This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 146.15 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 157,358 (5.0%) Reads that were too short: 7,779 (0.2%) Reads written (passing filters): 149,579 (4.7%) Total basepairs processed: 953,096,634 bp Total written (filtered): 42,677,008 bp (4.5%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 157358 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4340 773.1 0 4340 7 4928 773.1 0 4928 8 75520 773.1 0 75520 9 54082 773.1 0 54082 10 251 773.1 0 251 11 9 773.1 0 9 12 3 773.1 0 3 13 4 773.1 0 4 14 1 773.1 0 1 16 3 773.1 0 3 17 2 773.1 0 2 18 3 773.1 0 3 19 6 773.1 0 6 20 3 773.1 0 3 21 3 773.1 0 3 22 8 773.1 0 8 23 22 773.1 0 22 24 6 773.1 0 6 25 10 773.1 0 10 26 2 773.1 0 2 27 2 773.1 0 2 28 2 773.1 0 2 29 17 773.1 0 17 30 18 773.1 0 18 31 8 773.1 0 8 32 6 773.1 0 6 33 95 773.1 0 95 34 36 773.1 0 36 35 15 773.1 0 15 36 43 773.1 0 43 37 10 773.1 0 10 38 11 773.1 0 11 39 32 773.1 0 32 40 50 773.1 0 50 41 3 773.1 0 3 42 9 773.1 0 9 43 4 773.1 0 4 44 7 773.1 0 7 45 10 773.1 0 10 46 10 773.1 0 10 47 3 773.1 0 3 48 10 773.1 0 10 49 5 773.1 0 5 50 6 773.1 0 6 51 7 773.1 0 7 52 8 773.1 0 8 53 11 773.1 0 11 54 13 773.1 0 13 55 6 773.1 0 6 56 4 773.1 0 4 57 6 773.1 0 6 58 71 773.1 0 71 59 182 773.1 0 182 60 109 773.1 0 109 61 4 773.1 0 4 62 5 773.1 0 5 63 7 773.1 0 7 64 7 773.1 0 7 65 3 773.1 0 3 66 5 773.1 0 5 67 6 773.1 0 6 68 3 773.1 0 3 69 9 773.1 0 9 70 22 773.1 0 22 71 45 773.1 0 45 72 13 773.1 0 13 73 26 773.1 0 26 74 15 773.1 0 15 75 13 773.1 0 13 76 103 773.1 0 103 77 238 773.1 0 238 78 129 773.1 0 129 79 16 773.1 0 16 80 172 773.1 0 172 81 162 773.1 0 162 82 135 773.1 0 135 83 10 773.1 0 10 84 6 773.1 0 6 85 25 773.1 0 25 86 7 773.1 0 7 87 16 773.1 0 16 88 23 773.1 0 23 89 15 773.1 0 15 90 15 773.1 0 15 91 12 773.1 0 12 92 5 773.1 0 5 93 17 773.1 0 17 94 18 773.1 0 18 95 17 773.1 0 17 96 9 773.1 0 9 97 16 773.1 0 16 98 15 773.1 0 15 99 33 773.1 0 33 100 591 773.1 0 591 101 992 773.1 0 992 102 843 773.1 0 843 103 12 773.1 0 12 104 13 773.1 0 13 105 15 773.1 0 15 106 16 773.1 0 16 107 9 773.1 0 9 108 28 773.1 0 28 109 110 773.1 0 110 110 1360 773.1 0 1360 111 867 773.1 0 867 112 550 773.1 0 550 113 11 773.1 0 11 114 4 773.1 0 4 115 11 773.1 0 11 116 26 773.1 0 26 117 3 773.1 0 3 118 6 773.1 0 6 119 3 773.1 0 3 120 4 773.1 0 4 121 12 773.1 0 12 122 6 773.1 0 6 123 8 773.1 0 8 124 8 773.1 0 8 125 12 773.1 0 12 126 13 773.1 0 13 127 34 773.1 0 34 128 55 773.1 0 55 129 39 773.1 0 39 130 88 773.1 0 88 131 17 773.1 0 17 132 33 773.1 0 33 133 20 773.1 0 20 134 9 773.1 0 9 135 13 773.1 0 13 136 12 773.1 0 12 137 7 773.1 0 7 138 16 773.1 0 16 139 2 773.1 0 2 140 13 773.1 0 13 141 10 773.1 0 10 142 23 773.1 0 23 143 11 773.1 0 11 144 14 773.1 0 14 145 45 773.1 0 45 146 11 773.1 0 11 147 86 773.1 0 86 148 55 773.1 0 55 149 46 773.1 0 46 150 53 773.1 0 53 151 11 773.1 0 11 152 28 773.1 0 28 153 22 773.1 0 22 154 20 773.1 0 20 155 7 773.1 0 7 156 20 773.1 0 20 157 40 773.1 0 40 158 32 773.1 0 32 159 20 773.1 0 20 160 18 773.1 0 18 161 21 773.1 0 21 162 17 773.1 0 17 163 11 773.1 0 11 164 13 773.1 0 13 165 5 773.1 0 5 166 12 773.1 0 12 167 15 773.1 0 15 168 65 773.1 0 65 169 97 773.1 0 97 170 89 773.1 0 89 171 6 773.1 0 6 172 9 773.1 0 9 173 9 773.1 0 9 174 6 773.1 0 6 175 8 773.1 0 8 176 9 773.1 0 9 177 31 773.1 0 31 178 14 773.1 0 14 179 15 773.1 0 15 180 17 773.1 0 17 181 17 773.1 0 17 182 37 773.1 0 37 183 47 773.1 0 47 184 79 773.1 0 79 185 32 773.1 0 32 186 17 773.1 0 17 187 17 773.1 0 17 188 18 773.1 0 18 189 40 773.1 0 40 190 11 773.1 0 11 191 12 773.1 0 12 192 14 773.1 0 14 193 15 773.1 0 15 194 26 773.1 0 26 195 28 773.1 0 28 196 43 773.1 0 43 197 107 773.1 0 107 198 99 773.1 0 99 199 244 773.1 0 244 200 177 773.1 0 177 201 135 773.1 0 135 202 362 773.1 0 362 203 254 773.1 0 254 204 618 773.1 0 618 205 63 773.1 0 63 206 41 773.1 0 41 207 18 773.1 0 18 208 28 773.1 0 28 209 30 773.1 0 30 210 30 773.1 0 30 211 31 773.1 0 31 212 22 773.1 0 22 213 22 773.1 0 22 214 39 773.1 0 39 215 24 773.1 0 24 216 19 773.1 0 19 217 103 773.1 0 103 218 121 773.1 0 121 219 202 773.1 0 202 220 52 773.1 0 52 221 32 773.1 0 32 222 354 773.1 0 354 223 485 773.1 0 485 224 431 773.1 0 431 225 82 773.1 0 82 226 25 773.1 0 25 227 36 773.1 0 36 228 32 773.1 0 32 229 57 773.1 0 57 230 24 773.1 0 24 231 15 773.1 0 15 232 13 773.1 0 13 233 19 773.1 0 19 234 33 773.1 0 33 235 25 773.1 0 25 236 27 773.1 0 27 237 129 773.1 0 129 238 28 773.1 0 28 239 39 773.1 0 39 240 23 773.1 0 23 241 60 773.1 0 60 242 46 773.1 0 46 243 72 773.1 0 72 244 34 773.1 0 34 245 26 773.1 0 26 246 16 773.1 0 16 247 24 773.1 0 24 248 23 773.1 0 23 249 32 773.1 0 32 250 39 773.1 0 39 251 39 773.1 0 39 252 47 773.1 0 47 253 34 773.1 0 34 254 53 773.1 0 53 255 83 773.1 0 83 256 159 773.1 0 159 257 167 773.1 0 167 258 251 773.1 0 251 259 93 773.1 0 93 260 82 773.1 0 82 261 37 773.1 0 37 262 56 773.1 0 56 263 46 773.1 0 46 264 48 773.1 0 48 265 35 773.1 0 35 266 24 773.1 0 24 267 31 773.1 0 31 268 29 773.1 0 29 269 28 773.1 0 28 270 39 773.1 0 39 271 58 773.1 0 58 272 47 773.1 0 47 273 50 773.1 0 50 274 26 773.1 0 26 275 41 773.1 0 41 276 55 773.1 0 55 277 66 773.1 0 66 278 93 773.1 0 93 279 56 773.1 0 56 280 61 773.1 0 61 281 34 773.1 0 34 282 38 773.1 0 38 283 53 773.1 0 53 284 37 773.1 0 37 285 59 773.1 0 59 286 36 773.1 0 36 287 51 773.1 0 51 288 47 773.1 0 47 289 87 773.1 0 87 290 109 773.1 0 109 291 139 773.1 0 139 292 106 773.1 0 106 293 62 773.1 0 62 294 47 773.1 0 47 295 82 773.1 0 82 296 101 773.1 0 101 297 54 773.1 0 54 298 96 773.1 0 96 299 80 773.1 0 80 300 104 773.1 0 104 301 63 773.1 0 63 Finished in 11.57 s (77 us/read; 0.78 M reads/minute). === Summary === Total reads processed: 149,579 Reads with adapters: 59,477 (39.8%) Reads that were too short: 41 (0.0%) Reads written (passing filters): 59,436 (39.7%) Total basepairs processed: 42,677,008 bp Total written (filtered): 16,131,582 bp (37.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 59477 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 2 0.0 3 0 0 0 1 1 18 22 0.0 3 0 0 9 8 5 19 243 0.0 3 0 157 38 32 16 20 54788 0.0 4 52051 2223 369 115 30 21 3737 0.0 4 3462 223 33 11 8 22 21 0.0 4 2 5 10 2 2 23 215 0.0 4 1 3 0 151 60 24 15 0.0 4 2 0 0 5 8 25 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 28 1 0.0 4 1 32 1 0.0 4 0 0 1 35 1 0.0 4 1 39 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 69 2 0.0 4 0 0 0 0 2 76 3 0.0 4 0 0 0 0 3 89 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 59 0.0 4 0 0 0 0 59 128 309 0.0 4 0 0 0 0 309 129 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 153 2 0.0 4 0 0 0 0 2 156 1 0.0 4 0 0 0 0 1 157 1 0.0 4 1 177 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 215 1 0.0 4 0 0 0 1 216 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 4 0.0 4 0 0 0 0 4 230 4 0.0 4 0 0 0 0 4 231 3 0.0 4 0 0 0 0 3 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 1 237 2 0.0 4 0 0 0 0 2 242 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 277 1 0.0 4 0 0 0 0 1 278 1 0.0 4 0 0 0 0 1 279 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.54 s (60 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 59,436 Reads with adapters: 16,213 (27.3%) Reads written (passing filters): 59,436 (100.0%) Total basepairs processed: 16,131,582 bp Total written (filtered): 15,969,185 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 16213 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 5.1% G: 92.1% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 30 928.7 0 30 4 17 232.2 0 17 5 1 58.0 0 1 6 13 14.5 0 13 8 7 0.9 0 7 9 7396 0.2 0 394 7002 10 6435 0.1 1 380 6055 11 2216 0.0 1 58 2158 12 42 0.0 1 1 41 13 22 0.0 1 0 22 14 1 0.0 1 0 1 42 1 0.0 1 1 72 1 0.0 1 0 1 148 1 0.0 1 0 1 150 11 0.0 1 7 4 208 9 0.0 1 5 4 209 1 0.0 1 1 223 5 0.0 1 5 224 3 0.0 1 3 226 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.