This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 145.26 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 223,583 (7.1%) Reads that were too short: 27,783 (0.9%) Reads written (passing filters): 195,800 (6.2%) Total basepairs processed: 953,096,634 bp Total written (filtered): 53,991,925 bp (5.7%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 223583 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 866 773.1 0 866 7 29908 773.1 0 29908 8 925 773.1 0 925 9 23533 773.1 0 23533 10 899 773.1 0 899 11 908 773.1 0 908 12 16960 773.1 0 16960 13 21640 773.1 0 21640 14 77669 773.1 0 77669 15 158 773.1 0 158 16 43 773.1 0 43 17 36 773.1 0 36 18 33 773.1 0 33 19 44 773.1 0 44 20 30 773.1 0 30 21 34 773.1 0 34 22 45 773.1 0 45 23 41 773.1 0 41 24 28 773.1 0 28 25 28 773.1 0 28 26 75 773.1 0 75 27 111 773.1 0 111 28 74 773.1 0 74 29 39 773.1 0 39 30 40 773.1 0 40 31 29 773.1 0 29 32 43 773.1 0 43 33 38 773.1 0 38 34 35 773.1 0 35 35 41 773.1 0 41 36 33 773.1 0 33 37 36 773.1 0 36 38 44 773.1 0 44 39 40 773.1 0 40 40 62 773.1 0 62 41 42 773.1 0 42 42 56 773.1 0 56 43 90 773.1 0 90 44 144 773.1 0 144 45 135 773.1 0 135 46 35 773.1 0 35 47 30 773.1 0 30 48 45 773.1 0 45 49 50 773.1 0 50 50 40 773.1 0 40 51 25 773.1 0 25 52 34 773.1 0 34 53 40 773.1 0 40 54 40 773.1 0 40 55 26 773.1 0 26 56 42 773.1 0 42 57 55 773.1 0 55 58 51 773.1 0 51 59 54 773.1 0 54 60 29 773.1 0 29 61 40 773.1 0 40 62 40 773.1 0 40 63 46 773.1 0 46 64 39 773.1 0 39 65 38 773.1 0 38 66 46 773.1 0 46 67 49 773.1 0 49 68 34 773.1 0 34 69 36 773.1 0 36 70 39 773.1 0 39 71 34 773.1 0 34 72 26 773.1 0 26 73 59 773.1 0 59 74 42 773.1 0 42 75 39 773.1 0 39 76 39 773.1 0 39 77 40 773.1 0 40 78 71 773.1 0 71 79 65 773.1 0 65 80 52 773.1 0 52 81 42 773.1 0 42 82 36 773.1 0 36 83 36 773.1 0 36 84 49 773.1 0 49 85 49 773.1 0 49 86 44 773.1 0 44 87 53 773.1 0 53 88 36 773.1 0 36 89 61 773.1 0 61 90 54 773.1 0 54 91 47 773.1 0 47 92 125 773.1 0 125 93 39 773.1 0 39 94 56 773.1 0 56 95 42 773.1 0 42 96 51 773.1 0 51 97 39 773.1 0 39 98 77 773.1 0 77 99 54 773.1 0 54 100 30 773.1 0 30 101 46 773.1 0 46 102 57 773.1 0 57 103 51 773.1 0 51 104 361 773.1 0 361 105 306 773.1 0 306 106 557 773.1 0 557 107 65 773.1 0 65 108 126 773.1 0 126 109 70 773.1 0 70 110 124 773.1 0 124 111 97 773.1 0 97 112 113 773.1 0 113 113 118 773.1 0 118 114 79 773.1 0 79 115 76 773.1 0 76 116 81 773.1 0 81 117 85 773.1 0 85 118 39 773.1 0 39 119 49 773.1 0 49 120 76 773.1 0 76 121 85 773.1 0 85 122 66 773.1 0 66 123 72 773.1 0 72 124 111 773.1 0 111 125 300 773.1 0 300 126 554 773.1 0 554 127 452 773.1 0 452 128 462 773.1 0 462 129 198 773.1 0 198 130 65 773.1 0 65 131 52 773.1 0 52 132 64 773.1 0 64 133 89 773.1 0 89 134 105 773.1 0 105 135 148 773.1 0 148 136 117 773.1 0 117 137 371 773.1 0 371 138 541 773.1 0 541 139 810 773.1 0 810 140 978 773.1 0 978 141 464 773.1 0 464 142 513 773.1 0 513 143 75 773.1 0 75 144 48 773.1 0 48 145 68 773.1 0 68 146 78 773.1 0 78 147 104 773.1 0 104 148 49 773.1 0 49 149 52 773.1 0 52 150 59 773.1 0 59 151 259 773.1 0 259 152 242 773.1 0 242 153 249 773.1 0 249 154 90 773.1 0 90 155 101 773.1 0 101 156 99 773.1 0 99 157 129 773.1 0 129 158 1208 773.1 0 1208 159 1081 773.1 0 1081 160 1121 773.1 0 1121 161 353 773.1 0 353 162 108 773.1 0 108 163 145 773.1 0 145 164 61 773.1 0 61 165 65 773.1 0 65 166 54 773.1 0 54 167 94 773.1 0 94 168 76 773.1 0 76 169 131 773.1 0 131 170 166 773.1 0 166 171 101 773.1 0 101 172 40 773.1 0 40 173 70 773.1 0 70 174 92 773.1 0 92 175 101 773.1 0 101 176 89 773.1 0 89 177 76 773.1 0 76 178 40 773.1 0 40 179 63 773.1 0 63 180 71 773.1 0 71 181 171 773.1 0 171 182 193 773.1 0 193 183 169 773.1 0 169 184 97 773.1 0 97 185 53 773.1 0 53 186 62 773.1 0 62 187 73 773.1 0 73 188 204 773.1 0 204 189 121 773.1 0 121 190 124 773.1 0 124 191 42 773.1 0 42 192 60 773.1 0 60 193 49 773.1 0 49 194 61 773.1 0 61 195 40 773.1 0 40 196 58 773.1 0 58 197 59 773.1 0 59 198 63 773.1 0 63 199 89 773.1 0 89 200 97 773.1 0 97 201 89 773.1 0 89 202 83 773.1 0 83 203 66 773.1 0 66 204 60 773.1 0 60 205 55 773.1 0 55 206 74 773.1 0 74 207 107 773.1 0 107 208 113 773.1 0 113 209 80 773.1 0 80 210 46 773.1 0 46 211 72 773.1 0 72 212 117 773.1 0 117 213 99 773.1 0 99 214 122 773.1 0 122 215 155 773.1 0 155 216 123 773.1 0 123 217 65 773.1 0 65 218 86 773.1 0 86 219 98 773.1 0 98 220 490 773.1 0 490 221 331 773.1 0 331 222 279 773.1 0 279 223 183 773.1 0 183 224 1290 773.1 0 1290 225 667 773.1 0 667 226 3695 773.1 0 3695 227 94 773.1 0 94 228 69 773.1 0 69 229 52 773.1 0 52 230 92 773.1 0 92 231 111 773.1 0 111 232 165 773.1 0 165 233 295 773.1 0 295 234 278 773.1 0 278 235 216 773.1 0 216 236 162 773.1 0 162 237 98 773.1 0 98 238 62 773.1 0 62 239 66 773.1 0 66 240 97 773.1 0 97 241 133 773.1 0 133 242 178 773.1 0 178 243 90 773.1 0 90 244 80 773.1 0 80 245 59 773.1 0 59 246 36 773.1 0 36 247 68 773.1 0 68 248 47 773.1 0 47 249 41 773.1 0 41 250 64 773.1 0 64 251 88 773.1 0 88 252 89 773.1 0 89 253 95 773.1 0 95 254 101 773.1 0 101 255 71 773.1 0 71 256 102 773.1 0 102 257 88 773.1 0 88 258 107 773.1 0 107 259 52 773.1 0 52 260 97 773.1 0 97 261 94 773.1 0 94 262 106 773.1 0 106 263 84 773.1 0 84 264 66 773.1 0 66 265 78 773.1 0 78 266 65 773.1 0 65 267 361 773.1 0 361 268 337 773.1 0 337 269 391 773.1 0 391 270 141 773.1 0 141 271 105 773.1 0 105 272 130 773.1 0 130 273 111 773.1 0 111 274 102 773.1 0 102 275 77 773.1 0 77 276 151 773.1 0 151 277 356 773.1 0 356 278 431 773.1 0 431 279 405 773.1 0 405 280 691 773.1 0 691 281 2377 773.1 0 2377 282 2848 773.1 0 2848 283 2959 773.1 0 2959 284 883 773.1 0 883 285 62 773.1 0 62 286 90 773.1 0 90 287 122 773.1 0 122 288 340 773.1 0 340 289 271 773.1 0 271 290 418 773.1 0 418 291 121 773.1 0 121 292 96 773.1 0 96 293 84 773.1 0 84 294 116 773.1 0 116 295 161 773.1 0 161 296 147 773.1 0 147 297 74 773.1 0 74 298 58 773.1 0 58 299 36 773.1 0 36 300 60 773.1 0 60 301 79 773.1 0 79 Finished in 14.22 s (73 us/read; 0.83 M reads/minute). === Summary === Total reads processed: 195,800 Reads with adapters: 24,603 (12.6%) Reads that were too short: 98 (0.1%) Reads written (passing filters): 24,505 (12.5%) Total basepairs processed: 53,991,925 bp Total written (filtered): 6,619,625 bp (12.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 24603 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 1 0.0 3 0 0 0 1 18 7 0.0 3 0 0 2 1 4 19 94 0.0 3 0 70 12 8 4 20 23820 0.0 4 22563 995 199 42 21 21 59 0.0 4 13 35 5 3 3 22 3 0.0 4 0 0 2 0 1 23 74 0.0 4 3 0 0 49 22 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 34 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 47 2 0.0 4 1 0 0 0 1 65 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 1 87 3 0.0 4 0 0 0 0 3 92 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 109 4 0.0 4 0 0 0 0 4 110 2 0.0 4 0 0 0 0 2 117 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 121 9 0.0 4 0 0 0 0 9 122 357 0.0 4 0 0 0 0 357 123 2 0.0 4 0 0 0 0 2 126 1 0.0 4 0 0 0 0 1 127 62 0.0 4 0 0 0 0 62 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 134 2 0.0 4 0 0 0 0 2 141 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 148 3 0.0 4 0 0 0 0 3 153 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 198 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 208 3 0.0 4 0 0 0 0 3 209 5 0.0 4 0 0 0 0 5 210 2 0.0 4 0 0 0 0 2 211 1 0.0 4 0 0 0 0 1 220 1 0.0 4 0 0 0 0 1 223 5 0.0 4 0 0 0 0 5 224 7 0.0 4 0 0 0 0 7 227 2 0.0 4 0 0 0 0 2 228 4 0.0 4 0 0 0 0 4 229 8 0.0 4 0 0 0 0 8 230 2 0.0 4 0 0 0 0 2 232 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 252 2 0.0 4 0 0 0 0 2 255 2 0.0 4 0 0 0 0 2 257 3 0.0 4 0 0 0 0 3 258 2 0.0 4 0 0 0 0 2 259 2 0.0 4 0 0 0 0 2 268 1 0.0 4 0 0 0 0 1 270 1 0.0 4 0 0 0 0 1 276 2 0.0 4 0 0 0 0 2 277 9 0.0 4 0 0 0 0 9 278 1 0.0 4 0 0 0 0 1 279 2 0.0 4 0 0 0 0 2 288 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.48 s (60 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 24,505 Reads with adapters: 6,587 (26.9%) Reads written (passing filters): 24,505 (100.0%) Total basepairs processed: 6,619,625 bp Total written (filtered): 6,553,971 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6587 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 5.0% G: 92.2% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 9 382.9 0 9 4 2 95.7 0 2 6 2 6.0 0 2 8 2 0.4 0 2 9 3085 0.1 0 185 2900 10 2799 0.0 1 177 2622 11 638 0.0 1 15 623 12 21 0.0 1 0 21 13 13 0.0 1 0 13 19 2 0.0 1 0 2 40 1 0.0 1 0 1 150 6 0.0 1 5 1 153 1 0.0 1 0 1 208 4 0.0 1 2 2 211 1 0.0 1 1 223 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.