This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 146.90 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 193,518 (6.1%) Reads that were too short: 18,674 (0.6%) Reads written (passing filters): 174,844 (5.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 48,887,790 bp (5.1%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 193518 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 778 773.1 0 778 7 25028 773.1 0 25028 8 502 773.1 0 502 9 30099 773.1 0 30099 10 708 773.1 0 708 11 694 773.1 0 694 12 14704 773.1 0 14704 13 19845 773.1 0 19845 14 67687 773.1 0 67687 15 109 773.1 0 109 16 23 773.1 0 23 17 26 773.1 0 26 18 31 773.1 0 31 19 18 773.1 0 18 20 27 773.1 0 27 21 27 773.1 0 27 22 18 773.1 0 18 23 26 773.1 0 26 24 35 773.1 0 35 25 36 773.1 0 36 26 31 773.1 0 31 27 27 773.1 0 27 28 22 773.1 0 22 29 31 773.1 0 31 30 20 773.1 0 20 31 21 773.1 0 21 32 32 773.1 0 32 33 27 773.1 0 27 34 19 773.1 0 19 35 28 773.1 0 28 36 19 773.1 0 19 37 24 773.1 0 24 38 28 773.1 0 28 39 37 773.1 0 37 40 40 773.1 0 40 41 29 773.1 0 29 42 20 773.1 0 20 43 59 773.1 0 59 44 92 773.1 0 92 45 120 773.1 0 120 46 24 773.1 0 24 47 20 773.1 0 20 48 35 773.1 0 35 49 25 773.1 0 25 50 30 773.1 0 30 51 32 773.1 0 32 52 21 773.1 0 21 53 43 773.1 0 43 54 33 773.1 0 33 55 19 773.1 0 19 56 15 773.1 0 15 57 33 773.1 0 33 58 39 773.1 0 39 59 25 773.1 0 25 60 20 773.1 0 20 61 27 773.1 0 27 62 23 773.1 0 23 63 37 773.1 0 37 64 30 773.1 0 30 65 24 773.1 0 24 66 37 773.1 0 37 67 35 773.1 0 35 68 28 773.1 0 28 69 21 773.1 0 21 70 38 773.1 0 38 71 26 773.1 0 26 72 32 773.1 0 32 73 37 773.1 0 37 74 33 773.1 0 33 75 24 773.1 0 24 76 36 773.1 0 36 77 33 773.1 0 33 78 47 773.1 0 47 79 42 773.1 0 42 80 35 773.1 0 35 81 34 773.1 0 34 82 48 773.1 0 48 83 45 773.1 0 45 84 32 773.1 0 32 85 52 773.1 0 52 86 43 773.1 0 43 87 33 773.1 0 33 88 44 773.1 0 44 89 57 773.1 0 57 90 52 773.1 0 52 91 45 773.1 0 45 92 104 773.1 0 104 93 40 773.1 0 40 94 61 773.1 0 61 95 50 773.1 0 50 96 40 773.1 0 40 97 40 773.1 0 40 98 51 773.1 0 51 99 40 773.1 0 40 100 39 773.1 0 39 101 29 773.1 0 29 102 44 773.1 0 44 103 47 773.1 0 47 104 257 773.1 0 257 105 215 773.1 0 215 106 390 773.1 0 390 107 52 773.1 0 52 108 63 773.1 0 63 109 52 773.1 0 52 110 93 773.1 0 93 111 65 773.1 0 65 112 65 773.1 0 65 113 85 773.1 0 85 114 61 773.1 0 61 115 48 773.1 0 48 116 64 773.1 0 64 117 52 773.1 0 52 118 35 773.1 0 35 119 46 773.1 0 46 120 66 773.1 0 66 121 55 773.1 0 55 122 58 773.1 0 58 123 41 773.1 0 41 124 78 773.1 0 78 125 133 773.1 0 133 126 420 773.1 0 420 127 341 773.1 0 341 128 376 773.1 0 376 129 161 773.1 0 161 130 54 773.1 0 54 131 44 773.1 0 44 132 53 773.1 0 53 133 56 773.1 0 56 134 74 773.1 0 74 135 133 773.1 0 133 136 85 773.1 0 85 137 315 773.1 0 315 138 180 773.1 0 180 139 376 773.1 0 376 140 514 773.1 0 514 141 325 773.1 0 325 142 368 773.1 0 368 143 58 773.1 0 58 144 41 773.1 0 41 145 59 773.1 0 59 146 56 773.1 0 56 147 50 773.1 0 50 148 49 773.1 0 49 149 41 773.1 0 41 150 48 773.1 0 48 151 244 773.1 0 244 152 197 773.1 0 197 153 180 773.1 0 180 154 45 773.1 0 45 155 70 773.1 0 70 156 68 773.1 0 68 157 70 773.1 0 70 158 656 773.1 0 656 159 541 773.1 0 541 160 633 773.1 0 633 161 188 773.1 0 188 162 57 773.1 0 57 163 69 773.1 0 69 164 48 773.1 0 48 165 53 773.1 0 53 166 44 773.1 0 44 167 54 773.1 0 54 168 45 773.1 0 45 169 63 773.1 0 63 170 142 773.1 0 142 171 114 773.1 0 114 172 38 773.1 0 38 173 36 773.1 0 36 174 72 773.1 0 72 175 65 773.1 0 65 176 54 773.1 0 54 177 37 773.1 0 37 178 38 773.1 0 38 179 54 773.1 0 54 180 35 773.1 0 35 181 99 773.1 0 99 182 65 773.1 0 65 183 61 773.1 0 61 184 57 773.1 0 57 185 45 773.1 0 45 186 59 773.1 0 59 187 57 773.1 0 57 188 102 773.1 0 102 189 76 773.1 0 76 190 60 773.1 0 60 191 36 773.1 0 36 192 38 773.1 0 38 193 42 773.1 0 42 194 50 773.1 0 50 195 44 773.1 0 44 196 38 773.1 0 38 197 51 773.1 0 51 198 55 773.1 0 55 199 56 773.1 0 56 200 50 773.1 0 50 201 66 773.1 0 66 202 54 773.1 0 54 203 63 773.1 0 63 204 50 773.1 0 50 205 36 773.1 0 36 206 45 773.1 0 45 207 67 773.1 0 67 208 66 773.1 0 66 209 67 773.1 0 67 210 47 773.1 0 47 211 42 773.1 0 42 212 100 773.1 0 100 213 36 773.1 0 36 214 128 773.1 0 128 215 117 773.1 0 117 216 98 773.1 0 98 217 74 773.1 0 74 218 76 773.1 0 76 219 68 773.1 0 68 220 310 773.1 0 310 221 247 773.1 0 247 222 234 773.1 0 234 223 172 773.1 0 172 224 1354 773.1 0 1354 225 593 773.1 0 593 226 3960 773.1 0 3960 227 73 773.1 0 73 228 38 773.1 0 38 229 46 773.1 0 46 230 46 773.1 0 46 231 48 773.1 0 48 232 108 773.1 0 108 233 202 773.1 0 202 234 255 773.1 0 255 235 200 773.1 0 200 236 112 773.1 0 112 237 81 773.1 0 81 238 47 773.1 0 47 239 49 773.1 0 49 240 79 773.1 0 79 241 97 773.1 0 97 242 118 773.1 0 118 243 70 773.1 0 70 244 69 773.1 0 69 245 45 773.1 0 45 246 33 773.1 0 33 247 49 773.1 0 49 248 43 773.1 0 43 249 51 773.1 0 51 250 48 773.1 0 48 251 79 773.1 0 79 252 71 773.1 0 71 253 67 773.1 0 67 254 46 773.1 0 46 255 48 773.1 0 48 256 74 773.1 0 74 257 84 773.1 0 84 258 71 773.1 0 71 259 51 773.1 0 51 260 63 773.1 0 63 261 69 773.1 0 69 262 78 773.1 0 78 263 77 773.1 0 77 264 54 773.1 0 54 265 52 773.1 0 52 266 60 773.1 0 60 267 203 773.1 0 203 268 254 773.1 0 254 269 245 773.1 0 245 270 92 773.1 0 92 271 85 773.1 0 85 272 90 773.1 0 90 273 60 773.1 0 60 274 52 773.1 0 52 275 53 773.1 0 53 276 142 773.1 0 142 277 322 773.1 0 322 278 413 773.1 0 413 279 421 773.1 0 421 280 307 773.1 0 307 281 823 773.1 0 823 282 925 773.1 0 925 283 1145 773.1 0 1145 284 330 773.1 0 330 285 55 773.1 0 55 286 53 773.1 0 53 287 79 773.1 0 79 288 201 773.1 0 201 289 168 773.1 0 168 290 251 773.1 0 251 291 82 773.1 0 82 292 87 773.1 0 87 293 61 773.1 0 61 294 82 773.1 0 82 295 98 773.1 0 98 296 100 773.1 0 100 297 49 773.1 0 49 298 47 773.1 0 47 299 40 773.1 0 40 300 41 773.1 0 41 301 63 773.1 0 63 Finished in 11.83 s (68 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 174,844 Reads with adapters: 25,418 (14.5%) Reads that were too short: 103 (0.1%) Reads written (passing filters): 25,315 (14.5%) Total basepairs processed: 48,887,790 bp Total written (filtered): 6,911,507 bp (14.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25418 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 84 0.0 3 0 81 3 17 24316 0.0 3 23905 396 15 18 43 0.0 3 3 40 19 14 0.0 3 0 1 11 2 20 188 0.0 3 171 8 0 9 21 1 0.0 3 0 1 23 2 0.0 3 0 0 1 1 26 1 0.0 3 1 31 2 0.0 3 0 0 0 2 32 12 0.0 3 0 0 0 12 33 2 0.0 3 1 0 0 1 34 1 0.0 3 0 0 0 1 35 3 0.0 3 1 0 0 2 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 7 0.0 3 0 0 0 7 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 4 0.0 3 0 0 0 4 67 13 0.0 3 0 0 0 13 70 3 0.0 3 0 0 0 3 71 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 110 14 0.0 3 0 0 0 14 115 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 4 0.0 3 0 0 0 4 138 16 0.0 3 0 0 0 16 139 1 0.0 3 0 0 1 147 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 3 0.0 3 0 0 0 3 157 1 0.0 3 0 0 0 1 158 2 0.0 3 1 0 0 1 159 296 0.0 3 0 0 2 294 160 26 0.0 3 0 0 0 26 161 3 0.0 3 0 0 0 3 162 40 0.0 3 0 0 0 40 163 6 0.0 3 0 0 0 6 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 0 2 168 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 11 0.0 3 0 0 0 11 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 7 0.0 3 0 0 1 6 177 1 0.0 3 0 0 0 1 178 12 0.0 3 0 0 0 12 179 78 0.0 3 0 0 1 77 180 49 0.0 3 0 0 0 49 181 3 0.0 3 0 0 0 3 182 8 0.0 3 0 0 0 8 183 2 0.0 3 0 0 0 2 185 3 0.0 3 0 0 0 3 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 206 1 0.0 3 0 0 0 1 210 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 246 2 0.0 3 0 0 0 2 256 1 0.0 3 1 258 1 0.0 3 0 0 0 1 262 2 0.0 3 0 0 0 2 271 11 0.0 3 0 0 0 11 272 9 0.0 3 0 0 0 9 273 2 0.0 3 0 0 0 2 274 8 0.0 3 0 0 0 8 275 7 0.0 3 0 0 1 6 276 2 0.0 3 0 0 0 2 277 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 280 6 0.0 3 0 0 0 6 281 12 0.0 3 0 0 0 12 282 3 0.0 3 0 0 0 3 284 2 0.0 3 0 0 0 2 285 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 287 4 0.0 3 0 0 0 4 289 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.38 s (55 us/read; 1.10 M reads/minute). === Summary === Total reads processed: 25,315 Reads with adapters: 113 (0.4%) Reads written (passing filters): 25,315 (100.0%) Total basepairs processed: 6,911,507 bp Total written (filtered): 6,911,158 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 113 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 37.2% C: 15.9% G: 27.4% T: 19.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 107 395.5 0 107 4 4 98.9 0 4 6 2 6.2 0 2