This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 139.84 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 149,649 (4.7%) Reads that were too short: 11,079 (0.3%) Reads written (passing filters): 138,570 (4.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 37,417,236 bp (3.9%) === Adapter 1 === Sequence: GTGCCT; Type: regular 5'; Length: 6; Trimmed: 149649 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4235 773.1 0 4235 7 35473 773.1 0 35473 8 43445 773.1 0 43445 9 112 773.1 0 112 10 32 773.1 0 32 11 28 773.1 0 28 12 10 773.1 0 10 13 16 773.1 0 16 14 10 773.1 0 10 15 10 773.1 0 10 16 11 773.1 0 11 17 28 773.1 0 28 18 43 773.1 0 43 19 21 773.1 0 21 20 18 773.1 0 18 21 26 773.1 0 26 22 18 773.1 0 18 23 17 773.1 0 17 24 28 773.1 0 28 25 27 773.1 0 27 26 18 773.1 0 18 27 41 773.1 0 41 28 75 773.1 0 75 29 15 773.1 0 15 30 20 773.1 0 20 31 23 773.1 0 23 32 12 773.1 0 12 33 18 773.1 0 18 34 164 773.1 0 164 35 15 773.1 0 15 36 12 773.1 0 12 37 37 773.1 0 37 38 85 773.1 0 85 39 18 773.1 0 18 40 44 773.1 0 44 41 44 773.1 0 44 42 39 773.1 0 39 43 81 773.1 0 81 44 43 773.1 0 43 45 33 773.1 0 33 46 23 773.1 0 23 47 43 773.1 0 43 48 74 773.1 0 74 49 99 773.1 0 99 50 120 773.1 0 120 51 215 773.1 0 215 52 231 773.1 0 231 53 476 773.1 0 476 54 915 773.1 0 915 55 17680 773.1 0 17680 56 11705 773.1 0 11705 57 14145 773.1 0 14145 58 64 773.1 0 64 59 19 773.1 0 19 60 18 773.1 0 18 61 24 773.1 0 24 62 30 773.1 0 30 63 28 773.1 0 28 64 12 773.1 0 12 65 25 773.1 0 25 66 19 773.1 0 19 67 12 773.1 0 12 68 17 773.1 0 17 69 43 773.1 0 43 70 34 773.1 0 34 71 46 773.1 0 46 72 27 773.1 0 27 73 27 773.1 0 27 74 23 773.1 0 23 75 23 773.1 0 23 76 36 773.1 0 36 77 37 773.1 0 37 78 27 773.1 0 27 79 17 773.1 0 17 80 17 773.1 0 17 81 29 773.1 0 29 82 35 773.1 0 35 83 35 773.1 0 35 84 46 773.1 0 46 85 602 773.1 0 602 86 553 773.1 0 553 87 404 773.1 0 404 88 29 773.1 0 29 89 20 773.1 0 20 90 20 773.1 0 20 91 18 773.1 0 18 92 21 773.1 0 21 93 36 773.1 0 36 94 18 773.1 0 18 95 15 773.1 0 15 96 20 773.1 0 20 97 19 773.1 0 19 98 20 773.1 0 20 99 21 773.1 0 21 100 32 773.1 0 32 101 30 773.1 0 30 102 113 773.1 0 113 103 83 773.1 0 83 104 184 773.1 0 184 105 28 773.1 0 28 106 28 773.1 0 28 107 24 773.1 0 24 108 33 773.1 0 33 109 30 773.1 0 30 110 21 773.1 0 21 111 15 773.1 0 15 112 66 773.1 0 66 113 22 773.1 0 22 114 14 773.1 0 14 115 12 773.1 0 12 116 22 773.1 0 22 117 29 773.1 0 29 118 267 773.1 0 267 119 196 773.1 0 196 120 73 773.1 0 73 121 17 773.1 0 17 122 26 773.1 0 26 123 20 773.1 0 20 124 25 773.1 0 25 125 13 773.1 0 13 126 53 773.1 0 53 127 28 773.1 0 28 128 74 773.1 0 74 129 37 773.1 0 37 130 34 773.1 0 34 131 29 773.1 0 29 132 44 773.1 0 44 133 25 773.1 0 25 134 27 773.1 0 27 135 29 773.1 0 29 136 34 773.1 0 34 137 122 773.1 0 122 138 279 773.1 0 279 139 284 773.1 0 284 140 83 773.1 0 83 141 55 773.1 0 55 142 26 773.1 0 26 143 32 773.1 0 32 144 19 773.1 0 19 145 34 773.1 0 34 146 71 773.1 0 71 147 52 773.1 0 52 148 43 773.1 0 43 149 20 773.1 0 20 150 56 773.1 0 56 151 28 773.1 0 28 152 21 773.1 0 21 153 31 773.1 0 31 154 27 773.1 0 27 155 31 773.1 0 31 156 211 773.1 0 211 157 80 773.1 0 80 158 75 773.1 0 75 159 78 773.1 0 78 160 17 773.1 0 17 161 39 773.1 0 39 162 44 773.1 0 44 163 53 773.1 0 53 164 50 773.1 0 50 165 32 773.1 0 32 166 37 773.1 0 37 167 95 773.1 0 95 168 104 773.1 0 104 169 143 773.1 0 143 170 24 773.1 0 24 171 11 773.1 0 11 172 25 773.1 0 25 173 32 773.1 0 32 174 31 773.1 0 31 175 25 773.1 0 25 176 62 773.1 0 62 177 18 773.1 0 18 178 18 773.1 0 18 179 14 773.1 0 14 180 18 773.1 0 18 181 29 773.1 0 29 182 35 773.1 0 35 183 100 773.1 0 100 184 72 773.1 0 72 185 72 773.1 0 72 186 29 773.1 0 29 187 277 773.1 0 277 188 102 773.1 0 102 189 137 773.1 0 137 190 89 773.1 0 89 191 41 773.1 0 41 192 54 773.1 0 54 193 14 773.1 0 14 194 35 773.1 0 35 195 31 773.1 0 31 196 11 773.1 0 11 197 19 773.1 0 19 198 44 773.1 0 44 199 64 773.1 0 64 200 40 773.1 0 40 201 31 773.1 0 31 202 32 773.1 0 32 203 30 773.1 0 30 204 18 773.1 0 18 205 30 773.1 0 30 206 35 773.1 0 35 207 35 773.1 0 35 208 29 773.1 0 29 209 59 773.1 0 59 210 25 773.1 0 25 211 13 773.1 0 13 212 21 773.1 0 21 213 19 773.1 0 19 214 22 773.1 0 22 215 51 773.1 0 51 216 31 773.1 0 31 217 52 773.1 0 52 218 63 773.1 0 63 219 982 773.1 0 982 220 643 773.1 0 643 221 804 773.1 0 804 222 14 773.1 0 14 223 10 773.1 0 10 224 18 773.1 0 18 225 22 773.1 0 22 226 36 773.1 0 36 227 21 773.1 0 21 228 27 773.1 0 27 229 25 773.1 0 25 230 18 773.1 0 18 231 31 773.1 0 31 232 27 773.1 0 27 233 20 773.1 0 20 234 20 773.1 0 20 235 30 773.1 0 30 236 29 773.1 0 29 237 19 773.1 0 19 238 17 773.1 0 17 239 40 773.1 0 40 240 64 773.1 0 64 241 67 773.1 0 67 242 22 773.1 0 22 243 31 773.1 0 31 244 17 773.1 0 17 245 18 773.1 0 18 246 21 773.1 0 21 247 41 773.1 0 41 248 19 773.1 0 19 249 36 773.1 0 36 250 70 773.1 0 70 251 39 773.1 0 39 252 27 773.1 0 27 253 16 773.1 0 16 254 19 773.1 0 19 255 18 773.1 0 18 256 21 773.1 0 21 257 65 773.1 0 65 258 89 773.1 0 89 259 139 773.1 0 139 260 228 773.1 0 228 261 154 773.1 0 154 262 124 773.1 0 124 263 19 773.1 0 19 264 22 773.1 0 22 265 36 773.1 0 36 266 33 773.1 0 33 267 40 773.1 0 40 268 34 773.1 0 34 269 38 773.1 0 38 270 27 773.1 0 27 271 19 773.1 0 19 272 22 773.1 0 22 273 24 773.1 0 24 274 45 773.1 0 45 275 66 773.1 0 66 276 191 773.1 0 191 277 273 773.1 0 273 278 498 773.1 0 498 279 181 773.1 0 181 280 217 773.1 0 217 281 65 773.1 0 65 282 56 773.1 0 56 283 78 773.1 0 78 284 52 773.1 0 52 285 78 773.1 0 78 286 35 773.1 0 35 287 106 773.1 0 106 288 120 773.1 0 120 289 157 773.1 0 157 290 35 773.1 0 35 291 80 773.1 0 80 292 71 773.1 0 71 293 69 773.1 0 69 294 118 773.1 0 118 295 59 773.1 0 59 296 91 773.1 0 91 297 112 773.1 0 112 298 761 773.1 0 761 299 939 773.1 0 939 300 1384 773.1 0 1384 301 65 773.1 0 65 Finished in 9.28 s (67 us/read; 0.90 M reads/minute). === Summary === Total reads processed: 138,570 Reads with adapters: 35,508 (25.6%) Reads that were too short: 168 (0.1%) Reads written (passing filters): 35,340 (25.5%) Total basepairs processed: 37,417,236 bp Total written (filtered): 9,786,806 bp (26.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 35508 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 2 5 16 120 0.0 3 0 116 4 17 35112 0.0 3 34492 599 20 1 18 53 0.0 3 2 51 19 16 0.0 3 1 0 14 1 20 14 0.0 3 1 0 0 13 27 1 0.0 3 0 0 0 1 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 34 5 0.0 3 0 0 0 5 37 2 0.0 3 0 0 0 2 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 2 0.0 3 1 0 0 1 130 1 0.0 3 0 0 0 1 139 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 146 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 158 1 0.0 3 1 164 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 125 0.0 3 0 0 0 125 220 16 0.0 3 0 0 0 16 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 233 2 0.0 3 0 0 0 2 235 2 0.0 3 0 0 0 2 279 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.90 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 35,340 Reads with adapters: 373 (1.1%) Reads written (passing filters): 35,340 (100.0%) Total basepairs processed: 9,786,806 bp Total written (filtered): 9,785,678 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 373 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 76.4% C: 13.7% G: 3.2% T: 6.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 367 552.2 0 367 4 4 138.0 0 4 5 1 34.5 0 1 6 1 8.6 0 1