This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 151.06 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 3,166,434 Reads with adapters: 258,698 (8.2%) Reads that were too short: 19,856 (0.6%) Reads written (passing filters): 238,842 (7.5%) Total basepairs processed: 953,096,634 bp Total written (filtered): 60,394,652 bp (6.3%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 258698 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1390 773.1 0 1390 7 67815 773.1 0 67815 8 78281 773.1 0 78281 9 79 773.1 0 79 10 48 773.1 0 48 11 35 773.1 0 35 12 40 773.1 0 40 13 29 773.1 0 29 14 33 773.1 0 33 15 25 773.1 0 25 16 31 773.1 0 31 17 32 773.1 0 32 18 44 773.1 0 44 19 53 773.1 0 53 20 42 773.1 0 42 21 42 773.1 0 42 22 49 773.1 0 49 23 48 773.1 0 48 24 46 773.1 0 46 25 38 773.1 0 38 26 33 773.1 0 33 27 50 773.1 0 50 28 48 773.1 0 48 29 140 773.1 0 140 30 56 773.1 0 56 31 48 773.1 0 48 32 51 773.1 0 51 33 49 773.1 0 49 34 41 773.1 0 41 35 108 773.1 0 108 36 45 773.1 0 45 37 49 773.1 0 49 38 50 773.1 0 50 39 47 773.1 0 47 40 59 773.1 0 59 41 35 773.1 0 35 42 65 773.1 0 65 43 44 773.1 0 44 44 27 773.1 0 27 45 54 773.1 0 54 46 52 773.1 0 52 47 45 773.1 0 45 48 146 773.1 0 146 49 52 773.1 0 52 50 118 773.1 0 118 51 52 773.1 0 52 52 48 773.1 0 48 53 48 773.1 0 48 54 43 773.1 0 43 55 49 773.1 0 49 56 62 773.1 0 62 57 38 773.1 0 38 58 42 773.1 0 42 59 40 773.1 0 40 60 64 773.1 0 64 61 71 773.1 0 71 62 71 773.1 0 71 63 54 773.1 0 54 64 86 773.1 0 86 65 142 773.1 0 142 66 177 773.1 0 177 67 390 773.1 0 390 68 184 773.1 0 184 69 167 773.1 0 167 70 585 773.1 0 585 71 358 773.1 0 358 72 259 773.1 0 259 73 222 773.1 0 222 74 156 773.1 0 156 75 211 773.1 0 211 76 218 773.1 0 218 77 310 773.1 0 310 78 439 773.1 0 439 79 845 773.1 0 845 80 17232 773.1 0 17232 81 15933 773.1 0 15933 82 18214 773.1 0 18214 83 101 773.1 0 101 84 98 773.1 0 98 85 49 773.1 0 49 86 38 773.1 0 38 87 51 773.1 0 51 88 228 773.1 0 228 89 47 773.1 0 47 90 30 773.1 0 30 91 26 773.1 0 26 92 48 773.1 0 48 93 43 773.1 0 43 94 25 773.1 0 25 95 48 773.1 0 48 96 46 773.1 0 46 97 40 773.1 0 40 98 33 773.1 0 33 99 39 773.1 0 39 100 38 773.1 0 38 101 28 773.1 0 28 102 29 773.1 0 29 103 56 773.1 0 56 104 33 773.1 0 33 105 29 773.1 0 29 106 34 773.1 0 34 107 32 773.1 0 32 108 35 773.1 0 35 109 40 773.1 0 40 110 29 773.1 0 29 111 48 773.1 0 48 112 37 773.1 0 37 113 32 773.1 0 32 114 26 773.1 0 26 115 18 773.1 0 18 116 26 773.1 0 26 117 31 773.1 0 31 118 47 773.1 0 47 119 81 773.1 0 81 120 97 773.1 0 97 121 149 773.1 0 149 122 142 773.1 0 142 123 41 773.1 0 41 124 61 773.1 0 61 125 68 773.1 0 68 126 65 773.1 0 65 127 206 773.1 0 206 128 138 773.1 0 138 129 210 773.1 0 210 130 47 773.1 0 47 131 44 773.1 0 44 132 31 773.1 0 31 133 51 773.1 0 51 134 48 773.1 0 48 135 62 773.1 0 62 136 23 773.1 0 23 137 32 773.1 0 32 138 49 773.1 0 49 139 83 773.1 0 83 140 48 773.1 0 48 141 55 773.1 0 55 142 72 773.1 0 72 143 70 773.1 0 70 144 142 773.1 0 142 145 74 773.1 0 74 146 318 773.1 0 318 147 64 773.1 0 64 148 74 773.1 0 74 149 68 773.1 0 68 150 92 773.1 0 92 151 44 773.1 0 44 152 52 773.1 0 52 153 45 773.1 0 45 154 39 773.1 0 39 155 36 773.1 0 36 156 34 773.1 0 34 157 37 773.1 0 37 158 44 773.1 0 44 159 41 773.1 0 41 160 38 773.1 0 38 161 47 773.1 0 47 162 44 773.1 0 44 163 40 773.1 0 40 164 52 773.1 0 52 165 55 773.1 0 55 166 56 773.1 0 56 167 70 773.1 0 70 168 361 773.1 0 361 169 126 773.1 0 126 170 42 773.1 0 42 171 65 773.1 0 65 172 61 773.1 0 61 173 83 773.1 0 83 174 105 773.1 0 105 175 269 773.1 0 269 176 84 773.1 0 84 177 91 773.1 0 91 178 97 773.1 0 97 179 64 773.1 0 64 180 80 773.1 0 80 181 122 773.1 0 122 182 125 773.1 0 125 183 98 773.1 0 98 184 136 773.1 0 136 185 238 773.1 0 238 186 266 773.1 0 266 187 299 773.1 0 299 188 3787 773.1 0 3787 189 8838 773.1 0 8838 190 9639 773.1 0 9639 191 642 773.1 0 642 192 280 773.1 0 280 193 219 773.1 0 219 194 144 773.1 0 144 195 79 773.1 0 79 196 101 773.1 0 101 197 45 773.1 0 45 198 35 773.1 0 35 199 53 773.1 0 53 200 378 773.1 0 378 201 51 773.1 0 51 202 40 773.1 0 40 203 52 773.1 0 52 204 53 773.1 0 53 205 73 773.1 0 73 206 878 773.1 0 878 207 825 773.1 0 825 208 1367 773.1 0 1367 209 222 773.1 0 222 210 43 773.1 0 43 211 139 773.1 0 139 212 178 773.1 0 178 213 262 773.1 0 262 214 80 773.1 0 80 215 42 773.1 0 42 216 28 773.1 0 28 217 51 773.1 0 51 218 69 773.1 0 69 219 40 773.1 0 40 220 40 773.1 0 40 221 43 773.1 0 43 222 42 773.1 0 42 223 31 773.1 0 31 224 37 773.1 0 37 225 42 773.1 0 42 226 45 773.1 0 45 227 66 773.1 0 66 228 47 773.1 0 47 229 65 773.1 0 65 230 62 773.1 0 62 231 66 773.1 0 66 232 41 773.1 0 41 233 46 773.1 0 46 234 32 773.1 0 32 235 45 773.1 0 45 236 47 773.1 0 47 237 57 773.1 0 57 238 37 773.1 0 37 239 38 773.1 0 38 240 44 773.1 0 44 241 55 773.1 0 55 242 55 773.1 0 55 243 38 773.1 0 38 244 41 773.1 0 41 245 39 773.1 0 39 246 37 773.1 0 37 247 65 773.1 0 65 248 65 773.1 0 65 249 36 773.1 0 36 250 65 773.1 0 65 251 68 773.1 0 68 252 74 773.1 0 74 253 95 773.1 0 95 254 54 773.1 0 54 255 61 773.1 0 61 256 115 773.1 0 115 257 104 773.1 0 104 258 85 773.1 0 85 259 78 773.1 0 78 260 49 773.1 0 49 261 63 773.1 0 63 262 64 773.1 0 64 263 60 773.1 0 60 264 50 773.1 0 50 265 57 773.1 0 57 266 84 773.1 0 84 267 77 773.1 0 77 268 134 773.1 0 134 269 217 773.1 0 217 270 187 773.1 0 187 271 2034 773.1 0 2034 272 2260 773.1 0 2260 273 2457 773.1 0 2457 274 383 773.1 0 383 275 52 773.1 0 52 276 65 773.1 0 65 277 76 773.1 0 76 278 68 773.1 0 68 279 56 773.1 0 56 280 80 773.1 0 80 281 74 773.1 0 74 282 568 773.1 0 568 283 641 773.1 0 641 284 839 773.1 0 839 285 59 773.1 0 59 286 56 773.1 0 56 287 61 773.1 0 61 288 111 773.1 0 111 289 75 773.1 0 75 290 54 773.1 0 54 291 108 773.1 0 108 292 348 773.1 0 348 293 342 773.1 0 342 294 441 773.1 0 441 295 141 773.1 0 141 296 91 773.1 0 91 297 79 773.1 0 79 298 85 773.1 0 85 299 62 773.1 0 62 300 149 773.1 0 149 301 254 773.1 0 254 Finished in 16.11 s (67 us/read; 0.89 M reads/minute). === Summary === Total reads processed: 238,842 Reads with adapters: 67,629 (28.3%) Reads that were too short: 58 (0.0%) Reads written (passing filters): 67,571 (28.3%) Total basepairs processed: 60,394,652 bp Total written (filtered): 18,696,119 bp (31.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 67629 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 7 0.0 3 0 0 7 16 324 0.0 3 0 316 8 17 66445 0.0 3 65231 1175 34 5 18 116 0.0 3 10 104 2 19 26 0.0 3 3 4 16 3 20 512 0.0 3 479 4 1 28 21 2 0.0 3 1 1 23 4 0.0 3 0 0 0 4 28 1 0.0 3 0 0 0 1 29 1 0.0 3 1 32 2 0.0 3 0 0 0 2 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 52 4 0.0 3 4 53 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 4 0.0 3 0 0 0 4 65 4 0.0 3 0 0 0 4 70 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 86 22 0.0 3 0 0 0 22 87 7 0.0 3 0 0 0 7 88 1 0.0 3 1 89 9 0.0 3 0 0 0 9 91 6 0.0 3 0 0 0 6 92 7 0.0 3 0 0 0 7 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 8 0.0 3 0 0 0 8 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 3 0.0 3 0 0 0 3 110 1 0.0 3 0 0 0 1 111 30 0.0 3 0 0 0 30 113 2 0.0 3 0 0 0 2 114 4 0.0 3 0 0 1 3 119 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 9 0.0 3 0 0 0 9 130 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 151 3 0.0 3 0 0 0 3 156 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 2 0.0 3 0 0 0 2 192 1 0.0 3 0 0 1 202 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 218 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 222 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 282 4 0.0 3 0 0 1 3 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.82 s (57 us/read; 1.06 M reads/minute). === Summary === Total reads processed: 67,571 Reads with adapters: 172 (0.3%) Reads written (passing filters): 67,571 (100.0%) Total basepairs processed: 18,696,119 bp Total written (filtered): 18,695,581 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 172 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 24.4% C: 32.6% G: 23.8% T: 19.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 160 1055.8 0 160 4 10 263.9 0 10 6 1 16.5 0 1 12 1 0.0 1 0 1