This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 145.84 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 462,801 (14.6%) Reads that were too short: 6,463 (0.2%) Reads written (passing filters): 456,338 (14.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 98,740,283 bp (10.4%) === Adapter 1 === Sequence: CCTAAT; Type: regular 5'; Length: 6; Trimmed: 462801 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 928 773.1 0 928 7 86864 773.1 0 86864 8 5521 773.1 0 5521 9 84651 773.1 0 84651 10 142 773.1 0 142 11 30 773.1 0 30 12 36 773.1 0 36 13 64 773.1 0 64 14 60 773.1 0 60 15 88 773.1 0 88 16 29 773.1 0 29 17 25 773.1 0 25 18 32 773.1 0 32 19 39 773.1 0 39 20 38 773.1 0 38 21 59 773.1 0 59 22 40 773.1 0 40 23 39 773.1 0 39 24 36 773.1 0 36 25 37 773.1 0 37 26 54 773.1 0 54 27 176 773.1 0 176 28 228 773.1 0 228 29 139 773.1 0 139 30 36 773.1 0 36 31 28 773.1 0 28 32 39 773.1 0 39 33 40 773.1 0 40 34 45 773.1 0 45 35 41 773.1 0 41 36 37 773.1 0 37 37 54 773.1 0 54 38 29 773.1 0 29 39 37 773.1 0 37 40 38 773.1 0 38 41 50 773.1 0 50 42 41 773.1 0 41 43 51 773.1 0 51 44 35 773.1 0 35 45 52 773.1 0 52 46 38 773.1 0 38 47 46 773.1 0 46 48 29 773.1 0 29 49 43 773.1 0 43 50 81 773.1 0 81 51 78 773.1 0 78 52 46 773.1 0 46 53 51 773.1 0 51 54 28 773.1 0 28 55 39 773.1 0 39 56 57 773.1 0 57 57 147 773.1 0 147 58 217 773.1 0 217 59 258 773.1 0 258 60 49 773.1 0 49 61 34 773.1 0 34 62 29 773.1 0 29 63 60 773.1 0 60 64 53 773.1 0 53 65 61 773.1 0 61 66 36 773.1 0 36 67 45 773.1 0 45 68 51 773.1 0 51 69 34 773.1 0 34 70 46 773.1 0 46 71 70 773.1 0 70 72 44 773.1 0 44 73 46 773.1 0 46 74 58 773.1 0 58 75 35 773.1 0 35 76 35 773.1 0 35 77 44 773.1 0 44 78 59 773.1 0 59 79 47 773.1 0 47 80 39 773.1 0 39 81 157 773.1 0 157 82 44 773.1 0 44 83 56 773.1 0 56 84 60 773.1 0 60 85 49 773.1 0 49 86 57 773.1 0 57 87 47 773.1 0 47 88 59 773.1 0 59 89 71 773.1 0 71 90 122 773.1 0 122 91 214 773.1 0 214 92 120 773.1 0 120 93 128 773.1 0 128 94 107 773.1 0 107 95 147 773.1 0 147 96 294 773.1 0 294 97 102 773.1 0 102 98 69 773.1 0 69 99 75 773.1 0 75 100 70 773.1 0 70 101 148 773.1 0 148 102 173 773.1 0 173 103 273 773.1 0 273 104 330 773.1 0 330 105 493 773.1 0 493 106 1094 773.1 0 1094 107 2495 773.1 0 2495 108 46741 773.1 0 46741 109 39882 773.1 0 39882 110 32577 773.1 0 32577 111 362 773.1 0 362 112 47 773.1 0 47 113 35 773.1 0 35 114 24 773.1 0 24 115 57 773.1 0 57 116 101 773.1 0 101 117 70 773.1 0 70 118 80 773.1 0 80 119 77 773.1 0 77 120 97 773.1 0 97 121 178 773.1 0 178 122 73 773.1 0 73 123 67 773.1 0 67 124 78 773.1 0 78 125 76 773.1 0 76 126 66 773.1 0 66 127 169 773.1 0 169 128 164 773.1 0 164 129 205 773.1 0 205 130 106 773.1 0 106 131 159 773.1 0 159 132 146 773.1 0 146 133 186 773.1 0 186 134 3190 773.1 0 3190 135 3811 773.1 0 3811 136 7023 773.1 0 7023 137 112 773.1 0 112 138 74 773.1 0 74 139 50 773.1 0 50 140 90 773.1 0 90 141 69 773.1 0 69 142 108 773.1 0 108 143 110 773.1 0 110 144 159 773.1 0 159 145 175 773.1 0 175 146 233 773.1 0 233 147 417 773.1 0 417 148 147 773.1 0 147 149 205 773.1 0 205 150 347 773.1 0 347 151 185 773.1 0 185 152 196 773.1 0 196 153 386 773.1 0 386 154 634 773.1 0 634 155 2964 773.1 0 2964 156 1996 773.1 0 1996 157 2247 773.1 0 2247 158 973 773.1 0 973 159 1153 773.1 0 1153 160 27207 773.1 0 27207 161 53469 773.1 0 53469 162 35554 773.1 0 35554 163 152 773.1 0 152 164 70 773.1 0 70 165 59 773.1 0 59 166 83 773.1 0 83 167 45 773.1 0 45 168 36 773.1 0 36 169 82 773.1 0 82 170 117 773.1 0 117 171 38 773.1 0 38 172 30 773.1 0 30 173 30 773.1 0 30 174 46 773.1 0 46 175 58 773.1 0 58 176 29 773.1 0 29 177 45 773.1 0 45 178 37 773.1 0 37 179 45 773.1 0 45 180 81 773.1 0 81 181 69 773.1 0 69 182 32 773.1 0 32 183 44 773.1 0 44 184 42 773.1 0 42 185 40 773.1 0 40 186 31 773.1 0 31 187 42 773.1 0 42 188 32 773.1 0 32 189 30 773.1 0 30 190 42 773.1 0 42 191 103 773.1 0 103 192 76 773.1 0 76 193 94 773.1 0 94 194 43 773.1 0 43 195 30 773.1 0 30 196 27 773.1 0 27 197 35 773.1 0 35 198 29 773.1 0 29 199 33 773.1 0 33 200 37 773.1 0 37 201 42 773.1 0 42 202 46 773.1 0 46 203 51 773.1 0 51 204 69 773.1 0 69 205 46 773.1 0 46 206 38 773.1 0 38 207 30 773.1 0 30 208 72 773.1 0 72 209 41 773.1 0 41 210 37 773.1 0 37 211 25 773.1 0 25 212 29 773.1 0 29 213 32 773.1 0 32 214 28 773.1 0 28 215 41 773.1 0 41 216 43 773.1 0 43 217 52 773.1 0 52 218 64 773.1 0 64 219 41 773.1 0 41 220 51 773.1 0 51 221 71 773.1 0 71 222 189 773.1 0 189 223 241 773.1 0 241 224 95 773.1 0 95 225 100 773.1 0 100 226 37 773.1 0 37 227 45 773.1 0 45 228 40 773.1 0 40 229 30 773.1 0 30 230 42 773.1 0 42 231 47 773.1 0 47 232 60 773.1 0 60 233 55 773.1 0 55 234 89 773.1 0 89 235 92 773.1 0 92 236 125 773.1 0 125 237 145 773.1 0 145 238 41 773.1 0 41 239 35 773.1 0 35 240 27 773.1 0 27 241 37 773.1 0 37 242 52 773.1 0 52 243 37 773.1 0 37 244 33 773.1 0 33 245 37 773.1 0 37 246 14 773.1 0 14 247 38 773.1 0 38 248 31 773.1 0 31 249 45 773.1 0 45 250 111 773.1 0 111 251 259 773.1 0 259 252 234 773.1 0 234 253 293 773.1 0 293 254 291 773.1 0 291 255 94 773.1 0 94 256 95 773.1 0 95 257 27 773.1 0 27 258 61 773.1 0 61 259 33 773.1 0 33 260 46 773.1 0 46 261 78 773.1 0 78 262 85 773.1 0 85 263 79 773.1 0 79 264 38 773.1 0 38 265 39 773.1 0 39 266 32 773.1 0 32 267 20 773.1 0 20 268 33 773.1 0 33 269 38 773.1 0 38 270 61 773.1 0 61 271 47 773.1 0 47 272 68 773.1 0 68 273 41 773.1 0 41 274 38 773.1 0 38 275 46 773.1 0 46 276 39 773.1 0 39 277 62 773.1 0 62 278 92 773.1 0 92 279 98 773.1 0 98 280 71 773.1 0 71 281 62 773.1 0 62 282 88 773.1 0 88 283 112 773.1 0 112 284 85 773.1 0 85 285 51 773.1 0 51 286 43 773.1 0 43 287 26 773.1 0 26 288 32 773.1 0 32 289 31 773.1 0 31 290 47 773.1 0 47 291 58 773.1 0 58 292 62 773.1 0 62 293 38 773.1 0 38 294 33 773.1 0 33 295 40 773.1 0 40 296 45 773.1 0 45 297 37 773.1 0 37 298 27 773.1 0 27 299 43 773.1 0 43 300 39 773.1 0 39 301 49 773.1 0 49 Finished in 26.54 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 456,338 Reads with adapters: 87,965 (19.3%) Reads that were too short: 423 (0.1%) Reads written (passing filters): 87,542 (19.2%) Total basepairs processed: 98,740,283 bp Total written (filtered): 24,088,053 bp (24.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 87965 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 266 0.0 3 0 257 7 2 17 86059 0.0 3 84691 1319 44 5 18 150 0.0 3 10 137 3 19 33 0.0 3 0 0 30 3 20 65 0.0 3 0 0 0 65 21 1 0.0 3 1 29 1 0.0 3 0 0 0 1 30 2 0.0 3 0 0 0 2 31 852 0.0 3 0 0 4 848 34 72 0.0 3 0 0 1 71 38 1 0.0 3 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 57 12 0.0 3 0 0 0 12 60 2 0.0 3 0 0 0 2 63 5 0.0 3 0 0 0 5 64 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 91 2 0.0 3 0 0 0 2 104 7 0.0 3 0 0 0 7 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 4 0.0 3 0 0 1 3 113 1 0.0 3 0 0 0 1 114 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 1 123 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 132 21 0.0 3 0 0 0 21 133 1 0.0 3 0 0 0 1 134 2 0.0 3 1 0 0 1 135 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 3 0.0 3 0 0 0 3 159 6 0.0 3 0 0 0 6 161 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 0 3 192 3 0.0 3 0 0 0 3 194 4 0.0 3 0 0 0 4 195 2 0.0 3 0 0 0 2 204 1 0.0 3 0 0 0 1 216 3 0.0 3 0 0 0 3 217 336 0.0 3 0 0 8 328 218 3 0.0 3 0 0 0 3 240 2 0.0 3 1 0 0 1 246 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.61 s (53 us/read; 1.14 M reads/minute). === Summary === Total reads processed: 87,542 Reads with adapters: 117 (0.1%) Reads written (passing filters): 87,542 (100.0%) Total basepairs processed: 24,088,053 bp Total written (filtered): 24,087,679 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 117 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 27.4% C: 35.9% G: 19.7% T: 17.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 102 1367.8 0 102 4 9 342.0 0 9 5 4 85.5 0 4 6 2 21.4 0 2