This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 150.77 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 273,363 (8.6%) Reads that were too short: 27,198 (0.9%) Reads written (passing filters): 246,165 (7.8%) Total basepairs processed: 953,096,634 bp Total written (filtered): 61,239,355 bp (6.4%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 273363 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2251 773.1 0 2251 7 78040 773.1 0 78040 8 63307 773.1 0 63307 9 104 773.1 0 104 10 56 773.1 0 56 11 74 773.1 0 74 12 53 773.1 0 53 13 61 773.1 0 61 14 61 773.1 0 61 15 46 773.1 0 46 16 53 773.1 0 53 17 55 773.1 0 55 18 103 773.1 0 103 19 88 773.1 0 88 20 107 773.1 0 107 21 59 773.1 0 59 22 58 773.1 0 58 23 70 773.1 0 70 24 49 773.1 0 49 25 42 773.1 0 42 26 47 773.1 0 47 27 48 773.1 0 48 28 54 773.1 0 54 29 165 773.1 0 165 30 47 773.1 0 47 31 44 773.1 0 44 32 53 773.1 0 53 33 68 773.1 0 68 34 69 773.1 0 69 35 115 773.1 0 115 36 63 773.1 0 63 37 62 773.1 0 62 38 62 773.1 0 62 39 54 773.1 0 54 40 59 773.1 0 59 41 58 773.1 0 58 42 58 773.1 0 58 43 50 773.1 0 50 44 49 773.1 0 49 45 65 773.1 0 65 46 72 773.1 0 72 47 65 773.1 0 65 48 71 773.1 0 71 49 66 773.1 0 66 50 85 773.1 0 85 51 54 773.1 0 54 52 78 773.1 0 78 53 74 773.1 0 74 54 64 773.1 0 64 55 71 773.1 0 71 56 96 773.1 0 96 57 49 773.1 0 49 58 77 773.1 0 77 59 60 773.1 0 60 60 75 773.1 0 75 61 77 773.1 0 77 62 80 773.1 0 80 63 78 773.1 0 78 64 110 773.1 0 110 65 136 773.1 0 136 66 229 773.1 0 229 67 413 773.1 0 413 68 187 773.1 0 187 69 190 773.1 0 190 70 529 773.1 0 529 71 319 773.1 0 319 72 265 773.1 0 265 73 208 773.1 0 208 74 174 773.1 0 174 75 234 773.1 0 234 76 258 773.1 0 258 77 398 773.1 0 398 78 589 773.1 0 589 79 1092 773.1 0 1092 80 18526 773.1 0 18526 81 17096 773.1 0 17096 82 18979 773.1 0 18979 83 181 773.1 0 181 84 150 773.1 0 150 85 91 773.1 0 91 86 136 773.1 0 136 87 101 773.1 0 101 88 242 773.1 0 242 89 96 773.1 0 96 90 46 773.1 0 46 91 47 773.1 0 47 92 46 773.1 0 46 93 84 773.1 0 84 94 58 773.1 0 58 95 88 773.1 0 88 96 105 773.1 0 105 97 81 773.1 0 81 98 34 773.1 0 34 99 60 773.1 0 60 100 38 773.1 0 38 101 42 773.1 0 42 102 50 773.1 0 50 103 50 773.1 0 50 104 35 773.1 0 35 105 41 773.1 0 41 106 48 773.1 0 48 107 48 773.1 0 48 108 43 773.1 0 43 109 46 773.1 0 46 110 57 773.1 0 57 111 54 773.1 0 54 112 52 773.1 0 52 113 63 773.1 0 63 114 52 773.1 0 52 115 34 773.1 0 34 116 41 773.1 0 41 117 82 773.1 0 82 118 97 773.1 0 97 119 165 773.1 0 165 120 202 773.1 0 202 121 246 773.1 0 246 122 250 773.1 0 250 123 68 773.1 0 68 124 110 773.1 0 110 125 109 773.1 0 109 126 112 773.1 0 112 127 203 773.1 0 203 128 184 773.1 0 184 129 287 773.1 0 287 130 56 773.1 0 56 131 57 773.1 0 57 132 70 773.1 0 70 133 64 773.1 0 64 134 43 773.1 0 43 135 75 773.1 0 75 136 51 773.1 0 51 137 62 773.1 0 62 138 64 773.1 0 64 139 100 773.1 0 100 140 99 773.1 0 99 141 107 773.1 0 107 142 96 773.1 0 96 143 132 773.1 0 132 144 170 773.1 0 170 145 102 773.1 0 102 146 384 773.1 0 384 147 107 773.1 0 107 148 111 773.1 0 111 149 139 773.1 0 139 150 124 773.1 0 124 151 64 773.1 0 64 152 63 773.1 0 63 153 59 773.1 0 59 154 71 773.1 0 71 155 70 773.1 0 70 156 39 773.1 0 39 157 43 773.1 0 43 158 52 773.1 0 52 159 52 773.1 0 52 160 53 773.1 0 53 161 54 773.1 0 54 162 65 773.1 0 65 163 48 773.1 0 48 164 77 773.1 0 77 165 51 773.1 0 51 166 76 773.1 0 76 167 84 773.1 0 84 168 335 773.1 0 335 169 121 773.1 0 121 170 69 773.1 0 69 171 87 773.1 0 87 172 82 773.1 0 82 173 102 773.1 0 102 174 174 773.1 0 174 175 303 773.1 0 303 176 112 773.1 0 112 177 143 773.1 0 143 178 156 773.1 0 156 179 91 773.1 0 91 180 108 773.1 0 108 181 154 773.1 0 154 182 160 773.1 0 160 183 173 773.1 0 173 184 131 773.1 0 131 185 288 773.1 0 288 186 303 773.1 0 303 187 395 773.1 0 395 188 4047 773.1 0 4047 189 10143 773.1 0 10143 190 10252 773.1 0 10252 191 798 773.1 0 798 192 600 773.1 0 600 193 578 773.1 0 578 194 283 773.1 0 283 195 114 773.1 0 114 196 156 773.1 0 156 197 70 773.1 0 70 198 68 773.1 0 68 199 78 773.1 0 78 200 368 773.1 0 368 201 83 773.1 0 83 202 77 773.1 0 77 203 81 773.1 0 81 204 115 773.1 0 115 205 138 773.1 0 138 206 1303 773.1 0 1303 207 1199 773.1 0 1199 208 1830 773.1 0 1830 209 273 773.1 0 273 210 96 773.1 0 96 211 427 773.1 0 427 212 585 773.1 0 585 213 794 773.1 0 794 214 201 773.1 0 201 215 50 773.1 0 50 216 50 773.1 0 50 217 55 773.1 0 55 218 60 773.1 0 60 219 51 773.1 0 51 220 47 773.1 0 47 221 63 773.1 0 63 222 76 773.1 0 76 223 76 773.1 0 76 224 53 773.1 0 53 225 77 773.1 0 77 226 56 773.1 0 56 227 87 773.1 0 87 228 100 773.1 0 100 229 132 773.1 0 132 230 129 773.1 0 129 231 127 773.1 0 127 232 68 773.1 0 68 233 86 773.1 0 86 234 82 773.1 0 82 235 106 773.1 0 106 236 65 773.1 0 65 237 91 773.1 0 91 238 70 773.1 0 70 239 87 773.1 0 87 240 94 773.1 0 94 241 114 773.1 0 114 242 83 773.1 0 83 243 72 773.1 0 72 244 79 773.1 0 79 245 77 773.1 0 77 246 83 773.1 0 83 247 107 773.1 0 107 248 145 773.1 0 145 249 83 773.1 0 83 250 92 773.1 0 92 251 103 773.1 0 103 252 127 773.1 0 127 253 101 773.1 0 101 254 82 773.1 0 82 255 150 773.1 0 150 256 175 773.1 0 175 257 198 773.1 0 198 258 150 773.1 0 150 259 95 773.1 0 95 260 100 773.1 0 100 261 94 773.1 0 94 262 107 773.1 0 107 263 81 773.1 0 81 264 70 773.1 0 70 265 80 773.1 0 80 266 111 773.1 0 111 267 124 773.1 0 124 268 287 773.1 0 287 269 437 773.1 0 437 270 362 773.1 0 362 271 2083 773.1 0 2083 272 2310 773.1 0 2310 273 2506 773.1 0 2506 274 553 773.1 0 553 275 113 773.1 0 113 276 101 773.1 0 101 277 92 773.1 0 92 278 123 773.1 0 123 279 125 773.1 0 125 280 156 773.1 0 156 281 82 773.1 0 82 282 832 773.1 0 832 283 809 773.1 0 809 284 1178 773.1 0 1178 285 72 773.1 0 72 286 60 773.1 0 60 287 103 773.1 0 103 288 132 773.1 0 132 289 98 773.1 0 98 290 99 773.1 0 99 291 155 773.1 0 155 292 307 773.1 0 307 293 345 773.1 0 345 294 325 773.1 0 325 295 186 773.1 0 186 296 108 773.1 0 108 297 112 773.1 0 112 298 98 773.1 0 98 299 117 773.1 0 117 300 238 773.1 0 238 301 454 773.1 0 454 Finished in 17.92 s (73 us/read; 0.82 M reads/minute). === Summary === Total reads processed: 246,165 Reads with adapters: 65,850 (26.8%) Reads that were too short: 231 (0.1%) Reads written (passing filters): 65,619 (26.7%) Total basepairs processed: 61,239,355 bp Total written (filtered): 17,908,503 bp (29.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 65850 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 25 0.0 3 0 0 0 1 24 18 30 0.0 3 0 0 23 5 2 19 665 0.0 3 0 541 59 46 19 20 64476 0.0 4 61065 2743 487 143 38 21 166 0.0 4 27 123 9 3 4 22 14 0.0 4 3 1 7 2 1 23 187 0.0 4 1 1 0 132 53 24 7 0.0 4 0 0 0 1 6 27 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 32 1 0.0 4 1 35 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 55 9 0.0 4 0 0 0 0 9 58 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 2 0.0 4 0 0 0 0 2 89 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 1 95 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 123 2 0.0 4 0 1 0 0 1 127 7 0.0 4 0 0 0 0 7 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 130 16 0.0 4 0 0 0 0 16 133 1 0.0 4 0 0 0 0 1 138 5 0.0 4 0 0 0 0 5 139 1 0.0 4 0 0 0 0 1 142 2 0.0 4 0 0 0 0 2 147 1 0.0 4 0 0 0 0 1 153 2 0.0 4 0 0 0 0 2 154 1 0.0 4 0 0 0 0 1 155 96 0.0 4 0 0 0 4 92 156 51 0.0 4 0 0 1 2 48 157 9 0.0 4 0 0 0 0 9 158 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 197 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 3 0.0 4 0 0 0 0 3 208 3 0.0 4 0 0 0 1 2 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 3 0.0 4 0 0 0 0 3 232 2 0.0 4 0 0 0 1 1 233 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 253 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 262 4 0.0 4 0 0 0 0 4 263 3 0.0 4 0 0 0 0 3 264 2 0.0 4 0 0 0 0 2 265 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 269 1 0.0 4 0 0 0 0 1 271 1 0.0 4 0 0 0 0 1 281 2 0.0 4 0 0 0 0 2 282 6 0.0 4 0 0 0 0 6 283 1 0.0 4 0 0 0 0 1 286 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.08 s (62 us/read; 0.96 M reads/minute). === Summary === Total reads processed: 65,619 Reads with adapters: 11,606 (17.7%) Reads written (passing filters): 65,619 (100.0%) Total basepairs processed: 17,908,503 bp Total written (filtered): 17,778,431 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 11606 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 5.5% G: 90.9% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 64 1025.3 0 64 4 13 256.3 0 13 5 2 64.1 0 2 6 1 16.0 0 1 7 1 4.0 0 1 8 1 1.0 0 1 9 110 0.3 0 4 106 10 4210 0.1 1 228 3982 11 7081 0.0 1 194 6887 12 57 0.0 1 0 57 13 10 0.0 1 0 10 14 7 0.0 1 0 7 15 1 0.0 1 0 1 123 8 0.0 1 7 1 151 21 0.0 1 16 5 152 2 0.0 1 2 153 2 0.0 1 1 1 190 2 0.0 1 0 2 201 1 0.0 1 1 209 10 0.0 1 9 1 226 1 0.0 1 1 227 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.