This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GACGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 149.50 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 740,358 (23.4%) Reads that were too short: 44,055 (1.4%) Reads written (passing filters): 696,303 (22.0%) Total basepairs processed: 953,096,634 bp Total written (filtered): 148,678,051 bp (15.6%) === Adapter 1 === Sequence: GACGGC; Type: regular 5'; Length: 6; Trimmed: 740358 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3527 773.1 0 3527 7 35361 773.1 0 35361 8 718 773.1 0 718 9 42441 773.1 0 42441 10 135 773.1 0 135 11 50 773.1 0 50 12 58 773.1 0 58 13 44 773.1 0 44 14 59 773.1 0 59 15 88 773.1 0 88 16 101 773.1 0 101 17 95 773.1 0 95 18 69 773.1 0 69 19 122 773.1 0 122 20 122 773.1 0 122 21 85 773.1 0 85 22 105 773.1 0 105 23 76 773.1 0 76 24 83 773.1 0 83 25 73 773.1 0 73 26 65 773.1 0 65 27 76 773.1 0 76 28 74 773.1 0 74 29 67 773.1 0 67 30 90 773.1 0 90 31 57 773.1 0 57 32 73 773.1 0 73 33 76 773.1 0 76 34 106 773.1 0 106 35 136 773.1 0 136 36 99 773.1 0 99 37 72 773.1 0 72 38 68 773.1 0 68 39 75 773.1 0 75 40 89 773.1 0 89 41 224 773.1 0 224 42 230 773.1 0 230 43 191 773.1 0 191 44 99 773.1 0 99 45 174 773.1 0 174 46 123 773.1 0 123 47 156 773.1 0 156 48 102 773.1 0 102 49 448 773.1 0 448 50 522 773.1 0 522 51 921 773.1 0 921 52 86 773.1 0 86 53 157 773.1 0 157 54 99 773.1 0 99 55 131 773.1 0 131 56 92 773.1 0 92 57 89 773.1 0 89 58 130 773.1 0 130 59 261 773.1 0 261 60 930 773.1 0 930 61 1535 773.1 0 1535 62 1209 773.1 0 1209 63 176 773.1 0 176 64 116 773.1 0 116 65 183 773.1 0 183 66 136 773.1 0 136 67 308 773.1 0 308 68 551 773.1 0 551 69 719 773.1 0 719 70 1061 773.1 0 1061 71 1964 773.1 0 1964 72 3163 773.1 0 3163 73 792 773.1 0 792 74 661 773.1 0 661 75 1326 773.1 0 1326 76 529 773.1 0 529 77 640 773.1 0 640 78 1075 773.1 0 1075 79 1079 773.1 0 1079 80 1591 773.1 0 1591 81 1565 773.1 0 1565 82 3011 773.1 0 3011 83 4073 773.1 0 4073 84 6008 773.1 0 6008 85 176286 773.1 0 176286 86 126760 773.1 0 126760 87 173341 773.1 0 173341 88 684 773.1 0 684 89 73 773.1 0 73 90 71 773.1 0 71 91 83 773.1 0 83 92 45 773.1 0 45 93 59 773.1 0 59 94 63 773.1 0 63 95 55 773.1 0 55 96 59 773.1 0 59 97 56 773.1 0 56 98 55 773.1 0 55 99 49 773.1 0 49 100 47 773.1 0 47 101 42 773.1 0 42 102 58 773.1 0 58 103 38 773.1 0 38 104 38 773.1 0 38 105 40 773.1 0 40 106 47 773.1 0 47 107 63 773.1 0 63 108 58 773.1 0 58 109 64 773.1 0 64 110 51 773.1 0 51 111 61 773.1 0 61 112 50 773.1 0 50 113 63 773.1 0 63 114 50 773.1 0 50 115 64 773.1 0 64 116 50 773.1 0 50 117 61 773.1 0 61 118 54 773.1 0 54 119 51 773.1 0 51 120 49 773.1 0 49 121 77 773.1 0 77 122 143 773.1 0 143 123 154 773.1 0 154 124 183 773.1 0 183 125 75 773.1 0 75 126 65 773.1 0 65 127 77 773.1 0 77 128 118 773.1 0 118 129 120 773.1 0 120 130 313 773.1 0 313 131 345 773.1 0 345 132 236 773.1 0 236 133 221 773.1 0 221 134 203 773.1 0 203 135 209 773.1 0 209 136 115 773.1 0 115 137 85 773.1 0 85 138 68 773.1 0 68 139 91 773.1 0 91 140 99 773.1 0 99 141 83 773.1 0 83 142 102 773.1 0 102 143 119 773.1 0 119 144 81 773.1 0 81 145 86 773.1 0 86 146 100 773.1 0 100 147 151 773.1 0 151 148 181 773.1 0 181 149 1298 773.1 0 1298 150 847 773.1 0 847 151 974 773.1 0 974 152 1641 773.1 0 1641 153 2309 773.1 0 2309 154 1974 773.1 0 1974 155 1467 773.1 0 1467 156 338 773.1 0 338 157 100 773.1 0 100 158 93 773.1 0 93 159 127 773.1 0 127 160 166 773.1 0 166 161 169 773.1 0 169 162 312 773.1 0 312 163 129 773.1 0 129 164 216 773.1 0 216 165 447 773.1 0 447 166 206 773.1 0 206 167 183 773.1 0 183 168 482 773.1 0 482 169 343 773.1 0 343 170 578 773.1 0 578 171 483 773.1 0 483 172 569 773.1 0 569 173 772 773.1 0 772 174 1253 773.1 0 1253 175 22991 773.1 0 22991 176 19888 773.1 0 19888 177 27630 773.1 0 27630 178 142 773.1 0 142 179 55 773.1 0 55 180 69 773.1 0 69 181 174 773.1 0 174 182 162 773.1 0 162 183 161 773.1 0 161 184 69 773.1 0 69 185 77 773.1 0 77 186 124 773.1 0 124 187 76 773.1 0 76 188 62 773.1 0 62 189 89 773.1 0 89 190 111 773.1 0 111 191 106 773.1 0 106 192 91 773.1 0 91 193 81 773.1 0 81 194 80 773.1 0 80 195 92 773.1 0 92 196 89 773.1 0 89 197 74 773.1 0 74 198 84 773.1 0 84 199 79 773.1 0 79 200 62 773.1 0 62 201 100 773.1 0 100 202 65 773.1 0 65 203 85 773.1 0 85 204 110 773.1 0 110 205 109 773.1 0 109 206 157 773.1 0 157 207 94 773.1 0 94 208 126 773.1 0 126 209 160 773.1 0 160 210 207 773.1 0 207 211 251 773.1 0 251 212 352 773.1 0 352 213 8400 773.1 0 8400 214 3597 773.1 0 3597 215 6749 773.1 0 6749 216 130 773.1 0 130 217 205 773.1 0 205 218 320 773.1 0 320 219 232 773.1 0 232 220 173 773.1 0 173 221 139 773.1 0 139 222 104 773.1 0 104 223 61 773.1 0 61 224 79 773.1 0 79 225 80 773.1 0 80 226 90 773.1 0 90 227 162 773.1 0 162 228 379 773.1 0 379 229 296 773.1 0 296 230 403 773.1 0 403 231 1345 773.1 0 1345 232 537 773.1 0 537 233 902 773.1 0 902 234 140 773.1 0 140 235 148 773.1 0 148 236 125 773.1 0 125 237 92 773.1 0 92 238 114 773.1 0 114 239 82 773.1 0 82 240 101 773.1 0 101 241 100 773.1 0 100 242 86 773.1 0 86 243 97 773.1 0 97 244 82 773.1 0 82 245 87 773.1 0 87 246 110 773.1 0 110 247 156 773.1 0 156 248 201 773.1 0 201 249 242 773.1 0 242 250 217 773.1 0 217 251 196 773.1 0 196 252 147 773.1 0 147 253 585 773.1 0 585 254 853 773.1 0 853 255 743 773.1 0 743 256 497 773.1 0 497 257 398 773.1 0 398 258 339 773.1 0 339 259 204 773.1 0 204 260 145 773.1 0 145 261 130 773.1 0 130 262 168 773.1 0 168 263 74 773.1 0 74 264 83 773.1 0 83 265 69 773.1 0 69 266 114 773.1 0 114 267 116 773.1 0 116 268 116 773.1 0 116 269 109 773.1 0 109 270 126 773.1 0 126 271 132 773.1 0 132 272 106 773.1 0 106 273 68 773.1 0 68 274 109 773.1 0 109 275 127 773.1 0 127 276 245 773.1 0 245 277 533 773.1 0 533 278 706 773.1 0 706 279 838 773.1 0 838 280 160 773.1 0 160 281 148 773.1 0 148 282 152 773.1 0 152 283 123 773.1 0 123 284 103 773.1 0 103 285 501 773.1 0 501 286 543 773.1 0 543 287 475 773.1 0 475 288 231 773.1 0 231 289 188 773.1 0 188 290 223 773.1 0 223 291 158 773.1 0 158 292 176 773.1 0 176 293 362 773.1 0 362 294 334 773.1 0 334 295 335 773.1 0 335 296 330 773.1 0 330 297 729 773.1 0 729 298 830 773.1 0 830 299 730 773.1 0 730 300 476 773.1 0 476 301 393 773.1 0 393 Finished in 42.25 s (61 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 696,303 Reads with adapters: 35,341 (5.1%) Reads that were too short: 395 (0.1%) Reads written (passing filters): 34,946 (5.0%) Total basepairs processed: 148,678,051 bp Total written (filtered): 9,542,109 bp (6.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 35341 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 5 0.0 3 0 0 2 3 19 179 0.0 3 0 141 22 9 7 20 34310 0.0 4 32825 1243 182 43 17 21 68 0.0 4 4 60 2 0 2 22 7 0.0 4 5 0 2 23 53 0.0 4 0 2 0 40 11 24 1 0.0 4 0 0 0 1 27 1 0.0 4 0 0 0 0 1 31 2 0.0 4 1 0 0 0 1 40 2 0.0 4 0 0 0 0 2 43 1 0.0 4 1 48 1 0.0 4 0 0 0 0 1 49 168 0.0 4 0 0 0 0 168 52 2 0.0 4 0 0 0 0 2 57 11 0.0 4 0 0 0 0 11 58 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 66 2 0.0 4 0 0 0 0 2 67 4 0.0 4 0 0 0 1 3 68 3 0.0 4 0 0 0 0 3 73 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 79 3 0.0 4 1 0 0 1 1 82 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 88 3 0.0 4 0 0 0 0 3 89 3 0.0 4 0 0 0 1 2 90 5 0.0 4 0 0 0 0 5 91 18 0.0 4 0 0 0 0 18 92 2 0.0 4 0 0 0 0 2 100 3 0.0 4 0 0 0 0 3 105 1 0.0 4 0 0 0 0 1 109 2 0.0 4 0 0 0 1 1 110 3 0.0 4 0 1 0 0 2 111 2 0.0 4 0 0 0 0 2 115 3 0.0 4 0 0 0 0 3 118 1 0.0 4 0 1 123 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 97 0.0 4 0 0 0 0 97 128 1 0.0 4 0 1 129 12 0.0 4 0 0 0 0 12 132 2 0.0 4 0 0 0 0 2 133 1 0.0 4 0 0 0 0 1 134 1 0.0 4 0 0 0 0 1 135 6 0.0 4 0 0 0 0 6 136 12 0.0 4 0 0 0 0 12 137 5 0.0 4 0 0 0 1 4 138 3 0.0 4 0 0 0 0 3 139 1 0.0 4 0 0 0 0 1 142 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 78 0.0 4 0 0 0 3 75 151 49 0.0 4 0 0 1 0 48 152 7 0.0 4 0 0 0 0 7 153 2 0.0 4 0 0 0 0 2 156 2 0.0 4 0 0 0 0 2 157 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 159 2 0.0 4 0 0 0 0 2 160 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 174 1 0.0 4 0 0 0 0 1 179 3 0.0 4 0 0 0 0 3 180 1 0.0 4 0 0 0 0 1 181 2 0.0 4 0 0 0 0 2 182 11 0.0 4 0 0 0 0 11 183 2 0.0 4 0 0 0 0 2 184 25 0.0 4 0 0 0 0 25 185 27 0.0 4 0 0 0 2 25 186 13 0.0 4 0 0 0 0 13 187 7 0.0 4 0 0 0 0 7 188 2 0.0 4 0 0 0 0 2 189 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 198 2 0.0 4 0 0 0 0 2 199 3 0.0 4 0 0 0 1 2 200 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 205 1 0.0 4 0 0 0 0 1 207 3 0.0 4 0 0 0 0 3 208 3 0.0 4 0 0 0 0 3 213 2 0.0 4 0 0 0 0 2 214 2 0.0 4 0 0 0 0 2 215 10 0.0 4 0 0 0 1 9 216 7 0.0 4 0 0 0 0 7 218 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 222 1 0.0 4 0 0 0 0 1 224 6 0.0 4 0 0 0 0 6 225 4 0.0 4 0 0 0 0 4 226 1 0.0 4 0 0 0 0 1 228 7 0.0 4 0 0 0 0 7 229 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 263 1 0.0 4 0 0 0 0 1 264 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 272 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.93 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 34,946 Reads with adapters: 2,817 (8.1%) Reads written (passing filters): 34,946 (100.0%) Total basepairs processed: 9,542,109 bp Total written (filtered): 9,503,773 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2817 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.2% C: 8.0% G: 85.6% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 117 546.0 0 117 4 13 136.5 0 13 5 1 34.1 0 1 9 10 0.1 0 0 10 10 33 0.0 1 0 33 11 2338 0.0 1 52 2286 12 199 0.0 1 4 195 13 44 0.0 1 5 39 14 4 0.0 1 2 2 15 5 0.0 1 0 5 19 3 0.0 1 2 1 152 34 0.0 1 25 9 153 1 0.0 1 1 210 4 0.0 1 3 1 225 9 0.0 1 9 226 1 0.0 1 1 230 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.