This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 148.30 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 337,106 (10.6%) Reads that were too short: 5,133 (0.2%) Reads written (passing filters): 331,973 (10.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 57,543,588 bp (6.0%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 337106 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 379 773.1 0 379 7 20064 773.1 0 20064 8 17809 773.1 0 17809 9 24 773.1 0 24 10 16 773.1 0 16 11 161 773.1 0 161 12 127 773.1 0 127 13 280 773.1 0 280 14 15 773.1 0 15 15 14 773.1 0 14 16 7 773.1 0 7 17 8 773.1 0 8 18 15 773.1 0 15 19 18 773.1 0 18 20 26 773.1 0 26 21 16 773.1 0 16 22 19 773.1 0 19 23 6 773.1 0 6 24 7 773.1 0 7 25 37 773.1 0 37 26 36 773.1 0 36 27 43 773.1 0 43 28 9 773.1 0 9 29 4 773.1 0 4 30 7 773.1 0 7 31 32 773.1 0 32 32 20 773.1 0 20 33 23 773.1 0 23 34 27 773.1 0 27 35 19 773.1 0 19 36 15 773.1 0 15 37 20 773.1 0 20 38 48 773.1 0 48 39 19 773.1 0 19 40 51 773.1 0 51 41 7 773.1 0 7 42 11 773.1 0 11 43 43 773.1 0 43 44 21 773.1 0 21 45 16 773.1 0 16 46 26 773.1 0 26 47 8 773.1 0 8 48 18 773.1 0 18 49 7 773.1 0 7 50 8 773.1 0 8 51 5 773.1 0 5 52 12 773.1 0 12 53 17 773.1 0 17 54 19 773.1 0 19 55 20 773.1 0 20 56 10 773.1 0 10 57 16 773.1 0 16 58 20 773.1 0 20 59 19 773.1 0 19 60 15 773.1 0 15 61 16 773.1 0 16 62 11 773.1 0 11 63 9 773.1 0 9 64 9 773.1 0 9 65 8 773.1 0 8 66 5 773.1 0 5 67 7 773.1 0 7 68 7 773.1 0 7 69 8 773.1 0 8 70 21 773.1 0 21 71 24 773.1 0 24 72 35 773.1 0 35 73 52 773.1 0 52 74 35 773.1 0 35 75 76 773.1 0 76 76 28 773.1 0 28 77 14 773.1 0 14 78 12 773.1 0 12 79 13 773.1 0 13 80 18 773.1 0 18 81 11 773.1 0 11 82 16 773.1 0 16 83 10 773.1 0 10 84 16 773.1 0 16 85 21 773.1 0 21 86 20 773.1 0 20 87 6 773.1 0 6 88 12 773.1 0 12 89 34 773.1 0 34 90 15 773.1 0 15 91 27 773.1 0 27 92 15 773.1 0 15 93 15 773.1 0 15 94 18 773.1 0 18 95 20 773.1 0 20 96 21 773.1 0 21 97 53 773.1 0 53 98 56 773.1 0 56 99 39 773.1 0 39 100 31 773.1 0 31 101 19 773.1 0 19 102 85 773.1 0 85 103 62 773.1 0 62 104 86 773.1 0 86 105 103 773.1 0 103 106 79 773.1 0 79 107 88 773.1 0 88 108 64 773.1 0 64 109 47 773.1 0 47 110 35 773.1 0 35 111 55 773.1 0 55 112 72 773.1 0 72 113 45 773.1 0 45 114 87 773.1 0 87 115 259 773.1 0 259 116 30 773.1 0 30 117 23 773.1 0 23 118 29 773.1 0 29 119 28 773.1 0 28 120 46 773.1 0 46 121 96 773.1 0 96 122 476 773.1 0 476 123 360 773.1 0 360 124 426 773.1 0 426 125 379 773.1 0 379 126 95 773.1 0 95 127 221 773.1 0 221 128 150 773.1 0 150 129 196 773.1 0 196 130 605 773.1 0 605 131 176 773.1 0 176 132 136 773.1 0 136 133 74 773.1 0 74 134 112 773.1 0 112 135 299 773.1 0 299 136 331 773.1 0 331 137 594 773.1 0 594 138 882 773.1 0 882 139 1210 773.1 0 1210 140 2758 773.1 0 2758 141 6652 773.1 0 6652 142 106795 773.1 0 106795 143 61634 773.1 0 61634 144 81361 773.1 0 81361 145 387 773.1 0 387 146 91 773.1 0 91 147 72 773.1 0 72 148 37 773.1 0 37 149 58 773.1 0 58 150 68 773.1 0 68 151 47 773.1 0 47 152 25 773.1 0 25 153 24 773.1 0 24 154 23 773.1 0 23 155 41 773.1 0 41 156 61 773.1 0 61 157 97 773.1 0 97 158 88 773.1 0 88 159 151 773.1 0 151 160 281 773.1 0 281 161 627 773.1 0 627 162 8986 773.1 0 8986 163 5391 773.1 0 5391 164 6305 773.1 0 6305 165 36 773.1 0 36 166 21 773.1 0 21 167 42 773.1 0 42 168 26 773.1 0 26 169 41 773.1 0 41 170 24 773.1 0 24 171 13 773.1 0 13 172 14 773.1 0 14 173 4 773.1 0 4 174 4 773.1 0 4 175 6 773.1 0 6 176 22 773.1 0 22 177 58 773.1 0 58 178 23 773.1 0 23 179 18 773.1 0 18 180 21 773.1 0 21 181 9 773.1 0 9 182 22 773.1 0 22 183 31 773.1 0 31 184 22 773.1 0 22 185 17 773.1 0 17 186 20 773.1 0 20 187 35 773.1 0 35 188 171 773.1 0 171 189 88 773.1 0 88 190 178 773.1 0 178 191 19 773.1 0 19 192 9 773.1 0 9 193 9 773.1 0 9 194 19 773.1 0 19 195 16 773.1 0 16 196 25 773.1 0 25 197 15 773.1 0 15 198 18 773.1 0 18 199 21 773.1 0 21 200 16 773.1 0 16 201 17 773.1 0 17 202 31 773.1 0 31 203 22 773.1 0 22 204 20 773.1 0 20 205 19 773.1 0 19 206 20 773.1 0 20 207 21 773.1 0 21 208 30 773.1 0 30 209 49 773.1 0 49 210 54 773.1 0 54 211 51 773.1 0 51 212 53 773.1 0 53 213 64 773.1 0 64 214 187 773.1 0 187 215 307 773.1 0 307 216 251 773.1 0 251 217 97 773.1 0 97 218 31 773.1 0 31 219 18 773.1 0 18 220 16 773.1 0 16 221 12 773.1 0 12 222 31 773.1 0 31 223 55 773.1 0 55 224 42 773.1 0 42 225 45 773.1 0 45 226 38 773.1 0 38 227 50 773.1 0 50 228 21 773.1 0 21 229 15 773.1 0 15 230 17 773.1 0 17 231 13 773.1 0 13 232 45 773.1 0 45 233 58 773.1 0 58 234 58 773.1 0 58 235 22 773.1 0 22 236 21 773.1 0 21 237 25 773.1 0 25 238 39 773.1 0 39 239 49 773.1 0 49 240 48 773.1 0 48 241 26 773.1 0 26 242 21 773.1 0 21 243 39 773.1 0 39 244 29 773.1 0 29 245 38 773.1 0 38 246 39 773.1 0 39 247 23 773.1 0 23 248 20 773.1 0 20 249 23 773.1 0 23 250 20 773.1 0 20 251 28 773.1 0 28 252 18 773.1 0 18 253 15 773.1 0 15 254 23 773.1 0 23 255 63 773.1 0 63 256 69 773.1 0 69 257 56 773.1 0 56 258 57 773.1 0 57 259 37 773.1 0 37 260 62 773.1 0 62 261 182 773.1 0 182 262 163 773.1 0 163 263 218 773.1 0 218 264 66 773.1 0 66 265 30 773.1 0 30 266 22 773.1 0 22 267 19 773.1 0 19 268 26 773.1 0 26 269 57 773.1 0 57 270 54 773.1 0 54 271 40 773.1 0 40 272 20 773.1 0 20 273 30 773.1 0 30 274 36 773.1 0 36 275 49 773.1 0 49 276 73 773.1 0 73 277 50 773.1 0 50 278 47 773.1 0 47 279 116 773.1 0 116 280 117 773.1 0 117 281 153 773.1 0 153 282 64 773.1 0 64 283 56 773.1 0 56 284 54 773.1 0 54 285 62 773.1 0 62 286 30 773.1 0 30 287 40 773.1 0 40 288 18 773.1 0 18 289 27 773.1 0 27 290 49 773.1 0 49 291 23 773.1 0 23 292 39 773.1 0 39 293 35 773.1 0 35 294 31 773.1 0 31 295 42 773.1 0 42 296 67 773.1 0 67 297 60 773.1 0 60 298 45 773.1 0 45 299 24 773.1 0 24 300 33 773.1 0 33 301 15 773.1 0 15 Finished in 18.85 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 331,973 Reads with adapters: 18,526 (5.6%) Reads that were too short: 32 (0.0%) Reads written (passing filters): 18,494 (5.6%) Total basepairs processed: 57,543,588 bp Total written (filtered): 5,019,294 bp (8.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18526 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 8 0.0 3 0 0 3 0 5 19 98 0.0 3 0 74 12 7 5 20 18023 0.0 4 17192 677 123 19 12 21 43 0.0 4 10 31 2 22 12 0.0 4 2 3 4 3 23 40 0.0 4 2 1 1 25 11 24 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 37 16 0.0 4 0 0 0 0 16 38 8 0.0 4 0 0 0 0 8 43 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 61 3 0.0 4 0 0 0 0 3 69 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 81 3 0.0 4 0 0 0 0 3 84 1 0.0 4 0 0 0 1 88 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 95 1 0.0 4 1 96 1 0.0 4 0 0 0 0 1 123 7 0.0 4 0 0 0 0 7 126 7 0.0 4 0 0 0 0 7 127 222 0.0 4 0 0 0 0 222 140 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 163 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 212 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 276 1 0.0 4 0 0 0 0 1 282 3 0.0 4 0 0 0 0 3 285 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.08 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 18,494 Reads with adapters: 3,662 (19.8%) Reads written (passing filters): 18,494 (100.0%) Total basepairs processed: 5,019,294 bp Total written (filtered): 4,977,678 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3662 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 6.3% G: 89.7% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 24 289.0 0 24 4 4 72.2 0 4 7 1 1.1 0 1 9 15 0.1 0 1 14 10 718 0.0 1 37 681 11 2881 0.0 1 84 2797 12 1 0.0 1 0 1 14 2 0.0 1 0 2 15 1 0.0 1 0 1 151 11 0.0 1 5 6 170 1 0.0 1 1 209 2 0.0 1 2 211 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.