This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 139.05 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 152,471 (4.8%) Reads that were too short: 17,802 (0.6%) Reads written (passing filters): 134,669 (4.3%) Total basepairs processed: 953,096,634 bp Total written (filtered): 35,799,151 bp (3.8%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 152471 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3872 773.1 0 3872 7 41499 773.1 0 41499 8 34464 773.1 0 34464 9 127 773.1 0 127 10 353 773.1 0 353 11 30 773.1 0 30 12 37 773.1 0 37 13 34 773.1 0 34 14 25 773.1 0 25 15 12 773.1 0 12 16 14 773.1 0 14 17 15 773.1 0 15 18 38 773.1 0 38 19 23 773.1 0 23 20 22 773.1 0 22 21 43 773.1 0 43 22 43 773.1 0 43 23 22 773.1 0 22 24 43 773.1 0 43 25 28 773.1 0 28 26 24 773.1 0 24 27 23 773.1 0 23 28 52 773.1 0 52 29 17 773.1 0 17 30 27 773.1 0 27 31 40 773.1 0 40 32 31 773.1 0 31 33 32 773.1 0 32 34 165 773.1 0 165 35 17 773.1 0 17 36 19 773.1 0 19 37 44 773.1 0 44 38 74 773.1 0 74 39 34 773.1 0 34 40 43 773.1 0 43 41 46 773.1 0 46 42 44 773.1 0 44 43 82 773.1 0 82 44 32 773.1 0 32 45 30 773.1 0 30 46 34 773.1 0 34 47 32 773.1 0 32 48 83 773.1 0 83 49 94 773.1 0 94 50 151 773.1 0 151 51 229 773.1 0 229 52 246 773.1 0 246 53 436 773.1 0 436 54 923 773.1 0 923 55 14809 773.1 0 14809 56 9784 773.1 0 9784 57 11600 773.1 0 11600 58 84 773.1 0 84 59 18 773.1 0 18 60 31 773.1 0 31 61 36 773.1 0 36 62 29 773.1 0 29 63 44 773.1 0 44 64 19 773.1 0 19 65 55 773.1 0 55 66 26 773.1 0 26 67 24 773.1 0 24 68 25 773.1 0 25 69 82 773.1 0 82 70 69 773.1 0 69 71 102 773.1 0 102 72 33 773.1 0 33 73 41 773.1 0 41 74 49 773.1 0 49 75 38 773.1 0 38 76 88 773.1 0 88 77 56 773.1 0 56 78 44 773.1 0 44 79 33 773.1 0 33 80 27 773.1 0 27 81 44 773.1 0 44 82 38 773.1 0 38 83 48 773.1 0 48 84 63 773.1 0 63 85 669 773.1 0 669 86 503 773.1 0 503 87 474 773.1 0 474 88 73 773.1 0 73 89 68 773.1 0 68 90 31 773.1 0 31 91 33 773.1 0 33 92 41 773.1 0 41 93 47 773.1 0 47 94 23 773.1 0 23 95 34 773.1 0 34 96 35 773.1 0 35 97 26 773.1 0 26 98 36 773.1 0 36 99 41 773.1 0 41 100 63 773.1 0 63 101 70 773.1 0 70 102 419 773.1 0 419 103 137 773.1 0 137 104 350 773.1 0 350 105 44 773.1 0 44 106 39 773.1 0 39 107 35 773.1 0 35 108 41 773.1 0 41 109 48 773.1 0 48 110 26 773.1 0 26 111 50 773.1 0 50 112 78 773.1 0 78 113 32 773.1 0 32 114 40 773.1 0 40 115 39 773.1 0 39 116 38 773.1 0 38 117 44 773.1 0 44 118 414 773.1 0 414 119 353 773.1 0 353 120 215 773.1 0 215 121 34 773.1 0 34 122 46 773.1 0 46 123 36 773.1 0 36 124 63 773.1 0 63 125 50 773.1 0 50 126 93 773.1 0 93 127 53 773.1 0 53 128 99 773.1 0 99 129 32 773.1 0 32 130 48 773.1 0 48 131 53 773.1 0 53 132 93 773.1 0 93 133 123 773.1 0 123 134 77 773.1 0 77 135 72 773.1 0 72 136 48 773.1 0 48 137 283 773.1 0 283 138 501 773.1 0 501 139 579 773.1 0 579 140 151 773.1 0 151 141 61 773.1 0 61 142 52 773.1 0 52 143 43 773.1 0 43 144 43 773.1 0 43 145 69 773.1 0 69 146 115 773.1 0 115 147 97 773.1 0 97 148 100 773.1 0 100 149 40 773.1 0 40 150 74 773.1 0 74 151 47 773.1 0 47 152 50 773.1 0 50 153 47 773.1 0 47 154 52 773.1 0 52 155 43 773.1 0 43 156 196 773.1 0 196 157 173 773.1 0 173 158 165 773.1 0 165 159 152 773.1 0 152 160 43 773.1 0 43 161 74 773.1 0 74 162 104 773.1 0 104 163 80 773.1 0 80 164 128 773.1 0 128 165 88 773.1 0 88 166 74 773.1 0 74 167 335 773.1 0 335 168 233 773.1 0 233 169 279 773.1 0 279 170 64 773.1 0 64 171 36 773.1 0 36 172 40 773.1 0 40 173 39 773.1 0 39 174 50 773.1 0 50 175 36 773.1 0 36 176 77 773.1 0 77 177 48 773.1 0 48 178 39 773.1 0 39 179 33 773.1 0 33 180 39 773.1 0 39 181 82 773.1 0 82 182 85 773.1 0 85 183 167 773.1 0 167 184 153 773.1 0 153 185 97 773.1 0 97 186 65 773.1 0 65 187 353 773.1 0 353 188 378 773.1 0 378 189 347 773.1 0 347 190 184 773.1 0 184 191 54 773.1 0 54 192 69 773.1 0 69 193 28 773.1 0 28 194 55 773.1 0 55 195 63 773.1 0 63 196 44 773.1 0 44 197 36 773.1 0 36 198 81 773.1 0 81 199 79 773.1 0 79 200 105 773.1 0 105 201 56 773.1 0 56 202 42 773.1 0 42 203 59 773.1 0 59 204 40 773.1 0 40 205 53 773.1 0 53 206 80 773.1 0 80 207 77 773.1 0 77 208 63 773.1 0 63 209 49 773.1 0 49 210 39 773.1 0 39 211 29 773.1 0 29 212 45 773.1 0 45 213 50 773.1 0 50 214 51 773.1 0 51 215 101 773.1 0 101 216 53 773.1 0 53 217 71 773.1 0 71 218 159 773.1 0 159 219 1921 773.1 0 1921 220 1130 773.1 0 1130 221 1596 773.1 0 1596 222 46 773.1 0 46 223 52 773.1 0 52 224 40 773.1 0 40 225 53 773.1 0 53 226 40 773.1 0 40 227 33 773.1 0 33 228 51 773.1 0 51 229 53 773.1 0 53 230 22 773.1 0 22 231 39 773.1 0 39 232 44 773.1 0 44 233 49 773.1 0 49 234 49 773.1 0 49 235 87 773.1 0 87 236 69 773.1 0 69 237 37 773.1 0 37 238 66 773.1 0 66 239 117 773.1 0 117 240 142 773.1 0 142 241 118 773.1 0 118 242 41 773.1 0 41 243 52 773.1 0 52 244 51 773.1 0 51 245 63 773.1 0 63 246 38 773.1 0 38 247 66 773.1 0 66 248 41 773.1 0 41 249 76 773.1 0 76 250 96 773.1 0 96 251 125 773.1 0 125 252 83 773.1 0 83 253 50 773.1 0 50 254 40 773.1 0 40 255 33 773.1 0 33 256 48 773.1 0 48 257 145 773.1 0 145 258 177 773.1 0 177 259 361 773.1 0 361 260 481 773.1 0 481 261 427 773.1 0 427 262 321 773.1 0 321 263 39 773.1 0 39 264 47 773.1 0 47 265 54 773.1 0 54 266 75 773.1 0 75 267 107 773.1 0 107 268 104 773.1 0 104 269 141 773.1 0 141 270 62 773.1 0 62 271 34 773.1 0 34 272 54 773.1 0 54 273 52 773.1 0 52 274 95 773.1 0 95 275 77 773.1 0 77 276 164 773.1 0 164 277 190 773.1 0 190 278 414 773.1 0 414 279 232 773.1 0 232 280 341 773.1 0 341 281 157 773.1 0 157 282 114 773.1 0 114 283 176 773.1 0 176 284 120 773.1 0 120 285 139 773.1 0 139 286 76 773.1 0 76 287 286 773.1 0 286 288 295 773.1 0 295 289 276 773.1 0 276 290 86 773.1 0 86 291 123 773.1 0 123 292 127 773.1 0 127 293 137 773.1 0 137 294 149 773.1 0 149 295 116 773.1 0 116 296 231 773.1 0 231 297 221 773.1 0 221 298 681 773.1 0 681 299 914 773.1 0 914 300 1196 773.1 0 1196 301 170 773.1 0 170 Finished in 9.61 s (71 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 134,669 Reads with adapters: 38,407 (28.5%) Reads that were too short: 44 (0.0%) Reads written (passing filters): 38,363 (28.5%) Total basepairs processed: 35,799,151 bp Total written (filtered): 10,462,760 bp (29.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 38407 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 8 0.0 3 0 0 6 2 19 175 0.0 3 0 121 23 27 4 20 34807 0.0 4 33041 1443 242 63 18 21 69 0.0 4 15 45 3 6 22 34 0.0 4 8 10 6 6 4 23 3162 0.0 4 2893 152 32 50 35 24 8 0.0 4 2 4 1 0 1 25 2 0.0 4 0 0 1 1 26 15 0.0 4 0 0 0 10 5 36 1 0.0 4 1 37 1 0.0 4 0 0 0 1 44 1 0.0 4 1 45 2 0.0 4 0 0 0 0 2 46 2 0.0 4 1 1 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 1 50 2 0.0 4 1 0 0 0 1 54 2 0.0 4 0 0 0 0 2 59 14 0.0 4 0 0 0 0 14 74 2 0.0 4 1 0 0 0 1 77 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 127 48 0.0 4 1 0 0 0 47 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 1 169 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 3 0.0 4 0 0 0 0 3 264 1 0.0 4 0 0 0 0 1 282 4 0.0 4 0 0 0 0 4 284 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 294 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.16 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 38,363 Reads with adapters: 7,671 (20.0%) Reads written (passing filters): 38,363 (100.0%) Total basepairs processed: 10,462,760 bp Total written (filtered): 10,379,887 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 7671 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 6.3% G: 90.4% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 34 599.4 0 34 4 13 149.9 0 13 5 2 37.5 0 2 7 2 2.3 0 2 8 2 0.6 0 2 9 423 0.1 0 27 396 10 2776 0.0 1 156 2620 11 4366 0.0 1 103 4263 12 22 0.0 1 1 21 13 11 0.0 1 0 11 14 4 0.0 1 0 4 122 1 0.0 1 0 1 151 10 0.0 1 4 6 156 1 0.0 1 1 208 1 0.0 1 1 209 3 0.0 1 2 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.