 0.0 1 4 21 14 5 0.0 1 0 5 17 3 0.0 1 3 19 1 0.0 1 0 1 22 1 0.0 1 1 23 1 0.0 1 1 50 1 0.0 1 1 71 1 0.0 1 1 104 1 0.0 1 0 1 115 1 0.0 1 1 120 3 0.0 1 3 121 1 0.0 1 1 150 4 0.0 1 3 1 156 5 0.0 1 3 2 161 1 0.0 1 1 189 1 0.0 1 0 1 208 21 0.0 1 14 7 209 4 0.0 1 3 1 210 1 0.0 1 1 220 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.