This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 144.90 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 94,643 (3.0%) Reads that were too short: 9,073 (0.3%) Reads written (passing filters): 85,570 (2.7%) Total basepairs processed: 953,096,634 bp Total written (filtered): 23,636,319 bp (2.5%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 94643 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1326 773.1 0 1326 7 38429 773.1 0 38429 8 284 773.1 0 284 9 31721 773.1 0 31721 10 61 773.1 0 61 11 59 773.1 0 59 12 29 773.1 0 29 13 25 773.1 0 25 14 26 773.1 0 26 15 33 773.1 0 33 16 19 773.1 0 19 17 23 773.1 0 23 18 25 773.1 0 25 19 28 773.1 0 28 20 38 773.1 0 38 21 43 773.1 0 43 22 25 773.1 0 25 23 32 773.1 0 32 24 41 773.1 0 41 25 27 773.1 0 27 26 27 773.1 0 27 27 31 773.1 0 31 28 28 773.1 0 28 29 29 773.1 0 29 30 24 773.1 0 24 31 30 773.1 0 30 32 36 773.1 0 36 33 30 773.1 0 30 34 22 773.1 0 22 35 21 773.1 0 21 36 25 773.1 0 25 37 26 773.1 0 26 38 26 773.1 0 26 39 24 773.1 0 24 40 26 773.1 0 26 41 46 773.1 0 46 42 35 773.1 0 35 43 42 773.1 0 42 44 31 773.1 0 31 45 31 773.1 0 31 46 33 773.1 0 33 47 39 773.1 0 39 48 25 773.1 0 25 49 33 773.1 0 33 50 118 773.1 0 118 51 23 773.1 0 23 52 31 773.1 0 31 53 42 773.1 0 42 54 36 773.1 0 36 55 47 773.1 0 47 56 29 773.1 0 29 57 34 773.1 0 34 58 35 773.1 0 35 59 30 773.1 0 30 60 30 773.1 0 30 61 25 773.1 0 25 62 29 773.1 0 29 63 28 773.1 0 28 64 41 773.1 0 41 65 31 773.1 0 31 66 30 773.1 0 30 67 43 773.1 0 43 68 62 773.1 0 62 69 46 773.1 0 46 70 103 773.1 0 103 71 921 773.1 0 921 72 558 773.1 0 558 73 625 773.1 0 625 74 108 773.1 0 108 75 160 773.1 0 160 76 30 773.1 0 30 77 23 773.1 0 23 78 37 773.1 0 37 79 36 773.1 0 36 80 61 773.1 0 61 81 65 773.1 0 65 82 81 773.1 0 81 83 31 773.1 0 31 84 33 773.1 0 33 85 28 773.1 0 28 86 36 773.1 0 36 87 28 773.1 0 28 88 35 773.1 0 35 89 34 773.1 0 34 90 41 773.1 0 41 91 43 773.1 0 43 92 41 773.1 0 41 93 44 773.1 0 44 94 29 773.1 0 29 95 44 773.1 0 44 96 29 773.1 0 29 97 30 773.1 0 30 98 41 773.1 0 41 99 50 773.1 0 50 100 45 773.1 0 45 101 389 773.1 0 389 102 252 773.1 0 252 103 211 773.1 0 211 104 33 773.1 0 33 105 31 773.1 0 31 106 35 773.1 0 35 107 26 773.1 0 26 108 25 773.1 0 25 109 30 773.1 0 30 110 34 773.1 0 34 111 125 773.1 0 125 112 119 773.1 0 119 113 198 773.1 0 198 114 92 773.1 0 92 115 174 773.1 0 174 116 39 773.1 0 39 117 53 773.1 0 53 118 33 773.1 0 33 119 23 773.1 0 23 120 26 773.1 0 26 121 37 773.1 0 37 122 36 773.1 0 36 123 51 773.1 0 51 124 101 773.1 0 101 125 58 773.1 0 58 126 34 773.1 0 34 127 22 773.1 0 22 128 24 773.1 0 24 129 32 773.1 0 32 130 51 773.1 0 51 131 71 773.1 0 71 132 442 773.1 0 442 133 183 773.1 0 183 134 160 773.1 0 160 135 44 773.1 0 44 136 47 773.1 0 47 137 120 773.1 0 120 138 98 773.1 0 98 139 120 773.1 0 120 140 39 773.1 0 39 141 73 773.1 0 73 142 60 773.1 0 60 143 56 773.1 0 56 144 252 773.1 0 252 145 140 773.1 0 140 146 278 773.1 0 278 147 48 773.1 0 48 148 56 773.1 0 56 149 143 773.1 0 143 150 117 773.1 0 117 151 85 773.1 0 85 152 40 773.1 0 40 153 48 773.1 0 48 154 105 773.1 0 105 155 72 773.1 0 72 156 114 773.1 0 114 157 48 773.1 0 48 158 72 773.1 0 72 159 46 773.1 0 46 160 44 773.1 0 44 161 46 773.1 0 46 162 38 773.1 0 38 163 54 773.1 0 54 164 63 773.1 0 63 165 41 773.1 0 41 166 51 773.1 0 51 167 29 773.1 0 29 168 46 773.1 0 46 169 71 773.1 0 71 170 244 773.1 0 244 171 201 773.1 0 201 172 229 773.1 0 229 173 23 773.1 0 23 174 30 773.1 0 30 175 35 773.1 0 35 176 36 773.1 0 36 177 59 773.1 0 59 178 137 773.1 0 137 179 137 773.1 0 137 180 103 773.1 0 103 181 28 773.1 0 28 182 34 773.1 0 34 183 33 773.1 0 33 184 44 773.1 0 44 185 49 773.1 0 49 186 45 773.1 0 45 187 66 773.1 0 66 188 49 773.1 0 49 189 162 773.1 0 162 190 40 773.1 0 40 191 30 773.1 0 30 192 27 773.1 0 27 193 32 773.1 0 32 194 28 773.1 0 28 195 34 773.1 0 34 196 48 773.1 0 48 197 54 773.1 0 54 198 54 773.1 0 54 199 48 773.1 0 48 200 56 773.1 0 56 201 47 773.1 0 47 202 53 773.1 0 53 203 55 773.1 0 55 204 67 773.1 0 67 205 68 773.1 0 68 206 46 773.1 0 46 207 35 773.1 0 35 208 29 773.1 0 29 209 36 773.1 0 36 210 86 773.1 0 86 211 150 773.1 0 150 212 81 773.1 0 81 213 48 773.1 0 48 214 55 773.1 0 55 215 43 773.1 0 43 216 30 773.1 0 30 217 51 773.1 0 51 218 65 773.1 0 65 219 47 773.1 0 47 220 43 773.1 0 43 221 34 773.1 0 34 222 51 773.1 0 51 223 62 773.1 0 62 224 111 773.1 0 111 225 93 773.1 0 93 226 131 773.1 0 131 227 121 773.1 0 121 228 98 773.1 0 98 229 87 773.1 0 87 230 33 773.1 0 33 231 35 773.1 0 35 232 34 773.1 0 34 233 45 773.1 0 45 234 41 773.1 0 41 235 49 773.1 0 49 236 64 773.1 0 64 237 55 773.1 0 55 238 49 773.1 0 49 239 38 773.1 0 38 240 39 773.1 0 39 241 44 773.1 0 44 242 47 773.1 0 47 243 55 773.1 0 55 244 81 773.1 0 81 245 107 773.1 0 107 246 112 773.1 0 112 247 199 773.1 0 199 248 102 773.1 0 102 249 73 773.1 0 73 250 36 773.1 0 36 251 36 773.1 0 36 252 40 773.1 0 40 253 51 773.1 0 51 254 45 773.1 0 45 255 67 773.1 0 67 256 72 773.1 0 72 257 91 773.1 0 91 258 99 773.1 0 99 259 139 773.1 0 139 260 87 773.1 0 87 261 103 773.1 0 103 262 89 773.1 0 89 263 76 773.1 0 76 264 130 773.1 0 130 265 159 773.1 0 159 266 172 773.1 0 172 267 54 773.1 0 54 268 66 773.1 0 66 269 45 773.1 0 45 270 49 773.1 0 49 271 100 773.1 0 100 272 143 773.1 0 143 273 121 773.1 0 121 274 77 773.1 0 77 275 57 773.1 0 57 276 63 773.1 0 63 277 89 773.1 0 89 278 47 773.1 0 47 279 83 773.1 0 83 280 72 773.1 0 72 281 78 773.1 0 78 282 81 773.1 0 81 283 98 773.1 0 98 284 405 773.1 0 405 285 631 773.1 0 631 286 783 773.1 0 783 287 299 773.1 0 299 288 88 773.1 0 88 289 46 773.1 0 46 290 37 773.1 0 37 291 70 773.1 0 70 292 105 773.1 0 105 293 78 773.1 0 78 294 51 773.1 0 51 295 60 773.1 0 60 296 48 773.1 0 48 297 50 773.1 0 50 298 75 773.1 0 75 299 62 773.1 0 62 300 86 773.1 0 86 301 106 773.1 0 106 Finished in 6.00 s (70 us/read; 0.86 M reads/minute). === Summary === Total reads processed: 85,570 Reads with adapters: 32,291 (37.7%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 32,273 (37.7%) Total basepairs processed: 23,636,319 bp Total written (filtered): 8,872,593 bp (37.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 32291 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 85 0.0 3 0 83 1 1 17 32034 0.0 3 31403 615 15 1 18 62 0.0 3 6 53 3 19 15 0.0 3 0 0 14 1 20 51 0.0 3 0 0 0 51 21 1 0.0 3 0 0 0 1 24 1 0.0 3 1 45 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 1 1 50 2 0.0 3 0 0 1 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 165 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 194 5 0.0 3 0 0 0 5 208 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 280 3 0.0 3 0 0 0 3 281 3 0.0 3 0 0 0 3 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.78 s (55 us/read; 1.09 M reads/minute). === Summary === Total reads processed: 32,273 Reads with adapters: 1,407 (4.4%) Reads written (passing filters): 32,273 (100.0%) Total basepairs processed: 8,872,593 bp Total written (filtered): 8,868,357 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1407 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 43.8% C: 2.9% G: 52.5% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1393 504.3 0 1393 4 13 126.1 0 13 5 1 31.5 0 1