This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 147.06 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 371,323 (11.7%) Reads that were too short: 3,604 (0.1%) Reads written (passing filters): 367,719 (11.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 63,290,720 bp (6.6%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 371323 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 337 773.1 0 337 7 17114 773.1 0 17114 8 22042 773.1 0 22042 9 15 773.1 0 15 10 6 773.1 0 6 11 43 773.1 0 43 12 89 773.1 0 89 13 101 773.1 0 101 14 11 773.1 0 11 15 8 773.1 0 8 16 12 773.1 0 12 17 13 773.1 0 13 18 13 773.1 0 13 19 10 773.1 0 10 20 25 773.1 0 25 21 16 773.1 0 16 22 19 773.1 0 19 23 9 773.1 0 9 24 14 773.1 0 14 25 12 773.1 0 12 26 13 773.1 0 13 27 31 773.1 0 31 28 14 773.1 0 14 29 6 773.1 0 6 30 6 773.1 0 6 31 13 773.1 0 13 32 8 773.1 0 8 33 8 773.1 0 8 34 29 773.1 0 29 35 11 773.1 0 11 36 5 773.1 0 5 37 5 773.1 0 5 38 5 773.1 0 5 39 5 773.1 0 5 40 12 773.1 0 12 41 9 773.1 0 9 42 10 773.1 0 10 43 36 773.1 0 36 44 23 773.1 0 23 45 21 773.1 0 21 46 13 773.1 0 13 47 8 773.1 0 8 48 9 773.1 0 9 49 7 773.1 0 7 50 6 773.1 0 6 51 12 773.1 0 12 52 7 773.1 0 7 53 10 773.1 0 10 54 25 773.1 0 25 55 20 773.1 0 20 56 8 773.1 0 8 57 11 773.1 0 11 58 17 773.1 0 17 59 19 773.1 0 19 60 16 773.1 0 16 61 24 773.1 0 24 62 8 773.1 0 8 63 11 773.1 0 11 64 17 773.1 0 17 65 8 773.1 0 8 66 11 773.1 0 11 67 8 773.1 0 8 68 13 773.1 0 13 69 5 773.1 0 5 70 15 773.1 0 15 71 19 773.1 0 19 72 35 773.1 0 35 73 36 773.1 0 36 74 34 773.1 0 34 75 65 773.1 0 65 76 26 773.1 0 26 77 8 773.1 0 8 78 13 773.1 0 13 79 11 773.1 0 11 80 8 773.1 0 8 81 8 773.1 0 8 82 14 773.1 0 14 83 5 773.1 0 5 84 14 773.1 0 14 85 24 773.1 0 24 86 21 773.1 0 21 87 18 773.1 0 18 88 19 773.1 0 19 89 47 773.1 0 47 90 24 773.1 0 24 91 48 773.1 0 48 92 22 773.1 0 22 93 23 773.1 0 23 94 25 773.1 0 25 95 19 773.1 0 19 96 18 773.1 0 18 97 35 773.1 0 35 98 53 773.1 0 53 99 35 773.1 0 35 100 30 773.1 0 30 101 15 773.1 0 15 102 64 773.1 0 64 103 29 773.1 0 29 104 61 773.1 0 61 105 117 773.1 0 117 106 62 773.1 0 62 107 64 773.1 0 64 108 52 773.1 0 52 109 35 773.1 0 35 110 31 773.1 0 31 111 39 773.1 0 39 112 77 773.1 0 77 113 44 773.1 0 44 114 45 773.1 0 45 115 262 773.1 0 262 116 35 773.1 0 35 117 16 773.1 0 16 118 27 773.1 0 27 119 22 773.1 0 22 120 31 773.1 0 31 121 54 773.1 0 54 122 527 773.1 0 527 123 267 773.1 0 267 124 372 773.1 0 372 125 454 773.1 0 454 126 119 773.1 0 119 127 206 773.1 0 206 128 159 773.1 0 159 129 190 773.1 0 190 130 664 773.1 0 664 131 225 773.1 0 225 132 106 773.1 0 106 133 72 773.1 0 72 134 118 773.1 0 118 135 284 773.1 0 284 136 353 773.1 0 353 137 584 773.1 0 584 138 837 773.1 0 837 139 1253 773.1 0 1253 140 2769 773.1 0 2769 141 5993 773.1 0 5993 142 122599 773.1 0 122599 143 70833 773.1 0 70833 144 92968 773.1 0 92968 145 383 773.1 0 383 146 48 773.1 0 48 147 48 773.1 0 48 148 31 773.1 0 31 149 46 773.1 0 46 150 53 773.1 0 53 151 29 773.1 0 29 152 20 773.1 0 20 153 16 773.1 0 16 154 19 773.1 0 19 155 34 773.1 0 34 156 56 773.1 0 56 157 70 773.1 0 70 158 75 773.1 0 75 159 129 773.1 0 129 160 260 773.1 0 260 161 479 773.1 0 479 162 8772 773.1 0 8772 163 5366 773.1 0 5366 164 6417 773.1 0 6417 165 38 773.1 0 38 166 19 773.1 0 19 167 31 773.1 0 31 168 14 773.1 0 14 169 27 773.1 0 27 170 16 773.1 0 16 171 7 773.1 0 7 172 6 773.1 0 6 173 2 773.1 0 2 174 9 773.1 0 9 175 12 773.1 0 12 176 24 773.1 0 24 177 39 773.1 0 39 178 25 773.1 0 25 179 10 773.1 0 10 180 9 773.1 0 9 181 12 773.1 0 12 182 7 773.1 0 7 183 16 773.1 0 16 184 18 773.1 0 18 185 19 773.1 0 19 186 11 773.1 0 11 187 16 773.1 0 16 188 182 773.1 0 182 189 85 773.1 0 85 190 191 773.1 0 191 191 6 773.1 0 6 192 11 773.1 0 11 193 6 773.1 0 6 194 10 773.1 0 10 195 19 773.1 0 19 196 20 773.1 0 20 197 20 773.1 0 20 198 11 773.1 0 11 199 14 773.1 0 14 200 16 773.1 0 16 201 12 773.1 0 12 202 18 773.1 0 18 203 20 773.1 0 20 204 19 773.1 0 19 205 18 773.1 0 18 206 21 773.1 0 21 207 17 773.1 0 17 208 31 773.1 0 31 209 21 773.1 0 21 210 39 773.1 0 39 211 32 773.1 0 32 212 31 773.1 0 31 213 27 773.1 0 27 214 123 773.1 0 123 215 156 773.1 0 156 216 132 773.1 0 132 217 51 773.1 0 51 218 12 773.1 0 12 219 17 773.1 0 17 220 11 773.1 0 11 221 15 773.1 0 15 222 16 773.1 0 16 223 35 773.1 0 35 224 38 773.1 0 38 225 31 773.1 0 31 226 31 773.1 0 31 227 49 773.1 0 49 228 21 773.1 0 21 229 19 773.1 0 19 230 8 773.1 0 8 231 11 773.1 0 11 232 27 773.1 0 27 233 36 773.1 0 36 234 38 773.1 0 38 235 20 773.1 0 20 236 22 773.1 0 22 237 21 773.1 0 21 238 22 773.1 0 22 239 26 773.1 0 26 240 27 773.1 0 27 241 24 773.1 0 24 242 18 773.1 0 18 243 26 773.1 0 26 244 25 773.1 0 25 245 29 773.1 0 29 246 19 773.1 0 19 247 20 773.1 0 20 248 15 773.1 0 15 249 15 773.1 0 15 250 18 773.1 0 18 251 19 773.1 0 19 252 17 773.1 0 17 253 20 773.1 0 20 254 21 773.1 0 21 255 42 773.1 0 42 256 37 773.1 0 37 257 48 773.1 0 48 258 31 773.1 0 31 259 34 773.1 0 34 260 36 773.1 0 36 261 145 773.1 0 145 262 140 773.1 0 140 263 198 773.1 0 198 264 37 773.1 0 37 265 14 773.1 0 14 266 12 773.1 0 12 267 22 773.1 0 22 268 17 773.1 0 17 269 35 773.1 0 35 270 20 773.1 0 20 271 32 773.1 0 32 272 7 773.1 0 7 273 26 773.1 0 26 274 17 773.1 0 17 275 24 773.1 0 24 276 33 773.1 0 33 277 33 773.1 0 33 278 30 773.1 0 30 279 93 773.1 0 93 280 71 773.1 0 71 281 143 773.1 0 143 282 38 773.1 0 38 283 45 773.1 0 45 284 33 773.1 0 33 285 38 773.1 0 38 286 18 773.1 0 18 287 31 773.1 0 31 288 24 773.1 0 24 289 20 773.1 0 20 290 37 773.1 0 37 291 18 773.1 0 18 292 33 773.1 0 33 293 25 773.1 0 25 294 33 773.1 0 33 295 39 773.1 0 39 296 56 773.1 0 56 297 57 773.1 0 57 298 34 773.1 0 34 299 17 773.1 0 17 300 22 773.1 0 22 301 14 773.1 0 14 Finished in 19.20 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 367,719 Reads with adapters: 16,998 (4.6%) Reads that were too short: 21 (0.0%) Reads written (passing filters): 16,977 (4.6%) Total basepairs processed: 63,290,720 bp Total written (filtered): 4,701,271 bp (7.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 16998 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 69 0.0 3 0 66 3 17 16852 0.0 3 16571 273 8 18 29 0.0 3 0 29 19 10 0.0 3 2 0 7 1 20 5 0.0 3 0 0 0 5 35 1 0.0 3 0 1 55 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 59 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 1 96 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 1 134 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 146 4 0.0 3 0 0 0 4 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 217 7 0.0 3 0 0 0 7 239 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.93 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 16,977 Reads with adapters: 37 (0.2%) Reads written (passing filters): 16,977 (100.0%) Total basepairs processed: 4,701,271 bp Total written (filtered): 4,701,151 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 37 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.9% C: 67.6% G: 2.7% T: 10.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 32 265.3 0 32 4 3 66.3 0 3 6 2 4.1 0 2