This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 142.55 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 216,444 (6.8%) Reads that were too short: 131,777 (4.2%) Reads written (passing filters): 84,667 (2.7%) Total basepairs processed: 953,096,634 bp Total written (filtered): 22,231,784 bp (2.3%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 216444 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 489 773.1 0 489 7 1010 773.1 0 1010 8 31257 773.1 0 31257 9 28942 773.1 0 28942 10 46 773.1 0 46 11 44 773.1 0 44 12 28 773.1 0 28 13 22 773.1 0 22 14 21 773.1 0 21 15 33 773.1 0 33 16 10 773.1 0 10 17 15 773.1 0 15 18 11 773.1 0 11 19 15 773.1 0 15 20 16 773.1 0 16 21 17 773.1 0 17 22 19 773.1 0 19 23 23 773.1 0 23 24 19 773.1 0 19 25 17 773.1 0 17 26 34 773.1 0 34 27 25 773.1 0 25 28 32 773.1 0 32 29 18 773.1 0 18 30 19 773.1 0 19 31 13 773.1 0 13 32 17 773.1 0 17 33 30 773.1 0 30 34 24 773.1 0 24 35 19 773.1 0 19 36 26 773.1 0 26 37 26 773.1 0 26 38 26 773.1 0 26 39 23 773.1 0 23 40 16 773.1 0 16 41 19 773.1 0 19 42 24 773.1 0 24 43 37 773.1 0 37 44 36 773.1 0 36 45 45 773.1 0 45 46 31 773.1 0 31 47 18 773.1 0 18 48 31 773.1 0 31 49 38 773.1 0 38 50 38 773.1 0 38 51 36 773.1 0 36 52 20 773.1 0 20 53 20 773.1 0 20 54 33 773.1 0 33 55 31 773.1 0 31 56 139 773.1 0 139 57 153 773.1 0 153 58 107 773.1 0 107 59 27 773.1 0 27 60 30 773.1 0 30 61 62 773.1 0 62 62 38 773.1 0 38 63 37 773.1 0 37 64 47 773.1 0 47 65 33 773.1 0 33 66 15 773.1 0 15 67 40 773.1 0 40 68 51 773.1 0 51 69 143 773.1 0 143 70 168 773.1 0 168 71 327 773.1 0 327 72 51 773.1 0 51 73 49 773.1 0 49 74 49 773.1 0 49 75 52 773.1 0 52 76 68 773.1 0 68 77 43 773.1 0 43 78 61 773.1 0 61 79 54 773.1 0 54 80 52 773.1 0 52 81 45 773.1 0 45 82 48 773.1 0 48 83 42 773.1 0 42 84 42 773.1 0 42 85 51 773.1 0 51 86 56 773.1 0 56 87 103 773.1 0 103 88 111 773.1 0 111 89 2398 773.1 0 2398 90 2049 773.1 0 2049 91 2655 773.1 0 2655 92 46 773.1 0 46 93 39 773.1 0 39 94 36 773.1 0 36 95 34 773.1 0 34 96 37 773.1 0 37 97 174 773.1 0 174 98 126 773.1 0 126 99 95 773.1 0 95 100 82 773.1 0 82 101 36 773.1 0 36 102 51 773.1 0 51 103 35 773.1 0 35 104 41 773.1 0 41 105 34 773.1 0 34 106 36 773.1 0 36 107 33 773.1 0 33 108 53 773.1 0 53 109 27 773.1 0 27 110 146 773.1 0 146 111 77 773.1 0 77 112 100 773.1 0 100 113 27 773.1 0 27 114 19 773.1 0 19 115 12 773.1 0 12 116 26 773.1 0 26 117 31 773.1 0 31 118 74 773.1 0 74 119 28 773.1 0 28 120 26 773.1 0 26 121 33 773.1 0 33 122 31 773.1 0 31 123 23 773.1 0 23 124 19 773.1 0 19 125 24 773.1 0 24 126 23 773.1 0 23 127 54 773.1 0 54 128 75 773.1 0 75 129 29 773.1 0 29 130 28 773.1 0 28 131 31 773.1 0 31 132 63 773.1 0 63 133 50 773.1 0 50 134 116 773.1 0 116 135 72 773.1 0 72 136 88 773.1 0 88 137 1326 773.1 0 1326 138 543 773.1 0 543 139 991 773.1 0 991 140 33 773.1 0 33 141 25 773.1 0 25 142 34 773.1 0 34 143 26 773.1 0 26 144 33 773.1 0 33 145 31 773.1 0 31 146 75 773.1 0 75 147 43 773.1 0 43 148 122 773.1 0 122 149 149 773.1 0 149 150 183 773.1 0 183 151 33 773.1 0 33 152 34 773.1 0 34 153 31 773.1 0 31 154 32 773.1 0 32 155 48 773.1 0 48 156 49 773.1 0 49 157 63 773.1 0 63 158 147 773.1 0 147 159 65 773.1 0 65 160 64 773.1 0 64 161 67 773.1 0 67 162 72 773.1 0 72 163 23 773.1 0 23 164 36 773.1 0 36 165 43 773.1 0 43 166 40 773.1 0 40 167 35 773.1 0 35 168 383 773.1 0 383 169 256 773.1 0 256 170 136 773.1 0 136 171 20 773.1 0 20 172 25 773.1 0 25 173 32 773.1 0 32 174 78 773.1 0 78 175 116 773.1 0 116 176 1389 773.1 0 1389 177 951 773.1 0 951 178 1124 773.1 0 1124 179 76 773.1 0 76 180 43 773.1 0 43 181 17 773.1 0 17 182 72 773.1 0 72 183 58 773.1 0 58 184 45 773.1 0 45 185 39 773.1 0 39 186 37 773.1 0 37 187 39 773.1 0 39 188 31 773.1 0 31 189 69 773.1 0 69 190 36 773.1 0 36 191 35 773.1 0 35 192 32 773.1 0 32 193 31 773.1 0 31 194 41 773.1 0 41 195 37 773.1 0 37 196 53 773.1 0 53 197 43 773.1 0 43 198 35 773.1 0 35 199 22 773.1 0 22 200 28 773.1 0 28 201 47 773.1 0 47 202 122 773.1 0 122 203 114 773.1 0 114 204 95 773.1 0 95 205 72 773.1 0 72 206 65 773.1 0 65 207 106 773.1 0 106 208 140 773.1 0 140 209 139 773.1 0 139 210 169 773.1 0 169 211 110 773.1 0 110 212 134 773.1 0 134 213 70 773.1 0 70 214 43 773.1 0 43 215 75 773.1 0 75 216 77 773.1 0 77 217 93 773.1 0 93 218 115 773.1 0 115 219 353 773.1 0 353 220 2177 773.1 0 2177 221 1450 773.1 0 1450 222 2353 773.1 0 2353 223 289 773.1 0 289 224 49 773.1 0 49 225 98 773.1 0 98 226 48 773.1 0 48 227 56 773.1 0 56 228 29 773.1 0 29 229 34 773.1 0 34 230 100 773.1 0 100 231 130 773.1 0 130 232 171 773.1 0 171 233 112 773.1 0 112 234 71 773.1 0 71 235 72 773.1 0 72 236 58 773.1 0 58 237 46 773.1 0 46 238 39 773.1 0 39 239 118 773.1 0 118 240 157 773.1 0 157 241 165 773.1 0 165 242 142 773.1 0 142 243 116 773.1 0 116 244 164 773.1 0 164 245 132 773.1 0 132 246 66 773.1 0 66 247 66 773.1 0 66 248 51 773.1 0 51 249 102 773.1 0 102 250 110 773.1 0 110 251 122 773.1 0 122 252 52 773.1 0 52 253 101 773.1 0 101 254 94 773.1 0 94 255 202 773.1 0 202 256 135 773.1 0 135 257 138 773.1 0 138 258 136 773.1 0 136 259 88 773.1 0 88 260 100 773.1 0 100 261 128 773.1 0 128 262 285 773.1 0 285 263 391 773.1 0 391 264 411 773.1 0 411 265 322 773.1 0 322 266 192 773.1 0 192 267 148 773.1 0 148 268 407 773.1 0 407 269 978 773.1 0 978 270 1831 773.1 0 1831 271 986 773.1 0 986 272 460 773.1 0 460 273 192 773.1 0 192 274 3463 773.1 0 3463 275 3306 773.1 0 3306 276 1705 773.1 0 1705 277 168 773.1 0 168 278 276 773.1 0 276 279 362 773.1 0 362 280 586 773.1 0 586 281 1292 773.1 0 1292 282 7351 773.1 0 7351 283 33134 773.1 0 33134 284 28227 773.1 0 28227 285 25244 773.1 0 25244 286 4847 773.1 0 4847 287 120 773.1 0 120 288 628 773.1 0 628 289 565 773.1 0 565 290 867 773.1 0 867 291 325 773.1 0 325 292 50 773.1 0 50 293 36 773.1 0 36 294 38 773.1 0 38 295 48 773.1 0 48 296 69 773.1 0 69 297 91 773.1 0 91 298 78 773.1 0 78 299 61 773.1 0 61 300 41 773.1 0 41 301 37 773.1 0 37 Finished in 6.08 s (72 us/read; 0.84 M reads/minute). === Summary === Total reads processed: 84,667 Reads with adapters: 29,839 (35.2%) Reads that were too short: 43 (0.1%) Reads written (passing filters): 29,796 (35.2%) Total basepairs processed: 22,231,784 bp Total written (filtered): 8,100,888 bp (36.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 29839 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 11 0.0 3 0 0 6 1 4 19 126 0.0 3 0 92 13 11 10 20 29444 0.0 4 27894 1239 228 61 22 21 57 0.0 4 8 41 7 0 1 22 22 0.0 4 7 2 8 2 3 23 89 0.0 4 1 1 0 57 30 24 2 0.0 4 1 0 0 0 1 27 1 0.0 4 0 0 0 1 30 1 0.0 4 1 32 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 44 2 0.0 4 0 0 0 0 2 48 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 71 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 31 0.0 4 0 0 0 0 31 131 1 0.0 4 0 0 0 0 1 135 2 0.0 4 0 0 0 0 2 146 7 0.0 4 0 0 0 0 7 147 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 1 168 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 209 1 0.0 4 0 0 0 0 1 213 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 0 2 229 5 0.0 4 0 0 0 0 5 232 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 2 0.0 4 0 0 0 0 2 284 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.78 s (60 us/read; 1.00 M reads/minute). === Summary === Total reads processed: 29,796 Reads with adapters: 8,622 (28.9%) Reads written (passing filters): 29,796 (100.0%) Total basepairs processed: 8,100,888 bp Total written (filtered): 8,012,623 bp (98.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8622 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.3% C: 5.5% G: 91.8% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 465.6 0 6 4 5 116.4 0 5 6 8 7.3 0 8 7 1 1.8 0 1 8 4 0.5 0 4 9 4330 0.1 0 279 4051 10 3765 0.0 1 260 3505 11 444 0.0 1 9 435 12 18 0.0 1 0 18 13 4 0.0 1 0 4 16 1 0.0 1 0 1 72 2 0.0 1 2 150 19 0.0 1 10 9 208 5 0.0 1 4 1 223 8 0.0 1 8 267 2 0.0 1 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.