This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 144.61 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 105,731 (3.3%) Reads that were too short: 15,606 (0.5%) Reads written (passing filters): 90,125 (2.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 24,145,086 bp (2.5%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 105731 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1221 773.1 0 1221 7 32548 773.1 0 32548 8 400 773.1 0 400 9 35414 773.1 0 35414 10 85 773.1 0 85 11 87 773.1 0 87 12 36 773.1 0 36 13 44 773.1 0 44 14 40 773.1 0 40 15 46 773.1 0 46 16 36 773.1 0 36 17 79 773.1 0 79 18 32 773.1 0 32 19 41 773.1 0 41 20 49 773.1 0 49 21 42 773.1 0 42 22 60 773.1 0 60 23 46 773.1 0 46 24 46 773.1 0 46 25 53 773.1 0 53 26 34 773.1 0 34 27 31 773.1 0 31 28 34 773.1 0 34 29 81 773.1 0 81 30 56 773.1 0 56 31 65 773.1 0 65 32 43 773.1 0 43 33 55 773.1 0 55 34 37 773.1 0 37 35 40 773.1 0 40 36 46 773.1 0 46 37 63 773.1 0 63 38 49 773.1 0 49 39 51 773.1 0 51 40 61 773.1 0 61 41 46 773.1 0 46 42 55 773.1 0 55 43 38 773.1 0 38 44 40 773.1 0 40 45 29 773.1 0 29 46 53 773.1 0 53 47 57 773.1 0 57 48 31 773.1 0 31 49 61 773.1 0 61 50 41 773.1 0 41 51 27 773.1 0 27 52 62 773.1 0 62 53 67 773.1 0 67 54 50 773.1 0 50 55 64 773.1 0 64 56 49 773.1 0 49 57 34 773.1 0 34 58 55 773.1 0 55 59 41 773.1 0 41 60 48 773.1 0 48 61 41 773.1 0 41 62 54 773.1 0 54 63 51 773.1 0 51 64 51 773.1 0 51 65 76 773.1 0 76 66 46 773.1 0 46 67 68 773.1 0 68 68 69 773.1 0 69 69 64 773.1 0 64 70 104 773.1 0 104 71 697 773.1 0 697 72 379 773.1 0 379 73 477 773.1 0 477 74 126 773.1 0 126 75 168 773.1 0 168 76 62 773.1 0 62 77 36 773.1 0 36 78 70 773.1 0 70 79 74 773.1 0 74 80 60 773.1 0 60 81 75 773.1 0 75 82 89 773.1 0 89 83 51 773.1 0 51 84 47 773.1 0 47 85 58 773.1 0 58 86 56 773.1 0 56 87 68 773.1 0 68 88 52 773.1 0 52 89 57 773.1 0 57 90 63 773.1 0 63 91 56 773.1 0 56 92 48 773.1 0 48 93 47 773.1 0 47 94 39 773.1 0 39 95 40 773.1 0 40 96 61 773.1 0 61 97 47 773.1 0 47 98 44 773.1 0 44 99 107 773.1 0 107 100 100 773.1 0 100 101 852 773.1 0 852 102 419 773.1 0 419 103 545 773.1 0 545 104 84 773.1 0 84 105 59 773.1 0 59 106 74 773.1 0 74 107 42 773.1 0 42 108 63 773.1 0 63 109 66 773.1 0 66 110 75 773.1 0 75 111 227 773.1 0 227 112 219 773.1 0 219 113 320 773.1 0 320 114 152 773.1 0 152 115 237 773.1 0 237 116 81 773.1 0 81 117 113 773.1 0 113 118 54 773.1 0 54 119 68 773.1 0 68 120 55 773.1 0 55 121 62 773.1 0 62 122 67 773.1 0 67 123 122 773.1 0 122 124 133 773.1 0 133 125 96 773.1 0 96 126 89 773.1 0 89 127 62 773.1 0 62 128 65 773.1 0 65 129 60 773.1 0 60 130 106 773.1 0 106 131 99 773.1 0 99 132 540 773.1 0 540 133 185 773.1 0 185 134 177 773.1 0 177 135 68 773.1 0 68 136 103 773.1 0 103 137 193 773.1 0 193 138 195 773.1 0 195 139 212 773.1 0 212 140 92 773.1 0 92 141 112 773.1 0 112 142 131 773.1 0 131 143 124 773.1 0 124 144 304 773.1 0 304 145 219 773.1 0 219 146 325 773.1 0 325 147 74 773.1 0 74 148 85 773.1 0 85 149 159 773.1 0 159 150 147 773.1 0 147 151 134 773.1 0 134 152 72 773.1 0 72 153 55 773.1 0 55 154 145 773.1 0 145 155 115 773.1 0 115 156 166 773.1 0 166 157 83 773.1 0 83 158 113 773.1 0 113 159 97 773.1 0 97 160 87 773.1 0 87 161 69 773.1 0 69 162 83 773.1 0 83 163 92 773.1 0 92 164 130 773.1 0 130 165 92 773.1 0 92 166 109 773.1 0 109 167 51 773.1 0 51 168 87 773.1 0 87 169 131 773.1 0 131 170 433 773.1 0 433 171 302 773.1 0 302 172 313 773.1 0 313 173 57 773.1 0 57 174 43 773.1 0 43 175 54 773.1 0 54 176 66 773.1 0 66 177 123 773.1 0 123 178 321 773.1 0 321 179 309 773.1 0 309 180 264 773.1 0 264 181 59 773.1 0 59 182 58 773.1 0 58 183 83 773.1 0 83 184 71 773.1 0 71 185 83 773.1 0 83 186 81 773.1 0 81 187 81 773.1 0 81 188 93 773.1 0 93 189 159 773.1 0 159 190 64 773.1 0 64 191 54 773.1 0 54 192 48 773.1 0 48 193 39 773.1 0 39 194 61 773.1 0 61 195 52 773.1 0 52 196 69 773.1 0 69 197 95 773.1 0 95 198 97 773.1 0 97 199 124 773.1 0 124 200 96 773.1 0 96 201 74 773.1 0 74 202 115 773.1 0 115 203 99 773.1 0 99 204 203 773.1 0 203 205 173 773.1 0 173 206 126 773.1 0 126 207 69 773.1 0 69 208 50 773.1 0 50 209 76 773.1 0 76 210 130 773.1 0 130 211 202 773.1 0 202 212 115 773.1 0 115 213 85 773.1 0 85 214 96 773.1 0 96 215 81 773.1 0 81 216 73 773.1 0 73 217 67 773.1 0 67 218 80 773.1 0 80 219 83 773.1 0 83 220 71 773.1 0 71 221 66 773.1 0 66 222 61 773.1 0 61 223 111 773.1 0 111 224 159 773.1 0 159 225 181 773.1 0 181 226 245 773.1 0 245 227 209 773.1 0 209 228 167 773.1 0 167 229 106 773.1 0 106 230 61 773.1 0 61 231 71 773.1 0 71 232 69 773.1 0 69 233 82 773.1 0 82 234 68 773.1 0 68 235 79 773.1 0 79 236 106 773.1 0 106 237 93 773.1 0 93 238 91 773.1 0 91 239 77 773.1 0 77 240 74 773.1 0 74 241 73 773.1 0 73 242 67 773.1 0 67 243 113 773.1 0 113 244 172 773.1 0 172 245 232 773.1 0 232 246 197 773.1 0 197 247 286 773.1 0 286 248 191 773.1 0 191 249 101 773.1 0 101 250 80 773.1 0 80 251 59 773.1 0 59 252 72 773.1 0 72 253 69 773.1 0 69 254 76 773.1 0 76 255 82 773.1 0 82 256 126 773.1 0 126 257 177 773.1 0 177 258 168 773.1 0 168 259 198 773.1 0 198 260 149 773.1 0 149 261 149 773.1 0 149 262 121 773.1 0 121 263 104 773.1 0 104 264 273 773.1 0 273 265 247 773.1 0 247 266 331 773.1 0 331 267 96 773.1 0 96 268 110 773.1 0 110 269 76 773.1 0 76 270 104 773.1 0 104 271 167 773.1 0 167 272 265 773.1 0 265 273 218 773.1 0 218 274 187 773.1 0 187 275 104 773.1 0 104 276 81 773.1 0 81 277 103 773.1 0 103 278 83 773.1 0 83 279 122 773.1 0 122 280 125 773.1 0 125 281 133 773.1 0 133 282 164 773.1 0 164 283 215 773.1 0 215 284 758 773.1 0 758 285 986 773.1 0 986 286 1159 773.1 0 1159 287 694 773.1 0 694 288 145 773.1 0 145 289 92 773.1 0 92 290 70 773.1 0 70 291 133 773.1 0 133 292 162 773.1 0 162 293 206 773.1 0 206 294 83 773.1 0 83 295 85 773.1 0 85 296 76 773.1 0 76 297 73 773.1 0 73 298 94 773.1 0 94 299 105 773.1 0 105 300 104 773.1 0 104 301 145 773.1 0 145 Finished in 6.72 s (75 us/read; 0.80 M reads/minute). === Summary === Total reads processed: 90,125 Reads with adapters: 33,302 (37.0%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 33,280 (36.9%) Total basepairs processed: 24,145,086 bp Total written (filtered): 9,104,510 bp (37.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 33302 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 4 0.0 3 0 0 2 1 1 19 170 0.0 3 0 125 29 8 8 20 32333 0.0 4 30862 1221 176 63 11 21 73 0.0 4 10 54 4 2 3 22 4 0.0 4 0 0 2 1 1 23 564 0.0 4 483 22 5 41 13 24 3 0.0 4 0 1 0 1 1 26 4 0.0 4 0 0 0 3 1 35 1 0.0 4 1 42 1 0.0 4 1 74 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 4 0.0 4 0 0 0 0 4 126 3 0.0 4 0 0 0 0 3 127 112 0.0 4 0 0 0 0 112 153 2 0.0 4 0 0 0 0 2 170 2 0.0 4 0 0 0 0 2 190 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 1 228 3 0.0 4 0 0 0 0 3 229 2 0.0 4 0 0 0 0 2 233 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.85 s (56 us/read; 1.08 M reads/minute). === Summary === Total reads processed: 33,280 Reads with adapters: 1,933 (5.8%) Reads written (passing filters): 33,280 (100.0%) Total basepairs processed: 9,104,510 bp Total written (filtered): 9,081,546 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1933 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.0% C: 8.4% G: 84.8% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 108 520.0 0 108 4 19 130.0 0 19 5 3 32.5 0 3 9 76 0.1 0 6 70 10 83 0.0 1 2 81 11 1477 0.0 1 32 1445 12 122 0.0 1 3 119 13 21 0.0 1 0 21 14 2 0.0 1 0 2 19 6 0.0 1 5 1 52 1 0.0 1 1 73 1 0.0 1 0 1 152 2 0.0 1 1 1 191 2 0.0 1 0 2 208 1 0.0 1 1 210 9 0.0 1 5 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.