This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 143.92 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 153,527 (4.8%) Reads that were too short: 2,465 (0.1%) Reads written (passing filters): 151,062 (4.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 43,526,838 bp (4.6%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 153527 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3846 773.1 0 3846 7 5207 773.1 0 5207 8 55411 773.1 0 55411 9 79925 773.1 0 79925 10 143 773.1 0 143 11 6 773.1 0 6 12 4 773.1 0 4 13 2 773.1 0 2 14 1 773.1 0 1 15 1 773.1 0 1 16 1 773.1 0 1 17 1 773.1 0 1 18 3 773.1 0 3 19 3 773.1 0 3 20 3 773.1 0 3 22 1 773.1 0 1 23 4 773.1 0 4 24 1 773.1 0 1 25 2 773.1 0 2 26 2 773.1 0 2 27 2 773.1 0 2 29 7 773.1 0 7 30 14 773.1 0 14 31 4 773.1 0 4 32 3 773.1 0 3 33 7 773.1 0 7 34 9 773.1 0 9 35 8 773.1 0 8 36 35 773.1 0 35 37 2 773.1 0 2 38 18 773.1 0 18 39 36 773.1 0 36 40 13 773.1 0 13 41 5 773.1 0 5 42 3 773.1 0 3 43 8 773.1 0 8 44 2 773.1 0 2 45 3 773.1 0 3 46 17 773.1 0 17 47 4 773.1 0 4 48 3 773.1 0 3 49 2 773.1 0 2 50 3 773.1 0 3 51 2 773.1 0 2 52 2 773.1 0 2 53 2 773.1 0 2 54 9 773.1 0 9 55 6 773.1 0 6 56 2 773.1 0 2 57 4 773.1 0 4 58 25 773.1 0 25 59 129 773.1 0 129 60 50 773.1 0 50 61 1 773.1 0 1 62 3 773.1 0 3 63 6 773.1 0 6 64 3 773.1 0 3 65 6 773.1 0 6 66 6 773.1 0 6 67 5 773.1 0 5 68 6 773.1 0 6 69 9 773.1 0 9 70 12 773.1 0 12 71 34 773.1 0 34 72 8 773.1 0 8 73 13 773.1 0 13 74 3 773.1 0 3 75 4 773.1 0 4 76 79 773.1 0 79 77 204 773.1 0 204 78 81 773.1 0 81 79 2 773.1 0 2 80 86 773.1 0 86 81 75 773.1 0 75 82 24 773.1 0 24 83 8 773.1 0 8 84 1 773.1 0 1 85 3 773.1 0 3 86 5 773.1 0 5 87 3 773.1 0 3 88 2 773.1 0 2 89 7 773.1 0 7 90 4 773.1 0 4 91 4 773.1 0 4 92 5 773.1 0 5 93 4 773.1 0 4 94 13 773.1 0 13 95 13 773.1 0 13 96 6 773.1 0 6 97 12 773.1 0 12 98 5 773.1 0 5 99 21 773.1 0 21 100 277 773.1 0 277 101 476 773.1 0 476 102 456 773.1 0 456 103 8 773.1 0 8 104 9 773.1 0 9 105 13 773.1 0 13 106 16 773.1 0 16 107 18 773.1 0 18 108 19 773.1 0 19 109 117 773.1 0 117 110 1208 773.1 0 1208 111 841 773.1 0 841 112 512 773.1 0 512 113 8 773.1 0 8 114 3 773.1 0 3 115 7 773.1 0 7 116 20 773.1 0 20 117 7 773.1 0 7 118 2 773.1 0 2 119 2 773.1 0 2 120 3 773.1 0 3 121 2 773.1 0 2 122 3 773.1 0 3 123 2 773.1 0 2 124 7 773.1 0 7 125 6 773.1 0 6 126 16 773.1 0 16 127 29 773.1 0 29 128 64 773.1 0 64 129 46 773.1 0 46 130 49 773.1 0 49 131 3 773.1 0 3 132 4 773.1 0 4 133 7 773.1 0 7 134 11 773.1 0 11 135 14 773.1 0 14 136 3 773.1 0 3 137 13 773.1 0 13 138 9 773.1 0 9 139 3 773.1 0 3 140 6 773.1 0 6 141 6 773.1 0 6 142 5 773.1 0 5 143 8 773.1 0 8 144 7 773.1 0 7 145 38 773.1 0 38 146 5 773.1 0 5 147 60 773.1 0 60 148 21 773.1 0 21 149 19 773.1 0 19 150 13 773.1 0 13 151 6 773.1 0 6 152 17 773.1 0 17 153 32 773.1 0 32 154 13 773.1 0 13 155 6 773.1 0 6 156 4 773.1 0 4 157 8 773.1 0 8 158 3 773.1 0 3 159 8 773.1 0 8 160 7 773.1 0 7 161 6 773.1 0 6 162 7 773.1 0 7 163 6 773.1 0 6 164 2 773.1 0 2 165 3 773.1 0 3 166 6 773.1 0 6 167 12 773.1 0 12 168 76 773.1 0 76 169 106 773.1 0 106 170 85 773.1 0 85 171 4 773.1 0 4 172 4 773.1 0 4 173 2 773.1 0 2 174 2 773.1 0 2 175 2 773.1 0 2 176 7 773.1 0 7 177 7 773.1 0 7 178 4 773.1 0 4 179 7 773.1 0 7 180 7 773.1 0 7 181 6 773.1 0 6 182 6 773.1 0 6 183 12 773.1 0 12 184 20 773.1 0 20 185 14 773.1 0 14 186 6 773.1 0 6 187 5 773.1 0 5 188 10 773.1 0 10 189 4 773.1 0 4 190 11 773.1 0 11 191 10 773.1 0 10 192 3 773.1 0 3 193 10 773.1 0 10 194 10 773.1 0 10 195 7 773.1 0 7 196 15 773.1 0 15 197 28 773.1 0 28 198 21 773.1 0 21 199 51 773.1 0 51 200 38 773.1 0 38 201 23 773.1 0 23 202 70 773.1 0 70 203 38 773.1 0 38 204 90 773.1 0 90 205 16 773.1 0 16 206 11 773.1 0 11 207 5 773.1 0 5 208 10 773.1 0 10 209 10 773.1 0 10 210 5 773.1 0 5 211 9 773.1 0 9 212 7 773.1 0 7 213 6 773.1 0 6 214 11 773.1 0 11 215 10 773.1 0 10 216 10 773.1 0 10 217 34 773.1 0 34 218 31 773.1 0 31 219 75 773.1 0 75 220 23 773.1 0 23 221 6 773.1 0 6 222 85 773.1 0 85 223 113 773.1 0 113 224 92 773.1 0 92 225 21 773.1 0 21 226 10 773.1 0 10 227 15 773.1 0 15 228 22 773.1 0 22 229 22 773.1 0 22 230 6 773.1 0 6 231 6 773.1 0 6 232 9 773.1 0 9 233 7 773.1 0 7 234 7 773.1 0 7 235 16 773.1 0 16 236 11 773.1 0 11 237 83 773.1 0 83 238 16 773.1 0 16 239 19 773.1 0 19 240 18 773.1 0 18 241 20 773.1 0 20 242 24 773.1 0 24 243 24 773.1 0 24 244 14 773.1 0 14 245 4 773.1 0 4 246 5 773.1 0 5 247 8 773.1 0 8 248 13 773.1 0 13 249 15 773.1 0 15 250 11 773.1 0 11 251 17 773.1 0 17 252 26 773.1 0 26 253 11 773.1 0 11 254 17 773.1 0 17 255 28 773.1 0 28 256 66 773.1 0 66 257 61 773.1 0 61 258 88 773.1 0 88 259 31 773.1 0 31 260 28 773.1 0 28 261 14 773.1 0 14 262 31 773.1 0 31 263 17 773.1 0 17 264 25 773.1 0 25 265 8 773.1 0 8 266 17 773.1 0 17 267 13 773.1 0 13 268 12 773.1 0 12 269 20 773.1 0 20 270 21 773.1 0 21 271 16 773.1 0 16 272 12 773.1 0 12 273 9 773.1 0 9 274 15 773.1 0 15 275 16 773.1 0 16 276 21 773.1 0 21 277 24 773.1 0 24 278 25 773.1 0 25 279 14 773.1 0 14 280 17 773.1 0 17 281 9 773.1 0 9 282 14 773.1 0 14 283 15 773.1 0 15 284 15 773.1 0 15 285 18 773.1 0 18 286 20 773.1 0 20 287 21 773.1 0 21 288 20 773.1 0 20 289 29 773.1 0 29 290 44 773.1 0 44 291 42 773.1 0 42 292 31 773.1 0 31 293 22 773.1 0 22 294 19 773.1 0 19 295 27 773.1 0 27 296 31 773.1 0 31 297 30 773.1 0 30 298 28 773.1 0 28 299 35 773.1 0 35 300 57 773.1 0 57 301 25 773.1 0 25 Finished in 10.84 s (72 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 151,062 Reads with adapters: 58,143 (38.5%) Reads that were too short: 133 (0.1%) Reads written (passing filters): 58,010 (38.4%) Total basepairs processed: 43,526,838 bp Total written (filtered): 16,010,131 bp (36.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 58143 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 3 1 16 217 0.0 3 0 213 4 17 54538 0.0 3 53579 927 28 4 18 3185 0.0 3 3017 163 5 19 18 0.0 3 3 3 11 1 20 15 0.0 3 1 0 1 13 21 2 0.0 3 0 0 0 2 32 1 0.0 3 1 52 1 0.0 3 0 0 0 1 75 1 0.0 3 1 82 19 0.0 3 0 0 0 19 85 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 195 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 108 0.0 3 0 0 1 107 218 2 0.0 3 0 0 1 1 222 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 5 0.0 3 0 1 0 4 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.23 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 58,010 Reads with adapters: 451 (0.8%) Reads written (passing filters): 58,010 (100.0%) Total basepairs processed: 16,010,131 bp Total written (filtered): 16,008,461 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 451 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 33.7% C: 2.2% G: 61.4% T: 2.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 138 906.4 0 138 4 309 226.6 0 309 5 4 56.7 0 4