This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 145.55 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 224,696 (7.1%) Reads that were too short: 20,994 (0.7%) Reads written (passing filters): 203,702 (6.4%) Total basepairs processed: 953,096,634 bp Total written (filtered): 54,759,039 bp (5.7%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 224696 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1246 773.1 0 1246 7 77387 773.1 0 77387 8 1640 773.1 0 1640 9 86081 773.1 0 86081 10 162 773.1 0 162 11 62 773.1 0 62 12 93 773.1 0 93 13 63 773.1 0 63 14 53 773.1 0 53 15 70 773.1 0 70 16 66 773.1 0 66 17 66 773.1 0 66 18 50 773.1 0 50 19 66 773.1 0 66 20 81 773.1 0 81 21 71 773.1 0 71 22 73 773.1 0 73 23 79 773.1 0 79 24 84 773.1 0 84 25 77 773.1 0 77 26 62 773.1 0 62 27 78 773.1 0 78 28 81 773.1 0 81 29 97 773.1 0 97 30 79 773.1 0 79 31 219 773.1 0 219 32 327 773.1 0 327 33 296 773.1 0 296 34 69 773.1 0 69 35 60 773.1 0 60 36 59 773.1 0 59 37 87 773.1 0 87 38 71 773.1 0 71 39 68 773.1 0 68 40 59 773.1 0 59 41 78 773.1 0 78 42 56 773.1 0 56 43 63 773.1 0 63 44 61 773.1 0 61 45 61 773.1 0 61 46 85 773.1 0 85 47 63 773.1 0 63 48 74 773.1 0 74 49 58 773.1 0 58 50 92 773.1 0 92 51 94 773.1 0 94 52 120 773.1 0 120 53 56 773.1 0 56 54 52 773.1 0 52 55 72 773.1 0 72 56 68 773.1 0 68 57 51 773.1 0 51 58 71 773.1 0 71 59 74 773.1 0 74 60 51 773.1 0 51 61 49 773.1 0 49 62 73 773.1 0 73 63 73 773.1 0 73 64 72 773.1 0 72 65 79 773.1 0 79 66 78 773.1 0 78 67 94 773.1 0 94 68 415 773.1 0 415 69 552 773.1 0 552 70 762 773.1 0 762 71 51 773.1 0 51 72 71 773.1 0 71 73 93 773.1 0 93 74 88 773.1 0 88 75 80 773.1 0 80 76 52 773.1 0 52 77 61 773.1 0 61 78 64 773.1 0 64 79 119 773.1 0 119 80 98 773.1 0 98 81 87 773.1 0 87 82 124 773.1 0 124 83 88 773.1 0 88 84 96 773.1 0 96 85 68 773.1 0 68 86 87 773.1 0 87 87 78 773.1 0 78 88 101 773.1 0 101 89 80 773.1 0 80 90 63 773.1 0 63 91 68 773.1 0 68 92 72 773.1 0 72 93 73 773.1 0 73 94 80 773.1 0 80 95 63 773.1 0 63 96 62 773.1 0 62 97 70 773.1 0 70 98 148 773.1 0 148 99 114 773.1 0 114 100 140 773.1 0 140 101 81 773.1 0 81 102 76 773.1 0 76 103 53 773.1 0 53 104 63 773.1 0 63 105 55 773.1 0 55 106 67 773.1 0 67 107 74 773.1 0 74 108 66 773.1 0 66 109 70 773.1 0 70 110 85 773.1 0 85 111 76 773.1 0 76 112 78 773.1 0 78 113 89 773.1 0 89 114 76 773.1 0 76 115 67 773.1 0 67 116 387 773.1 0 387 117 253 773.1 0 253 118 147 773.1 0 147 119 60 773.1 0 60 120 101 773.1 0 101 121 182 773.1 0 182 122 167 773.1 0 167 123 181 773.1 0 181 124 128 773.1 0 128 125 92 773.1 0 92 126 97 773.1 0 97 127 102 773.1 0 102 128 106 773.1 0 106 129 128 773.1 0 128 130 141 773.1 0 141 131 193 773.1 0 193 132 155 773.1 0 155 133 745 773.1 0 745 134 810 773.1 0 810 135 1106 773.1 0 1106 136 355 773.1 0 355 137 220 773.1 0 220 138 234 773.1 0 234 139 320 773.1 0 320 140 62 773.1 0 62 141 88 773.1 0 88 142 150 773.1 0 150 143 80 773.1 0 80 144 136 773.1 0 136 145 119 773.1 0 119 146 183 773.1 0 183 147 193 773.1 0 193 148 143 773.1 0 143 149 87 773.1 0 87 150 179 773.1 0 179 151 356 773.1 0 356 152 659 773.1 0 659 153 312 773.1 0 312 154 364 773.1 0 364 155 260 773.1 0 260 156 195 773.1 0 195 157 212 773.1 0 212 158 104 773.1 0 104 159 111 773.1 0 111 160 231 773.1 0 231 161 80 773.1 0 80 162 140 773.1 0 140 163 111 773.1 0 111 164 133 773.1 0 133 165 176 773.1 0 176 166 101 773.1 0 101 167 243 773.1 0 243 168 164 773.1 0 164 169 153 773.1 0 153 170 102 773.1 0 102 171 80 773.1 0 80 172 67 773.1 0 67 173 77 773.1 0 77 174 180 773.1 0 180 175 588 773.1 0 588 176 443 773.1 0 443 177 605 773.1 0 605 178 217 773.1 0 217 179 201 773.1 0 201 180 851 773.1 0 851 181 1026 773.1 0 1026 182 1341 773.1 0 1341 183 418 773.1 0 418 184 144 773.1 0 144 185 96 773.1 0 96 186 89 773.1 0 89 187 139 773.1 0 139 188 120 773.1 0 120 189 103 773.1 0 103 190 94 773.1 0 94 191 80 773.1 0 80 192 108 773.1 0 108 193 133 773.1 0 133 194 214 773.1 0 214 195 711 773.1 0 711 196 863 773.1 0 863 197 758 773.1 0 758 198 286 773.1 0 286 199 137 773.1 0 137 200 1916 773.1 0 1916 201 3036 773.1 0 3036 202 2281 773.1 0 2281 203 692 773.1 0 692 204 59 773.1 0 59 205 74 773.1 0 74 206 62 773.1 0 62 207 88 773.1 0 88 208 132 773.1 0 132 209 76 773.1 0 76 210 86 773.1 0 86 211 63 773.1 0 63 212 85 773.1 0 85 213 114 773.1 0 114 214 91 773.1 0 91 215 90 773.1 0 90 216 77 773.1 0 77 217 73 773.1 0 73 218 91 773.1 0 91 219 77 773.1 0 77 220 79 773.1 0 79 221 324 773.1 0 324 222 295 773.1 0 295 223 212 773.1 0 212 224 124 773.1 0 124 225 88 773.1 0 88 226 100 773.1 0 100 227 105 773.1 0 105 228 61 773.1 0 61 229 61 773.1 0 61 230 77 773.1 0 77 231 82 773.1 0 82 232 76 773.1 0 76 233 93 773.1 0 93 234 64 773.1 0 64 235 93 773.1 0 93 236 94 773.1 0 94 237 84 773.1 0 84 238 84 773.1 0 84 239 149 773.1 0 149 240 121 773.1 0 121 241 180 773.1 0 180 242 200 773.1 0 200 243 133 773.1 0 133 244 102 773.1 0 102 245 80 773.1 0 80 246 68 773.1 0 68 247 75 773.1 0 75 248 104 773.1 0 104 249 307 773.1 0 307 250 262 773.1 0 262 251 423 773.1 0 423 252 151 773.1 0 151 253 266 773.1 0 266 254 225 773.1 0 225 255 295 773.1 0 295 256 201 773.1 0 201 257 205 773.1 0 205 258 409 773.1 0 409 259 228 773.1 0 228 260 369 773.1 0 369 261 136 773.1 0 136 262 159 773.1 0 159 263 256 773.1 0 256 264 1070 773.1 0 1070 265 959 773.1 0 959 266 989 773.1 0 989 267 114 773.1 0 114 268 149 773.1 0 149 269 164 773.1 0 164 270 105 773.1 0 105 271 87 773.1 0 87 272 102 773.1 0 102 273 148 773.1 0 148 274 188 773.1 0 188 275 257 773.1 0 257 276 902 773.1 0 902 277 712 773.1 0 712 278 892 773.1 0 892 279 64 773.1 0 64 280 79 773.1 0 79 281 53 773.1 0 53 282 70 773.1 0 70 283 89 773.1 0 89 284 68 773.1 0 68 285 84 773.1 0 84 286 86 773.1 0 86 287 88 773.1 0 88 288 75 773.1 0 75 289 104 773.1 0 104 290 144 773.1 0 144 291 131 773.1 0 131 292 126 773.1 0 126 293 86 773.1 0 86 294 120 773.1 0 120 295 105 773.1 0 105 296 116 773.1 0 116 297 127 773.1 0 127 298 156 773.1 0 156 299 123 773.1 0 123 300 162 773.1 0 162 301 289 773.1 0 289 Finished in 14.99 s (74 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 203,702 Reads with adapters: 77,343 (38.0%) Reads that were too short: 77 (0.0%) Reads written (passing filters): 77,266 (37.9%) Total basepairs processed: 54,759,039 bp Total written (filtered): 21,156,268 bp (38.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 77343 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 7 0.0 3 0 0 3 4 19 374 0.0 3 0 278 60 23 13 20 76433 0.0 4 73153 2730 424 100 26 21 169 0.0 4 31 130 6 2 22 7 0.0 4 1 0 3 0 3 23 133 0.0 4 0 1 0 96 36 25 3 0.0 4 0 1 0 0 2 27 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 30 2 0.0 4 0 0 0 0 2 31 1 0.0 4 0 0 0 0 1 32 2 0.0 4 0 0 0 0 2 35 1 0.0 4 0 0 0 0 1 40 5 0.0 4 0 0 0 1 4 41 2 0.0 4 0 0 0 1 1 42 2 0.0 4 0 0 0 0 2 43 1 0.0 4 1 47 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 64 2 0.0 4 0 0 0 0 2 70 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 87 1 0.0 4 1 89 2 0.0 4 0 0 0 0 2 91 3 0.0 4 0 0 0 0 3 92 2 0.0 4 0 0 0 0 2 93 1 0.0 4 0 0 0 0 1 103 1 0.0 4 1 109 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 4 0.0 4 0 0 0 0 4 127 119 0.0 4 0 0 0 0 119 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 172 2 0.0 4 0 1 0 0 1 178 1 0.0 4 0 0 0 0 1 183 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 205 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 3 0.0 4 0 0 0 0 3 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 3 0.0 4 0 0 0 0 3 236 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 260 2 0.0 4 0 0 0 0 2 262 3 0.0 4 0 0 0 0 3 263 1 0.0 4 0 0 0 0 1 273 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 282 7 0.0 4 0 0 0 0 7 283 3 0.0 4 0 0 0 0 3 284 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.49 s (58 us/read; 1.03 M reads/minute). === Summary === Total reads processed: 77,266 Reads with adapters: 9,678 (12.5%) Reads written (passing filters): 77,266 (100.0%) Total basepairs processed: 21,156,268 bp Total written (filtered): 21,039,625 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 9678 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.9% C: 4.7% G: 90.8% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 207 1207.3 0 207 4 33 301.8 0 33 5 6 75.5 0 6 6 4 18.9 0 4 7 1 4.7 0 1 8 2 1.2 0 2 9 23 0.3 0 3 20 10 38 0.1 1 1 37 11 8731 0.0 1 156 8575 12 544 0.0 1 22 522 13 7 0.0 1 0 7 14 3 0.0 1 0 3 15 6 0.0 1 0 6 41 1 0.0 1 1 42 1 0.0 1 1 93 1 0.0 1 1 130 1 0.0 1 1 139 1 0.0 1 0 1 149 2 0.0 1 1 1 152 37 0.0 1 21 16 176 2 0.0 1 2 179 1 0.0 1 1 191 2 0.0 1 0 2 210 15 0.0 1 14 1 211 1 0.0 1 1 213 1 0.0 1 1 220 1 0.0 1 0 1 222 1 0.0 1 0 1 225 1 0.0 1 1 228 3 0.0 1 3 229 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.