This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 146.58 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 188,364 (5.9%) Reads that were too short: 105,563 (3.3%) Reads written (passing filters): 82,801 (2.6%) Total basepairs processed: 953,096,634 bp Total written (filtered): 21,971,562 bp (2.3%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 188364 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 450 773.1 0 450 7 802 773.1 0 802 8 29652 773.1 0 29652 9 32602 773.1 0 32602 10 47 773.1 0 47 11 21 773.1 0 21 12 5 773.1 0 5 13 4 773.1 0 4 14 7 773.1 0 7 15 9 773.1 0 9 16 4 773.1 0 4 17 5 773.1 0 5 18 6 773.1 0 6 19 7 773.1 0 7 20 7 773.1 0 7 21 53 773.1 0 53 22 14 773.1 0 14 23 8 773.1 0 8 24 9 773.1 0 9 25 11 773.1 0 11 26 7 773.1 0 7 27 7 773.1 0 7 28 19 773.1 0 19 29 7 773.1 0 7 30 6 773.1 0 6 31 4 773.1 0 4 32 12 773.1 0 12 33 8 773.1 0 8 34 9 773.1 0 9 35 3 773.1 0 3 36 12 773.1 0 12 37 13 773.1 0 13 38 23 773.1 0 23 39 8 773.1 0 8 40 2 773.1 0 2 41 9 773.1 0 9 42 11 773.1 0 11 43 23 773.1 0 23 44 40 773.1 0 40 45 33 773.1 0 33 46 19 773.1 0 19 47 9 773.1 0 9 48 15 773.1 0 15 49 27 773.1 0 27 50 23 773.1 0 23 51 25 773.1 0 25 52 14 773.1 0 14 53 11 773.1 0 11 54 5 773.1 0 5 55 17 773.1 0 17 56 527 773.1 0 527 57 135 773.1 0 135 58 44 773.1 0 44 59 15 773.1 0 15 60 16 773.1 0 16 61 31 773.1 0 31 62 33 773.1 0 33 63 17 773.1 0 17 64 21 773.1 0 21 65 25 773.1 0 25 66 11 773.1 0 11 67 21 773.1 0 21 68 27 773.1 0 27 69 99 773.1 0 99 70 76 773.1 0 76 71 134 773.1 0 134 72 25 773.1 0 25 73 26 773.1 0 26 74 25 773.1 0 25 75 37 773.1 0 37 76 52 773.1 0 52 77 25 773.1 0 25 78 27 773.1 0 27 79 35 773.1 0 35 80 36 773.1 0 36 81 32 773.1 0 32 82 27 773.1 0 27 83 23 773.1 0 23 84 39 773.1 0 39 85 43 773.1 0 43 86 41 773.1 0 41 87 62 773.1 0 62 88 81 773.1 0 81 89 2162 773.1 0 2162 90 1542 773.1 0 1542 91 2025 773.1 0 2025 92 21 773.1 0 21 93 13 773.1 0 13 94 9 773.1 0 9 95 14 773.1 0 14 96 13 773.1 0 13 97 45 773.1 0 45 98 42 773.1 0 42 99 28 773.1 0 28 100 44 773.1 0 44 101 25 773.1 0 25 102 24 773.1 0 24 103 17 773.1 0 17 104 16 773.1 0 16 105 22 773.1 0 22 106 18 773.1 0 18 107 22 773.1 0 22 108 15 773.1 0 15 109 18 773.1 0 18 110 99 773.1 0 99 111 34 773.1 0 34 112 74 773.1 0 74 113 19 773.1 0 19 114 16 773.1 0 16 115 5 773.1 0 5 116 13 773.1 0 13 117 16 773.1 0 16 118 53 773.1 0 53 119 12 773.1 0 12 120 25 773.1 0 25 121 9 773.1 0 9 122 18 773.1 0 18 123 8 773.1 0 8 124 15 773.1 0 15 125 19 773.1 0 19 126 14 773.1 0 14 127 25 773.1 0 25 128 31 773.1 0 31 129 13 773.1 0 13 130 18 773.1 0 18 131 10 773.1 0 10 132 40 773.1 0 40 133 26 773.1 0 26 134 74 773.1 0 74 135 46 773.1 0 46 136 76 773.1 0 76 137 1318 773.1 0 1318 138 548 773.1 0 548 139 1001 773.1 0 1001 140 18 773.1 0 18 141 20 773.1 0 20 142 16 773.1 0 16 143 10 773.1 0 10 144 13 773.1 0 13 145 21 773.1 0 21 146 18 773.1 0 18 147 23 773.1 0 23 148 64 773.1 0 64 149 52 773.1 0 52 150 69 773.1 0 69 151 16 773.1 0 16 152 16 773.1 0 16 153 19 773.1 0 19 154 13 773.1 0 13 155 28 773.1 0 28 156 25 773.1 0 25 157 34 773.1 0 34 158 100 773.1 0 100 159 25 773.1 0 25 160 37 773.1 0 37 161 29 773.1 0 29 162 29 773.1 0 29 163 28 773.1 0 28 164 20 773.1 0 20 165 18 773.1 0 18 166 32 773.1 0 32 167 24 773.1 0 24 168 530 773.1 0 530 169 329 773.1 0 329 170 163 773.1 0 163 171 19 773.1 0 19 172 31 773.1 0 31 173 40 773.1 0 40 174 70 773.1 0 70 175 101 773.1 0 101 176 1894 773.1 0 1894 177 1044 773.1 0 1044 178 1456 773.1 0 1456 179 38 773.1 0 38 180 19 773.1 0 19 181 16 773.1 0 16 182 24 773.1 0 24 183 22 773.1 0 22 184 23 773.1 0 23 185 10 773.1 0 10 186 15 773.1 0 15 187 24 773.1 0 24 188 11 773.1 0 11 189 40 773.1 0 40 190 29 773.1 0 29 191 16 773.1 0 16 192 23 773.1 0 23 193 17 773.1 0 17 194 29 773.1 0 29 195 17 773.1 0 17 196 26 773.1 0 26 197 13 773.1 0 13 198 20 773.1 0 20 199 14 773.1 0 14 200 17 773.1 0 17 201 22 773.1 0 22 202 48 773.1 0 48 203 56 773.1 0 56 204 40 773.1 0 40 205 47 773.1 0 47 206 38 773.1 0 38 207 61 773.1 0 61 208 92 773.1 0 92 209 94 773.1 0 94 210 77 773.1 0 77 211 43 773.1 0 43 212 59 773.1 0 59 213 29 773.1 0 29 214 41 773.1 0 41 215 41 773.1 0 41 216 65 773.1 0 65 217 84 773.1 0 84 218 87 773.1 0 87 219 269 773.1 0 269 220 2524 773.1 0 2524 221 1388 773.1 0 1388 222 2464 773.1 0 2464 223 225 773.1 0 225 224 31 773.1 0 31 225 60 773.1 0 60 226 28 773.1 0 28 227 34 773.1 0 34 228 26 773.1 0 26 229 20 773.1 0 20 230 59 773.1 0 59 231 63 773.1 0 63 232 80 773.1 0 80 233 80 773.1 0 80 234 42 773.1 0 42 235 33 773.1 0 33 236 26 773.1 0 26 237 38 773.1 0 38 238 22 773.1 0 22 239 81 773.1 0 81 240 75 773.1 0 75 241 71 773.1 0 71 242 89 773.1 0 89 243 56 773.1 0 56 244 94 773.1 0 94 245 103 773.1 0 103 246 61 773.1 0 61 247 58 773.1 0 58 248 34 773.1 0 34 249 46 773.1 0 46 250 68 773.1 0 68 251 78 773.1 0 78 252 37 773.1 0 37 253 73 773.1 0 73 254 81 773.1 0 81 255 131 773.1 0 131 256 78 773.1 0 78 257 98 773.1 0 98 258 86 773.1 0 86 259 80 773.1 0 80 260 67 773.1 0 67 261 85 773.1 0 85 262 141 773.1 0 141 263 156 773.1 0 156 264 156 773.1 0 156 265 159 773.1 0 159 266 149 773.1 0 149 267 118 773.1 0 118 268 222 773.1 0 222 269 512 773.1 0 512 270 796 773.1 0 796 271 548 773.1 0 548 272 250 773.1 0 250 273 189 773.1 0 189 274 4574 773.1 0 4574 275 4557 773.1 0 4557 276 2259 773.1 0 2259 277 118 773.1 0 118 278 191 773.1 0 191 279 252 773.1 0 252 280 408 773.1 0 408 281 1128 773.1 0 1128 282 4979 773.1 0 4979 283 29883 773.1 0 29883 284 19208 773.1 0 19208 285 19687 773.1 0 19687 286 3085 773.1 0 3085 287 77 773.1 0 77 288 371 773.1 0 371 289 252 773.1 0 252 290 362 773.1 0 362 291 173 773.1 0 173 292 26 773.1 0 26 293 29 773.1 0 29 294 29 773.1 0 29 295 37 773.1 0 37 296 42 773.1 0 42 297 55 773.1 0 55 298 47 773.1 0 47 299 43 773.1 0 43 300 34 773.1 0 34 301 17 773.1 0 17 Finished in 5.84 s (71 us/read; 0.85 M reads/minute). === Summary === Total reads processed: 82,801 Reads with adapters: 30,147 (36.4%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 30,131 (36.4%) Total basepairs processed: 21,971,562 bp Total written (filtered): 8,300,156 bp (37.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 30147 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 0 1 16 109 0.0 3 0 107 2 17 29831 0.0 3 29323 489 18 1 18 65 0.0 3 4 59 2 19 11 0.0 3 0 0 10 1 20 12 0.0 3 0 0 0 12 23 1 0.0 3 1 32 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 1 1 100 1 0.0 3 0 0 0 1 101 68 0.0 3 0 0 1 67 102 17 0.0 3 0 0 0 17 104 2 0.0 3 0 0 0 2 105 5 0.0 3 0 0 0 5 108 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.62 s (54 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 30,131 Reads with adapters: 210 (0.7%) Reads written (passing filters): 30,131 (100.0%) Total basepairs processed: 8,300,156 bp Total written (filtered): 8,299,419 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 210 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 69.0% C: 5.7% G: 22.4% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 140 470.8 0 140 4 63 117.7 0 63 5 2 29.4 0 2 11 5 0.0 1 0 5